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OfflineSwitchback
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Hi all! PF tek beginner here
    #20381567 - 08/06/14 06:50 AM (10 years, 5 months ago)

So... where do I start, well this isn't my very first time trying to grow, the first time took place a couple years ago and no sterilization was taken (no more explanation required) haha.

I decided to make an account and join the community in hopes to learn and grow. This being my 2nd grow or i guess you could say my first serious grow, I am weary I have screwed up again. This site hosts an abundance of information and sometimes its easy for a noob to screw up; whats original, whats experimental, whats left out and whats emphasized.

My grow that is 6 days in right now: I followed the PF tek to a T up in till sterilization. So here is what I did, while my jars were sitting ready in the PC. Took a shower,made sure to scrub real good to get dead skin cells off, after that I put on some newly laundered clothes and sprayed the shit out of the bathroom with lysol let it sit for 30 min and did it again and let it sit for another 30. I then put on another set of clothes grabbed my needle a lighter, rubbing alcohol (91%) and pc and proceeded in the bathroom. I could tell the lysol was thick in there, almost hard to breathe. I took the lid off of the Pc and took one jar out at a time, with the foil still on the jars I "sterilized" my needle (but not till it was red hot) I simply ran it under the lighter for a couple seconds. I did this because I didn't know it only took a couple seconds to cool down, I thought it took longer, therefore leaving the risk of a contaminant getting on the needle. I removed the foil stuck my needle in the hole... and what do you know, the holes were too small. What I did next could possibly contaminated my grow too. with some force I grabbed the needle with my left hand and my right on the syringe I forced the needle through. I pushed until I seen liquid hit the glass, removed the needle rubbed it with an alcohol laced rag and then flamed it every time per hole on the jar, with every jar. I put the foil back on the jars each time I finished one. Now realizing that i touched the needle for every hole/every jar.. I think I'm fucked... I have the Jars sitting in my closet at 72-75F 6 days later. no sign of mycelium yet

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OfflineBumbaclotjohnson
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Re: Hi all! PF tek beginner here [Re: Switchback]
    #20381572 - 08/06/14 06:53 AM (10 years, 5 months ago)



--------------------
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OfflineBurberrySpores
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Re: Hi all! PF tek beginner here [Re: Bumbaclotjohnson]
    #20381713 - 08/06/14 08:13 AM (10 years, 5 months ago)

omg @ your clothes changes, and hour waiting for your air to settle.

you should just get a Still Air Box.


--------------------
I could go crazy... and no one would notice :seeya:

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OfflineQuexl
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Re: Hi all! PF tek beginner here [Re: BurberrySpores]
    #20381783 - 08/06/14 08:53 AM (10 years, 5 months ago)

Quote:

BurberrySpores said:
you should just get a Still Air Box.




And don't worry about fumigating your bathroom, just use a room without any breezes and a SAB like Bur said. Bathrooms are terrible for sterility.


--------------------
grain spawn bacteria info

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OfflineSwitchback
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Re: Hi all! PF tek beginner here [Re: BurberrySpores]
    #20381838 - 08/06/14 09:15 AM (10 years, 5 months ago)

Thanks for the link. As for the clothes changes and the waiting I don't see a problem with it. As for a still air box I have one I think I'll use it next time. In preparation of the SAB do you guys disinfect inside of the box let stand for a bit then introduce your jars inside?

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OfflineBurberrySpores
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Re: Hi all! PF tek beginner here [Re: Switchback]
    #20381854 - 08/06/14 09:23 AM (10 years, 5 months ago)

Quote:

Switchback said:
Thanks for the link. As for the clothes changes and the waiting I don't see a problem with it. As for a still air box I have one I think I'll use it next time. In preparation of the SAB do you guys disinfect inside of the box let stand for a bit then introduce your jars inside?




its that its a problem :lol:

you just need to do it twice :smile:.

just shower, dress, and clean out your SAB.

wipde down your jars before you put them in you SAB

wipe them again inside your SAB.

you can spray some lysol or bleach water. 

let the air settle, and get to work.

wear gloves, flame tip your needle or scalpel. etc

:thumbup:


