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InvisibleGhatti
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culture opinion please
    #20257134 - 07/11/14 09:12 AM (10 years, 6 months ago)

Will someone please take a look at this plate and tell me if this is tomatose myc or what?
It behaves like mycelium as far as how fast it has grown. It presumably came from a clean plate as well but I've been having all sorts of contam issues lately so I'd like another set of eyes.


Edited by Ghatti (07/11/14 10:25 AM)

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InvisibleGhatti
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Re: culture opinion please [Re: Ghatti]
    #20258148 - 07/11/14 02:03 PM (10 years, 6 months ago)

This plate is growing almost perfect radially like mold would. I doubt its an isolates as my other plates still have 2-4 sectors on them.

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Invisible36fuckin5
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Re: culture opinion please [Re: Ghatti]
    #20258197 - 07/11/14 02:17 PM (10 years, 6 months ago)

Looks fine to me, but my eyes suck.

It's tomentose, not tomatose.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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InvisibleGhatti
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Re: culture opinion please [Re: 36fuckin5]
    #20258205 - 07/11/14 02:18 PM (10 years, 6 months ago)

Tomatoes.

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InvisibleGhatti
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Re: culture opinion please [Re: Ghatti]
    #20258210 - 07/11/14 02:20 PM (10 years, 6 months ago)

How can you determine sectors on a plate like this? My other plates still have sectors so I'm assuming this one does too.

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InvisibleTheEaglesGift
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Re: culture opinion please [Re: Ghatti]
    #20258241 - 07/11/14 02:30 PM (10 years, 6 months ago)

That's not mold, but does look odd due to it being strongly tomentose growth.

I have no idea how to go about identifying sectors in that(if there even are any), and considering how tomentose it is I'm not sure why you'd want to.

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OfflinePussyFart
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Re: culture opinion please [Re: TheEaglesGift]
    #20258262 - 07/11/14 02:35 PM (10 years, 6 months ago)

Take a sample to a new dish with less nutrients and I bet you'll see some rhizomorphic growth.

Mycellium will often go from rhizomorphic to cottony and back based on the food source.

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Invisible36fuckin5
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Re: culture opinion please [Re: PussyFart]
    #20258264 - 07/11/14 02:36 PM (10 years, 6 months ago)

I don't see any sectors on that plate. Looks like you have a monoculture. Good job. Time to test it now.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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InvisibleGhatti
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Re: culture opinion please [Re: 36fuckin5]
    #20258279 - 07/11/14 02:42 PM (10 years, 6 months ago)

So you can see sectors with cottony mycelium? And PF is correct, this was taken from a highly rhizomorphic sector on less nutritious agar.

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Invisibleblindingleaf
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Re: culture opinion please [Re: Ghatti]
    #20258322 - 07/11/14 02:52 PM (10 years, 6 months ago)

i don't think thats an isolate


--------------------
A few thoughts on cultivation
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Invisibleblindingleaf
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Re: culture opinion please [Re: blindingleaf]
    #20258324 - 07/11/14 02:52 PM (10 years, 6 months ago)

or if it is, its not a cubensis isolate


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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InvisibleGhatti
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Re: culture opinion please [Re: blindingleaf]
    #20258338 - 07/11/14 02:57 PM (10 years, 6 months ago)

It is indeed a cube.

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Invisibleblindingleaf
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Re: culture opinion please [Re: Ghatti]
    #20258341 - 07/11/14 02:58 PM (10 years, 6 months ago)

word.  just looks weird to me.  i'd do what PF said, and see what it looks like.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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Invisibleblindingleaf
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Re: culture opinion please [Re: blindingleaf]
    #20258432 - 07/11/14 03:26 PM (10 years, 6 months ago)

so i could be totally wrong here, but what i mean when i say it looks weird is that there is that white stuff on top, but its not reaching out to the edge.  wispiness/tomentose/linear whatever u wanna call it is normal, be it from too many strain not differentiating/sectoring yet, early on in a culture, or just the nature of that particular set of genetics.  this is a plate 2nd transfer from spore (so third plate) with wispiness, but no solid white on top of it.



contaminates usually grow outside leading edge, or right at it, so thats why its weird to me, cause thats not happening with urs, its just sitting on top.
I've been wrong a million times tho, just throwing it out there


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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InvisibleTheEaglesGift
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Re: culture opinion please [Re: PussyFart]
    #20258488 - 07/11/14 03:41 PM (10 years, 6 months ago)

Quote:

Mycellium will often go from rhizomorphic to cottony and back based on the food source.




Why does it do that?

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OfflinePussyFart
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Re: culture opinion please [Re: TheEaglesGift]
    #20258496 - 07/11/14 03:43 PM (10 years, 6 months ago)

Quote:

TheEaglesGift said:
Quote:

Mycellium will often go from rhizomorphic to cottony and back based on the food source.




Why does it do that?



It goes rhizomorphic reaching out for food.

On a highly nutritious substrate it tends to grow slower(taking it's sweet ass time eating), and look more like that(cottony) or tomentose.

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Invisible36fuckin5
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Re: culture opinion please [Re: PussyFart]
    #20259074 - 07/11/14 06:23 PM (10 years, 6 months ago)

Quote:

PussyFart said:
Quote:

TheEaglesGift said:
Quote:

Mycellium will often go from rhizomorphic to cottony and back based on the food source.




Why does it do that?



It goes rhizomorphic reaching out for food.

On a highly nutritious substrate it tends to grow slower(taking it's sweet ass time eating), and look more like that(cottony) or tomentose.




:whathesaid:

This is why we don't put much nutrition in our agar unless it's for long-term storage. The less stuff to eat in there (to a point) the faster they're gonna grow to look for more.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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InvisibleGhatti
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Re: culture opinion please [Re: 36fuckin5]
    #20259204 - 07/11/14 06:50 PM (10 years, 6 months ago)

Yeah the culture went from half strength agar to full (about 8.5g LME) per 500ml and most of my plates are now like this and I'm none too happy about it.

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Invisible36fuckin5
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Re: culture opinion please [Re: Ghatti]
    #20259225 - 07/11/14 06:54 PM (10 years, 6 months ago)

Why? It doesn't make a difference except in how it looks.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

Extras: Filter Print Post Top
InvisibleGhatti
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Registered: 06/23/14
Posts: 1,733
Re: culture opinion please [Re: 36fuckin5]
    #20259276 - 07/11/14 07:07 PM (10 years, 6 months ago)

Because apparently I can't tell an isolate on tomentose myc.

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Invisible36fuckin5
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Re: culture opinion please [Re: Ghatti]
    #20259286 - 07/11/14 07:11 PM (10 years, 6 months ago)

Sometimes it's more obvious on the bottom of the plate, or if you shine a light through it.

Or maybe you just got monocultures.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Re: culture opinion please [Re: 36fuckin5]
    #20259394 - 07/11/14 07:29 PM (10 years, 6 months ago)

It's definitely easier to see sectoring with a little less nutrients.

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Shop: Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   PhytoExtractum Kratom Powder for Sale   North Spore Bulk Substrate   Mushroom-Hut Mono Tub Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order


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