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Stranger


Registered: 08/06/14
Posts: 13
Last seen: 9 years, 9 months
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Hi all! PF tek beginner here
#20381567 - 08/06/14 06:50 AM (10 years, 5 months ago) |
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So... where do I start, well this isn't my very first time trying to grow, the first time took place a couple years ago and no sterilization was taken (no more explanation required) haha.
I decided to make an account and join the community in hopes to learn and grow. This being my 2nd grow or i guess you could say my first serious grow, I am weary I have screwed up again. This site hosts an abundance of information and sometimes its easy for a noob to screw up; whats original, whats experimental, whats left out and whats emphasized.
My grow that is 6 days in right now: I followed the PF tek to a T up in till sterilization. So here is what I did, while my jars were sitting ready in the PC. Took a shower,made sure to scrub real good to get dead skin cells off, after that I put on some newly laundered clothes and sprayed the shit out of the bathroom with lysol let it sit for 30 min and did it again and let it sit for another 30. I then put on another set of clothes grabbed my needle a lighter, rubbing alcohol (91%) and pc and proceeded in the bathroom. I could tell the lysol was thick in there, almost hard to breathe. I took the lid off of the Pc and took one jar out at a time, with the foil still on the jars I "sterilized" my needle (but not till it was red hot) I simply ran it under the lighter for a couple seconds. I did this because I didn't know it only took a couple seconds to cool down, I thought it took longer, therefore leaving the risk of a contaminant getting on the needle. I removed the foil stuck my needle in the hole... and what do you know, the holes were too small. What I did next could possibly contaminated my grow too. with some force I grabbed the needle with my left hand and my right on the syringe I forced the needle through. I pushed until I seen liquid hit the glass, removed the needle rubbed it with an alcohol laced rag and then flamed it every time per hole on the jar, with every jar. I put the foil back on the jars each time I finished one. Now realizing that i touched the needle for every hole/every jar.. I think I'm fucked... I have the Jars sitting in my closet at 72-75F 6 days later. no sign of mycelium yet
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Bumbaclotjohnson
Sequoia Farmer



Registered: 01/12/14
Posts: 1,067
Loc: USA
Last seen: 4 years, 9 months
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Re: Hi all! PF tek beginner here [Re: Switchback]
#20381572 - 08/06/14 06:53 AM (10 years, 5 months ago) |
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-------------------- FREE PRINTS
I lie a lot on the internet.
trade list
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BurberrySpores
72 zones



Registered: 08/05/14
Posts: 1,013
Last seen: 9 months, 14 days
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omg @ your clothes changes, and hour waiting for your air to settle.
you should just get a Still Air Box.
-------------------- I could go crazy... and no one would notice
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Quexl


Registered: 12/17/13
Posts: 1,443
Loc:
Last seen: 9 years, 10 months
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Quote:
BurberrySpores said: you should just get a Still Air Box.
And don't worry about fumigating your bathroom, just use a room without any breezes and a SAB like Bur said. Bathrooms are terrible for sterility.
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Switchback
Stranger


Registered: 08/06/14
Posts: 13
Last seen: 9 years, 9 months
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Thanks for the link. As for the clothes changes and the waiting I don't see a problem with it. As for a still air box I have one I think I'll use it next time. In preparation of the SAB do you guys disinfect inside of the box let stand for a bit then introduce your jars inside?
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BurberrySpores
72 zones



Registered: 08/05/14
Posts: 1,013
Last seen: 9 months, 14 days
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Re: Hi all! PF tek beginner here [Re: Switchback]
#20381854 - 08/06/14 09:23 AM (10 years, 5 months ago) |
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Quote:
Switchback said: Thanks for the link. As for the clothes changes and the waiting I don't see a problem with it. As for a still air box I have one I think I'll use it next time. In preparation of the SAB do you guys disinfect inside of the box let stand for a bit then introduce your jars inside?
its that its a problem 
you just need to do it twice .
just shower, dress, and clean out your SAB.
wipde down your jars before you put them in you SAB
wipe them again inside your SAB.
you can spray some lysol or bleach water.
let the air settle, and get to work.
wear gloves, flame tip your needle or scalpel. etc
-------------------- I could go crazy... and no one would notice
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Skinty
TOP SECRET


