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SpinScratch
Distrusted Cultivator


Registered: 07/26/13
Posts: 834
Last seen: 7 months, 17 days
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Re: P2G(Pf Jar to Grain Transfer) [Re: eatyualive]
#20449954 - 08/20/14 07:00 PM (10 years, 4 months ago) |
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eatyualive said:ill also take clone tissue blend it in a blendar attachment to a pf jar, suck it into a syringe and inject it into a pf jar. ill generally do 1 syringe pr jar or two jars and then i have a cloned pf jar in less than a week.
wait... What? Let me get this straight. You put a mushroom in the blender with water and suck it into a syringe, and then inoculate a PF Jar with the entire syringe? I would think that would be loaded with contaminants. But why not skip a step and just use it to inoculate the grain jar?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: P2G(Pf Jar to Grain Transfer) [Re: SpinScratch]
#20450034 - 08/20/14 07:13 PM (10 years, 4 months ago) |
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Quote:
SpinScratch said:
Quote:
eatyualive said:ill also take clone tissue blend it in a blendar attachment to a pf jar, suck it into a syringe and inject it into a pf jar. ill generally do 1 syringe pr jar or two jars and then i have a cloned pf jar in less than a week.
wait... What? Let me get this straight. You put a mushroom in the blender with water and suck it into a syringe, and then inoculate a PF Jar with the entire syringe? I would think that would be loaded with contaminants. But why not skip a step and just use it to inoculate the grain jar?
no, i cut inner stem tissue from inside the stem, place it in the blender assembly then blend it. i then suck it up into a syringe and inject it into pf jars. it is done in a in a clean environment and all tools are sterilized before work is done. i use two separate knives one to cut the outside of the stem open, then the next to cut the tissue from the inside. a bernzomatic torch is used to sterilize all utensils. its the same thing if you cut a piece of stem tissue and place it on an agar plate. the only difference is that im placing it in sterile water, blending it up and then sucking it into a sterile syringe for liquid inoculation.
its called 9er tek. its one of the oldest and easiest liquid cloning techniques out there.
i only use the cloning part. it works so easily and the speed kills agar.
Edited by eatyualive (08/20/14 07:24 PM)
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Dilated
KB Cubensis Fanaticus



Registered: 03/28/14
Posts: 2,347
Loc: The Ether
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Re: P2G(Pf Jar to Grain Transfer) [Re: eatyualive]
#20450589 - 08/20/14 08:28 PM (10 years, 4 months ago) |
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Quote:
eatyualive said:
Quote:
SpinScratch said:
Quote:
eatyualive said:ill also take clone tissue blend it in a blendar attachment to a pf jar, suck it into a syringe and inject it into a pf jar. ill generally do 1 syringe pr jar or two jars and then i have a cloned pf jar in less than a week.
wait... What? Let me get this straight. You put a mushroom in the blender with water and suck it into a syringe, and then inoculate a PF Jar with the entire syringe? I would think that would be loaded with contaminants. But why not skip a step and just use it to inoculate the grain jar?
no, i cut inner stem tissue from inside the stem, place it in the blender assembly then blend it. i then suck it up into a syringe and inject it into pf jars. it is done in a in a clean environment and all tools are sterilized before work is done. i use two separate knives one to cut the outside of the stem open, then the next to cut the tissue from the inside. a bernzomatic torch is used to sterilize all utensils. its the same thing if you cut a piece of stem tissue and place it on an agar plate. the only difference is that im placing it in sterile water, blending it up and then sucking it into a sterile syringe for liquid inoculation.
its called 9er tek. its one of the oldest and easiest liquid cloning techniques out there.
i only use the cloning part. it works so easily and the speed kills agar.
Just a suggestion but you would only need to use one scalpel if you use your fingers to tear the stem apart to get at the tissue. Also if you cut the stem you maybe forcing contams into the mushroom stem.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 6 months, 13 days
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Re: P2G(Pf Jar to Grain Transfer) [Re: Dilated]
#20450848 - 08/20/14 09:00 PM (10 years, 4 months ago) |
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tombosley8 said: so i have some cakes (with sfd'd lids) and one is fully colonised and a couple days into consolidation. i have a few right behind it just about colonised. Can i use the cake without consolidation? Do you think a fork could be wrapped in foil and pc'd without needing alcohol wipe? wiping with alcohol scares me as this leads to failure with a syringe.
