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OfflineFultomte
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First bulk grow, sanity check.
    #20266094 - 07/13/14 09:24 AM (10 years, 6 months ago)

Hi, I made my first grow (BRF) about 10 months ago and now it's time for my second grow.

I have 10 WBS jars 50 cl (~17 oz) filled 75-80% that gives me 375 cl (~127 oz) of grains. The mycelium growth was pretty fast until 50-60% was colonized, then a lot slower. The pictures is from today, 40 days later.

If I understand correctly the halt at 60% is because I used syringe filters?







1. Can I use my jars when it has started to "poop honey drops" as seen in pictures

2. I'm going to use coir, vermiculite and food grade gypsum (Calcium Sulphate). Shall I add some food grade precipiated chalk (Calcium Carbonate) to the mix as well? and how much?

3. Is it too much to use 1 brick of coir (~650g).

4. Any recommended Ratio Grain/Bulk for a first timer?

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OfflineDos Ounce
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Re: First bulk grow, sanity check. [Re: Fultomte]
    #20266113 - 07/13/14 09:31 AM (10 years, 6 months ago)

1.the honey drop poops you speak of are metabolites.

they can show up when the myc gets infected or a little warm. there are multiple things that can cause it, mainly all rooting from some sort of stress.

but it is fine to use your jars, just give em a good whif before you spawn.

2. Not sure about this one

3. 1 brick of coir is fine (you're using verm/gypsum with it I'm assuming). you may not end up using all your mixed substrate since you're using all that birdseed.

4. as far as a ratio goes, 1:2 1:3 1:4 all work fine.
pretty much all I do is pour in all my spawn, usually 6 or 7 quart jars, and then pour in however much substrate it takes to get me to where my substrate level holes are. my subs are 3.5-4.0 inches deep.

Edited by Dos Ounce (07/13/14 10:01 AM)

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InvisibleMudaFuka
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Re: First bulk grow, sanity check. [Re: Dos Ounce]
    #20266178 - 07/13/14 09:56 AM (10 years, 6 months ago)

40 days is a really long time. Did you shake your jars at all during colonisation? I don't know what those filters are do they provide gas exchange?  If it were me I would use 1 brick of coir, 2 quarts of vermiculite and a handful of gypsum with that amount of spawn.


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OfflineFultomte
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Re: First bulk grow, sanity check. [Re: MudaFuka]
    #20266236 - 07/13/14 10:13 AM (10 years, 6 months ago)

"syringe filters with luer lock, 13mm diameter, 0.22µm porosity with PTFE filter"
The part I missed was "Ideal for liquid culture jar lids"

I have bought '9" x 12" KUNIN ECO-FI FELT WHITE 5 SHEETS' for next time. They may have been ready before day 40, but I kind of gave up on them when they stopped at 60% around day 15.

I was going to make new lids today with ECO-FI FELT when I saw that they were fully colonized, so I was caught a little bit of guard =)

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OfflineFultomte
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Re: First bulk grow, sanity check. [Re: Dos Ounce]
    #20266259 - 07/13/14 10:21 AM (10 years, 6 months ago)

Quote:

Dos Ounce said:
1.the honey drop poops you speak of are metabolites.

they can show up when the myc gets infected or a little warm. there are multiple things that can cause it, mainly all rooting from some sort of stress.

but it is fine to use your jars, just give em a good whif before you spawn.

2. Not sure about this one

3. 1 brick of coir is fine (you're using verm/gypsum with it I'm assuming). you may not end up using all your mixed substrate since you're using all that birdseed.

4. as far as a ratio goes, 1:2 1:3 1:4 all work fine.
pretty much all I do is pour in all my spawn, usually 6 or 7 quart jars, and then pour in however much substrate it takes to get me to where my substrate level holes are. my subs are 3.5-4.0 inches deep.




But my jars is closer to 10 pint jars, not quart size... I just can't remember why I bought Calcium Carbonate.

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OfflineDos Ounce
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Re: First bulk grow, sanity check. [Re: Fultomte]
    #20266279 - 07/13/14 10:29 AM (10 years, 6 months ago)

10 pint jars = 5 quart jars.

that's still plenty for you to spawn.

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InvisibleMudaFuka
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Re: First bulk grow, sanity check. [Re: Fultomte]
    #20266281 - 07/13/14 10:30 AM (10 years, 6 months ago)

Five quarts is enough to use with one brick of coir. I generally use 1 brick to 6qts but my spawn to sub ratio is on the high side 1:2 or more. Maybe you got calcium carbonate to buffer PH in peat moss casing layers but I wouldn't add it to your CVG at spawning.


