|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
cowsRmeat
Don't step on the MomeRaths



Registered: 04/23/14
Posts: 3,153
Loc: Wonderland
|
My first go-around
#20257217 - 07/11/14 09:36 AM (10 years, 6 months ago) |
|
|
Hey all, first off, a big thanks to everyone at the shroomery for the wealth of info found here! You guys make it possible for us noobs to learn the ropes of the hobby and get some of our own projects going! So...thanks 
That being said, here is my first attempt. I kinda had a challenge before I even got started, as I am unable to get spores or syringes shipped to where I live. So... out I went to find my own specimens to get my own spores. I began researching here on the shroomery about how, when, and where to locate what I was looking for and, lucky for me, I found some on only my second trip out to the fields!

So as soon as I got em home, I ripped out some tin foil and laid the few largest caps out on the foil and left em laid out in the living room on a table. At the time, my knowledge of sterile procedure was EXTREMELY limited as far as printing goes, so I didnt do anything sterile at this point. I just popped off th caps, laid em on the foil, and left them out in open air.
The next day, I made my syringes up. I got some empty syringes and needles from a feed store. Here, my sterile procedure was a little better. I sterilized syringes with boiling water and then once cool I scraped my ENTIRE sheet of foil (3 caps' prints) into a little bowl (again in open air... I didnt know what I was doing... I did however, wipe down the bowl with alcohol first). And added the water from the syringes, of which I had two, swirled it around and slurped it back up into the syringes.
Now, with everything ready, supplies in hand, I prepared to start with the good ol PF tek. I mixed up everything according to one of the many teks here on the site (cant for the life of me remember which one, although there isnt a lot of variation in the pf teks I guess). Then I sterilized by boiling.
After they were cool, I innocculated the jars, this too was done in open air in the kitchen and needles were flamed with a lighter. I had two 12cc syringes and used 2cc per jar (yeah, i now know thats a lot for 1/2pt jars). I labelled my jars as to what syringe i used, date, etc. Jars were knocked up on 5/24.
Fast forward to 5/28 and there was all kinds of growth in the jars. All my jars from syringe number 2 was almost fully colonized... which I was pretty sure wasn't right, but I kept them around anyway (isolated of course). Jars from first syringe showed varying types of growth. A couple of em had some myc but also green mold, one of em had some pink stuff goin on, and the others looked like nothing but healthy myc.
Jars from syringe 2. All contam. I think cobweb mold? Can anyone confirm?

Jar with pink mold. When I emptied it out the surface under the lid was completely coated with pink wispy growths.

And here are some of the green growths

And finally, I had 3 healthy uncontam jars left to work with

So, yes that is a horrible contam rate of 75% contam, but I was proud I had ANY clean jars since this was my first try and (since I was reading more and more each day) I knew by this time that my sterile technique was seriously no good.
About 3.5-4 weeks later, my 3 jars were finished colonizing and consolidating, so I birthed them, dunked and rolled and put into my fruiting chamber. (The 3 jars were birthed over a span of like 5 days due to them colonizing at different rates). I used a SGFC I made and used like 5 in perlite. After a couple days in the fruiting chamber, pins started appearing and from there, I was surprised how quickly the fruits grew.
Here is the first cake pinning (over the course of a day or so)

Here is the first cake first flush

Second cake at first flush

The third cake had a lot of fruits on it but they were all small. I had pics of it and lots more of the rest of the process but my phone decided to go swimming recently and most of my pictures were lost with it (sadly, my phone didnt pay attention at swimming class and drowned due to its inexperience in the water lol)
So far, one cake contamed after two flushes, and the other two are on their third. Total yeild dry so far is like 14gs.
So, thats my first attempt with the pf tek, I definitely want to start into grains soon, as I now have a pc, sab, and a lot more reading under my belt, not to mention a little experience. I think with improvement with my sterile procedures I will have better results next time.
So, comments? Suggestions? Tips? I know it could have gone a lot better but I am kinda happy with myself for getting anything at all out of a WILD MS for my first try...
-------------------- One day Alice came to a fork in the road and saw a Cheshire cat in a tree.
'Which road do I take?' she asked.
'Where do you want to go?' was his response.
'I don't know', Alice answered.
'Then', said the cat, 'it doesn't matter.'
|
MHbound
Ballin Out At All Cost


Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
Last seen: 7 years, 9 months
|
Re: My first go-around [Re: cowsRmeat]
#20257239 - 07/11/14 09:44 AM (10 years, 6 months ago) |
|
|
That's really cool I have been wanting to do this for a long time. There is a field I go to that produces some really unusual fruits whether its the climate or location I'm not sure but I really want to bring them inside and see what the produce.
--------------------
|
Captainpaps
Babaji NA MA AUM



Registered: 11/24/13
Posts: 1,533
Last seen: 8 years, 5 months
|
Re: My first go-around [Re: cowsRmeat]
#20257240 - 07/11/14 09:45 AM (10 years, 6 months ago) |
|
|
Nicely done on getting something out of a wild ms spore print! Now you need to get into agar, and doing things with grains and bulk!
--------------------
|
das_shroom
Stranger

Registered: 07/07/14
Posts: 21
|
Re: My first go-around [Re: cowsRmeat]
#20257248 - 07/11/14 09:49 AM (10 years, 6 months ago) |
|
|
Seriously nice work!
It's fun to see things like this work out.
|
cowsRmeat
Don't step on the MomeRaths



Registered: 04/23/14
Posts: 3,153
Loc: Wonderland
|
|
Quote:
Captainpaps said: Nicely done on getting something out of a wild ms spore print! Now you need to get into agar, and doing things with grains and bulk!
Quote:
das_shroom said: Seriously nice work!
It's fun to see things like this work out.
Thank you kindly, folks. Yes, in the future, I plan on trying my hand at agar. In the meantime tho, I have taken prints from that second cake's fruits. I tried messing around with some brf substitute agar but I didnt give them the attention they needed. I made a small honey lc which is doing well. And I now have another set of pf jars going again at about 90% colonized and a dozen wbs qts going 10 off my homemade 2nd gen syringes and 2 off my honey lc. The lc jars are about 25% and the other wbs jars are at like 10-15%. And best of all-- 0 contams so far! Feel like I'm learning from my mistakes. Plan on doing a couple monos with the wbs and sgfc again for the pfs.
-------------------- One day Alice came to a fork in the road and saw a Cheshire cat in a tree.
'Which road do I take?' she asked.
'Where do you want to go?' was his response.
'I don't know', Alice answered.
'Then', said the cat, 'it doesn't matter.'
|
Captainpaps
Babaji NA MA AUM



Registered: 11/24/13
Posts: 1,533
Last seen: 8 years, 5 months
|
Re: My first go-around [Re: cowsRmeat]
#20257931 - 07/11/14 12:50 PM (10 years, 6 months ago) |
|
|
woa man well be careful with lc's created from a wild print, usually you put a wild print onto agar then separate it away from contams, with Lc you cant tell if it contaminated or not until you waste your materials and it shows up, when I do lc's from a syringe I put onto agar first then make lcs with the agar wedges I know are clean..
--------------------
|
cowsRmeat
Don't step on the MomeRaths



