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OfflineMrcloudy
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Re: Reishi spore extract Triterpene crystals [Re: forrest]
    #20331313 - 07/26/14 09:42 AM (9 years, 6 months ago)

They have several methods, one uses enzymes followed by mechanical breaking. They use rolling machines, high pressure steam, and I think ultrasound. The interesting one is the one where they seem to jump straight to extraction.

Quote:

Manufacturing Example 5

1. One hundred (100) kg of mature spores of red Ganoderma lucidum were carefully selected and 100 kg of a culture solution of 0.1% potassium phosphate (monobasic) and magnesium sulfate was added to the spores to carry out a soaking treatment to induce germination for 8 hours, with the temperature of the solution set at 21° C.

2. After the soaking, the excess solution was eliminated by allowing it to drip, and the spores were placed in a well-ventilated culturing box at a constant temperature and humidity to carry out activation culture. The relative humidity of the culturing box was 85%, the temperature at 42° C., and the activation period lasted for 3 hours. When examined under the electron microscope, it was found that the spores of red Ganoderma lucidum were in a state of activation, the formation of buds had occurred and the epispores were clearly softened during the activation period.

3. Extraction was carried out with water, followed by a second extraction with alcohol. The extract solutions were combined and concentrated using a thin film technique.

4. Further processing was carried out to make purified powders, extract pastes, solutions for injection or oral consumption.




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10 different Ganoderma species from across the USA

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MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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Offlinepetri_pins
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Re: Reishi spore extract Triterpene crystals [Re: Mrcloudy]
    #20331805 - 07/26/14 11:48 AM (9 years, 6 months ago)



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OfflineMrcloudy
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Re: Reishi spore extract Triterpene crystals [Re: petri_pins]
    #20331939 - 07/26/14 12:16 PM (9 years, 6 months ago)

Oh wow that looks like it absolutely destroys the spores. I wonder if they did any testing to ensure the medicinal compounds weren't consumed by the lactobacteria.


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10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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OfflineEuphie
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Re: Reishi spore extract Triterpene crystals [Re: petri_pins]
    #20332072 - 07/26/14 12:47 PM (9 years, 6 months ago)

Quote:

petri_pins said:
I found a culture tek using bacteria.
http://www.academicjournals.org/article/article1380733449_Chaiyasut%20et%20al.pdf





I wouldn't think its really necessary to break them down so much.


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OfflineMrcloudy
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Re: Reishi spore extract Triterpene crystals [Re: Euphie]
    #20332292 - 07/26/14 01:33 PM (9 years, 6 months ago)

I think it probably allows for more efficiency when extracting due to more surface area. I do think that as long as they have been germinated and the spore wall has been compromised then the medicinals should be extractable. It may take a little longer than fully broken spores but I do think it should work.


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10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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OfflineMrcloudy
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Re: Reishi spore extract Triterpene crystals [Re: Mrcloudy]
    #20334183 - 07/26/14 07:24 PM (9 years, 6 months ago)

So, this morning there was no germination, so I took the jar with the spores in it and placed it over the hole in the middle of my dehydrator with the top off to provide heat. It was there for 4-6 hours I did not reaally keep track. I just looked at it under the microscope and it took a bit of searching but eventually I found two spores that had fully germinated and had mycelial strands coming off from them. Just two. I also noticed a rather unpleasant pondwater type smell that I presume is some bacteria that has also germinated.:shrug: 

Not sure if i should coninue with the experiment or not. I plan on boiling the mixure at the end to extract the spore goodies into the water which should kill any residual bacteria. But still thats a little dissapointing. I was hoping such a simple quick procedure wouldn't need the same kind of cleanliness as full blown cultivation. Something you could do in a 5 gallon bucket in your kitchen. Might have been better though had I started from dry spore instead of several day old spore syringe using non sterile water.


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10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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Offlinepetri_pins
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Re: Reishi spore extract Triterpene crystals [Re: Mrcloudy]
    #20335126 - 07/26/14 10:34 PM (9 years, 6 months ago)

What about trying a boil? The spores are tuff. Maybe the high heat then add some sugar and jar it and if they are so tuff the boil might help germination? I have no scope to see this though :shrug: I hope to have one as soon as I can.

I also thought maybe pressure cooking? If you cook it for a long time in the pc maybe the pressure will do it? I would go for overkill first time with heat and length of time. Then dial it back to find the minimum heat and time if the results are favorable.

I'm going to look into this more and get going on some more reishi for some tests. Mrcloudy keep those tests going if you got the spores and keep us updated.


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OfflineMrcloudy
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Re: Reishi spore extract Triterpene crystals [Re: petri_pins]
    #20335168 - 07/26/14 10:47 PM (9 years, 6 months ago)

I will keep doing experiments. I suppose I should portion out the spores, I dont really need to do tests on millions at a time. I do intend to keep conking my reishi and collecting as many spores as possible. This is an interesting concept and I am excited to experiment with it.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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Offlinedrake89
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Re: Reishi spore extract Triterpene crystals [Re: Mrcloudy]
    #20335194 - 07/26/14 10:56 PM (9 years, 6 months ago)

100kg spores !?  Woah...


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OfflineMrcloudy
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Re: Reishi spore extract Triterpene crystals [Re: drake89]
    #20335204 - 07/26/14 10:58 PM (9 years, 6 months ago)

Yeah they used a lot of spores. I guess since their idea is for industrial scale, their tests are industrial scale tests. Thats a lot of spores. A lot of mushrooms to get those spores.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


Extras: Filter Print Post Top
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