Home | Community | Message Board


Original Seeds Store - Cannabis Seeds
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: Agar, Parafilm, Petri Dish

Jump to first unread post. Pages: 1
Invisibleeric_the_red
 User Gallery

Registered: 02/28/03
Posts: 12,831
Loc: happy land
a ? for those who pour their own agar
    #2045142 - 10/26/03 05:49 PM (13 years, 1 month ago)

do you ever pour a bunch of dishes and store them for later use?

it's a pain to get out all the supplies and equipment to make a new batch of agar when you only need one or two dishes.

i was wondering if anyone knows of a good way to make about 100 dishes one day and store them contaminate-free and neatly for up to 1 month.



--------------------


Anno cock? is that some kind of Greek liqueur? -Geo's All Knowing Sex Slave


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: a ? for those who pour their own agar [Re: eric_the_red]
    #2045164 - 10/26/03 05:55 PM (13 years, 1 month ago)

They tend to dry up over time. Sealed with parafilm, they remain contaminant free, and usuable for a month no problem. They start drying up after monthS in storage. Pour them thicker then normal and you should have no problems.

Cultures poured thick and removed from cold storage after 2 years were still usuble by the emerging mycelium coming out of hibernation. Shallow pours crack after a few months to six months.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: a ? for those who pour their own agar [Re: ]
    #2045182 - 10/26/03 06:02 PM (13 years, 1 month ago)

Petries sealed (parafilm or saran wrap) should keep in the fridge for at least a year....

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleeric_the_red
 User Gallery

Registered: 02/28/03
Posts: 12,831
Loc: happy land
Re: a ? for those who pour their own agar [Re: ]
    #2045188 - 10/26/03 06:04 PM (13 years, 1 month ago)

thanks for the quick answer.

i think i might build some type of little cabinet to store a bunch of ready-to-go dishes.



--------------------


Anno cock? is that some kind of Greek liqueur? -Geo's All Knowing Sex Slave


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: a ? for those who pour their own agar [Re: eric_the_red]
    #2045214 - 10/26/03 06:16 PM (13 years, 1 month ago)

I'd really put them in a fridge or somewhere cool for long-term storage.... Just me.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleYidakiMan
Stranger
Male User Gallery
Registered: 09/29/02
Posts: 2,023
Re: a ? for those who pour their own agar [Re: micro]
    #2045330 - 10/26/03 07:12 PM (13 years, 1 month ago)

I prepare two liters at a time. I normally get 30 dishes out of a liter. I store the extra plates on the very top shelf of the fridge to keep them cleaner.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleeric_the_red
 User Gallery

Registered: 02/28/03
Posts: 12,831
Loc: happy land
Re: a ? for those who pour their own agar [Re: micro]
    #2045412 - 10/26/03 07:50 PM (13 years, 1 month ago)

they will go in the fridge. i just want to find or make some kind of container that they will fit securely into, so that they won't slide around, fall over, and get contaminated.

maybe something like this:


--------------------


Anno cock? is that some kind of Greek liqueur? -Geo's All Knowing Sex Slave


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 10 days
Re: a ? for those who pour their own agar [Re: micro]
    #2046448 - 10/27/03 03:47 AM (13 years, 1 month ago)

>I'd really put them in a fridge or somewhere cool for long-term storage

Why fridge?
If they are sterile and sealed and stored at room temperature, they won?t spoil and you at least are assured they are clean.
If you put them in fridge, you could hide a potentional dormant contamination that could break out once you inoculate the dishes and incubate them.

I store them at room temperature for that reason.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleZildjian
human

Registered: 09/11/02
Posts: 208
Loc: new zealand
Re: a ? for those who pour their own agar [Re: Anno]
    #2046631 - 10/27/03 06:24 AM (13 years, 1 month ago)

i store mine in the incubator because the teperature doesn't fluctuate in there. if sealed with plastic wrap, they don't dry out and the steady temp prevents condensation from forming inside the dishes.

yeah, for some reason at room temperature, condensation forms on the inside of the petri dish.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePaid
Pict
 User Gallery

Registered: 03/13/03
Posts: 5,376
Loc: Zone ate
Last seen: 12 years, 9 months
Re: a ? for those who pour their own agar [Re: eric_the_red]
    #2046666 - 10/27/03 07:11 AM (13 years, 1 month ago)

If you wantto store uninoculated plates for
long its worth adding a bit of h2o2 imo.


--------------------



Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: a ? for those who pour their own agar [Re: Paid]
    #2046739 - 10/27/03 08:30 AM (13 years, 1 month ago)

I work in a lab; we always store them in the fridge. Anno -- that makes sense, too, but it's just never the way I've done things -- always thought it a contamination risk. I always leave them out for like a week or so to make sure they're clean, but then I put them in the fridge just in case....

Leaving them out can't really hurt, though, if they're sterile and don't dry out....

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: a ? for those who pour their own agar [Re: micro]
    #2090618 - 11/10/03 10:25 PM (13 years, 27 days ago)

This is an old post but I make up the dishes and wrap four at a time in parafilm. Then place 4 stacks of 4 dishes inside the polly bag and then seal with parafilm. They then stack up realy nicly bag ontop of bag in the closet.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinebonsai
shaman intraining

Registered: 05/21/03
Posts: 169
Loc: Somewhere in a desert
Last seen: 11 years, 2 months
Re: a ? for those who pour their own agar [Re: ]
    #2094378 - 11/11/03 11:21 PM (13 years, 26 days ago)

I make up 2 liters at a time which gives me about 80 dishes (100mm). Then seal with parafilm. I then put 20 dishes in a gallon zip lock bag and seal that. I leave them out for a week or two. Then in the fridge they go. Never had a problem. I have not done any longevity tests but all have produced.
bonsai


--------------------
"Without deviation from the norm, progress is not possible."
--Frank Zappa--

:laugh:


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: Agar, Parafilm, Petri Dish

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Is it necessary to dry agar plates? shymanta 8,269 19 07/07/15 10:59 PM
by Demonsmoke
* Agar plates dog 826 3 04/05/05 11:45 AM
by Arp
* Questions On Agar ParticleMan 2,338 13 03/28/02 12:11 AM
by Shaw
* to pour then pc petris or steralize then pour? Gr0wer 1,031 14 04/27/04 04:12 PM
by Joshua
* Re: Dehydration of Agar Cultures? Anonymous 1,125 7 02/09/00 07:34 PM
by Odin 13
* Alternatives to Agar Plates?
( 1 2 all )
GGreatOne234 3,140 20 09/11/02 09:14 AM
by GGreatOne234
* 3 week old agar still good? johnny_poke 1,332 16 01/14/09 10:11 PM
by didjin_d
* P. Azurescens Specialty Agar/Broth buddig 1,382 6 06/01/02 09:13 AM
by buddig

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
754 topic views. 1 members, 3 guests and 4 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Lil Shop Of Spores
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.004 seconds on 14 queries.