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OfflineRoseM
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Don Quixote Needs MycoBag Help
    #2042439 - 10/25/03 04:26 PM (20 years, 5 months ago)

Well, Don Quixote is a little perplexed. He often is.

He had a MycoBag that started to colonize quickly then, it appeared like the myc turned a little grey. Seems the bag was either too dry, or it fully colonized with cottony growth and Don Didn't remove it from the incubator fast enough.

Either that, or it had cobweb. Only the grey looking fluff leads me to think it might have been cobweb, there were no weblike contams. Only little fluffy greyish white hair.

The bag dried out very fast. The cottony grey growth had spread very quickly too.

Don removed it from the incubator and watched the bag. Two days ago, while checking the bag, Don said he could shake it like dry dirt.

He injected the bag with 24cc of water and 5cc of h2o2 and shook.

Two days later, the bag has obvious white growth all the way through it, and all the grains have superglued together.

Please confirm if the cottony bag is mycelia. It sure looks good, but this bag was grey before. The mycelia doesn't seem to want to stick to the bag.

The top, which you can't see, is almost totally white.





Don hasen't encountered much cottony growth in mycobags. Usually it is rizo.

These two photos show rizo growth. It loves growing on the bag itself.





Bags are hard to photograph well.

Has anyone else rehydrated a bag before full colonization?


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InvisibleHanky
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Re: Don Quixote Needs MycoBag Help [Re: Rose]
    #2042452 - 10/25/03 04:32 PM (20 years, 5 months ago)

i've rehydrated a few cakes that got half baked by a heater pad and noticed that the myc growth was a lot fluffier after.


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Coaster is an idiot...
[quote]Coaster said:
but i thnk everything thats pure is white?
[/quote]



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OfflineRoseM
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Re: Don Quixote Needs MycoBag Help [Re: Hanky]
    #2042464 - 10/25/03 04:38 PM (20 years, 5 months ago)

Pictures added to the first post.


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OfflineRoseM
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Re: Don Quixote Needs MycoBag Help [Re: Rose]
    #2042476 - 10/25/03 04:41 PM (20 years, 5 months ago)

Will the cottony bag become fully white like the rizo bag? How will Don know when it has fully colonized?


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InvisibleHanky
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Re: Don Quixote Needs MycoBag Help [Re: Rose]
    #2042529 - 10/25/03 05:12 PM (20 years, 5 months ago)

that looks just like my jars did after i rehydrated them (i'll try to find a pic). when my jars went like that the myc seemed to move across the surface like a wall,with no rhizo growth.
just wait till it's all white i suppose then add a week to make sure.


--------------------
Coaster is an idiot...
[quote]Coaster said:
but i thnk everything thats pure is white?
[/quote]



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OfflineRoseM
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Re: Don Quixote Needs MycoBag Help [Re: Hanky]
    #2042552 - 10/25/03 05:22 PM (20 years, 5 months ago)

Thanks for the input, Hank.


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OfflineRoseM
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Re: Don Quixote Needs MycoBag Help [Re: Rose]
    #2042580 - 10/25/03 05:39 PM (20 years, 5 months ago)

How much water can you inject into a dry mycobag (ie the verm isn't sticking to the sides anymore) without having to drain it like it was dunked?

The dry bag sure soaked up the 24cc I'm wondering if Don Quixote could have used more.

Is greying a sign of quickly drying substrate? I've never witnessed this.... well until now.


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InvisibleHanky
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Re: Don Quixote Needs MycoBag Help [Re: Rose]
    #2042601 - 10/25/03 05:48 PM (20 years, 5 months ago)

your welcome....heres that pic,you can see where thermal death occured (heater pad fiasco,now i use a magash tub in a tub incubator)
and you can see how the new growth seems to spread across the old myc like a wall/wave. this only happened after i rehydrated..maybe adding water increases the RH inside the jar/bag and causes the same fluffy myc growth that you'd see on birthed cakes?



--------------------
Coaster is an idiot...
[quote]Coaster said:
but i thnk everything thats pure is white?
[/quote]



Edited by hanky (10/25/03 05:49 PM)

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Invisibleplura16
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Re: Don Quixote Needs MycoBag Help [Re: Rose]
    #2042603 - 10/25/03 05:49 PM (20 years, 5 months ago)

Greying is usually a sign of contamination.


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- USA -

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OfflineRoseM
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Re: Don Quixote Needs MycoBag Help [Re: plura16]
    #2042623 - 10/25/03 05:57 PM (20 years, 5 months ago)

It isn't grey any more. I'm sure the bag was cooking a little hot. The fuz started white then turned grey, now, it is obviously myc... just never seen a cotton bag or a dry bag before.


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OfflineRoseM
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Re: Don Quixote Needs MycoBag Help [Re: Rose]
    #2042631 - 10/25/03 06:00 PM (20 years, 5 months ago)

Thanks for the picture Hanky, I'm pretty sure this bag experienced thermal death. Your jar looks very similar to Don's bag except he could shake it up after the death occoured.

Don'll have to move it to a different spot in the incubator... if Don put's in in the incubator again at all.

Good to know it should be fine.