Registered: 04/23/14
Posts: 3,153
Loc: Wonderland
|
|
Quote:
Captainpaps said: woa man well be careful with lc's created from a wild print, usually you put a wild print onto agar then separate it away from contams, with Lc you cant tell if it contaminated or not until you waste your materials and it shows up, when I do lc's from a syringe I put onto agar first then make lcs with the agar wedges I know are clean..
Ok thanks for that. I have def read about lc needing to be tested for contams on a small scale to make sure it is clean. I just did it to compare germ rates between it and straight spore syringes. I only hit 2 jars with the lc for kicks and I already had honey lc ingredients in the house. I def have agar work in my future.
On a side note I hade made the lc off a print from one of the fruits that came from MY cakes, not the fruit I found wild. Is it still commonly still considered a 'wild' print at that point? If so, when would it no longer be considered 'wild', if ever?
-------------------- One day Alice came to a fork in the road and saw a Cheshire cat in a tree.
'Which road do I take?' she asked.
'Where do you want to go?' was his response.
'I don't know', Alice answered.
'Then', said the cat, 'it doesn't matter.'
|
Captainpaps
Babaji NA MA AUM



Registered: 11/24/13
Posts: 1,533
Last seen: 8 years, 5 months
|
Re: My first go-around [Re: cowsRmeat]
#20258019 - 07/11/14 01:22 PM (10 years, 6 months ago) |
|
|
If it was grown indoor and not outside its not a wild print, but if you take a wild print and grow it inside, then its possible to carry on the contaminants from the outdoor print onto your inside grows, that's why you wanna clean the print up with agar
--------------------
|
cowsRmeat
Don't step on the MomeRaths



Registered: 04/23/14
Posts: 3,153
Loc: Wonderland
|
|
Ah, I see. Thanks for clarifying that for me. What would you say is a more popular way to 'inoculate' agar --spores, or tissue sample?
-------------------- One day Alice came to a fork in the road and saw a Cheshire cat in a tree.
'Which road do I take?' she asked.
'Where do you want to go?' was his response.
'I don't know', Alice answered.
'Then', said the cat, 'it doesn't matter.'
|
Captainpaps
Babaji NA MA AUM



Registered: 11/24/13
Posts: 1,533
Last seen: 8 years, 5 months
|
Re: My first go-around [Re: cowsRmeat]
#20258103 - 07/11/14 01:49 PM (10 years, 6 months ago) |
|
|
I would say agar to grains, and then g2g transfers, that's what all the experience cultivators do
--------------------
|
cowsRmeat
Don't step on the MomeRaths



Registered: 04/23/14
Posts: 3,153
Loc: Wonderland
|
|
No, I meant actually starting up the agar. After it is all mixed up and poured into dishes or pc'd or whatever. Do most ppl use spores or tissue to start the actual agar dish.
Also, have you ever tried GLCs at all? Or would you say to just stick to g2g?
-------------------- One day Alice came to a fork in the road and saw a Cheshire cat in a tree.
'Which road do I take?' she asked.
'Where do you want to go?' was his response.
'I don't know', Alice answered.
'Then', said the cat, 'it doesn't matter.'
|
Captainpaps
Babaji NA MA AUM



Registered: 11/24/13
Posts: 1,533
Last seen: 8 years, 5 months
|
Re: My first go-around [Re: cowsRmeat]
#20258139 - 07/11/14 02:01 PM (10 years, 6 months ago) |
|
|
I've used spores off of foil onto agar, I've used spore syringes on agar, my previous attempts from doing tissue resulted in massive amount of cobweb on agar because they were outdoor grows, and I actually have some lc's going on right now, check out my journal
--------------------
|
PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
|
Re: My first go-around [Re: cowsRmeat]
#20258140 - 07/11/14 02:01 PM (10 years, 6 months ago) |
|
|
Quote:
cowsRmeat said: No, I meant actually starting up the agar. After it is all mixed up and poured into dishes or pc'd or whatever. Do most ppl use spores or tissue to start the actual agar dish.
Either will work......use what you have based on your expectations and goals.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
|
36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
|
Re: My first go-around [Re: PussyFart]
#20258257 - 07/11/14 02:33 PM (10 years, 6 months ago) |
|
|
Personally, if I were you I'd take a clone from your best-looking cluster.
Also make lots of clean prints. That way you don't have to fuck with super dirty wild prints unless you want to.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
|
cowsRmeat
Don't step on the MomeRaths