sooo.... ? sorry to be impatient but i'd like to do this asap
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: P2G(Pf Jar to Grain Transfer) [Re: tombosley8]
#20451059 - 08/20/14 09:35 PM (10 years, 4 months ago) |
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Quote:
tombosley8 said:
Quote:
tombosley8 said: so i have some cakes (with sfd'd lids) and one is fully colonised and a couple days into consolidation. i have a few right behind it just about colonised. Can i use the cake without consolidation? Do you think a fork could be wrapped in foil and pc'd without needing alcohol wipe? wiping with alcohol scares me as this leads to failure with a syringe.
sooo.... ? sorry to be impatient but i'd like to do this asap
ive used the method a thousand times above with no issues. try it. you can pc a fork it won't hurt. but isn't necessary. you need to be as sterile as possible with a spore syringe. with pg2 its healthy mycelia. it grows fast. if you colonize fast, you reduce the room for contams. ill keep osting hundreds of examples of this working perfectly if your in denial.
Edited by eatyualive (08/20/14 09:40 PM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: P2G(Pf Jar to Grain Transfer) [Re: Dilated]
#20451095 - 08/20/14 09:42 PM (10 years, 4 months ago) |
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Dilated said:
Quote:
eatyualive said:
Quote:
SpinScratch said:
Quote:
eatyualive said:ill also take clone tissue blend it in a blendar attachment to a pf jar, suck it into a syringe and inject it into a pf jar. ill generally do 1 syringe pr jar or two jars and then i have a cloned pf jar in less than a week.
wait... What? Let me get this straight. You put a mushroom in the blender with water and suck it into a syringe, and then inoculate a PF Jar with the entire syringe? I would think that would be loaded with contaminants. But why not skip a step and just use it to inoculate the grain jar?
no, i cut inner stem tissue from inside the stem, place it in the blender assembly then blend it. i then suck it up into a syringe and inject it into pf jars. it is done in a in a clean environment and all tools are sterilized before work is done. i use two separate knives one to cut the outside of the stem open, then the next to cut the tissue from the inside. a bernzomatic torch is used to sterilize all utensils. its the same thing if you cut a piece of stem tissue and place it on an agar plate. the only difference is that im placing it in sterile water, blending it up and then sucking it into a sterile syringe for liquid inoculation.
its called 9er tek. its one of the oldest and easiest liquid cloning techniques out there.
i only use the cloning part. it works so easily and the speed kills agar.
Just a suggestion but you would only need to use one scalpel if you use your fingers to tear the stem apart to get at the tissue. Also if you cut the stem you maybe forcing contams into the mushroom stem.
one is for the outer cut, one for inner. this isn't necessary i just do it this way. i have two its easy to pc them. i want no down time from the time i cut the stem tissue to placing it into the jar for blending. and i don't waste any time on the cut flame sterilizing anything. as soon as the stem is openeed, i cut very fast and drop the slice into the jar. next time i do it ill try and take pictures during the process. its very difficult without help to illustrate this live. ill try.
Edited by eatyualive (08/20/14 09:44 PM)
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 6 months, 13 days
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Re: P2G(Pf Jar to Grain Transfer) [Re: eatyualive]
#20451551 - 08/20/14 11:06 PM (10 years, 4 months ago) |
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do you recommend this over the liquid cake inoculation (slurry tek)? seems like the slurry tek would be better/easier? I have to or am choosing to do this over the slurry tek because i don't want to cut the cake in half even in front of my hood as i think i may contaminate it. but what you said about speed of colonization makes me think that maybe with my lack of confidence and possibly slight erorr i may still be ok? i ended pcing the fork because i'm so over the top with my sterile procedure but that's the only way i've been able to succeed. Didn't read your post until after. I believe you and i think i'm going to try them both out just wondering which you think i should try first? i have a few days until the other cakes are colonized so i have one cake to work with for now. i have tap water for LI or maybe the slurry tek cooling in the pc now.
Edited by tombosley8 (08/20/14 11:40 PM)
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 6 months, 13 days
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Re: P2G(Pf Jar to Grain Transfer) [Re: tombosley8]
#20454523 - 08/21/14 03:34 PM (10 years, 4 months ago) |
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so i ended up doing both and should be posting my results soon and continuing when the cakes are all done. Thanks again for such a well written and highly productive method. but one more thing, the inside of the cake appeared uncolonised when i scraped it up with the fork. Is this normal or was the cake not actually colonized, or maybe i should have waited for consolidation?
