Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   MagicBag.co All-In-One Bags That Don't Suck   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisibled0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
Too good to be true - all 3 agar contamed (update)
    #20239499 - 07/07/14 09:06 PM (10 years, 6 months ago)

Well I was all excited to take 3 transfers from my 3 types of myc on agar today to clean them up and I see that they all contamed. They look pretty good until today I notice it. I managed to get either two or three different types of contams too. :rolleyes:

Oh well more time to clean up my sterile technique I suppose. At least one, possibly two were wild prints.

One was a very white spot, but it looked too perfect and not whispy enough for pan myc so I did have my suspicions on that on. Today it turned yellow.

So is it better to transfer early on before these contaminates sporulate? I think that is what gave them their color which is why I didn't see them.

I knew it was too good to be true I shouldn't have gotten so excited.

I think I should stick with growing salvia instead :lol:


--------------------

Edited by d0urd3n (07/08/14 06:22 PM)

Extras: Filter Print Post Top
InvisibleMushiez
 User Gallery


Registered: 04/28/14
Posts: 1,057
Re: Too good to be true - all 3 agar contamed [Re: d0urd3n]
    #20239521 - 07/07/14 09:11 PM (10 years, 6 months ago)

transfer as far away from the unsporulated as you can

Extras: Filter Print Post Top
OfflineBLINKfan420
Jedi
I'm a teapot


Registered: 07/06/12
Posts: 1,078
Last seen: 5 months, 6 days
Re: Too good to be true - all 3 agar contamed [Re: d0urd3n]
    #20239534 - 07/07/14 09:13 PM (10 years, 6 months ago)

Did you get any clean mushie myc growth at all?? If so and..

Assuming you have more agar and dishes I'd say cut the best looking wedges and place them in new dishes.  Keep isolating it and it will clean up.  Unless your contams are from you're instruments (inoculation loop, dirty petri dishes, etc.) 

Im no expert so wait for one of the pros to comment but I'd say dont give up!!

BLINK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.

Extras: Filter Print Post Top
OfflineCaptainpaps
Babaji NA MA AUM
Male User Gallery


Registered: 11/24/13
Posts: 1,533
Last seen: 8 years, 5 months
Re: Too good to be true - all 3 agar contamed [Re: BLINKfan420]
    #20239543 - 07/07/14 09:15 PM (10 years, 6 months ago)

I haven't had any luck with wild prints and agar yet... i always get cobweb and its a bitch finding a good area to transfer


--------------------




Extras: Filter Print Post Top
Invisibled0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
Re: Too good to be true - all 3 agar contamed [Re: Captainpaps]
    #20239801 - 07/07/14 10:38 PM (10 years, 6 months ago)

Oh I thought that if they were contained I had to start over because it would spread the spores around my workplace when I opened the agar up?


--------------------

Extras: Filter Print Post Top
OfflineCaptainpaps
Babaji NA MA AUM
Male User Gallery


Registered: 11/24/13
Posts: 1,533
Last seen: 8 years, 5 months
Re: Too good to be true - all 3 agar contamed [Re: d0urd3n]
    #20239839 - 07/07/14 10:48 PM (10 years, 6 months ago)

if your able to get a clean spot to transfer from I'd say go for it, and just keep isolating


--------------------




Extras: Filter Print Post Top
OfflineBLINKfan420
Jedi
I'm a teapot


Registered: 07/06/12
Posts: 1,078
Last seen: 5 months, 6 days
Re: Too good to be true - all 3 agar contamed [Re: Captainpaps]
    #20239862 - 07/07/14 10:54 PM (10 years, 6 months ago)

I feel like pics would help a little.  Is there any clean rhizomorphic mycelium at all or??


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.

Extras: Filter Print Post Top
Invisibled0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
Re: Too good to be true - all 3 agar contamed [Re: BLINKfan420]
    #20239881 - 07/07/14 10:58 PM (10 years, 6 months ago)

There definitely is on all three. The one has some very fluffy black mold I'm not sure if I should open that one. I could add pics tomorrow.


--------------------

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
Trusted Cultivator
Re: Too good to be true - all 3 agar contamed [Re: d0urd3n]
    #20240865 - 07/08/14 06:00 AM (10 years, 6 months ago)

Quote:

I think that is what gave them their color which is why I didn't see them.



If you smell the scalpel just after you make the transfer it can give you an idea of what you just transferred.


