Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineTryptx
Stranger

Registered: 04/10/14
Posts: 296
Last seen: 10 years, 2 months
Best agar method
    #20260101 - 07/11/14 09:32 PM (10 years, 6 months ago)

So I have light malt extract and agar.  What's the best ratio.  And what's best for making a mono culture out of; spore, clone,  or lc.  Guessing clone.

Extras: Filter Print Post Top
InvisibleMudaFuka
Poppin bottles
Male User Gallery


Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
OG Cultivator
Re: Best agar method [Re: Tryptx]
    #20260123 - 07/11/14 09:34 PM (10 years, 6 months ago)

I use PDA so I cant really help you with LME ratios but I would say starting with a clone is the fastest way to an isolate.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

Extras: Filter Print Post Top
InvisibleMykes logos
homo nosce te ipsum
 User Gallery

Registered: 08/05/12
Posts: 1,108
Loc: FL Flag
Re: Best agar method [Re: Tryptx]
    #20260142 - 07/11/14 09:38 PM (10 years, 6 months ago)

my fave recipe:

22 grams agar
20 grams ME
1000 mL hot water

1. clones
2. T1
3. T2
4. T3
5. T4

You should have a monoculture by T4 or T5

Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: Best agar method [Re: Mykes logos]
    #20260187 - 07/11/14 09:47 PM (10 years, 6 months ago)

That's a very rich recipe. Go 500ml water, 10g agar, 8-10 grams LME.

Extras: Filter Print Post Top
OfflinecronicrMFacebook
 User Gallery


Registered: 08/07/11
Posts: 62,905
Loc: Van Isle Flag
Last seen: 11 minutes, 10 seconds
Trusted Cultivator
OG Cultivator
Re: Best agar method [Re: Ghatti]
    #20260306 - 07/11/14 10:09 PM (10 years, 6 months ago)

Quote:

Ghatti said:
That's a very rich recipe. Go 500ml water, 10g agar, 8-10 grams LME.



thats like the same recipe cut in half lol


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: Best agar method [Re: cronicr]
    #20260325 - 07/11/14 10:13 PM (10 years, 6 months ago)

Well damned if it ain't. Whoops.

Extras: Filter Print Post Top
InvisibleMudaFuka
Poppin bottles
Male User Gallery


Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
OG Cultivator
Re: Best agar method [Re: Ghatti]
    #20260336 - 07/11/14 10:15 PM (10 years, 6 months ago)

:lmafo:


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

Extras: Filter Print Post Top
OfflineDos Ounce
Fighter of the Nightman
Male User Gallery


Registered: 09/07/12
Posts: 873
Loc: Flag
Last seen: 4 years, 6 months
Re: Best agar method [Re: MudaFuka]
    #20260393 - 07/11/14 10:26 PM (10 years, 6 months ago)

:trololol:  :threadmonitor:

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
Trusted Cultivator
Re: Best agar method [Re: MudaFuka]
    #20260458 - 07/11/14 10:43 PM (10 years, 6 months ago)

Am I the only one who streaks my plates? When you streak the spores across the plate the amount of spores deposited on the agar decreases as you progress down the streak. At the end of streak there are the least spores which means the least amount of strains produced in that area and the least amount of transfers required to get a monoculture. 5 transfers to get an isolate seems kind of excessive.


--------------------

Extras: Filter Print Post Top
Invisiblenucleophile
international cowboy chemist


Registered: 04/28/13
Posts: 299
Re: Best agar method [Re: Kizzle]
    #20260566 - 07/11/14 11:08 PM (10 years, 6 months ago)

Yessir, I also enjoy a good streaked plate, old microbiology habit. Haven't started enough spores on plates to see how well it works for isolating but it's certainly a fine thing for germinating.

Extras: Filter Print Post Top
InvisibleMykes logos
homo nosce te ipsum
 User Gallery

Registered: 08/05/12
Posts: 1,108
Loc: FL Flag
Re: Best agar method [Re: Kizzle]
    #20260579 - 07/11/14 11:14 PM (10 years, 6 months ago)

Quote:

Kizzle said:
Am I the only one who streaks my plates? When you streak the spores across the plate the amount of spores deposited on the agar decreases as you progress down the streak. At the end of streak there are the least spores which means the least amount of strains produced in that area and the least amount of transfers required to get a monoculture. 5 transfers to get an isolate seems kind of excessive.




I prefer to fruit bulk MS, then take clones from the healthiest, best-looking clumps (1st flush). Starting from MS on plates is fine, too tho, yeah... I agree with you.

I always assume newbies need more transfers away from contamination and tend to transfer larger pieces... 5 transfers is definitely too many, but I think they'll find success by then. Assuming they transfer as soon as new growth appears they shouldn't wear the mycelium out.. right?