Registered: 07/04/13
Posts: 1,150
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Re: Hi all! PF tek beginner here [Re: Switchback]
#20381864 - 08/06/14 09:26 AM (10 years, 5 months ago) |
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As Quexl indicated your bathroom is likely the dirtiest room in a house no matter what lysol spraying regime you do
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Switchback
Stranger


Registered: 08/06/14
Posts: 13
Last seen: 9 years, 9 months
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Re: Hi all! PF tek beginner here [Re: Skinty]
#20395094 - 08/09/14 10:09 AM (10 years, 5 months ago) |
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Hey guys 9 days in, mycelium has taken off! All are looking good, no discolorations or anything, just some FAT mycelium inoculation points. I have 6 jars going right now I would have ten, but little overkill on the inoculation. As soon as these jars are ready to be birthed I plan on taking 5 of these and finishing them off as cakes. As for the other one I was thinking of breaking it up and splitting it up in rye jars. or possibly breaking it up and putting it in a monotub. Thoughts?
Thanks!
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BurberrySpores
72 zones



Registered: 08/05/14
Posts: 1,013
Last seen: 9 months, 14 days
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Re: Hi all! PF tek beginner here [Re: Switchback]
#20395180 - 08/09/14 10:40 AM (10 years, 5 months ago) |
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Pics or it didn't happen
-------------------- I could go crazy... and no one would notice
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PrincessPeach


Registered: 07/16/14
Posts: 121
Last seen: 10 years, 3 months
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So like I did 12 jars my first run. I was clean about it, but I didn't sterilize the needle between holes or even turn my a/c off or anything like that. All 12 of my jars made it. I don't believe it's necessary to go sterile crazy, just be smart about. Wash your hands, any thing your working with, any counter your using. Don't touch stuff if you don't need to etc. Also I only boiled my jars for an hour. I think extremes like this are only necessary when you know you have an existing issue in your environment with contamination.
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Jman420
MagicJ



Registered: 12/23/12
Posts: 866
Loc: On a Cloud
Last seen: 4 years, 8 months
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I think everyone should start out with the monotub tek.  Much better results, always.
Gl
-------------------- --My Everything You Need To Know Tek--
Disregard the haters and "know it alls", it is everything you need to know TO GET STARTED.
If you want to become an expert, you'll need to do a lot more research.
-----------------------------------------------------------------------
Open Your Mind To A New Future
-----------------------------------------------------------------------
    
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Switchback
Stranger


Registered: 08/06/14
Posts: 13
Last seen: 9 years, 9 months
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Re: Hi all! PF tek beginner here [Re: Jman420]
#20469424 - 08/24/14 11:06 AM (10 years, 4 months ago) |
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Hey guys, 24 days later and I would assume the Jars are fully colonized, oh yeah I lied I actually have 8 Jars I know I stated 6. Anyways the jars.. 4 are all white no contamination visible, I plan on giving them a few more days before they enter a FC, as for the other four they seem to be stalled. The mycelium is still colonizing but unbelievably slow. I ordered some supplies from outgrow and I got 2 free Filter discs thrown in, (Thanks!) so I took the lid off of one and placed a filter disk on top possibly to get some fresh air in there? I read its possible that they need a little more FAE than what they have been getting.
The 4 will go into a tote with perlite ect... and I have a aquarium air pump to be pumping constant fresh air into it, so no fanning will be necessary???
what would you guys recommend for fruiting temperature, I have read a lot and most is contradicting..
One more thing, I am not going to post pictures yet, I will when its all said and done
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Hi all! PF tek beginner here [Re: Switchback] 1
#20469442 - 08/24/14 11:10 AM (10 years, 4 months ago) |
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Quote:
The 4 will go into a tote with perlite ect... and I have a aquarium air pump to be pumping constant fresh air into it, so no fanning will be necessary???
never use an air pump. They DO NOT replace fanning at all.
you need to do some reading big time.(click the first link in my signature)
Fruit between 60-100F colonize between 60-78F(cubensis mycelium slows down at an internal temp of 82F which is reached by about 78F outside of the jars) room temperature is fine for all aspects of growing cubensis, if your room temperature is above 60F and below 80F then don't try to do anything special.
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Quexl


