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Invisibleblackdust
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Re: P2G(Pf Jar to Grain Transfer) [Re: eatyualive]
    #20751585 - 10/25/14 12:56 PM (10 years, 2 months ago)

Quote:

eatyualive said:
how many quarts volume are the bags total? just do an estimate based on quarts. if i can use 1/2 pint for 10-15 quarts. then whatever volume your bags hold estimate it at that. so if your bag holds 4 quarts. then you can easily do 3-4 of those bags with one 1/2 pint.





If I was to go 1 half pint to a 5 quart bag, would I see a faster colonization time for the bag?

Thanks!

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Invisibleeatyualive
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Re: P2G(Pf Jar to Grain Transfer) [Re: blackdust]
    #20752109 - 10/25/14 03:30 PM (10 years, 2 months ago)

Yes you would see faster colonization. id say a week or less. you may need to shake/mix the bag up. but see how it looks. you may not need to. if you can scrape it into enough small pieces and mix it up very evenly throughout the grain it will colonize rather quickly. i also do the slurry in the bags. where i blend up 1/2 pint pf cake into sterlie water and pour it into the bags.

if your looking for speed and volume then slurry and liquid inoculant are the way to go. here is an example after about 3-4 days. if your a bit new to cultivation you may want to take it one step at at a time. get a few grows in, then try this after you have your clean technique down!



http://www.shroomery.org/forums/showflat.php/Number/10648149


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Invisibleblackdust
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Re: P2G(Pf Jar to Grain Transfer) [Re: eatyualive]
    #20752345 - 10/25/14 04:35 PM (10 years, 2 months ago)

Quote:

eatyualive said:
Yes you would see faster colonization. id say a week or less. you may need to shake/mix the bag up. but see how it looks. you may not need to. if you can scrape it into enough small pieces and mix it up very evenly throughout the grain it will colonize rather quickly. i also do the slurry in the bags. where i blend up 1/2 pint pf cake into sterlie water and pour it into the bags.

if your looking for speed and volume then slurry and liquid inoculant are the way to go. here is an example after about 3-4 days. if your a bit new to cultivation you may want to take it one step at at a time. get a few grows in, then try this after you have your clean technique down!



http://www.shroomery.org/forums/showflat.php/Number/10648149





I'm so going slurry!

:awethumb:

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Invisiblepurpdiv
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Re: P2G(Pf Jar to Grain Transfer) [Re: blackdust]
    #20923074 - 12/03/14 02:56 PM (10 years, 1 month ago)

What would be the optimal conditions for this tek?

TEMP=
LIGHT=

IS THERE A SPECIFIC TEMP PER STRAIN/RACE?


I AM HAVING A HARD TIME GETTING AN LC CULTURE TO GROW FAST ENOUGH, 2 WEEKS INTO IT AND ONLY 2 SMALL COTTONY PUFF BALLS IN SUSPENSION KEPT AT 81F IN AN INCUBATOR.

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InvisiblePastywhyteMDiscord
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Re: P2G(Pf Jar to Grain Transfer) [Re: purpdiv]
    #20923095 - 12/03/14 03:00 PM (10 years, 1 month ago)

Get it out of the incubator, room temp is fine. Chances are its not the temps slowing things up.

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Invisiblepurpdiv
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Re: P2G(Pf Jar to Grain Transfer) [Re: Pastywhyte]
    #20923220 - 12/03/14 03:24 PM (10 years, 1 month ago)

It is quite cold here at the moment, the room stays at about 62F (16.8C) during the day time.

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InvisiblePastywhyteMDiscord
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Re: P2G(Pf Jar to Grain Transfer) [Re: purpdiv]
    #20923378 - 12/03/14 03:55 PM (10 years, 1 month ago)

Then turn the temp down. I would have it at no more than 75. Mycelium growth rates plateau in the high seventies and drop off in the 80's resulting in slow colonization.

However that is probably not why your LC stalled. Contam is more likely.  You should make a thread on the board instead of jacking this one and post a pic if you want a good response.

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Invisiblepurpdiv
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Re: P2G(Pf Jar to Grain Transfer) [Re: Pastywhyte]
    #20923557 - 12/03/14 04:22 PM (10 years, 1 month ago)

Jacking? not my intention.

I Want to use this tek with some cakes currently colonizing(at~70%), but the temps is my concern based on the observations from the LCs experiments(honey,karo,dex-lme) behavior.


Are the mycelia growths temps specific to strains/races?


I have noticed that everybody says "ROOM TEMP" does that refers to STP (32F/0C,1 Atmosphere) in chemistry or not?

Thanks


now is clear.
"Definition: Room temperature, IS A RANGE of temperatures that denote comfortable habitation for humans.

Room temperature can be any temperature between 20 °C/68F and 29 °C/84.2 . 300 K)
"

Edited by purpdiv (12/03/14 05:44 PM)

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Invisiblewowimflabbergasted
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Re: P2G(Pf Jar to Grain Transfer) [Re: purpdiv]
    #20923577 - 12/03/14 04:25 PM (10 years, 1 month ago)

If you are comfortable, your shrooms are comfortable.


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InvisibleSpitballJedi
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Re: P2G(Pf Jar to Grain Transfer) [Re: purpdiv]
    #20923583 - 12/03/14 04:26 PM (10 years, 1 month ago)

"room temperature" means "if you're comfortable in a t-shirt and shorts, then your mushrooms are, too." It applies to all the "strains" of P.Cubensis at all stages of growth.


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Invisibleeatyualive
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Re: P2G(Pf Jar to Grain Transfer) [Re: purpdiv]
    #20924129 - 12/03/14 06:13 PM (10 years, 1 month ago)

Quote:

purpdiv said:
What would be the optimal conditions for this tek?

