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OfflineEdmunter
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Tyvek sleeves tips
    #20247226 - 07/09/14 11:15 AM (10 years, 6 months ago)

How do you sterilise and store tyvek sleeves after use?

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Invisiblemushmagic
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Re: Tyvek sleeves tips [Re: Edmunter]
    #20247286 - 07/09/14 11:32 AM (10 years, 6 months ago)

You wont be able to sterilize unless you run it through a pc which would be rendered pointless as soon as you put them on. You can sanitize it though by simply wiping them down with iso.

I personally don't even bother with sleeves though, I just use latex gloves and wipe my arms with iso.


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OfflineMdahmer
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Re: Tyvek sleeves tips [Re: mushmagic]
    #20247316 - 07/09/14 11:41 AM (10 years, 6 months ago)

I've heard of people usng them for a long tme w/o sterilizing but I've also seen a little picture of a guy sterilizing his Ina. Qt jar with his PC run. It couldn't hurt to cleanse them every once in a while

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OfflineEdmunter
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Re: Tyvek sleeves tips [Re: mushmagic]
    #20247341 - 07/09/14 11:48 AM (10 years, 6 months ago)

Do you wear clean clothes and shower beforehand?  Im getting quite good results but u can never be too clean with this skill.

Thanks for the info

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Invisiblemushmagic
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Re: Tyvek sleeves tips [Re: Edmunter]
    #20247428 - 07/09/14 12:07 PM (10 years, 6 months ago)

Shower and freshly laundered clothes is definitely a good idea. I personally don't find it completely necessary if your sterile technique is up to par although I would still definitely recommend it.


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OfflineMdahmer
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Re: Tyvek sleeves tips [Re: Edmunter]
    #20247453 - 07/09/14 12:11 PM (10 years, 6 months ago)

I try to but I do cheat sometimes, i always take my shirt off and I like to come out of the shower and just work in my boxers but if I don't shower I use mouthwash and rub my arms and stuff with alcohol before putting sleeves on

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OfflineEdmunter
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Re: Tyvek sleeves tips [Re: Mdahmer]
    #20247756 - 07/09/14 01:19 PM (10 years, 6 months ago)

But never just boxers and sleeves and a mask.....Thats just wrong......

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OfflineMdahmer
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Re: Tyvek sleeves tips [Re: Edmunter]
    #20247762 - 07/09/14 01:22 PM (10 years, 6 months ago)

Of course it's wrong...you need gloves bro, no gloves so sucky sucky transfer time

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OfflinePussyFart
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Re: Tyvek sleeves tips [Re: Edmunter]
    #20247831 - 07/09/14 01:42 PM (10 years, 6 months ago)

Quote:

Edmunter said:
How do you sterilise and store tyvek sleeves after use?



I don't...

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OfflineEdmunter
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Re: Tyvek sleeves tips [Re: PussyFart]
    #20248012 - 07/09/14 02:15 PM (10 years, 6 months ago)

Quote:

PussyFart said:
Quote:

Edmunter said:
How do you sterilise and store tyvek sleeves after use?



I don't...





So just putting them away and sanitising is fine.  I should imagine slightly damp is better as contams will stick to that in theory.

Whats so special about tyvek anyhow?

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Re: Tyvek sleeves tips [Re: Edmunter]
    #20248026 - 07/09/14 02:16 PM (10 years, 6 months ago)

remember what the point of the sleeves is, it's to stop the gross shit falling from your body getting on your work, wipe them down with alcohol and get to work


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Re: Tyvek sleeves tips [Re: cronicr]
    #20248098 - 07/09/14 02:28 PM (10 years, 6 months ago)

Good point.  For this particular session I didnt need to worry too much as I was making the first transfer from 2 clone plates.  One had trich and the other bacteria.  Im really just practising cleaning them up and isolating.  I took 3 from each and the 2 or 3 fastest growing cultures I will use to make isolates and the grow them out.  Does this sound right?

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Re: Tyvek sleeves tips [Re: Edmunter]
    #20248103 - 07/09/14 02:29 PM (10 years, 6 months ago)

:firstladyofapproval:


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OfflineRogerRabbitM
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Re: Tyvek sleeves tips [Re: cronicr]
    #20248437 - 07/09/14 03:29 PM (10 years, 6 months ago)

Tyvek sleeves can be washed in the machine and dried in the dryer.  Then simply bag them up to keep dust off until used.  They don't need to be sterile because they never come in direct contact with sterile media.
RR


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