--------------------

Extras: Filter Print Post Top
Invisibled0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
Re: Too good to be true - all 3 agar contamed [Re: Kizzle]
    #20243713 - 07/08/14 06:10 PM (10 years, 6 months ago)

Well I made the transfers. I didn't have a scalpel so I used a shitty pocket knife. I couldn't get it red hot. I did wipe iso on it and then flame it though. It left black spots where I transferred. I couldn't see the myc on the knife I wonder if I should have taken large pieces. I know about the size of a grain of rice or smaller is best but I just couldn't see it. That was probably a mistake. I kept the jars just in case.

My sterile technique was a lot better I felt like this time. It got very hot wearing the mask though.

Do you guys think I will be okay? I didn't want to take large pieces because of the contams that were already in there.

I think I took way to small of pieces considering I couldn't even see them. Guess I will find out but it would be nice to have some sort of idea. Thanks.


--------------------

Edited by d0urd3n (07/08/14 06:16 PM)

Extras: Filter Print Post Top
OfflineMdahmer
Aloysius devadander abercrombie
Male User Gallery


Registered: 04/05/14
Posts: 2,516
Last seen: 7 years, 5 months
Re: Too good to be true - all 3 agar contamed [Re: d0urd3n]
    #20243920 - 07/08/14 06:37 PM (10 years, 6 months ago)

You really need a scalpel man, or an xacto knife which is what I use mostly...

I made is thing before I had a torch to get my scalpel super hot..heating with an alcohol lamp for too long made the handle too hot, with a torch it's just a hot second and the whole blades red.

You just need something thin and kinda sharp, the knife blade is probably too thick and only the very tip is most likely getting sterilized and that makes things dangerous.

Extras: Filter Print Post Top
Invisibled0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
Re: Too good to be true - all 3 agar contamed [Re: Mdahmer]
    #20244223 - 07/08/14 07:33 PM (10 years, 6 months ago)

Yeah I couldn't find a scalpel and I already had everything set up. I thought the blade was thin enough to get red. Anyways I was mostly concerned with getting enough myc on there. Do you think I got enough or should I just start over instead of wasting time waiting for nothing to grow when I could do another transfer from the first petris or start from spores again.


--------------------

Extras: Filter Print Post Top
Invisibled0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
Re: Too good to be true - all 3 agar contamed [Re: d0urd3n]
    #20276606 - 07/15/14 12:56 PM (10 years, 6 months ago)

Well there is only myc on one. But I don't see contams on any of the plates. Guess I will start from spores again for the others. RR said to boil the contamed jars for 30 mins but I am wondering if I can boil them when I used plastic lids? They are the ones from walmart has anyone done this before? Did a search and came up with nothing.

Thanks.


--------------------

Extras: Filter Print Post Top
OfflineCaptainpaps
Babaji NA MA AUM
Male User Gallery


Registered: 11/24/13
Posts: 1,533
Last seen: 8 years, 5 months
Re: Too good to be true - all 3 agar contamed [Re: d0urd3n]
    #20279233 - 07/15/14 11:08 PM (10 years, 6 months ago)

I pressure cook with the ball brand plastics you can find at walmart, they work fine


--------------------




Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Too good to be true - all 3 agar contamed [Re: d0urd3n]
    #20279304 - 07/15/14 11:45 PM (10 years, 6 months ago)

Quote:

d0urd3n said:
Well there is only myc on one. But I don't see contams on any of the plates. Guess I will start from spores again for the others. RR said to boil the contamed jars for 30 mins but I am wondering if I can boil them when I used plastic lids? They are the ones from walmart has anyone done this before? Did a search and came up with nothing.

Thanks.




yes thats fine


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Bags   MagicBag.co All-In-One Bags That Don't Suck   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Re: mold in agar plate Anonymous 13,428 3 12/04/99 12:35 AM
by Anonymous
* Re: Why do I want to grow mycelium on Agar?? Anonymous 2,595 3 02/13/00 04:07 PM
by AnubisRonin
* golden teacher agar asking fugu 3,168 6 01/31/03 12:59 PM
by debianlinux
* agar/peroxide conc. iudexk 1,369 1 08/27/01 01:17 PM
by jonnyshaggs420
* Prisoner #1's Agar Substitute
( 1 2 all )
Unknown 6,829 26 03/25/03 05:40 AM
by Prisoner#1
* Why grow on agar? stcore 2,057 1 07/29/02 05:03 PM
by bluhoney
* Wedge of light growth on agar
( 1 2 all )
Parafaragaramus 1,344 27 03/05/18 09:28 PM
by Mr.Kaption
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 21,904 38 05/11/01 11:08 AM
by MAIA

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,063 topic views. 34 members, 116 guests and 32 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.018 seconds spending 0.004 seconds on 12 queries.