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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Agar to Grain (A2G) 9
#20380256 - 08/05/14 08:42 PM (9 years, 5 months ago) |
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This is part of my series "The Basics"
This is my first grow since loosing all my cultures and spores due to flooding about 9 months ago.
Once you have your grains prepared, you need to inoculate the grains. Once 100% colonized, it becomes "Grain Spawn". Grain spawn, aka spawn, is usually mixed with a bulk substrate, allowed to colonize again, and then fruited. This process is often refered to as "Spawning To Bulk". In my case, I will be spawning to bulk in a monotub.
There are different ways and grains for making spawn. Many people, especially beginners, use a syringe with spores (aka MS or Multispore).
In this write-up, I used a colonized agar wedge to inoculate Rye Berries. I don't want to spend too much time explaining agar because I really just wanted to show what what spawn looks.
Here's what I did to make this grain spawn:
On 2014-07-19, I began with a single drop of MS solution on a single agar plate. I've gone through about 50ish plates so far trying to get limited, clean, and healthy looking mycelium.
I have it narrowed down to 12 plates I will be growing out over time. Each plate is 4 transfers from MS. Not an isolate, but "limited" genetics.
I didn't get a pic of the agar plate because of condensation.
Here is the first A2G, from the new beginning, using my Rye Berry Tek.
The agar recipe is: 10g agar agar 8g LME 500ml water.
Be careful when dropping in your wedge. These things really stick the glass and are almost imposable to get unstuck. You don't have to shake the jar right away. What I do is, once the lid is closed, I roll it around a little to get some grains to cover it. Then, I just let it be. It doesn't really matter if the agar sticks to the glass as long as it's covered with some grain.
Day 1
Drop agar wedges, from a single petri, in to 6 jars

Day 3
The mycelium has mostly covered the agar wedges and is colonizing the grains.

Day 5
Mycelium is definantly on the grains. Starting to see rhyzomorphic mycelium similar to what was on the agar plate.

I'll probably give them a good shake in a couple of days. I will post an update at shake time.
Day 7
Before and after shaking. The clusters around the agar are bigger than they appear in the pic. They broke up really easy with some shaking. No banging required. Got it all good and spread out.

Day 12

Sidenote: I may make an adjustment to the dry time on my grain prep. I just wanted to mention this tek is meant to be beginner friendly and by default errs on the dry side. A slightly wetter grain would colonize faster, but, it's easy to get too wet, so adjust with caution.
Day 13 I went ahead and gave the jars another shake. They broke up very easily with a couple little bounces on my bike tire and then some shaking.

My next update will be at 100% colonization.
I'll update as I go.
Coming soon: Making Bulk Substrate and Spawning to bulk
Edited by SpitballJedi (08/18/14 07:47 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Gonna be on board for this one 
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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I can feel the pressure to succeed. But, you just never know with certainty.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
Edited by SpitballJedi (08/05/14 08:49 PM)
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shamanamba
The Shaman



Registered: 06/15/05
Posts: 1,122
Last seen: 1 month, 17 days
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-------------------- The Shaman
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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50 plates from one single spore drop?!?!? damn u must be after something nice!!
yes, I'm watching this
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Quexl


Registered: 12/17/13
Posts: 1,443
Loc:
Last seen: 8 years, 10 months
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Doing the same thing myself right now  Looking forward to seeing how it progresses for ya.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Quote:
blindingleaf said: 50 plates from one single spore drop?!?!? damn u must be after something nice!!
yes, I'm watching this

I kept getting a lot of tomentose growth. Some plates got 1-4 transfers from from them. That first plate got 6 transfers. I may have went a little over-board. But it just agar plates.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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BLINKfan420
Jedi



Registered: 07/06/12
Posts: 1,078
Last seen: 4 months, 30 days
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BLiNK
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  All my pictures are drawn from imagination. I don't even know what a mushroom is.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 19 days
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Quote:
SpitballJedi said:
Quote:
blindingleaf said: 50 plates from one single spore drop?!?!? damn u must be after something nice!!
yes, I'm watching this