Extras: Filter Print Post Top
OfflinecronicrMFacebook
 User Gallery


Registered: 08/07/11
Posts: 62,905
Loc: Van Isle Flag
Last seen: 11 minutes, 10 seconds
Trusted Cultivator
OG Cultivator
Re: Best agar method [Re: Mykes logos]
    #20260582 - 07/11/14 11:15 PM (10 years, 6 months ago)

i rarely see sectoring before 4 transfers from spores:shrug:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: Best agar method [Re: cronicr]
    #20260725 - 07/11/14 11:54 PM (10 years, 6 months ago)

What? I usually see sectoring on 1st transfer from my spore plate.

Extras: Filter Print Post Top
OfflinecronicrMFacebook
 User Gallery


Registered: 08/07/11
Posts: 62,905
Loc: Van Isle Flag
Last seen: 11 minutes, 10 seconds
Trusted Cultivator
OG Cultivator
Re: Best agar method [Re: Ghatti]
    #20260730 - 07/11/14 11:56 PM (10 years, 6 months ago)

you may see some sorta sectoring but not real strong sectoring that makes isolating easier


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: Best agar method [Re: cronicr]
    #20260823 - 07/12/14 12:21 AM (10 years, 6 months ago)

I got shit all lucky with my last sleeve and had strong rhizomorphic growth on a spore germination plate. Like at the point of germination and could identify a sector by the time it neared the edge of the plate.(gave me a good place to start anywho)

Made my job a lot shorter.

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
Trusted Cultivator
Re: Best agar method [Re: cronicr]
    #20260836 - 07/12/14 12:23 AM (10 years, 6 months ago)

Quote:

I prefer to fruit bulk MS, then take clones from the healthiest, best-looking clumps (1st flush). Starting from MS on plates is fine, too tho, yeah... I agree with you.

I always assume newbies need more transfers away from contamination and tend to transfer larger pieces... 5 transfers is definitely too many, but I think they'll find success by then. Assuming they transfer as soon as new growth appears they shouldn't wear the mycelium out.. right?



I don't even bother with strain isolation normally and streak mainly for the purpose of making it easier to spot and isolate mycelium from any contaminants in the spores. If I was intent on getting a monoculture though I believe that's the fastest way.

Quote:


i rarely see sectoring before 4 transfers from spores:shrug:




That's basically the point I was trying to make. You can see sectoring on the first plate when you streak.


--------------------

Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: Best agar method [Re: Kizzle]
    #20260866 - 07/12/14 12:31 AM (10 years, 6 months ago)

I've taken to using a bread tie as my inoc loop.

Strip it down to just wire and either touch the very tip to agar, then the outside lightest ring of a print or lay it down and use it that way.

Extras: Filter Print Post Top
OfflinecronicrMFacebook
 User Gallery


Registered: 08/07/11
Posts: 62,905
Loc: Van Isle Flag
Last seen: 11 minutes, 10 seconds
Trusted Cultivator
OG Cultivator
Re: Best agar method [Re: Ghatti]
    #20260877 - 07/12/14 12:33 AM (10 years, 6 months ago)

i always streak but am yet to get a mono culure that fast mind you i've been doing alot more cloning these days then isolating


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Best agar method [Re: cronicr]
    #20260894 - 07/12/14 12:38 AM (10 years, 6 months ago)

i cool loop/blade in receiving plate, and gently, lightly touch/dab the print (instead of swiping/streaking the print)  and swipe one line back in receiving plate.  ill do three lines, 1/2" apart, 1" long about, for same reason kizzle streaks.  def can see sectoring by plate 4
some times even by plate two, u can begin to see where it would begin to sector if left to grow



--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: Best agar method [Re: blindingleaf]
    #20260908 - 07/12/14 12:41 AM (10 years, 6 months ago)

Next time you go from print, try using the tiniest point you can find. Ie: needle point, teeny tiny paperclip, bread tie, etc. It drastically cuts down on the amount of plates you go through.

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Re: mold in agar plate Anonymous 13,428 3 12/04/99 12:35 AM
by Anonymous
* Agar Anonymous 1,558 8 04/15/04 10:15 AM
by Bi0TeK
* several questions about agar majik_monkey 1,844 4 02/10/03 09:26 AM
by fugu
* Fruit on Agar?? Yarry 5,405 17 01/22/04 09:44 PM
by Anonymous
* golden teacher agar asking fugu 3,168 6 01/31/03 12:59 PM
by debianlinux
* Agar to Rice flour substrates foxxybrown 2,194 3 09/01/03 05:00 AM
by eggy
* agar/peroxide conc. iudexk 1,369 1 08/27/01 01:17 PM
by jonnyshaggs420
* agar to agar / g2g degeneration effects ohmatic 2,744 11 01/17/05 03:46 PM
by MushroomFriend

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,671 topic views. 36 members, 106 guests and 33 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.007 seconds on 14 queries.