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OfflineLittlePygmyMan
Space Cadet

Registered: 03/02/03
Posts: 215
Loc: Calimifornias
Last seen: 13 years, 2 months
Interesting Syringe Expierience.
    #2040563 - 10/24/03 07:43 PM (14 years, 29 days ago)

So... This one guy I know who grows shrooms gos to check his incubator. Which has no drain on the bottom and is made of plastic. Anyways... he opens it up, and it his suprise one of his bigguns had dropped it's spores... Which in turn have formed this cluster/puddle in the water thats gathered on the bottom of the incubator. All the water used in the humidifier is distilled and the incubator is cleaned regularly, so he runs to the fridge and grabs a syringe. Heats the needles, let's it cool... Sticks it in the puddle of spores and sucks em all up. Made the darkest syringe I have ever seen  :grin: He then filled up the extra 2 ccs with distilled water and capped it. I will let yu guys know what happned after inoculation.


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-LittlePygmyMan


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InvisibleGonzoCool

Registered: 10/01/03
Posts: 726
Loc: Flag
Re: Interesting Syringe Expierience. [Re: LittlePygmyMan]
    #2040567 - 10/24/03 07:46 PM (14 years, 29 days ago)

whoa, smooth


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Invisibledannycorona82
Stranger
Registered: 03/05/03
Posts: 326
Re: Interesting Syringe Expierience. [Re: GonzoCool]
    #2041051 - 10/24/03 11:33 PM (14 years, 29 days ago)

You might get contams...


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InvisibleSemilanceata
No god, no boss

Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Re: Interesting Syringe Expierience. [Re: LittlePygmyMan]
    #2041415 - 10/25/03 03:25 AM (14 years, 29 days ago)

Incubator?


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Sr_Setahongo


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OfflineLittlePygmyMan
Space Cadet

Registered: 03/02/03
Posts: 215
Loc: Calimifornias
Last seen: 13 years, 2 months
Re: Interesting Syringe Expierience. [Re: Semilanceata]
    #2041727 - 10/25/03 01:37 PM (14 years, 29 days ago)

Err, Grow Chamber, sorry.


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-LittlePygmyMan


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InvisibleDiploidM
Cuban

Folding@home Statistics
Registered: 01/09/03
Posts: 19,274
Loc: Rabbit Hole
Trusted Cultivator
Re: Interesting Syringe Expierience. [Re: LittlePygmyMan]
    #2042204 - 10/25/03 04:53 PM (14 years, 28 days ago)

That syringe is almost certainly contaminated. Remember, there can't be so much as a single bacteria in it. The chances that the water at the bottom of even a very clean chamber is sterile, is very small.

-Diploid


--------------------
Republican Values:

1) You can't get married to your spouse who is the same sex as you.
2) You can't have an abortion no matter how much you don't want a child.
3) You can't have a certain plant in your possession or you'll get locked up with a rapist and a murderer.

4) We need a smaller, less-intrusive government.


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Offlinebiglo
Shroomery BabySitter
 User Gallery

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Registered: 11/22/02
Posts: 603
Loc: US of A
Last seen: 2 years, 3 months
Re: Interesting Syringe Expierience. [Re: Diploid]
    #2042221 - 10/25/03 05:00 PM (14 years, 28 days ago)

If there was an overabundance of spores in the solution, would the mushroom spores germinate and outcompete the bacteria though? I doubt that there is never a single bacteria microbe in a majority of grow setups and syringes, but I agree, giving bacteria a foothold eitherway is a bad idea in any situation.


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OfflineTrippinSpinners
Mr. Nice Guy

Registered: 10/14/03
Posts: 305
Last seen: 12 years, 1 month
Re: Interesting Syringe Expierience. [Re: biglo]
    #2042232 - 10/25/03 05:04 PM (14 years, 28 days ago)

I would try it.... try using wbs... not much to loose....


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When I use the name "I", I'm talking about my friend of a friend named "Ivan".

--Mr. Nice Guy


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InvisibleDiploidM
Cuban

Folding@home Statistics
Registered: 01/09/03
Posts: 19,274
Loc: Rabbit Hole
Trusted Cultivator
Re: Interesting Syringe Expierience. [Re: biglo]
    #2042243 - 10/25/03 05:09 PM (14 years, 28 days ago)

Quote:

If there was an overabundance of spores in the solution, would the mushroom spores germinate and outcompete the bacteria though?




Nope.

Bacteria grow at an exponential rate. Even a single bacteria will far outpace spores. A single bacteria incubated at 85F in a nutritious environment and dividing at a rate of once every hour will become over 16 million bacteria in a day and 280 TRILLION in two days. Properly produced syringes are 100% TOTALLY sterile. All commercially produced syringes are guaranteed so.

-Diploid


--------------------
Republican Values:

1) You can't get married to your spouse who is the same sex as you.
2) You can't have an abortion no matter how much you don't want a child.
3) You can't have a certain plant in your possession or you'll get locked up with a rapist and a murderer.

4) We need a smaller, less-intrusive government.


Post Extras: Print Post  Remind Me! Notify Moderator
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