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OfflinemikeyASD
b00p
Registered: 10/21/03
Posts: 23
Last seen: 20 years, 3 months
inoculating without syringe
    #2035449 - 10/23/03 07:39 AM (20 years, 5 months ago)

If someone is ordering spore PRINTS from overseas..
After a jar is steralised, is it possible to just scrape some of the spores ontop of the cake and then put the 1/2" layer of dry Verm on top of that?
Or is it completely necessary to use a syringe to inoculate?
If so, where do you purchase syringes that hold 10cc's ?
Thanks :smile: 


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:smile:

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Anonymous

Re: inoculating without syringe [Re: mikeyASD]
    #2035458 - 10/23/03 07:43 AM (20 years, 5 months ago)

i believe the spores need to be rehydrated. You can buy syringes from a number of the sites on the shroomery vendor page.

~J-Slice~

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InvisibleTomandjerry58
Stranger
Registered: 01/27/03
Posts: 5,212
Re: inoculating without syringe [Re: ]
    #2035460 - 10/23/03 07:45 AM (20 years, 5 months ago)


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OfflinemikeyASD
b00p
Registered: 10/21/03
Posts: 23
Last seen: 20 years, 3 months
Re: inoculating without syringe [Re: Tomandjerry58]
    #2035490 - 10/23/03 08:02 AM (20 years, 5 months ago)

I cant buy/order syringes online as I am from Australia and syringes dont make it through customs (only prints)
So I have to make my own spore syringe (which sucks as i am a newbie and that will probably be the hardest bit, sterility wise)
Does anybody know where in Australia I can purchase 10cc Syringes "with a long needle" ?
Thanks :smile:


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:smile:

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InvisibleTomandjerry58
Stranger
Registered: 01/27/03
Posts: 5,212
Re: inoculating without syringe [Re: mikeyASD]
    #2035493 - 10/23/03 08:06 AM (20 years, 5 months ago)

its kind f hard to believe that a syringe with nothing in it. could not make it through customs.

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OfflinemikeyASD
b00p
Registered: 10/21/03
Posts: 23
Last seen: 20 years, 3 months
Re: inoculating without syringe [Re: Tomandjerry58]
    #2035520 - 10/23/03 08:30 AM (20 years, 5 months ago)

Apologies - I assumed you meant 'just order a spore syringe'
My bad!


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:smile:

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Invisiblepsilomonkey
Twisted brainwrong of a oneoff man mental

Registered: 08/08/03
Posts: 812
Loc: Airstrip One
Re: inoculating without syringe [Re: mikeyASD]
    #2035536 - 10/23/03 08:41 AM (20 years, 5 months ago)

You could use a sterile eye dropper, but the risk of contams will be a lot higher as you would have to open the cake to air. If you really can't get hold of an empty syringe try looking at inkjet refill kits. They have a syringe (blunted needle, but that does not matter).

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InvisibleTomandjerry58
Stranger
Registered: 01/27/03
Posts: 5,212
Re: inoculating without syringe [Re: psilomonkey]
    #2035547 - 10/23/03 08:48 AM (20 years, 5 months ago)

its all cool:) i didn't specify. i was looking for the link but my computer froze on there website. go to the online store i believe its under lab supplies.

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OfflinemikeyASD
b00p
Registered: 10/21/03
Posts: 23
Last seen: 20 years, 3 months
Re: inoculating without syringe [Re: Tomandjerry58]
    #2035561 - 10/23/03 08:56 AM (20 years, 5 months ago)

I know where to buy 5ml syringes
but then i wouldnt know how much spore solution to inject into the jar.
Can you put too much?
As long as I dont change the water percentage (greatly) it should be ok if i just squirt a few drops into each hole?


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:smile:

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Invisiblepsilomonkey
Twisted brainwrong of a oneoff man mental

Registered: 08/08/03
Posts: 812
Loc: Airstrip One
Re: inoculating without syringe [Re: mikeyASD]
    #2035576 - 10/23/03 09:06 AM (20 years, 5 months ago)

If you can see a few dark flecks in the syringe (thouse are massive clumps of spores.. thoowwsands ov 'em), the rest of the solution will have millions of tiny spores you can't see. 1/4 ml is what I used per hole. Its does pay to draw some air through a flame into the syringe so you can shake the spores and even things out.
From a dark 1" print I make about 20 syringes.

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Invisiblestarseed
professional lurker

Registered: 09/18/03
Posts: 671
Re: inoculating without syringe [Re: mikeyASD]
    #2036568 - 10/23/03 02:24 PM (20 years, 5 months ago)

i have tried this scraping method..
it works, but the jars take forever to fully colonize..
i did this to 6 jars, i lost 3 to contams, and the rest took 2 months to complete. when you just scrape the spores over the top, it takes a long time for the mycelium to get all the way to the bottom.. if you do use this methid, i would strongly suggest a layer of verm on the bottom of the jar.


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:poison::poison:                                  :poison::poison::poison:                                  :poison::poison:

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InvisibleTomandjerry58
Stranger
Registered: 01/27/03
Posts: 5,212
Re: inoculating without syringe [Re: starseed]
    #2036580 - 10/23/03 02:28 PM (20 years, 5 months ago)

a hole print should make about four good syringes. check some of the faqs out for making syringes. i could tell you but you would fuck it up then so read the faqs.

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OfflineTold
Artiest

Registered: 03/09/03
Posts: 343
Loc: Rockies
Last seen: 19 years, 7 months
Re: inoculating without syringe [Re: Tomandjerry58]
    #2036961 - 10/23/03 04:48 PM (20 years, 5 months ago)

what would you do if i said your crazy? cause you are. you need a syringe.

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OfflinemikeyASD
b00p
Registered: 10/21/03
Posts: 23
Last seen: 20 years, 3 months
Re: inoculating without syringe [Re: Told]
    #2037063 - 10/23/03 05:14 PM (20 years, 5 months ago)

It was just a question.
But thanks for replying :smile:


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:smile:

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OfflinemikeyASD
b00p
Registered: 10/21/03
Posts: 23
Last seen: 20 years, 3 months
Re: inoculating without syringe [Re: mikeyASD]
    #2037069 - 10/23/03 05:16 PM (20 years, 5 months ago)

And just thought i'd let you all know..
I ordered my Spore prints this morning. HOW EXCITING!%@%#%$
:P
*hopes they arrive*
Lets all try and pick out the newbie. *grin*


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:smile:

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OfflineVertigo
Mycovoire
Registered: 03/05/03
Posts: 289
Loc: Panhandle
Last seen: 5 years, 7 months
Re: inoculating without syringe [Re: mikeyASD]
    #2037095 - 10/23/03 05:24 PM (20 years, 5 months ago)

Look in a grocery store for "flavor injectors" i.e. a syringe.


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"One side will make you grow taller, and the other side will make you grow shorter."

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