TEMP=
LIGHT=

IS THERE A SPECIFIC TEMP PER STRAIN/RACE?


I AM HAVING A HARD TIME GETTING AN LC CULTURE TO GROW FAST ENOUGH, 2 WEEKS INTO IT AND ONLY 2 SMALL COTTONY PUFF BALLS IN SUSPENSION KEPT AT 81F IN AN INCUBATOR.





room temps. i usually keep my temp between 76-78F. but i do place all my jars incubating next to each other in a closed shelf.
i just stick all my jars in this at room temp. or on a shelf in a chest of drawers. something that has little airflow in it.






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Edited by eatyualive (12/03/14 06:16 PM)

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Invisiblepurpdiv
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Re: P2G(Pf Jar to Grain Transfer) [Re: eatyualive]
    #20925110 - 12/03/14 09:14 PM (10 years, 1 month ago)

THANKS EAT AND PAST, VERY HELPFUL

Edited by purpdiv (12/03/14 09:19 PM)

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Offlinebburnett227
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Re: P2G(Pf Jar to Grain Transfer) [Re: purpdiv]
    #21924953 - 07/10/15 02:57 PM (9 years, 6 months ago)

How much of the pf cake are you using per grain jar? If I'm understanding this right, the more of the cake u use, the more innoc points u have, but, what's the magic ratio to still have the rapid colonization of the grains but not use your entire cake on one jar?


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Invisibleeatyualive
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Re: P2G(Pf Jar to Grain Transfer) [Re: bburnett227]
    #21927672 - 07/11/15 07:10 AM (9 years, 6 months ago)

Quote:

bburnett227 said:
How much of the pf cake are you using per grain jar? If I'm understanding this right, the more of the cake u use, the more innoc points u have, but, what's the magic ratio to still have the rapid colonization of the grains but not use your entire cake on one jar?



about 1/10th if im doing 10 quarts. really its by eye. and at the end if there is more leftover. i throw all of it into the last jar or two.

no matic formula, usually the pf cake breaks into susch small pieces that even 1/10th of a cake is similar to grain jar g2g.


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Re: P2G(Pf Jar to Grain Transfer) [Re: eatyualive]
    #21928343 - 07/11/15 10:58 AM (9 years, 6 months ago)

Thank you, sir. Gonna do this in a few days and just to be sure I read all this right, I'm not waiting for 100% consolidation, just 100% colonization, or visible growth, right?


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Invisibleeatyualive
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Re: P2G(Pf Jar to Grain Transfer) [Re: bburnett227]
    #21928665 - 07/11/15 12:21 PM (9 years, 6 months ago)

it will not hurt if you use consolidated jars. if you want to be on the safe side. wait until 100% visible colonization. then fork your jars 2-3 days later.

personally, ive never used pf jars that have knotting. so, i would not advise letting the pf jar go that long.


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Re: P2G(Pf Jar to Grain Transfer) [Re: eatyualive]
    #21928866 - 07/11/15 01:21 PM (9 years, 6 months ago)

Thank you very much again.  Will do


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Invisibleeatyualive
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Re: P2G(Pf Jar to Grain Transfer) [Re: bburnett227]
    #21964660 - 07/19/15 12:06 PM (9 years, 5 months ago)

ok so here is an update. this was a multispore pf jar that was inoculated on 7.24.2014. once colonized, the jar was stored in the fridge until a new p2g transfer was done on 7.12.2015 almost a full year later. growth is not as fast but here is 7 days growth. the jars were shaken around day 3. one is finished the rest are almost there.

1 pf jar was p2ged to 10 quarts of wbs grain.

intending to take some clone material to keep this cube variety.



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Offlinebburnett227
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Re: P2G 123 (Pf Jar to Grain Transfer) [Re: eatyualive]
    #21966654 - 07/19/15 06:57 PM (9 years, 5 months ago)

Starting this as we speak. Only downside is I'm gonna be using 16 pint jars since quarts don't fit in the pc. The other alternative thing is I'm using "wild finch blend..." Haven't read too much about this failing.  Other shitty notenote, if ur gonna use wild finch blend, make sure strainer has super small holes...I got bird seed all over my sink right now Lol. Wish me luck!!


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Invisibleeatyualive
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Re: P2G 123 (Pf Jar to Grain Transfer) [Re: bburnett227]
    #21966762 - 07/19/15 07:22 PM (9 years, 5 months ago)

Quote:

bburnett227 said:
Starting this as we speak. Only downside is I'm gonna be using 16 pint jars since quarts don't fit in the pc. The other alternative thing is I'm using "wild finch blend..." Haven't read too much about this failing.  Other shitty notenote, if ur gonna use wild finch blend, make sure strainer has super small holes...I got bird seed all over my sink right now Lol. Wish me luck!!



so you basically have 8 quarts total. that should be good with 1-half pint pf cake.

good luck. and yeah they don't make strainers small enough for the smaller seed. i also use pennington finch seed from walmart. the key on straining out wbs is wire mesh strainers.

LIKE THIS



NOT LIKE THIS


generally the number one cause of failing with wild bird seed is overcooking it. when it turns into mush with mostly exploded grains after the pc cycle. do it again. don't even attempt to p2g if the grain looks like it has turned to mush. after the pc cycle i generally shake my jars when they are cool to the touch.

this is a good wbs prep method.

Fooman's Grain Tek

i add a dash of pickling lime to the boiled water bath. this helps get the gunk off the grain.


Edited by eatyualive (07/19/15 07:29 PM)

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