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InvisibleFick_Duck
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Re: Experience with long-term storage in distilled water? [Re: ragadinks]
    #2032580 - 10/22/03 01:42 PM (16 years, 8 months ago)

2 and 1/2 years almost to the day, I was worried they were dead but they worked fine.


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Offlineragadinks
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Re: Experience with long-term storage in distilled water? [Re: Fick_Duck]
    #2033366 - 10/22/03 05:22 PM (16 years, 8 months ago)

Wow, that's really long!
What kind of mushroom was it, and how did you store the spore print ?


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Invisiblemycofile
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Re: Experience with long-term storage in distilled water? [Re: ragadinks]
    #2036442 - 10/23/03 01:41 PM (16 years, 8 months ago)

5 years for prints, worked fine.
5 years for syringe, still viable, but less germination
5 months in distilled water/h2o2
2 years in distilled water

Those are my longest saves. Of that, I see h2o2 as not being helpful. Just use water. The 5 months above was on something I was planning on using in a few days, but forgot about for months.

Also, on the method in general, I've found it very hard to get mycelium off of agar without pulling up some of the agar as well. If you get any of the agar into the water, you are defeating the point. You need to leave all nutrients out of the water (this is why it won't work to store karo forever). I've found forceps to work better for collecting mycelium from agar than a syringe. Also, if your agar is in a jar, once it's completely colonized the myc often starts climbing the walls of the jar. Grab these pieces with the forceps. You won't be putting any nutrients in hte water, therefore ensuring the longest possible storage.


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OfflineAnnoA
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Re: Experience with long-term storage in distilled water? [Re: mycofile]
    #2036486 - 10/23/03 01:58 PM (16 years, 8 months ago)

I had also stored small cubes of colonized agar....time to try to bring them back to life.


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Offlineragadinks
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Re: Experience with long-term storage in distilled water? [Re: mycofile]
    #2036507 - 10/23/03 02:03 PM (16 years, 8 months ago)

Wow, that's the record :shocked:
As I see it the spore prints last the longest,
followed by spores in distilled water,
followed by myc in distilled water.

The more nutrients are in the media the shorter the "shelf time" !?
Maybe we should try to store the myc in vacuum ?

Thanks for sharing your experiences ... 


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Offlineforevrgrounded98
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Registered: 10/25/03
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Re: Experience with long-term storage in distilled water? [Re: ragadinks]
    #2042955 - 10/25/03 08:43 PM (16 years, 8 months ago)

so dumb this down a shade for me please. I can take colonized cakes, stick them, submerged, in room temperature distilled water and they'll last for years?


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InvisibleCow Shit Collector
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Registered: 02/14/01
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Re: Experience with long-term storage in distilled water? [Re: forevrgrounded98]
    #2043007 - 10/25/03 09:16 PM (16 years, 8 months ago)

No.
The point is that the mycelium, having no nutrients/ oxygen, goes into a dormant state in the water vials.

Poor Man's Syringe Tek may be a good start/ implementation for those without agar. IMO


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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