Registered: 04/23/14
Posts: 3,153
Loc: Wonderland
|
Re: My first go-around [Re: 36fuckin5]
#20258275 - 07/11/14 02:40 PM (10 years, 6 months ago) |
|
|
Okay cool, so basically whatever is available is acceptable, check.
Quote:
36fuckin5 said: Personally, if I were you I'd take a clone from your best-looking cluster.
Also make lots of clean prints. That way you don't have to fuck with super dirty wild prints unless you want to.
Yeah I've got like a half dozen prints off my indoor fruits taken in sterile conditions. Definately done with messing with them dirty wild prints.
-------------------- One day Alice came to a fork in the road and saw a Cheshire cat in a tree.
'Which road do I take?' she asked.
'Where do you want to go?' was his response.
'I don't know', Alice answered.
'Then', said the cat, 'it doesn't matter.'
|
Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
|
Re: My first go-around [Re: cowsRmeat]
#20259011 - 07/11/14 06:06 PM (10 years, 6 months ago) |
|
|
Very nice job, man. I admire your go-getter attitude. We have a bunch of Georgian cultivators who could learn a lot from you.
I agree with 36, that you should keep your clean prints and work exclusively from them. Something worthy of note is that properly taken prints that are well dried and cared for and that are only one generation away from wild are worth a bit more in the marketplace.
|
cowsRmeat
Don't step on the MomeRaths



Registered: 04/23/14
Posts: 3,153
Loc: Wonderland
|
Re: My first go-around [Re: Psilicon]
#20260973 - 07/12/14 12:55 AM (10 years, 6 months ago) |
|
|
Quote:
van der griegen said: Very nice job, man. I admire your go-getter attitude. We have a bunch of Georgian cultivators who could learn a lot from you.
I agree with 36, that you should keep your clean prints and work exclusively from them. Something worthy of note is that properly taken prints that are well dried and cared for and that are only one generation away from wild are worth a bit more in the marketplace.
Thats whats up man. Thanks for that. What do ppl do with near-wild prints I wonder? Cross them into a domestic strain or something? Im really close to access to the marketplace! Only a couple weeks left! I think I've got 5 prints of this strain left.
But yeah, I wasn't trying to brag about some huge success here or anything, I just wanted to share my first small success with the community that helped me get to this point and maybe encourage those that are just starting out and show that anything is possible. I mean, a WILD, practically unsterile, MS print is possible even for us noobs with a lot of reading and a little luck and determination. THANKS TO EVERYONE!
-------------------- One day Alice came to a fork in the road and saw a Cheshire cat in a tree.
'Which road do I take?' she asked.
'Where do you want to go?' was his response.
'I don't know', Alice answered.
'Then', said the cat, 'it doesn't matter.'
|
Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
|
Re: My first go-around [Re: cowsRmeat]
#20262371 - 07/12/14 11:00 AM (10 years, 6 months ago) |
|
|
Yes, some people do cross them with more inbred cultures. But they also have a more complete complement of wild-type alleles and show the most promise for those who wish to pursue their own domestication projects.
TMF and purple mystic are the two most recent and thoroughly documented domestication projects, if you want to do some reading.
|
cowsRmeat
Don't step on the MomeRaths



Registered: 04/23/14
Posts: 3,153
Loc: Wonderland
|
Re: My first go-around [Re: Psilicon]
#20268057 - 07/13/14 06:50 PM (10 years, 6 months ago) |
|
|
Quote:
van der griegen said:
TMF and purple mystic are the two most recent and thoroughly documented domestication projects, if you want to do some reading.
I do indeed, thanks! Off to the search engine
-------------------- One day Alice came to a fork in the road and saw a Cheshire cat in a tree.
'Which road do I take?' she asked.
'Where do you want to go?' was his response.
'I don't know', Alice answered.
'Then', said the cat, 'it doesn't matter.'
|
|