Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinedr.alkaline
 User Gallery


Registered: 12/15/12
Posts: 684
Last seen: 5 years, 9 months
Do you need a monoculture before inoculating grain?
    #20254721 - 07/10/14 07:19 PM (10 years, 6 months ago)

So here is the skinny:

I inoc'd an agar dish with spores from a syringe and the culture is growing very well The growth is clean buy very cottony and I cant spot any sectors, so I wonder if I should go through 2 or 3 more transfers to try and isolate a single strain before inoculating a grain petri as per the "Violet Tek", or can I inoculate the grain petri with my cottony growth from the initial plate?

http://www.shroomery.org/forums/showflat.php/Number/19035259

Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
Re: Do you need a monoculture before inoculating grain? [Re: dr.alkaline]
    #20254775 - 07/10/14 07:25 PM (10 years, 6 months ago)

grain in a petri dish or grain water agar? :confused2:

but no, you dont need to have an isolate to inoc anything, clean growth is perfect.

If you want a consistent culture you'd need to isolate but that's no the Q I guess..

Extras: Filter Print Post Top
Offlinedr.alkaline
 User Gallery


Registered: 12/15/12
Posts: 684
Last seen: 5 years, 9 months
Re: Do you need a monoculture before inoculating grain? [Re: spacechildo]
    #20254789 - 07/10/14 07:29 PM (10 years, 6 months ago)

spores were placed on MEA, then I am going to put the resulting growth on some brown rice  ziploc twist n lock contraptions as described in the link I posted. For some reason I was thinking it would be best to do a transfer to try and get sectors and then inoc with the fastest growing sector, but you are saying as long as it is clean I can take a slice of my fluff and inoc the grain with that and it should be a okay? Im not trying to grow a trophy shroom if that helps, just want some decent fruit.

EDIT: I just realized that when you inoc BRF cake you are using a multispore suspension, so I guess the fluff is just fine for what I am doing. I am a moron.

Edited by dr.alkaline (07/10/14 07:35 PM)

Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
Re: Do you need a monoculture before inoculating grain? [Re: spacechildo]
    #20254824 - 07/10/14 07:35 PM (10 years, 6 months ago)

then just transfer healthy growth.
even when you see sectoring and you choose the fastest growing one,
even that is most likely not yet an isolate.
AND the isolate you end up with might not even fruit at all or just very poorly.

I like the variety MS grows give, and I like to do some extra transfers
just to feel sure its as clean as I'll get it.

Extras: Filter Print Post Top
Offlinedr.alkaline
 User Gallery


Registered: 12/15/12
Posts: 684
Last seen: 5 years, 9 months
Re: Do you need a monoculture before inoculating grain? [Re: spacechildo]
    #20254838 - 07/10/14 07:37 PM (10 years, 6 months ago)

Thanks that was really helpful :cool:

whats MS? multiple sclerosis? multispore?

Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
Re: Do you need a monoculture before inoculating grain? [Re: dr.alkaline]
    #20254871 - 07/10/14 07:43 PM (10 years, 6 months ago)

:rofl:

multispore, yes.

Stro's agar thread explains everything about growing with agar with lots of good pics!

Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: Do you need a monoculture before inoculating grain? [Re: dr.alkaline]
    #20254902 - 07/10/14 07:47 PM (10 years, 6 months ago)

You don't have to have a monoculture to transfer to grains. I always make at least one transfer to another agar plate to make sure the culture is clean though.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum

Extras: Filter Print Post Top
Offlinedr.alkaline
 User Gallery


Registered: 12/15/12
Posts: 684
Last seen: 5 years, 9 months
Re: Do you need a monoculture before inoculating grain? [Re: spacechildo]
    #20254922 - 07/10/14 07:51 PM (10 years, 6 months ago)

That thread got me started in agar, I am very appreciative of it. The information is great if anyone else is looking to get into the agar.

Extras: Filter Print Post Top
Offlinedr.alkaline
 User Gallery


Registered: 12/15/12
Posts: 684
Last seen: 5 years, 9 months
Re: Do you need a monoculture before inoculating grain? [Re: SpitballJedi]
    #20255140 - 07/10/14 08:28 PM (10 years, 6 months ago)

Quote:

SpitballJedi said:
You don't have to have a monoculture to transfer to grains. I always make at least one transfer to another agar plate to make sure the culture is clean though.




Is it difficult to visibly see that it is clean on the first plate?

Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: Do you need a monoculture before inoculating grain? [Re: dr.alkaline]
    #20255251 - 07/10/14 08:47 PM (10 years, 6 months ago)

sometimes. If you have good rhyzomorphic mycelium, it's easier because the contams will have a different texture, often cottony or something.

It can be more difficult with cottony or fluffy myc because a lot of things look like that. But rhyzomorphs are fairly distinct.

I can't always spot a contam when everything is all white and fluffy, but even then you can sometimes see slight color and texture differences.

I usually make a plate and take 5-6 transfers from it to new plates. Generally, I will make a 2-3 transfers from each of those.

Then, after those transfers are made, if I can't see a contam, go ahead noc up some grains with what's left in the dish. Or just seal them up in zip-locks and stick them in the culture fridge for later.

The more transfers you make, the more you will start to see sectors in the myc. This is very distinguished and contams become much easier to see.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum

Extras: Filter Print Post Top
InvisibleViolet
 User Gallery


Registered: 12/06/11
Posts: 4,205
Re: Do you need a monoculture before inoculating grain? [Re: dr.alkaline]
    #20255580 - 07/10/14 09:58 PM (10 years, 6 months ago)

Quote:

dr.alkaline said:
So here is the skinny:

I inoc'd an agar dish with spores from a syringe and the culture is growing very well The growth is clean buy very cottony and I cant spot any sectors, so I wonder if I should go through 2 or 3 more transfers to try and isolate a single strain before inoculating a grain petri as per the "Violet Tek", or can I inoculate the grain petri with my cottony growth from the initial plate?

http://www.shroomery.org/forums/showflat.php/Number/19035259




I transfer to "grain petri" right from the spore germ dish.
I don't bother to isolate until after a clone selection has been taken from that dish. It involves far fewer transfers, and far fewer lesser cultures.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts

Extras: Filter Print Post Top
Offlinedr.alkaline
 User Gallery


Registered: 12/15/12
Posts: 684
Last seen: 5 years, 9 months
Re: Do you need a monoculture before inoculating grain? [Re: Violet]
    #20255944 - 07/10/14 11:31 PM (10 years, 6 months ago)

Quote:

SpitballJedi said:
sometimes. If you have good rhyzomorphic mycelium, it's easier because the contams will have a different texture, often cottony or something.

It can be more difficult with cottony or fluffy myc because a lot of things look like that. But rhyzomorphs are fairly distinct.

I can't always spot a contam when everything is all white and fluffy, but even then you can sometimes see slight color and texture differences.

I usually make a plate and take 5-6 transfers from it to new plates. Generally, I will make a 2-3 transfers from each of those.

Then, after those transfers are made, if I can't see a contam, go ahead noc up some grains with what's left in the dish. Or just seal them up in zip-locks and stick them in the culture fridge for later.

The more transfers you make, the more you will start to see sectors in the myc. This is very distinguished and contams become much easier to see.




Good advice, I think I will follow this in the future. Bad news is I inocd my "grain petris" with the first spore germ plate before seeing this post. Visually, the spore germs plates look clean as a whistle and if I have a contam it will probably be obvious as colonization proceeds. I guess we will have to see :shrug:

Quote:

Violet said:


I transfer to "grain petri" right from the spore germ dish.
I don't bother to isolate until after a clone selection has been taken from that dish. It involves far fewer transfers, and far fewer lesser cultures.




Thanks Violet, as you can already tell I am finally giving the Violet Tek a go. If my progress looks good I will update the mother thread with images and details. Thanks for all your help.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Can one do grain to grain / mycelium transfers via oven tek? none 1,539 4 08/04/03 03:02 PM
by tripndicular
* Spawn to inoculate grain? lumpus 786 1 05/10/03 11:10 AM
by MykeOfile
* PC'ing grains and transfering to jars BigJohnson 1,815 11 04/09/03 11:06 AM
by tripndicular
* Grain to Grain kindkesey 2,359 13 03/03/04 07:50 PM
by Anonymous
* transferring mycelium wikedanjel 4,251 13 01/10/03 03:54 AM
by debianlinux
* pinheads on petri dish Senovio 1,783 7 07/27/03 04:53 PM
by Sev
* Liquidplaya's 99% contam free grain -> X TransfTek liquidplaya 2,155 7 10/25/16 10:59 AM
by Greg
* G2G transfers
( 1 2 all )
kjb1891 33,625 20 12/19/18 11:45 AM
by Nichrome

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
954 topic views. 35 members, 122 guests and 36 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.007 seconds on 12 queries.