|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Abshroom
Cause he can


Registered: 06/05/14
Posts: 810
Loc: Germany
|
|
hey guys  started my first try with A2G today (inspired by SpitballJedi, thanks mate ) here's one of the jars:
 probably you immediately see the problem? the agar wedge sticks at the lid and even won't come down after heavy shaking  do you have some tips how to bring it down without opening the jars?
|
wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,918
|
Re: Agar to Grain (A2G) [Re: Abshroom]
#20436985 - 08/18/14 11:31 AM (9 years, 5 months ago) |
|
|
Try smacking it lol just don't cut yourself
|
Quexl


Registered: 12/17/13
Posts: 1,443
Loc:
Last seen: 8 years, 11 months
|
Re: Agar to Grain (A2G) [Re: Abshroom]
#20437574 - 08/18/14 02:00 PM (9 years, 5 months ago) |
|
|
Hit the lid with a spoon or something. That's annoying, I've had it happen too. I just crack it open and poke it off with the end of a sterile blade in a sab.
|
tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 17 days
|
Re: Agar to Grain (A2G) [Re: Quexl]
#20437755 - 08/18/14 02:39 PM (9 years, 5 months ago) |
|
|
i's say smack the lid downward opposite the wedge so that the momentum of the smack and the force of gravity can help to pull it away. So position the jar on it's side and try to bang downward with the wedge facing you. Sorry but i can't think of a way to describe it better hope you understand as that has helped me in your situation. If not quexi's idea is all that's left.
--------------------
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
|
looking pretty good. are you getting excited? spawning is always fun.
|
SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
|
|
lol...not as excited as I used to get. Especially when I used to have contam issues.
But, I do get a lot of joy seeing something I created living and growing.
I played with some rye this weekend like we talked about and the results are promising. Grain Prep Experiments Your 2 cents is always welcome. It's a journal.
I also updated the OP to be more like a tek and added this:
Be careful when dropping in your wedge. These things really stick the glass and are almost imposable to get unstuck. You don't have to shake the jar right away. What I do is, once the lid is closed, I roll it around a little to get some grains to cover it. Then, I just let it be. It doesn't really matter if the agar sticks to the glass as long as it's covered with some grain.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
|
I don't shake my agar inoculated jars until after the mycelium has moved into the grain and the agar has dried out a bit.
|
SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
|
Re: Agar to Grain (A2G) [Re: MudaFuka]
#20438678 - 08/18/14 06:13 PM (9 years, 5 months ago) |
|
|
That's been my experience as well. But, I don't really shake after inoculation, it's more of a "roll it around a bit" because I usually get the wedge stuck somewhere I don't want it with shaking.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
|
i tilt the jar as i drop it in, so when the jar gets put upright, the grains roll over the wedge, bringing it under as they go. sometimes no matter what i do, it will get stuck like that, so i will lean/tilt the jar against the shelf wall, so the grains are covering the stuck wedge. then after a few days, shake after it leaps off and the wedge dries up, and it comes right off.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
TaiJi
Stranger in a strange land

Registered: 07/16/14
Posts: 148
Last seen: 9 years, 4 months
|
|
Quote:
blindingleaf said: i tilt the jar as i drop it in, so when the jar gets put upright, the grains roll over the wedge, bringing it under as they go. sometimes no matter what i do, it will get stuck like that, so i will lean/tilt the jar against the shelf wall, so the grains are covering the stuck wedge. then after a few days, shake after it leaps off and the wedge dries up, and it comes right off.
Oh, I like this.
-------------------- "If some study enlightenment, we study illusion. We seek medicine in the very poison that has seduced us. The mind, we say, is too much with us, so let's heap on some more. The left-hand work. Whatever." -Dale Pendell
|
Quexl


Registered: 12/17/13
Posts: 1,443
Loc:
Last seen: 8 years, 11 months
|
|
Yeah, I do the same thing.
|
Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,865
Last seen: 17 hours, 49 minutes
|
|
Quote:
Inocuole said:
Quote:
Buck513 said: I knew isolation took time, I was just under the impression it was taking an isolate, then fruiting them and continuing to take isolates of the best fruits. I could be dead wrong I gotta read up. I was gonna be doin my agar usin pastys tek too
You can't just "take an isolate", you have to do upwards of 10 transfers at least to get an actual isolate/monoculture (not counting clones), otherwise you're just working with limited genetics, or a clone, which is not the same as a monoculture, since a single mushroom can have hundreds of different genetics.
It depends on the method you use. Isolation streaking allows you to find separated individual strains on the first plate or even monokaryotic mycelia.
Quote:
SpitballJedi said:
That's been my experience as well. But, I don't really shake after inoculation, it's more of a "roll it around a bit" because I usually get the wedge stuck somewhere I don't want it with shaking.
So true and then when you try to get it off it winds up in an even worse place 
I know it's common practice here but scalpels are generally used for removing a wedge for microscopy. Trouble getting the wedge into the jar, and the need to use large wedges to compensate for only having a single inoculation point are problems associated with using a scalpel instead of an inoculation loop/hook.
--------------------
|
tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 17 days
|
Re: Agar to Grain (A2G) [Re: Kizzle]
#20441128 - 08/19/14 05:16 AM (9 years, 5 months ago) |
|
|
when using large wedge or whole plate (pastyplates and other no pours because the size of a whole petri dish is too big of a wedge to transfer into a jar and the extra amount won't speed things up much) 1.slide scalpel alongside of agar and underneath then begin to pry upward 2. slide scalpel flat underneath all the way to the middle of the agar and then gently stab upward past the center. 3. Balance agar on scalpel then lift and drop into receiving jar. that's the easiest for me and works every time though i may pour my dishes a little deep which might make this easier and maybe the fact i use slightly less water in my agar as well for stiffness as i had a couple batches turn out a bit gel like. I did drop a whole plate once so not everything is perfect. maybe i should try the inoculation loop idea just to see how i like it. at first I tried stabbing from the top and failed miserably and ended demolishing most of the wedge in the process from it sliding off. stabbing underneath the wedge and balancing was incredibly easier, though could definitely have more room for error by touching more of the scalpel. Is that how most others do it, too?
|
|