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InvisibleTheChief
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Experimental Inoculation W/Pics
    #20291199 - 07/18/14 12:48 PM (9 years, 7 months ago)

I went ahead and took it upon myself to do a little experiment in mycology, since I am a noob in training, what could it hurt?  11 days ago when a friend and I did the bulk to spawn step that our grow kits came with (see my first grow post for more info on this), I purposely took some of the rye spawn out of each of the half gallon jars they came with; put them in a bag, and stored them in the fridge...  I had an idea in my head... If I could save some of the fungus on the rye berries, I could probably transplant it to other jars and grow more of it (kind of like G2G but at the time I had no idea if it would work).  Later on that week, I went ahead and got the supplies required to make 10 half pint BRF cakes.  The following was what I did with the fungus in a step by step tek (I wouldn't recommend doing this yourself because your stuff will probably get contaminated, this is just an experiment).

1.  Made the standard BRF cake mixture
2.  Drilled standard inoculation holes in my 1/2 jars
3.  Filled my jars with the BRF mixture (leaving 1/2 inch at top)
4.  Fill 1 full pint jar with dry vermiculite
5.  Cover all the jars with foil
6.  PC at 15psi for 60 min
7.  let cool overnight
8.  Shut off HEPA in the house to stop any moving air
9.  Made sterile working area
10. Sterilized myself (mask, gloves, alc)
11. Proceed to remove jars from PC
12. Quickly and carefully, remove lid from each BRF jar, add spawn to top; then add dry layer of vermiculite and seal.
13. Let colonize

Mind you I worked very quickly and carefully trying to expose my sterilized materials to as little open air as possible.  It has now been several days since I did this inoculation, and all 10 jars have growth.  I have no idea if they will make it all the way, but I guess we'll see won't we? Here are a couple pics of the progress...




I know a lot of you would frown upon something like this, but it was all I had to work with; and at the time, I didn't know any better. 

Also here is a link to my first grow if you were curious:  http://www.shroomery.org/forums/showflat.php/Number/20281385

Sincerely,
The Chief


Edited by TheChief (08/08/14 10:21 PM)


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Offlinejerrylove
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Re: Experimental Inoculation W/Pics [Re: TheChief]
    #20291322 - 07/18/14 01:11 PM (9 years, 7 months ago)

That is a risky way to go but hey if you don't mind failing then what the he'll let er rip.


--------------------
:mushroom2:THOSE WHO KNOW DONT TALK!:mad:THOSE WHO DONT KNOW CANT TALK.:smile: (Timothy Leary):mushroom2:
"Funny thing about weekends when your unemployed they don't mean quit so much except you get hang out with your workin' friends" Les Claypool


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InvisibleTheChief
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Re: Experimental Inoculation W/Pics [Re: jerrylove]
    #20291343 - 07/18/14 01:13 PM (9 years, 7 months ago)

Well, I had a feeling that our kits may fail.  So, at the time, saving the spawn at any cost seemed like a good idea.


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jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."


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OfflineSits
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Re: Experimental Inoculation W/Pics [Re: TheChief]
    #20291356 - 07/18/14 01:16 PM (9 years, 7 months ago)

What was your procedure for taking the rye out of the original jars? I've never done a G2G but I know it's more contamination prone than say injecting spores, so I'd say contams are likely if you haphazardly transferred and stored your grains. I'm a rank amateur though, good luck!


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InvisibleTheChief
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Re: Experimental Inoculation W/Pics [Re: Sits]
    #20291373 - 07/18/14 01:19 PM (9 years, 7 months ago)

I did it by hand, with disinfected gloves and working area.

Edit:  Sorry, I completely gaped half your question.  To answer, I simply took the colonized berries out of the half gallon jars and quickly put them into a brand new zip-lock bag.  I stored them in a clean place in the fridge until I got materials to do cakes.  Probably wasn't the best procedure.  I thought about it after, if I would have sterilized another jar, I could have just put them in there.  But hey, I guess that's the way experiments go.


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jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."


Edited by TheChief (07/18/14 11:28 PM)


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InvisibleTheChief
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Re: Experimental Inoculation W/Pics [Re: TheChief]
    #20296611 - 07/19/14 02:09 PM (9 years, 7 months ago)

Bump.


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jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."


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OfflinecronicrFacebook
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Re: Experimental Inoculation W/Pics [Re: TheChief]
    #20296634 - 07/19/14 02:13 PM (9 years, 7 months ago)

is there a reason for your bump?


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleTheChief
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Re: Experimental Inoculation W/Pics [Re: cronicr]
    #20296685 - 07/19/14 02:25 PM (9 years, 7 months ago)

Quote:

cronicr said:
is there a reason for your bump?



Ya, It's been 24hrs and I edited my previous post for Sits.  Just wanted to make sure he saw.


--------------------


jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."


