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InvisibleAbshroom
Cause he can


Registered: 06/05/14
Posts: 810
Loc: Germany Flag
LC to LC, like g2g?
    #20241808 - 07/08/14 12:09 PM (10 years, 6 months ago)

Hey guys,
would it be possible/useful to do a LC to LC?
If you made sure that your LC is free of contamn (inoculate several test jars), would it be recommend to inoculate another LC jar with the first LC to increase the amount of it, similar like doing g2g on grain jars?
Please no flame about LC, I know it's very risky, you can't detect contamn in the LC directly, etc etc etc.
Just want to know if my thought is correct/useful :wink:

Greetz
Abshroom

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Invisibletoxetel
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Registered: 08/13/12
Posts: 708
Re: LC to LC, like g2g? [Re: Abshroom]
    #20242001 - 07/08/14 12:47 PM (10 years, 6 months ago)

It sounds like you already know the answer.

It's of course possible to inoculate a new LC with an old LC. But of course it's not recommended -- very few cultivators recommend using liquid cultures at all, for the reasons you refer to.

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OfflinecronicrMFacebook
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Registered: 08/07/11
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Re: LC to LC, like g2g? [Re: toxetel]
    #20242068 - 07/08/14 01:01 PM (10 years, 6 months ago)

if your jars come out clean just g2g2


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InvisibleAbshroom
Cause he can


Registered: 06/05/14
Posts: 810
Loc: Germany Flag
Re: LC to LC, like g2g? [Re: cronicr]
    #20242133 - 07/08/14 01:14 PM (10 years, 6 months ago)

Quote:

toxetel said:
It sounds like you already know the answer.

It's of course possible to inoculate a new LC with an old LC. But of course it's not recommended -- very few cultivators recommend using liquid cultures at all, for the reasons you refer to.




Thought so, but after checking for contamination through test jars it would be a good choice to increase the LC through inoculate another LC if you would want to stay on using LC?

Quote:

cronicr said:
if your jars come out clean just g2g2




Does g2g works faster than using LC?
And another short question:
If I use a fully colonized grain jar for g2g on grain jars what should I do with the master grain jar if I don't use the whole master jar immediately for g2g. Could I just put it in the refrigerator or should I just let it stay by the other jars? Wouldn't the master jar get worse when letting it colonize to long?

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Invisibletoxetel
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Registered: 08/13/12
Posts: 708
Re: LC to LC, like g2g? [Re: Abshroom]
    #20242268 - 07/08/14 01:37 PM (10 years, 6 months ago)

Quote:

Abshroom said:
Thought so, but after checking for contamination through test jars it would be a good choice to increase the LC through inoculate another LC if you would want to stay on using LC?




Test jars aren't a foolproof way to test liquid cultures. Contaminants can coexist practically undetectably with cubensis mycelium. That means a jar can appear to be 100% colonized by cubensis mycelium but really harbor a hidden contamination. Also, it's possible for a contaminant in an LC to fail to colonize your test jars but succeed in contaminating later jars.

You already have your answer -- it is not recommended. So it's not a good choice. You don't have to accept this answer, but arguing against it isn't going to get anywhere. If you disagree with my advice, go ahead and give your LC-to-LC idea a shot, and I'll wish you luck.

Quote:

Does g2g works faster than using LC?




Yes, typically. With LC, you can only use so much inoculant before you upset the moisture level in the jar. With G2G, you can use as much inoculant grain as you can fit in the jar. This makes for faster colonization.

Quote:

If I use a fully colonized grain jar for g2g on grain jars what should I do with the master grain jar if I don't use the whole master jar immediately for g2g. Could I just put it in the refrigerator or should I just let it stay by the other jars? Wouldn't the master jar get worse when letting it colonize to long?




You should use the whole master jar if you can. If you can't, you could spawn the remainder to a small bulk grow, I guess. In principle you can store it in the fridge but it's not recommended -- opening and closing the jar repeatedly could compromise the aseptic environment inside the jar.

Why wouldn't you use the whole jar? Unless you have a tiny pressure cooker, you're going to be sterilizing 7 to 10 jars at a time anyway, right?

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InvisibleAbshroom
Cause he can


Registered: 06/05/14
Posts: 810
Loc: Germany Flag
Re: LC to LC, like g2g? [Re: toxetel]
    #20242289 - 07/08/14 01:42 PM (10 years, 6 months ago)

Okay thanks for the advice :wink:
And about the pc, the maximum I can sterilize at once are 4 pint jars :frown: but of course I could pc 2 times 4 jars a day so I could g2g a master jar to 8 grain jars :wink:

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Invisibletoxetel
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Registered: 08/13/12
Posts: 708
Re: LC to LC, like g2g? [Re: Abshroom]
    #20242396 - 07/08/14 02:01 PM (10 years, 6 months ago)

Ah, sounds like you've got a little baby pressure cooker. Make sure to check the pressure it operates at -- a lot of smaller pressure cookers run at around 12 psi, but most grain prep teks expect you to be using a 15 psi PC. You may need to extend your cooking time.

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InvisibleAbshroom
Cause he can


Registered: 06/05/14
Posts: 810
Loc: Germany Flag
Re: LC to LC, like g2g? [Re: toxetel]
    #20242420 - 07/08/14 02:05 PM (10 years, 6 months ago)

Yeah it's really tiny... hate it so much, but currently can't find a bigger one. One the amazon website from the United States I find many 22 quart pc but on the german one (I live in germany) the biggest one I found was 10 litres (about nearly 10 quart)
Currently I sterilize my (pint, don't have any bigger ones) grain jars for 90 minutes (like RR is telling in his videos), should be enough, shouldn't it?

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OfflineMdahmer
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Registered: 04/05/14
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Re: LC to LC, like g2g? [Re: Abshroom]
    #20242600 - 07/08/14 02:47 PM (10 years, 6 months ago)

Your PC probably only goes to 12psi, I cannot say for sure but like toxetel said smaller ones usually are 12, I have a tall 8qt that I can fit 3qt jars in, it goes to 12 so I do 2 hours sometimes a few extra minutes for fun it to be safe

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Invisibletoxetel
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Registered: 08/13/12
Posts: 708
Re: LC to LC, like g2g? [Re: Mdahmer]
    #20242915 - 07/08/14 03:55 PM (10 years, 6 months ago)

90 minutes at 12 psi is probably okay for pint jars, but I'd do 120 minutes just to be safe.

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