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OfflineRoseM
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Re: Don Quixote Needs MycoBag Help [Re: Rose]
    #2042644 - 10/25/03 06:03 PM (20 years, 5 months ago)

I just realized, the myc isn't growing on the sides of the bag because the verm was so dry, it didn't stick to the walls.

:lol:


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InvisibleHanky
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Re: Don Quixote Needs MycoBag Help [Re: Rose]
    #2042646 - 10/25/03 06:03 PM (20 years, 5 months ago)

i'd recommend making a tub in a tub if you can...see my 7day jar post in the growlog and picture forum...these things work great.


--------------------
Coaster is an idiot...
[quote]Coaster said:
but i thnk everything thats pure is white?
[/quote]



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OfflineRoseM
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Re: Don Quixote Needs MycoBag Help [Re: Hanky]
    #2042666 - 10/25/03 06:13 PM (20 years, 5 months ago)

Well, Don is in no hurry. He doesn't mind colonizing at room temperature.

The tub is an investment, Don Quixote can't afford to make.

Don has minimal space and maximum patience.


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OfflineRoseM
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Re: Don Quixote Needs MycoBag Help [Re: Rose]
    #2042735 - 10/25/03 06:45 PM (20 years, 5 months ago)

On further inspection of the bag, Don Quixote noticed the bag is leaking air. He injected more water and h2o2 inside. The bag has a hole near the filter... allowing Don to smell the bag. Smells like mycobag.

He'll keep me posted on how it goes.

Finally, the verm seems rehydrated.


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OfflineRoseM
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Re: Don Quixote Needs MycoBag Help [Re: Rose]
    #2059685 - 10/31/03 01:56 AM (20 years, 4 months ago)

Update and question.

The dry bag is doing great after Don injected more h2o and h2o2 and shook the bag. Don wonders when this bag can be cold shocked and fruited.

How's it look?



There is myc all the way through it. The picture doesn't do it justice. It just is very cottony. It gets whiter every day. Don imagines this bag colonized more like WBS would because it dried so much, he could literally shake the bag like a shake and bake... this must've spread the mycelium well. Also, since the bag has a small hole near the filter, it is a good thing the substrate is covered with myc. Otherwize this bag would be a contamination waiting to happen. Some rizo growth is visible.

I'm used to Don's bags looking like this.



It is easy to tell when this type of bag^ can be fruited.

Any advice on the top pic? The myc is still building a network but since primordial knots are forming on top, I'm wondering if it could be fruited now.

How will Don know this bag is ready? Will it become all white, like the second picture? Again, the top bag is already covered with cottony growth.

A prompt reply would be great. Because the bag is leaking, Don wants to get it out of the incubator as soon as possible. Otherwise, he will need to add more water eventually.

Thanks


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InvisibleHanky
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Re: Don Quixote Needs MycoBag Help [Re: Rose]
    #2059768 - 10/31/03 02:52 AM (20 years, 4 months ago)

were you planing to case it?
if so you'd be fine to case it now.


--------------------
Coaster is an idiot...
[quote]Coaster said:
but i thnk everything thats pure is white?
[/quote]



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OfflineRoseM
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Re: Don Quixote Needs MycoBag Help [Re: Hanky]
    #2059781 - 10/31/03 03:12 AM (20 years, 4 months ago)

Yeah this bag would be perfect to case. Too bad Don Quixote is so thick skulled. He insists on conquering agar before he attempts casing... sigh. Don and his dumb quests.

No casing for Don Quixote. He desparately needs prints from this grow... and they don't get much more sterile than invitro... :smile:  Perhaps, after the first flush, but no sooner. He realy plans on fruiting as cakes after the first flush... a little MycoBag dunk 'n roll. He hasn't tried dunking or rolling yet... God! I'm sick and tired of telling Don about all these things I've read at The Shroomery. He should get an account and ask his own damn questions... but he's (conviently) computer phobic.

I gave Don my Corn bag which I won in a MycoBag contest. He plans on inoculating and casing it soon.

I suppose, this bag is good to be removed from the incubator... it'll continue to colonize without the extra heat... and it will retain water.

But, I'm still wondering how long this one will take. I've never seen anything like it. Two weeks ago, it was dry and overcooked. I almost told DQ to throw it out because I thought it was cobweb.

:blush:

Now it is racing to catch up with Don's strongest bag.


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OfflineRoseM
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Re: Don Quixote Needs MycoBag Help [Re: Rose]
    #2059804 - 10/31/03 03:43 AM (20 years, 4 months ago)

Doesn't that top bag look like a casing in a bag?

Wow.

Now that I realy look at that top photo objectively, it looks like all those photos of casings that are getting ready to pin. Is that what is happening in there?

I wonder if the top bag looks fuzzy because it is more rizo than the "Tidal Wave (bottom picture)" white bag from the bottom picture.

Trust me, the "Tidal Wave" bag has some spectacular rizo growth but the top bag has much less of this "Tidal Wave" myc.

Which myc is better?

I've only seen Don's bags and only when he's grown b+.

I'm not firmiliar with other strains.

I think these photos display the difference between two types of mycelial growth from the same strain... from the same print. I'd love to know what I'm looking at.

What differences do you expect Don'll see between bags once they begin to fruit?

Which one do you think will fruit first?

I'm putting my money on the top bag.


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