Edited by tombosley8 (08/21/14 03:55 PM)
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: P2G(Pf Jar to Grain Transfer) [Re: tombosley8]
#20454608 - 08/21/14 03:59 PM (10 years, 4 months ago) |
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Quote:
tombosley8 said: maybe i should have waited for consolidation?
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 6 months, 13 days
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well fuk i asked before and it seemed that he wouldve answered that if it was that important. Was that really a waste of a good cake and all that grain?
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: P2G(Pf Jar to Grain Transfer) [Re: tombosley8]
#20454649 - 08/21/14 04:11 PM (10 years, 4 months ago) |
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I didn't read all the posts, so I apologize if I missed something.
Consolidation of cakes is for when you intend to fruit them as cakes.
Don't consolidate if you plan P2G or spawn to bulk.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: P2G(Pf Jar to Grain Transfer) [Re: SpitballJedi]
#20454655 - 08/21/14 04:12 PM (10 years, 4 months ago) |
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SpitballJedi said: I didn't read all the posts, so I apologize if I missed something.
Consolidation of cakes is for when you intend to fruit them as cakes.
Don't consolidate if you plan P2G or spawn to bulk.
Ok I stand corrected. What's the reasoning behind this?
Edited by wowimflabbergasted (08/21/14 04:32 PM)
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 6 months, 13 days
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yaaaaayyyyyyy
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: P2G(Pf Jar to Grain Transfer) [Re: SpitballJedi]
#20455206 - 08/21/14 06:08 PM (10 years, 4 months ago) |
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Quote:
SpitballJedi said: I didn't read all the posts, so I apologize if I missed something.
Consolidation of cakes is for when you intend to fruit them as cakes.
Don't consolidate if you plan P2G or spawn to bulk.
Yes this is exactly correct. This is purely for using them to transfer from pf jar to grain. i don't leave them in the fridge intending to fruit directly off the cake. although i usually wait 3 days after full colonization and either p2g them or place them in the fridge. there have been several times that i have forgotten about the jars for a week and then stuck them in the fridge.
Edited by eatyualive (08/21/14 08:32 PM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: P2G(Pf Jar to Grain Transfer) [Re: tombosley8]
#20455218 - 08/21/14 06:11 PM (10 years, 4 months ago) |
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Quote:
tombosley8 said: do you recommend this over the liquid cake inoculation (slurry tek)? seems like the slurry tek would be better/easier? I have to or am choosing to do this over the slurry tek because i don't want to cut the cake in half even in front of my hood as i think i may contaminate it. but what you said about speed of colonization makes me think that maybe with my lack of confidence and possibly slight erorr i may still be ok? i ended pcing the fork because i'm so over the top with my sterile procedure but that's the only way i've been able to succeed. Didn't read your post until after. I believe you and i think i'm going to try them both out just wondering which you think i should try first? i have a few days until the other cakes are colonized so i have one cake to work with for now. i have tap water for LI or maybe the slurry tek cooling in the pc now.
the slurry inoculation is really good if you need a giant amount of spawn like 100 jars using one pf cake. i don't even own that many jars. so to me, i feel that it would be wasting half of the cake if i only used it to do 10 quart jars. id rather use all the mycelia with a fork into 10 quarts. also the slurry is good if you need a ton of spawn and you have very little to work with.
also, this takes some practice. it did work for me the first few times i tried it. i tried it several times before perfecting it and i have alot of experience. so, be patient. pressure cooking the fork is great and the more anal you are about the clean procedure the better. you can never be too anal about clean work.
Edited by eatyualive (08/21/14 06:16 PM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: P2G(Pf Jar to Grain Transfer) [Re: tombosley8]
#20455223 - 08/21/14 06:13 PM (10 years, 4 months ago) |
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tombosley8 said: so i ended up doing both and should be posting my results soon and continuing when the cakes are all done. Thanks again for such a well written and highly productive method. but one more thing, the inside of the cake appeared uncolonised when i scraped it up with the fork. Is this normal or was the cake not actually colonized, or maybe i should have waited for consolidation?
great id love to see the results. ive seen it on spawn bags/quarts/ popcorn grain jars and all types of different grains with success. but you have to remember, you only need a tiny little bit per jar. you don't want to over pour. its almost like one slimy drop or two slimy drops of slurry.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 6 months, 13 days
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Re: P2G(Pf Jar to Grain Transfer) [Re: tombosley8]
#20456058 - 08/21/14 08:27 PM (10 years, 4 months ago) |
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o ok i thought it was more like a lc where around 2 ccs would be good. I sucked some up into some syringes and have them in the fridge until i have grain ready in a couple days so i'll do just a couple drops per and i'll hopefully have another cake ready to try it on again. I think i mixed the cake with too much water (I used a qt half full)and only used half the cake.... This time i'll probably use the whole cake or just a pint jar... thanks for all your help again i can't wait to perfect this as well.
