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OfflinecronicrMFacebook
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Registered: 08/07/11
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Re: Rye grain Brain pain [Re: SpitballJedi] * 1
    #20259879 - 07/11/14 08:50 PM (10 years, 6 months ago)

:feelingfab:


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Rye grain Brain pain [Re: cronicr]
    #20261214 - 07/12/14 01:52 AM (10 years, 6 months ago)

Quote:

I personally wouldn't do it in the bathroom like that. It may be clean, but nowhere near sterile.




Not to fuck with you, but how exactly does one get the place you wash the grime off your body, brush and floss your bacteria ridden mouth, and SHIT into clean? I'd wager the bathroom is the most contaminant ridden room in your entire home, even over the kitchen.

OP, don't inoculate in your bathroom bro.

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OfflinecronicrMFacebook
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Re: Rye grain Brain pain [Re: TheEaglesGift]
    #20261224 - 07/12/14 01:56 AM (10 years, 6 months ago)

nope it's the kitchen by a long shot


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Invisiblenucleophile
international cowboy chemist


Registered: 04/28/13
Posts: 299
Re: Rye grain Brain pain [Re: cronicr]
    #20261238 - 07/12/14 02:03 AM (10 years, 6 months ago)

What's the cleanest room? I guess it doesn't matter that much with a good SAB but usually I do agar work in my little box in the kitchen but I keep my giant one in an outdoor toolshed that's pretty tightly sealed and doesn't have any vents so I'm wondering if maybe it's actually superior.

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OfflinecronicrMFacebook
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Re: Rye grain Brain pain [Re: nucleophile]
    #20261241 - 07/12/14 02:05 AM (10 years, 6 months ago)

the cleanest room will be the one with the least amount of traffic:thumbup: but yep a sab can go anywhere, i park mine in the living room when my soapsbloodsports are on:hehehe:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Invisibleblindingleaf
blue collar underworld
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Registered: 07/19/13
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Re: Rye grain Brain pain [Re: cronicr]
    #20261255 - 07/12/14 02:14 AM (10 years, 6 months ago)

i used my kitchen for a while, cause i used my gas stove as a flame :facepalm:
not the best idea, but goes to show, with good sterile technique and properly built SAB, u can pull a lot off.


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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OfflinecronicrMFacebook
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Re: Rye grain Brain pain [Re: blindingleaf]
    #20261289 - 07/12/14 02:28 AM (10 years, 6 months ago)

oh yeah a sab no problem, i'll post up on the shitter and come out with iso's:laugh2:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Invisibleblindingleaf
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Re: Rye grain Brain pain [Re: cronicr]
    #20261290 - 07/12/14 02:29 AM (10 years, 6 months ago)

:lol:
making most efficient use of ur time!
better than reading!


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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OfflineSockadin
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Re: Rye grain Brain pain [Re: blindingleaf]
    #20261731 - 07/12/14 06:41 AM (10 years, 6 months ago)

Ahh the Toilet Bowl Tek.  I really need to write that one up.

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InvisibleSpitballJedi
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Re: Rye grain Brain pain [Re: TheEaglesGift]
    #20261868 - 07/12/14 08:00 AM (10 years, 6 months ago)

Quote:

TheEaglesGift said:
Quote:

I personally wouldn't do it in the bathroom like that. It may be clean, but nowhere near sterile.




Not to fuck with you, but how exactly does one get the place you wash the grime off your body, brush and floss your bacteria ridden mouth, and SHIT into clean? I'd wager the bathroom is the most contaminant ridden room in your entire home, even over the kitchen.

OP, don't inoculate in your bathroom bro.




I get my bathroom clean by scrubbing the tub, toilet, and sink with a bathroom cleaner. Then, I empty the garbage can and sweep and mop the floor. Then it's clean. Not open air inoculation clean, but normal everyday average person clean. Certainly not sterile, which was my point.


