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cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
Last seen: 11 minutes, 10 seconds
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Re: Best agar method [Re: Ghatti]
#20260919 - 07/12/14 12:44 AM (10 years, 6 months ago) |
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yeah but the idea from spores is to get more then one isolate so ya sorta want some good sectoring
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Best agar method [Re: cronicr]
#20260922 - 07/12/14 12:45 AM (10 years, 6 months ago) |
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true that!! there will def be enough sectors tho
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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not as much as normal i guess ur right. but it would be hard for me to test that many isolates at once!!
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
Last seen: 11 minutes, 10 seconds
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oh i was just talking about trying to get to an iso in as little plates as possible..thats not my goalQuote:
blindingleaf said: not as much as normal i guess ur right. but it would be hard for me to test that many isolates at once!!
yeah i rarely test them all lol, normally find a good one fairly fast but i'll admit my last two times turned out crap for me which is y i went back to dfa and agar pins
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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I usually start several spore plates at a time just in case one is covered in funk. I'd rather just use one of the others than to try to spend the time cleaning which might cause me not to grab the pieces I want.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Best agar method [Re: cronicr]
#20260936 - 07/12/14 12:48 AM (10 years, 6 months ago) |
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yea cloning way better for me too!
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Best agar method [Re: Ghatti]
#20260945 - 07/12/14 12:50 AM (10 years, 6 months ago) |
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What's dfa? And I've never had my agar pin, how long do they normally take to pin and do you have to let them colonize the entire plate?
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cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
Last seen: 11 minutes, 10 seconds
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Re: Best agar method [Re: Ghatti]
#20260956 - 07/12/14 12:53 AM (10 years, 6 months ago) |
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dogfood agar, tends to pin in under a month no problem if you leave the plate to colonize(any recipe will), you can then drop that pin on anopther plate or just toss it right in a grain jar
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Best agar method [Re: cronicr]
#20260974 - 07/12/14 12:55 AM (10 years, 6 months ago) |
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Ahh gotcha. I tend to always throw my plates in the fridge before they colonize fully.
Do you let them pin just so you know you have a fruiting strain?
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
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Re: Best agar method [Re: cronicr]
#20260985 - 07/12/14 12:57 AM (10 years, 6 months ago) |
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Quote:
cronicr said: i always streak but am yet to get a mono culure that fast mind you i've been doing alot more cloning these days then isolating
Well me too. I actually think you're better off using clones without trying to isolate them any further. Occasionally though I've isolated individual strains for side-by-side experiments.
I was just throwing it out there because Mykes logos mentioned needing 4-5 transfers when you can get one in more like 2-3 transfers. I should probably mention the streaks I make are nearly the circumference of the plate, it has to be long enough.
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Mykes logos
homo nosce te ipsum


Registered: 08/05/12
Posts: 1,108
Loc: FL
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Re: Best agar method [Re: Kizzle]
#20261491 - 07/12/14 04:44 AM (10 years, 6 months ago) |
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Quote:
Kizzle said:
Quote:
cronicr said: i always streak but am yet to get a mono culure that fast mind you i've been doing alot more cloning these days then isolating
Well me too. I actually think you're better off using clones without trying to isolate them any further. Occasionally though I've isolated individual strains for side-by-side experiments.
I was just throwing it out there because Mykes logos mentioned needing 4-5 transfers when you can get one in more like 2-3 transfers. I should probably mention the streaks I make are nearly the circumference of the plate, it has to be long enough.
I rarely ever go past T2 from clone but I assume noobs may transfer larger pieces and may have a tougher time with contaminants... and if you make an isolate from MS than you're much more blind than you would be from clone, and may be wasting your time.
But you can do both... many ways to get it done
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Sockadin



Registered: 01/03/10
Posts: 7,266
Last seen: 1 month, 22 days
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I have stones growing on one of my ATL plates. I was wondering if I can transfer it and revive the plate.
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