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Edited by tombosley8 (08/21/14 08:28 PM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: P2G(Pf Jar to Grain Transfer) [Re: tombosley8]
#20456106 - 08/21/14 08:36 PM (10 years, 4 months ago) |
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tombosley8 said: o ok i thought it was more like a lc where around 2 ccs would be good. I sucked some up into some syringes and have them in the fridge until i have grain ready in a couple days so i'll do just a couple drops per and i'll hopefully have another cake ready to try it on again. I think i mixed the cake with too much water (I used a qt half full)and only used half the cake.... This time i'll probably use the whole cake or just a pint jar... thanks for all your help again i can't wait to perfect this as well.
i hope this works. although im not sure that it will. i generally wait till the grain is ready before i blend up the cake. then blend it up and pour it into the grain after i just blended it up. id say your adding some room for issues by having it sit in a syringe. Read through the entire Tv Casualty's Slurry in a Hurry Tek.
I hope you have luck. the 2cc's may work. its not an exact science unless you make the lids like TV did. He has a nozzle attachment to the top of his lid. I was lazy and just poured it from the blender jar right into quart jars. His method is probably cleaner or more measured. But its always best to prep everything before doing this and then pour or inject the jars immediately with a slurry. If it does work, then you may have figured something new out. I store the colonized pf jars in the fridge. when i have my grain prepped and setup all my clean area and tools, ill then take out the pf jar from the fridge. then blend up and pour into the already prepped jars. i hope what im saying isn't confusing you. if it is please feel free to keep asking questions ill try and help any way i can.
Edited by eatyualive (08/21/14 08:44 PM)
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
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Re: P2G(Pf Jar to Grain Transfer) [Re: eatyualive]
#20456231 - 08/21/14 08:57 PM (10 years, 4 months ago) |
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no completely straight forward and with that advice i will probably not use the syringes afterall. It's worth the better chance of success to wait til i have another cake colonized. we'll probably have to wait til then for me to show results as i'm not so confident with the first attempt and may dump most of them but that's the best way to learn sometimes.... failure.... also i forgot to add that my cake was a bit mushy when trying to break it up so i was left with some large chunks. Should i adjust my cake recipe to a bit dryer? Maybe i'll see some success from the pf 2 g jars. I only ended up getting some into 4 jars from half a cake because of the clumped cake issue but i think that's about what i wanted right?
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Edited by tombosley8 (08/21/14 09:04 PM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: P2G(Pf Jar to Grain Transfer) [Re: tombosley8]
#20457847 - 08/22/14 05:52 AM (10 years, 4 months ago) |
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tombosley8 said: no completely straight forward and with that advice i will probably not use the syringes afterall. It's worth the better chance of success to wait til i have another cake colonized. we'll probably have to wait til then for me to show results as i'm not so confident with the first attempt and may dump most of them but that's the best way to learn sometimes.... failure.... also i forgot to add that my cake was a bit mushy when trying to break it up so i was left with some large chunks. Should i adjust my cake recipe to a bit dryer? Maybe i'll see some success from the pf 2 g jars. I only ended up getting some into 4 jars from half a cake because of the clumped cake issue but i think that's about what i wanted right?
don't throw them out. wait to see if they colonize. did you do the slurry? if so they should done in about 3 to 4 days. if you did the p2g it will take about 5-7 days. i generally shake on day 3. for the sake of experiment, id probably shoot that syringe into a few jars just to see what happens. if it contams toss it, if not you may have figured something out. i do store blended stem tissue in distilled water in syringes with no issues in the fridge. im not so sure about a blended up liquid pf cake that has chunks of brown rice flour in it. colonized or not. but i its worth a try everything is a few times.
Edited by eatyualive (08/22/14 05:57 AM)
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