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The Basics
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The Noob Forum
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OfflineDonsanpedro
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Registered: 07/05/14
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Re: Rye grain Brain pain [Re: SpitballJedi]
    #20261946 - 07/12/14 08:42 AM (10 years, 6 months ago)

OP i think it may have sounded rude but i don't think the posts about avoiding the bathroom were meant to be.  I've read a LOT of different people (noobs) saying they inoculated in the bathroom.  It makes sense as its a small room with airflow you can control.  But the back lash from the community is always the same "DON'T inoculate in a b-room blah blah", it can sound harsh but i believe the community just doesn't want to see more people waste their time, money and energy when their entire grow is so easily compromised by their chosen inoculation site (the bathroom).

Let us know what you decided to do, either picked a dif room or got a SAB or what have you. 
DSP

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Offlinematrixmaster
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Registered: 07/11/14
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Re: Rye grain Brain pain [Re: Donsanpedro]
    #20262699 - 07/12/14 12:18 PM (10 years, 6 months ago)

Yeah I live in a studio so there are literally two rooms, my kitchen/bedroom/livingroom and my 5x2 bathroom. I've done three sets of pftek inoculations in the kit/bed/liv that were successful with no SAB. Two contaminated jars out of 50ish. I thought I was being more sterile.

Bought stuff for a SAB and some polyfil. Going to try an salvage these jar somehow. I couldn't find any micropore tape or tyvek.

Also ordered more MS syringes. I'm just going to start over and do it all in a SAB this time with polyfil filters on the lids.

Thanks DSP. I will post if I can figure something out.

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Rye grain Brain pain [Re: matrixmaster]
    #20262949 - 07/12/14 01:35 PM (10 years, 6 months ago)

Did your other 50 jars have gas exchange through the lids?

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Offlinematrixmaster
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Re: Rye grain Brain pain [Re: TheEaglesGift]
    #20263198 - 07/12/14 02:36 PM (10 years, 6 months ago)

None of them did but they were all BRF/Verm for PFtek.  Took between 14-28 days to colonize in TiT at 80 degrees.

My procedure for my boned jar science experiment:

SAB for 5 of 10 jars
Flame to nail and knife then alcohol bath.
Nailed though polyfil into lid removed nail and then hammered knife through the polyfil to open up hole more and stuff in polyfil.
Sometimes I used alcohol on the polyfil rubbing it in my hands, sometimes I sprayed it with disinfectant sometimes I did neither.

This may do nothing to help my colonization. I may grow mold instead. But it's about all the effort I want to put into what will probably be a failure. Even if one of the jars worked I would consider it a win at this point. Seven new syringes on the way so i'm going to study up how to do this the right way in the meantime.

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Rye grain Brain pain [Re: matrixmaster]
    #20263785 - 07/12/14 05:33 PM (10 years, 6 months ago)

Quote:

Seven new syringes on the way so i'm going to study up how to do this the right way in the meantime.




:thumbup:

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Offlinematrixmaster
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Re: Rye grain Brain pain [Re: TheEaglesGift]
    #20271566 - 07/14/14 02:49 PM (10 years, 6 months ago)

Update:

Half of my jars now have spots of mycelium showing up.

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Rye grain Brain pain [Re: matrixmaster]
    #20271685 - 07/14/14 03:21 PM (10 years, 6 months ago)

Post some pictures. There are some good eyes here who do a great job distinguishing mold from mushrooms.

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Offlinematrixmaster
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Registered: 07/11/14
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Last seen: 10 years, 4 months
Re: Rye grain Brain pain [Re: TheEaglesGift]
    #20271989 - 07/14/14 04:21 PM (10 years, 6 months ago)




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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Rye grain Brain pain [Re: matrixmaster]
    #20272060 - 07/14/14 04:34 PM (10 years, 6 months ago)

Looks like the good mycelium. :thumbup:

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Offlinematrixmaster
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Registered: 07/11/14
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Last seen: 10 years, 4 months
Re: Rye grain Brain pain [Re: TheEaglesGift]
    #20272204 - 07/14/14 05:02 PM (10 years, 6 months ago)

Yesssssssss

Thanks for your help TheEaglesGift

Edited by matrixmaster (07/14/14 05:05 PM)

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