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OfflinecronicrFacebook
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Re: Experimental Inoculation W/Pics [Re: TheChief]
    #20296703 - 07/19/14 02:29 PM (9 years, 7 months ago)

:jackiechanofapproval:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleSpitballJedi
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Re: Experimental Inoculation W/Pics [Re: TheChief]
    #20296747 - 07/19/14 02:42 PM (9 years, 7 months ago)

You would have been better off leaving the grains in the original container for storage and then pouring them in to your cake jars.

If you have a filter, like SFD's for instance, you can skip the dry vermiculite barrier.

Do you have GE holes in your lids?


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


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InvisibleTheChief
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Re: Experimental Inoculation W/Pics [Re: SpitballJedi]
    #20296757 - 07/19/14 02:44 PM (9 years, 7 months ago)

Quote:

SpitballJedi said:
You would have been better off leaving the grains in the original container for storage and then pouring them in to your cake jars.

If you have a filter, like SFD's for instance, you can skip the dry vermiculite barrier.

Do you have GE holes in your lids?



Sure do.  I'm looking into getting some filters for my jars.


--------------------


jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."


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InvisibleTheChief
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Re: Experimental Inoculation W/Pics [Re: TheChief]
    #20296837 - 07/19/14 03:06 PM (9 years, 7 months ago)

Wait scratch that.  I just searched what a GE hole was and NO I don't have them.  I just drilled holes in the lid like the PF tek said to do.


--------------------


jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."


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InvisibleTheChief
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Re: Experimental Inoculation W/Pics [Re: TheChief]
    #20296847 - 07/19/14 03:08 PM (9 years, 7 months ago)

There is a bit more growth today.  How long would you say it should take for 100%?


--------------------


jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."


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InvisibleSpitballJedi
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Re: Experimental Inoculation W/Pics [Re: TheChief]
    #20296873 - 07/19/14 03:14 PM (9 years, 7 months ago)

Quote:

MattDiGi said:
Wait scratch that.  I just searched what a GE hole was and NO I don't have them.  I just drilled holes in the lid like the PF tek said to do.




:thumbup: Those are GE holes.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


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InvisibleTheChief
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Re: Experimental Inoculation W/Pics [Re: SpitballJedi]
    #20326553 - 07/25/14 11:37 AM (9 years, 6 months ago)

Update:  These jars seem to be doing well.  I tossed two of them because one had some green growth spot and another one I thought just wasn't doing anything, I couldn't see white growth... Turns out it was growing in the center.  That was two days ago, the other 8 are all white. Here are some pics.  Also, I realized they are only 125ML (1/2 of 1/2 pt) jars, I guess we have some mini cakes.  I was wondering why I had so much BRF mix left over...  :wow:





Edited by TheChief (07/25/14 06:21 PM)


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OfflineDonsanpedro
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Re: Experimental Inoculation W/Pics [Re: TheChief]
    #20326695 - 07/25/14 12:22 PM (9 years, 6 months ago)

Yea those are 4oz jars, i used some in a pinch.  IMO they won't really produce cakes big enough to get good fruits off. 

I had to go to like 18 stores to find 1/2 pint wide mouth jars, but they are so worth it.

I think you did the right thing trying to save some myc :thumbup:
DSP


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InvisibleTheChief
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Re: Experimental Inoculation W/Pics [Re: Donsanpedro]
    #20326714 - 07/25/14 12:28 PM (9 years, 6 months ago)

Quote:

Donsanpedro said:
Yea those are 4oz jars, i used some in a pinch.  IMO they won't really produce cakes big enough to get good fruits off. 

I had to go to like 18 stores to find 1/2 pint wide mouth jars, but they are so worth it.

I think you did the right thing trying to save some myc :thumbup:
DSP



Well, kit 1# ended up succumbing to trich as well.  I put it out in a bush that seemed like a good growing spot for them.  Wen't back yesterday to water and a coon or something had a nice mess made of where I buried it;  must have been a good lunch.


--------------------


jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."


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OfflineKizzle
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Re: Experimental Inoculation W/Pics [Re: TheChief]
    #20327212 - 07/25/14 02:41 PM (9 years, 6 months ago)

Quote:

TheChief said:
Update:  These jars seem to be doing well.



:goodluckwiththat:


--------------------


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Offlineblojo02184
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Re: Experimental Inoculation W/Pics [Re: Kizzle]
    #20327355 - 07/25/14 03:16 PM (9 years, 6 months ago)

Quote:

Kizzle said:
Quote:

TheChief said:
Update:  These jars seem to be doing well.



:goodluckwiththat:




:wave: :lol:


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InvisibleTheChief
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Re: Experimental Inoculation W/Pics [Re: Kizzle]
    #20327416 - 07/25/14 03:27 PM (9 years, 6 months ago)

No love for baby cakes?  :gocrythen:


--------------------


jcbowling1985 said:
"Additionally I did one Armageddon jar with all of the types together."


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