--------------------
I could go crazy... and no one would notice :seeya:

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InvisibleSkinty
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Registered: 07/04/13
Posts: 1,150
Re: Hi all! PF tek beginner here [Re: Switchback]
    #20381864 - 08/06/14 09:26 AM (10 years, 5 months ago)

As Quexl indicated your bathroom is likely the dirtiest room in a house no matter what lysol spraying regime you do :thumbup:


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OfflineSwitchback
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Re: Hi all! PF tek beginner here [Re: Skinty]
    #20395094 - 08/09/14 10:09 AM (10 years, 5 months ago)

Hey guys 9 days in, mycelium has taken off! All are looking good, no discolorations or anything, just some FAT mycelium inoculation points. :grin: I have 6 jars going right now I would have ten, but little overkill on the inoculation. As soon as these jars are ready to be birthed I plan on taking 5 of these and finishing them off as cakes. As for the other one I was thinking of breaking it up and splitting it up in rye jars. or possibly breaking it up and putting it in a monotub. Thoughts?


Thanks!

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OfflineBurberrySpores
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Re: Hi all! PF tek beginner here [Re: Switchback]
    #20395180 - 08/09/14 10:40 AM (10 years, 5 months ago)

Pics or it didn't happen


--------------------
I could go crazy... and no one would notice :seeya:

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OfflinePrincessPeach
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Re: Hi all! PF tek beginner here [Re: BurberrySpores]
    #20395329 - 08/09/14 11:14 AM (10 years, 5 months ago)

So like I did 12 jars my first run. I was clean about it, but I didn't sterilize the needle between holes or even turn my a/c off or anything like that. All 12 of my jars made it. I don't believe it's necessary to go sterile crazy, just be smart about. Wash your hands, any thing your working with, any counter your using. Don't touch stuff if you don't need to etc. Also I only boiled my jars for an hour. I think extremes like this are only necessary when you know you have an existing issue in your environment with contamination.


--------------------

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OfflineJman420
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Re: Hi all! PF tek beginner here [Re: PrincessPeach]
    #20395335 - 08/09/14 11:16 AM (10 years, 5 months ago)

I think everyone should start out with the monotub tek. :shrug:
Much better results, always.

Gl


--------------------
            --My Everything You Need To Know Tek--

Disregard the haters and "know it alls", it is everything you need to know TO GET STARTED.

If you want to become an expert, you'll need to do a lot more research.
-----------------------------------------------------------------------
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OfflineSwitchback
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Re: Hi all! PF tek beginner here [Re: Jman420]
    #20469424 - 08/24/14 11:06 AM (10 years, 4 months ago)

Hey guys, 24 days later and I would assume the Jars are fully colonized, oh yeah I lied I actually have 8 Jars I know I stated 6. Anyways the jars.. 4 are all white no contamination visible, I plan on giving them a few more days before they enter a FC, as for the other four they seem to be stalled. The mycelium is still colonizing but unbelievably slow. I ordered some supplies from outgrow and I got 2 free Filter discs thrown in, (Thanks!) so I took the lid off of one and placed a filter disk on top possibly to get some fresh air in there? I read its possible that they need a little more FAE than what they have been getting.

The 4 will go into a tote with perlite ect... and I have a aquarium air pump to be pumping constant fresh air into it, so no fanning will be necessary???   


what would you guys recommend for fruiting temperature, I have read a lot and most is contradicting..

One more thing, I am not going to post pictures yet, I will when its all said and done

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InvisiblebodhisattaMDiscordReddit
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Re: Hi all! PF tek beginner here [Re: Switchback] * 1
    #20469442 - 08/24/14 11:10 AM (10 years, 4 months ago)

:justnotright:
Quote:

The 4 will go into a tote with perlite ect... and I have a aquarium air pump to be pumping constant fresh air into it, so no fanning will be necessary???   




never use an air pump. They DO NOT replace fanning at all.

you need to do some reading big time.(click the first link in my signature)

Fruit between 60-100F
colonize between 60-78F(cubensis mycelium slows down at an internal temp of 82F which is reached by about 78F outside of the jars)
room temperature is fine for all aspects of growing cubensis, if your room temperature is above 60F and below 80F then don't try to do anything special.

