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matrixmaster
Stranger
Registered: 07/11/14
Posts: 34
Last seen: 10 years, 4 months
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Rye grain Brain pain
#20257833 - 07/11/14 12:32 PM (10 years, 6 months ago) |
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I've seen a lot of different post on rye grain colonization times. Sometimes I've seen post with mycelium growth after three days and finished jars at 9 days. Other post out there say that jars can take as long as a month to show signs of growth. Quart jars are at day 5 with zero signs of growth. Inoculated with 1.5 cc of MS now incubated at 80 degrees. Should this cause worry?
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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Spores can take weeks to germinate.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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das_shroom
Stranger

Registered: 07/07/14
Posts: 21
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Re: Rye grain Brain pain [Re: 36fuckin5]
#20257884 - 07/11/14 12:40 PM (10 years, 6 months ago) |
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Spores may take weeks to get going...especially if they are MS like you stated in your OP.
My current shortest "first signs of myc" time is 60 hours and my longest is 9 days.
After 14 days with no signs, you might want to start looking at options, but even then, I've heard of 3+ weeks...
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matrixmaster
Stranger
Registered: 07/11/14
Posts: 34
Last seen: 10 years, 4 months
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Re: Rye grain Brain pain [Re: das_shroom]
#20258127 - 07/11/14 01:56 PM (10 years, 6 months ago) |
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Thanks guys. I suppose only time will tell.
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Quote:
day 5 with zero signs of growth
Relax. My current batch of Penis Envy from a 2 year MS spore syringe took between 6-11 days to show growth.
5 days is nothing for MS.
You say they're being incubated. In what? Incubators cause more harm than good as long as your room temp is above 70ish.
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 2 years, 4 months
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Quote:
matrixmaster said: I've seen a lot of different post on rye grain colonization times. Sometimes I've seen post with mycelium growth after three days and finished jars at 9 days. Other post out there say that jars can take as long as a month to show signs of growth. Quart jars are at day 5 with zero signs of growth. Inoculated with 1.5 cc of MS now incubated at 80 degrees. Should this cause worry?
Wait a couple weeks.
PS
--------------------
if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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matrixmaster
Stranger
Registered: 07/11/14
Posts: 34
Last seen: 10 years, 4 months
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Re: Rye grain Brain pain [Re: PrimalSoup]
#20258417 - 07/11/14 03:22 PM (10 years, 6 months ago) |
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TheEaglesGift
they are in a tub in tub with an aquarium heater set to 80 degrees.
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Quote:
matrixmaster said: TheEaglesGift
they are in a tub in tub with an aquarium heater set to 80 degrees.
Why? What is your room temperature? Your room temperature, if not in the extremes, is sufficient. Exposing the colonizing mycelium to a natural day/night light cycle is beneficial. They're not getting that in the dark of the tub I imagine.
I used to raise and breed tropical fresh water fish and and I've had many arguments with people but TIT(tub in tub) incubators are a fire hazard waiting to happen. Those aquarium heaters are less reliable than you'd think and can shatter for no reason, at any time.
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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No pain, no grain. Good luck.
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matrixmaster
Stranger
Registered: 07/11/14
Posts: 34
Last seen: 10 years, 4 months
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Quote:
TheEaglesGift said:
Quote:
matrixmaster said: TheEaglesGift
they are in a tub in tub with an aquarium heater set to 80 degrees.
Why? What is your room temperature? Your room temperature, if not in the extremes, is sufficient. Exposing the colonizing mycelium to a natural day/night light cycle is beneficial. They're not getting that in the dark of the tub I imagine.
I used to raise and breed tropical fresh water fish and and I've had many arguments with people but TIT(tub in tub) incubators are a fire hazard waiting to happen. Those aquarium heaters are less reliable than you'd think and can shatter for no reason, at any time.
Room temps from 69 to 76. Would turning off the aquarium heater in my TiT and leaving the lid off be okay? Would that raise the potential for contams with the jars just being open to the air?
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hushmush

Registered: 12/30/13
Posts: 165
Last seen: 3 years, 8 months
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You can set the jars on the shelf or something. They don't need a TiT with your room temps. Assuming you have some filter on the lids you will be fine. The tit doesn't help protect them from contaims.
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matrixmaster
Stranger
Registered: 07/11/14
Posts: 34
Last seen: 10 years, 4 months
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Re: Rye grain Brain pain [Re: hushmush]
#20259341 - 07/11/14 07:22 PM (10 years, 6 months ago) |
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No filters I just have lids on the jars.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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There is a good chance they will stall without a filtered GE hole in the lid, unless you are allowing for GE in some other way.
-------------------- The Basics
A little civility goes a long way
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matrixmaster
Stranger
Registered: 07/11/14
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Last seen: 10 years, 4 months
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Ideas for a way to create GE at this point?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,867
Loc: ★
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At this point you're boned dude. If the lids are literally sealed there isn't a way to open/disrupt them unless you think you can fit a filter on each lid while keeping everything sterile. Possible perhaps, but insanely difficult.
At this rate they're almost guaranteed to stall.
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matrixmaster
Stranger
Registered: 07/11/14
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Last seen: 10 years, 4 months
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Re: Rye grain Brain pain [Re: Inocuole]
#20259533 - 07/11/14 07:50 PM (10 years, 6 months ago) |
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Nooooooooooo. That's a real bummer. I'll crack the jars put them back in the incubator on a light cycle and try to keep it sterile I suppose.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,867
Loc: ★
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I thought we discussed the incubator being bad already? Putting jars on a light cycle after opening them will have no positive effects. That's like leaving a raw steak in the sun with the hopes of it cooking before it rots.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Rye grain Brain pain [Re: Inocuole]
#20259552 - 07/11/14 07:52 PM (10 years, 6 months ago) |
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If you have a SAB or flowhood, you can try cracking them open 2-3 times a day. Don't take the lid completely off, just crack it so some gas will escape. You won't be trying to get air in, just some gas out for a few seconds and then tighten the lid back on.
I know some people use plastic lids with no GE hole. They just leave the lid ever so slightly cracked to vent. But, I would be afraid to do that with a 2-piece metal lid.
Lids
-------------------- The Basics
A little civility goes a long way
The Noob Forum
The Hammock Hangers' Forum
Edited by SpitballJedi (07/11/14 07:53 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: Rye grain Brain pain [Re: Inocuole]
#20259568 - 07/11/14 07:55 PM (10 years, 6 months ago) |
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You could plausibly have a 15-20% success rate doing this but the chances are greater that you will waste time. In a hobby where patience is important, it's pure torture to set yourself back to the beginning, so try to do as little dwelling on it as possible if you have a choice. If there's no chance in hell of you being able to start over right now go ahead and try what Spitball mentioned. Otherwise, learning experience.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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Quote:
SpitballJedi said: If you have a SAB or flowhood, you can try cracking them open 2-3 times a day. Don't take the lid completely off, just crack it so some gas will escape. You won't be trying to get air in, just some gas out for a few seconds and then tighten the lid back on.
2-3 times a day would be way too much. Once every couple days would do it. But this is a recipe for failure.
Quote:
I know some people use plastic lids with no GE hole. They just leave the lid ever so slightly cracked to vent.
Those people probably get a shitload of contams they don't talk about.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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