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OfflineDos Ounce
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Re: First bulk grow, sanity check. [Re: MudaFuka]
    #20266283 - 07/13/14 10:30 AM (10 years, 6 months ago)

CVG is tried and true :thumbup:

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Offlinespacechildo
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Re: First bulk grow, sanity check. [Re: MudaFuka]
    #20266297 - 07/13/14 10:34 AM (10 years, 6 months ago)

Am I the only one who sees a mold factory in those grain jars? :stoned2:

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InvisibleMudaFuka
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Re: First bulk grow, sanity check. [Re: spacechildo]
    #20266340 - 07/13/14 10:47 AM (10 years, 6 months ago)

That was my initial thought but I think that may just be sunflower seeds giving it that look. It would not surprise me if they were contaminated though.


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Offlinespacechildo
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Re: First bulk grow, sanity check. [Re: Fultomte]
    #20266365 - 07/13/14 10:55 AM (10 years, 6 months ago)

Quote:

Fultomte said:






no sun flower seeds here, just a scrub pad look. it might be just whispy mycelium
but I think its mold.
add to this that he said colonization halted at 60%, and the weird look on the other jars from a distance view.

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OfflineFultomte
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Re: First bulk grow, sanity check. [Re: spacechildo]
    #20266400 - 07/13/14 11:07 AM (10 years, 6 months ago)

Quote:

spacechildo said:
Quote:

Fultomte said:






no sun flower seeds here, just a scrub pad look. it might be just whispy mycelium
but I think its mold.
add to this that he said colonization halted at 60%, and the weird look on the other jars from a distance view.




Maybe I shall open one and smell it, when I made BRF it smelled just like a bag of common button mushroom. Is it possible to open one and smell it and close the lid again without contaminate the grains?

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Re: First bulk grow, sanity check. [Re: Fultomte]
    #20266407 - 07/13/14 11:09 AM (10 years, 6 months ago)

Shake your jars and see how they recover before spawning them and yah give them a sniff.


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OfflineFultomte
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Re: First bulk grow, sanity check. [Re: MudaFuka]
    #20266457 - 07/13/14 11:20 AM (10 years, 6 months ago)

Quote:

MudaFuka said:
Shake your jars and see how they recover before spawning them and yah give them a sniff.




I just opened jar nr 2 and it didn't smell much at all. Just a faint smell of wet WBS. =(

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Offlinespacechildo
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Re: First bulk grow, sanity check. [Re: MudaFuka]
    #20266469 - 07/13/14 11:23 AM (10 years, 6 months ago)

Do the shake thing, but dont open any grain jar that isnt 100% colonized!
if you open one you introduce mold spores so you have to use that jar as spawn, dont g2g.

so shake and smell thru the filter, see if it recovers healthy in 24-48 hrs!

Goooo Sweden! :super:

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InvisibleMudaFuka
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Re: First bulk grow, sanity check. [Re: Fultomte]
    #20266472 - 07/13/14 11:23 AM (10 years, 6 months ago)

If they were mine I would just spawn them anyway but I lack the proper respect for contams.


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OfflineFultomte
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Re: First bulk grow, sanity check. [Re: MudaFuka]
    #20266503 - 07/13/14 11:32 AM (10 years, 6 months ago)

I guess I can just proceed as normal, I have nothing else to do with my coir and verm anyway and I need to practice pasteurization...

I got my petri-dishes and potato dextrose agar a few days ago so I better start there next time.

But I still think the problem is the Filter.

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Re: First bulk grow, sanity check. [Re: Fultomte]
    #20266529 - 07/13/14 11:38 AM (10 years, 6 months ago)

your lacking gas exchange, get rid of the whatmans those are for lc's not grain spawn


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OfflineFultomte
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Re: First bulk grow, sanity check. [Re: cronicr]
    #20266606 - 07/13/14 11:59 AM (10 years, 6 months ago)

Quote:

cronicr said:
your lacking gas exchange, get rid of the whatmans those are for lc's not grain spawn




Yeah, I found out that too when I just finished inoculating all my jars so I just left them in a dark box in the basement while I was waiting for a 30 day delivery from China with new gear to make G2G and Agar.

But maybe I shall try to spawn them anyway.

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Re: First bulk grow, sanity check. [Re: Fultomte]
    #20266612 - 07/13/14 12:00 PM (10 years, 6 months ago)

yeah those filters suck balls for grains which is probably your issue here


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