I kept getting a lot of tomentose growth. Some plates got 1-4 transfers from from them. That first plate got 6 transfers. I may have went a little over-board. But it just agar plates.
What is wrong with tomentose growth? Dosen't mean that the strain is weak. Doesn't mean that the agar is lacking nutrients. Just means that the germination process is successful.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Agar to Grain (A2G) [Re: Sockadin]
#20380445 - 08/05/14 09:33 PM (9 years, 5 months ago) |
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Quote:
Sockadin said:
Quote:
SpitballJedi said:
Quote:
blindingleaf said: 50 plates from one single spore drop?!?!? damn u must be after something nice!!
yes, I'm watching this

I kept getting a lot of tomentose growth. Some plates got 1-4 transfers from from them. That first plate got 6 transfers. I may have went a little over-board. But it just agar plates.
What is wrong with tomentose growth? Dosen't mean that the strain is weak. Doesn't mean that the agar is lacking nutrients. Just means that the germination process is successful.
Nothing wrong with it. I just can't, as easily, tell if it's cube myc or something else, especially if it's also thin.
And it doesn't just mean the germination process was successful, it can also mean you have multiple genetics growing together. I wanted to narrow the genetics.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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Abshroom
Cause he can


Registered: 06/05/14
Posts: 810
Loc: Germany
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Nice one. Will monitor this thread have my fist serious work with agar also on going hope It will succeed and of course I hope yours will succeed as well
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Updated the OP:
Day 3
The mycelium has mostly covered the agar wedges and is colonizing the grains.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Tomentose can turn in normal rhizo and rhizo back to tomentose. It have something to do with nutrient levels. Some had great success with tomentose wedges.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Agar to Grain (A2G) [Re: Mr. Alien]
#20393131 - 08/08/14 07:21 PM (9 years, 5 months ago) |
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Cool stuff! 
How many transfers did you do from the orgininal swipe plate? I've started to keep some old dishes lying around thru the grow after one I forgot about grew mold just a couple days after my bulk sub did.
So now I just do lots of transfers no matter how clean the 1st or 2nd plates after transfer looks..
rant out in
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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Re: Agar to Grain (A2G) [Re: Mr. Alien]
#20393184 - 08/08/14 07:35 PM (9 years, 5 months ago) |
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Quote:
Mr. Alien said: Tomentose can turn in normal rhizo and rhizo back to tomentose. It have something to do with nutrient levels. Some had great success with tomentose wedges.
Someone in Dilated's agar ride along thread (I think that's where I read it) mentioned that you can use less nutrients in your agar recipe to encourage rhizo growth. I went from using equal parts agar:elme to using a 10g:9g ratio and have more rhizo looking growth since i cut back the malt.
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 19 days
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Re: Agar to Grain (A2G) [Re: MrGiraffe]
#20393342 - 08/08/14 08:16 PM (9 years, 5 months ago) |
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I just made 4 masters out of Tomentose Costa Rican Cube plates. It is all about the fruiting conditions.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,918
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Re: Agar to Grain (A2G) [Re: Sockadin]
#20393860 - 08/08/14 10:59 PM (9 years, 5 months ago) |
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Woohoo Jedi! Is it cool if I call you Jedi?
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PsiloBeast
Stonesun Wannabe



Registered: 05/23/14
Posts: 2,517
Loc: The Wild
Last seen: 9 years, 4 months
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Welp, I'm definately hopping on the SBJ train too.
-------------------- Whosoever is delighted in solitude is either a wild animal or a god... -Aristotle A COMPLETE GROW LOG (ATL# 7) BEAST STYLE LINKS Water Extraction Tek,Frank's Monotub Tek ,Do What He Does...
 
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Quexl


Registered: 12/17/13
Posts: 1,443
Loc:
Last seen: 8 years, 10 months
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It's jumped over man, good on you, just let it take overrrrr.
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Stuey1
We're all mad Here



Registered: 11/28/13
Posts: 740
Loc: Bat Country
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Re: Agar to Grain (A2G) [Re: Quexl]
#20394151 - 08/09/14 12:48 AM (9 years, 5 months ago) |
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I'm here for this as well.
-------------------- Have you ever danced with the devil in the pale moon light?
 
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