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InvisibleMushiez
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Registered: 04/28/14
Posts: 1,057
Cloning the Albno PE chode
    #20247948 - 07/09/14 02:04 PM (10 years, 6 months ago)

Hey everyone, I just wanted to share my experience with cloning some APE on agar. I'll take you from harvest to culture, hope you all enjoy.

Transfers where done with the help of a Nauire LF bench





Printing


I learned my lesson to 1) not cut a fruit, and 2) take smaller pieces. but I still managed


Myc still not as thick as I want on 2nd transfer though the plate is clean


I took a small rizo piece looking similar to the one on the just above the top left transfer here


and here it is today, and I am well pleased :smile2:


Now THIS is the dense myc I was after!


I think it's a monoculture, and if there is any more sectoring potential someone let me know.

I can't open this petri dish, do transfers, or make a master until I've built my laminar flow hood. I'm not gonna let myself potentially contam this even using a SAB

Should I let it cover the plate? I'd like to make a master slant of it one day...

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