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Offlinestoner2002
Stoner

Registered: 11/15/02
Posts: 183
Last seen: 13 years, 2 months
How long should I PC my spoon?
    #2039426 - 10/24/03 02:39 PM (13 years, 5 months ago)

How long do you guys sterilize things like syringes and tools for doing g2g transfers? I put my tools in a quart jar full of water and PCed for 30 min so far. Is that sufficient? I dont have to go an hour like I do with the seed do I?

Also, when people say 100% colonized do they mean 100%? Like if theres the a few kernels of millet that arent colonizing am I supposed to wait? Theyve been stalled for like a week, its fucking frustrating. They're going to be used for g2g transfers, not fruiting, should I do them anyways?


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Invisibledaussaulit
Forgetful

Registered: 08/06/02
Posts: 2,894
Loc: Earth
Re: How long should I PC my spoon? [Re: stoner2002]
    #2039476 - 10/24/03 02:55 PM (13 years, 5 months ago)

I think PCing may be going overboard. Just put it in a bleach water solution


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InvisibleSemilanceata
No god, no boss

Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Re: How long should I PC my spoon? [Re: stoner2002]
    #2039605 - 10/24/03 03:20 PM (13 years, 5 months ago)

Wrap it in tin foil and boil it for 30 minutes, wrap it airtight so no water goes in (just if you were cooking papillote), or bake it at full power for 30 minutes or more. If you are sterilizing jars, you can put any metalware wrapped in tin foil with the jars, but you don?t need to use the PC just for metalware.

If you have a few kernels not colonized it?s not a big deal, specially if the kernels are kind of glued to the glass. If it?s a spot, you should wait. Did you shake the jars?


--------------------
Sr_Setahongo


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Offlinestoner2002
Stoner

Registered: 11/15/02
Posts: 183
Last seen: 13 years, 2 months
Re: How long should I PC my spoon? [Re: Semilanceata]
    #2039704 - 10/24/03 03:44 PM (13 years, 5 months ago)

I pced the spoons for like 45 minutes, oh well. :P

Yes semi, the kernels are just like individual kernels stuck to the glass, not big clumps. They're being stubborn. I contribute them stalling to my lack of sufficient air exchange. I messed with my polyfill, it was crammed way to tight.

Theyve got a couple days before its transfer time, hopefully they finish.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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