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OfflineQuexl
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Re: Hi all! PF tek beginner here [Re: Switchback]
    #20469458 - 08/24/14 11:13 AM (10 years, 4 months ago)

:ohnowaitokay::D

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Offlinebeerdedguy
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Registered: 08/10/14
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Re: Hi all! PF tek beginner here [Re: Jman420]
    #20469463 - 08/24/14 11:15 AM (10 years, 4 months ago)

Quote:

Jman420 said:
I think everyone should start out with the monotub tek. :shrug:
Much better results, always.

Gl



That's what I did. I was going to just do the brf cakes in a sgfc but decided to do a monotub instead.

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InvisiblebodhisattaMDiscordReddit
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Re: Hi all! PF tek beginner here [Re: bodhisatta] * 1
    #20469472 - 08/24/14 11:16 AM (10 years, 4 months ago)

91% alcohol doesn't work as good as 70% alcohol for sanitation. save your 91% for cleaning pipes.

The bathroom is dirty as fuck don't do any mycology work in a bathroom.

as soon as sterilization is done the foil comes off and stays off. you don't want the foil to stay on unless you want contamination to grow in the dry vermiculite layer.

lysol does shit for making a room clean, ditch lysol never buy it again unless you're cleaning your bathroom and even then lysol sucks compared to some ammonia and water.

build a SAB. You only need to spray the inside of a SAB with very diluted dish soap water. 1 drop soap to a quart of water. Still air box not sanitary or sterile air box. The still air is what keeps your work sterile, the inner surfaces of a SAB could be dirty as shit but the still air is what's protecting you. Don't spray lysol or alcohol inside of a SAB.

:picdidnthappen:

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OfflineLumpyNutz
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Re: Hi all! PF tek beginner here [Re: PrincessPeach]
    #20469584 - 08/24/14 11:36 AM (10 years, 4 months ago)

Quote:

PrincessPeach said:
So like I did 12 jars my first run. I was clean about it, but I didn't sterilize the needle between holes or even turn my a/c off or anything like that. All 12 of my jars made it. I don't believe it's necessary to go sterile crazy, just be smart about. Wash your hands, any thing your working with, any counter your using. Don't touch stuff if you don't need to etc. Also I only boiled my jars for an hour. I think extremes like this are only necessary when you know you have an existing issue in your environment with contamination.




I use the same sterilization/sanitation technique and have very few issues with contam!:thumbup:

Also I:
  1) WIPE NEEDLE WITH A PAPER TOWEL WITH RUBBING ALC ON IT!
  2) Flame sterilize needle
  3) Innoc 1 jar (all 4 holes)
  4) ...Repeat steps 1-4

Is this the proper technique???


--------------------
This is uh... no offence... but you are a robot aren't you?


GET SHPONGLED

Edited by LumpyNutz (09/06/14 04:24 PM)

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InvisiblebodhisattaMDiscordReddit
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Re: Hi all! PF tek beginner here [Re: LumpyNutz] * 1
    #20469597 - 08/24/14 11:39 AM (10 years, 4 months ago)

no you wipe then flame.
alcohol = sanitary
flame = sterile

don't work backwards

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InvisiblebodhisattaMDiscordReddit
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Re: Hi all! PF tek beginner here [Re: bodhisatta]
    #20469630 - 08/24/14 11:45 AM (10 years, 4 months ago)

Quote:

RogerRabbit said:
Wiping with alcohol after flame sterilizing is like wiping shit on your face after taking a shower.
RR



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OfflineBumbaclotjohnson
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Re: Hi all! PF tek beginner here [Re: bodhisatta]
    #20472187 - 08/24/14 09:28 PM (10 years, 4 months ago)

Quote:

bodhisatta said:
Quote:

RogerRabbit said:
Wiping with alcohol after flame sterilizing is like wiping shit on your face after taking a shower.
RR







This is by far my favorite rr quote I think about it randomly when inoculating jars.


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Edited by Bumbaclotjohnson (08/24/14 09:29 PM)

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