Registered: 12/17/13
Posts: 1,443
Loc:
Last seen: 9 years, 10 months
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Re: Hi all! PF tek beginner here [Re: Switchback]
#20469458 - 08/24/14 11:13 AM (10 years, 4 months ago) |
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:D
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beerdedguy
stunt cock
Registered: 08/10/14
Posts: 24
Last seen: 7 years, 7 months
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Re: Hi all! PF tek beginner here [Re: Jman420]
#20469463 - 08/24/14 11:15 AM (10 years, 4 months ago) |
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Quote:
Jman420 said: I think everyone should start out with the monotub tek.  Much better results, always.
Gl
That's what I did. I was going to just do the brf cakes in a sgfc but decided to do a monotub instead.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Hi all! PF tek beginner here [Re: bodhisatta] 1
#20469472 - 08/24/14 11:16 AM (10 years, 4 months ago) |
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91% alcohol doesn't work as good as 70% alcohol for sanitation. save your 91% for cleaning pipes.
The bathroom is dirty as fuck don't do any mycology work in a bathroom.
as soon as sterilization is done the foil comes off and stays off. you don't want the foil to stay on unless you want contamination to grow in the dry vermiculite layer.
lysol does shit for making a room clean, ditch lysol never buy it again unless you're cleaning your bathroom and even then lysol sucks compared to some ammonia and water.
build a SAB. You only need to spray the inside of a SAB with very diluted dish soap water. 1 drop soap to a quart of water. Still air box not sanitary or sterile air box. The still air is what keeps your work sterile, the inner surfaces of a SAB could be dirty as shit but the still air is what's protecting you. Don't spray lysol or alcohol inside of a SAB.
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LumpyNutz
Bad fisH <*))))<



Registered: 07/14/14
Posts: 712
Loc: Stuck in this dream
Last seen: 7 years, 5 months
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Quote:
PrincessPeach said: So like I did 12 jars my first run. I was clean about it, but I didn't sterilize the needle between holes or even turn my a/c off or anything like that. All 12 of my jars made it. I don't believe it's necessary to go sterile crazy, just be smart about. Wash your hands, any thing your working with, any counter your using. Don't touch stuff if you don't need to etc. Also I only boiled my jars for an hour. I think extremes like this are only necessary when you know you have an existing issue in your environment with contamination.
I use the same sterilization/sanitation technique and have very few issues with contam!
Also I: 1) WIPE NEEDLE WITH A PAPER TOWEL WITH RUBBING ALC ON IT! 2) Flame sterilize needle 3) Innoc 1 jar (all 4 holes) 4) ...Repeat steps 1-4
Is this the proper technique???
-------------------- This is uh... no offence... but you are a robot aren't you?
 
GET SHPONGLED
Edited by LumpyNutz (09/06/14 04:24 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Hi all! PF tek beginner here [Re: LumpyNutz] 1
#20469597 - 08/24/14 11:39 AM (10 years, 4 months ago) |
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no you wipe then flame. alcohol = sanitary flame = sterile
don't work backwards
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Hi all! PF tek beginner here [Re: bodhisatta]
#20469630 - 08/24/14 11:45 AM (10 years, 4 months ago) |
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Quote:
RogerRabbit said: Wiping with alcohol after flame sterilizing is like wiping shit on your face after taking a shower. RR
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Bumbaclotjohnson
Sequoia Farmer



Registered: 01/12/14
Posts: 1,067
Loc: USA
Last seen: 4 years, 9 months
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Re: Hi all! PF tek beginner here [Re: bodhisatta]
#20472187 - 08/24/14 09:28 PM (10 years, 4 months ago) |
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Quote:
bodhisatta said:
Quote:
RogerRabbit said: Wiping with alcohol after flame sterilizing is like wiping shit on your face after taking a shower. RR
This is by far my favorite rr quote I think about it randomly when inoculating jars.
-------------------- FREE PRINTS
I lie a lot on the internet.
trade list
Edited by Bumbaclotjohnson (08/24/14 09:29 PM)
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LumpyNutz
Bad fisH <*))))<



