Home | Community | Message Board

Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Agar

Jump to first unread post. Pages: 1
Registered: 10/25/03
Posts: 1
Last seen: 13 years, 8 months
Questionable sterility of spores
    #2042288 - 10/25/03 05:25 PM (13 years, 8 months ago)


If this subject is addressed somewhere and I missed it, please point me in the right direction! I've been searching the hell out of this site and haven't been able to find any information (though it's probably due to gross incompetence...). I inherited a spore print about a year ago; it's on a piece of weigh paper in an envelope and there's no way in hell it's sterile. I don't want to contaminate my cultures from the get-go!...but I can't think of a way to sterilize the suckers without killing them. I know that many spores are heat-resistant and was wondering if I could flash-pasteurize them or something. Any suggestions would be most welcome! Thanks!

Post Extras: Print Post  Remind Me! Notify Moderator
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,123
Loc: my room
Last seen: 27 days, 19 hours
Re: Questionable sterility of spores [Re: Clouseau]
    #2042423 - 10/25/03 06:19 PM (13 years, 8 months ago)

Start them on agar, then you will see if they are clean or not.

Spores are hardly ever 100.00% sterile, still they can often be used even for direct inoculations via a syringe.

Post Extras: Print Post  Remind Me! Notify Moderator
Devil's Advocate
Female User Gallery

Registered: 09/24/03
Posts: 22,294
Loc: Mod not God Flag
Last seen: 12 days, 17 hours
Trusted Cultivator
Re: Questionable sterility of spores [Re: Anno]
    #2042505 - 10/25/03 06:57 PM (13 years, 8 months ago)

Make multiple syringes and try it on multiple jars... once you get one working jar, you're in business.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Similar ThreadsPosterViewsRepliesLast post
* Thinking about growing, but have a question about spores. shamanamba 571 1 09/06/06 10:30 AM
by RogerRabbit
* Couple quick questions destindiver1980 582 2 03/11/08 03:40 AM
by fiasco
* Weird question concerning contamination and spore print whitelight7 619 2 02/23/09 07:19 PM
by whitelight7
* FC contam question, PLZ HELP nerdiator 861 5 10/01/08 12:38 PM
by nerdiator
* Questionable Liquid Culture Contamination. Day 3 DropDemSh1tz 4,559 2 12/31/06 10:28 AM
by hightimesreader
* Help me protect my B+ jars from contams! How should I sterilize my needle? RobMarley420 841 0 07/19/05 04:38 AM
by RobMarley420
* Sterile inoculation Shroomfobby 717 1 10/21/05 07:23 PM
by RogerRabbit
* spore cloud doze 858 8 10/20/08 11:27 PM
by doze

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Tmethyl, Pastywhyte, bodhisatta
408 topic views. 1 members, 3 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Crestline Sales - MycoPath
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.005 seconds on 19 queries.