Registered: 07/14/14
Posts: 712
Loc: Stuck in this dream
Last seen: 7 years, 5 months
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Re: Hi all! PF tek beginner here [Re: bodhisatta]
#20474067 - 08/25/14 10:03 AM (10 years, 4 months ago) |
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Quote:
bodhisatta said: no you wipe then flame. alcohol = sanitary flame = sterile
don't work backwards
Shit I will change my technique then, thanks for clarifying man . I was thinking on my last 'noc I was doing it backwards, but was too lazy to look it up.. I tend to be a little dyslectic
-------------------- This is uh... no offence... but you are a robot aren't you?
 
GET SHPONGLED
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Switchback
Stranger


Registered: 08/06/14
Posts: 13
Last seen: 9 years, 9 months
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Re: Hi all! PF tek beginner here [Re: LumpyNutz]
#20474308 - 08/25/14 11:28 AM (10 years, 4 months ago) |
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ok no airpump, as for the Jars I don't see any contamination so I call my sterilization a win for a dirty fuckin bathroom.
Also Ill post some pics

My jars, SAB, air pump idea... and a whole bunch of straw waiting
which reminds me, I'm planning on using the rye in picture 1 for a straw basket/monotub, I do not plan on using nothing else except straw (input please) Also I do not have any hydrated lime, I have heard you can use bleach as a substitute (input)
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Switchback
Stranger


Registered: 08/06/14
Posts: 13
Last seen: 9 years, 9 months
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Re: Hi all! PF tek beginner here [Re: Switchback]
#20474312 - 08/25/14 11:29 AM (10 years, 4 months ago) |
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One more thing, I appreciate the input from everyone so far, Thank you for the help!
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Bumbaclotjohnson
Sequoia Farmer



Registered: 01/12/14
Posts: 1,067
Loc: USA
Last seen: 4 years, 9 months
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Re: Hi all! PF tek beginner here [Re: LumpyNutz]
#20474484 - 08/25/14 12:23 PM (10 years, 4 months ago) |
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Don't even bother wiping just flame
-------------------- FREE PRINTS
I lie a lot on the internet.
trade list
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Switchback
Stranger


Registered: 08/06/14
Posts: 13
Last seen: 9 years, 9 months
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As the day goes on my head starts running off ideas and I get my self so worked up about how I'm going to proceed after my jars are fully colonized, I know I am going to use most of them as they are (cakes) but I'm thinking I want to use one for some more rye Jars, and If possible I want to break one up and use in a monotub. I have been reading substrates and I don't think I have my head entirely wrapped around all of the possible substrates and why they are used. If someone could help me out\ with my monotub I would appreciate it. Right now I have cut up straw, vermiculite, BRF, Rye berries, Gypsum and some unknown birdseed out in the garage. Also some horse poo is a possibility from a friend, and unlimited worm casting from a business. Dealing with these circumstances what would you do for a monotub substrate.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Hi all! PF tek beginner here [Re: Switchback]
#20480112 - 08/26/14 04:31 PM (10 years, 4 months ago) |
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use coir vermiculite and gypsum as your first monotub substrate. It's the hardest to fuck up, it's the easiest to prepare, and it gives just as good results.
also Im not sure if your gloves are attached to your SAB. the gloves should not be attached.
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Switchback
Stranger


Registered: 08/06/14
Posts: 13
Last seen: 9 years, 9 months
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Re: Hi all! PF tek beginner here [Re: bodhisatta]
#20526239 - 09/05/14 10:31 PM (10 years, 4 months ago) |
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Hey guys, been awhile since I posted, give ya an update, cakes have been in fc for 3 days 2 cakes have excelled and have been picked, and are now in the dunking process again. I have a question, on a cake I had a mushroom that was significantly bigger than the others and the veil broke about 10hrs before any others were even close. From reading i was told pick at 70% veil break, so I did. But while it took 10hrs for the others to be ready what do you do with such a small amount of mushies? throw it in the dehydrator cause that's what i did. Also for the small guys (possibly aborts) I picked them too since I didn't see any change in size, the heads did not smush nor smell funny. Was I wrong to pick them early?
waiting till tomorrow morning I plan on having another cake ready to go tomorrow
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Sanguin3
Optimist

Registered: 10/19/13
Posts: 2,273
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Re: Hi all! PF tek beginner here *DELETED* [Re: Switchback]
#20526762 - 09/06/14 12:33 AM (10 years, 4 months ago) |
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Post deleted by Sanguin3
Reason for deletion: .
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Switchback
Stranger


Registered: 08/06/14
Posts: 13
Last seen: 9 years, 9 months
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Re: Hi all! PF tek beginner here [Re: Sanguin3]
#20529283 - 09/06/14 04:21 PM (10 years, 4 months ago) |
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Awesome thanks!
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Switchback
Stranger


Registered: 08/06/14
Posts: 13
Last seen: 9 years, 9 months
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Re: Hi all! PF tek beginner here [Re: Switchback]
#20588911 - 09/19/14 02:48 PM (10 years, 3 months ago) |
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Just to give everyone an update, had a pretty good run got 30.1g from 8 cakes! I took the cakes blended them up and found a spot outside for them. I took 3 cakes broke one up and put it in a coir Verm mini mono, colonizing well. I then I took 2 blended them up and put them in another one. I also have had 2 rye jars going during this and one just now has started showing growth. That took a long f**king time.... I'll be probably getting some more cakes going just as a plan B
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PsyDragon
journeyman


Registered: 10/21/05
Posts: 627
Loc: state of perpetual transi...
Last seen: 5 years, 7 months
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Re: Hi all! PF tek beginner here [Re: Switchback]
#20588987 - 09/19/14 03:03 PM (10 years, 3 months ago) |
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I'm glad this has been successful for you. I hope you're having fun!
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tool8te
Stranger


Registered: 09/09/14
Posts: 52
Last seen: 9 years, 3 months
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Re: Hi all! PF tek beginner here [Re: PsyDragon]
#20590314 - 09/19/14 08:46 PM (10 years, 3 months ago) |
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So my wbs is at 10% and I tried shaking them because they were growing at the bottom... well I shook it and none of the Colonized grain at the bottom moved... what should I do?
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Sanguin3
Optimist

Registered: 10/19/13
Posts: 2,273
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Re: Hi all! PF tek beginner here *DELETED* [Re: tool8te]
#20590330 - 09/19/14 08:51 PM (10 years, 3 months ago) |
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Post deleted by Sanguin3
Reason for deletion: .
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tool8te
Stranger


Registered: 09/09/14
Posts: 52
Last seen: 9 years, 3 months
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Re: Hi all! PF tek beginner here *DELETED* [Re: Sanguin3]
#20590341 - 09/19/14 08:53 PM (10 years, 3 months ago) |
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Post deleted by tool8teReason for deletion: *
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Sanguin3
Optimist

Registered: 10/19/13
Posts: 2,273
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Re: Hi all! PF tek beginner here *DELETED* [Re: tool8te]
#20590352 - 09/19/14 08:55 PM (10 years, 3 months ago) |
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Post deleted by Sanguin3
Reason for deletion: .
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Switchback
Stranger


Registered: 08/06/14
Posts: 13
Last seen: 9 years, 9 months
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Re: Hi all! PF tek beginner here [Re: Sanguin3]
#20591992 - 09/20/14 08:57 AM (10 years, 3 months ago) |
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having a blast with this hobby!, might try getting some lc's going
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Re: Hi all! PF tek beginner here [Re: Switchback]
#20592033 - 09/20/14 09:15 AM (10 years, 3 months ago) |
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Quote:
Switchback said: having a blast with this hobby!, might try getting some lc's going
Don't bother....LCs are garbage.....get into agar.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Hi all! PF tek beginner here [Re: Switchback]
#20592037 - 09/20/14 09:16 AM (10 years, 3 months ago) |
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Quote:
Switchback said: having a blast with this hobby!, might try getting some lc's going
that's an easy way not to have a blast. adding another step into inoculation slows you down and adds a vector for contamination. LC are not faster as you may think since you're adding a step even though if you can manage to get a clean LC the jars may colonize faster if you would have just inoculated them in the first place they would have been further ahead.
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