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Offlinekjb1891
MycoManiac

Registered: 07/17/02
Posts: 341
Last seen: 5 years, 3 months
Can you use too much spore solution?
    #2023089 - 10/19/03 06:19 PM (13 years, 1 month ago)

I know that when inoculating BRF or grains in jars, the more spore solution you use, the faster colonization will be. But can you use too much? How much would be too much for 1/2 pints, pints, and quarts?


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Anonymous

Re: Can you use too much spore solution? [Re: kjb1891]
    #2023094 - 10/19/03 06:20 PM (13 years, 1 month ago)

if you use too much it will change the water content of the substrate. i think one syringe for 6 jars would speed it up quite a bit without adding too much water...correct me if im wrong.

~J-Slice~


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Invisiblemicro
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Registered: 05/09/03
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Re: Can you use too much spore solution? [Re: ]
    #2023101 - 10/19/03 06:23 PM (13 years, 1 month ago)

Yeah -- you'd need a lot of spore solution to upset a jar -- probably more than half a whole syringe per jar.


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(Avatar is Maxxy, a character by Mizzyam, RIP)


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Offlinekjb1891
MycoManiac

Registered: 07/17/02
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Re: Can you use too much spore solution? [Re: kjb1891]
    #2023434 - 10/19/03 08:27 PM (13 years, 1 month ago)

So, how much of a difference in colonization speed do you think using 3cc's instead of 1-1.5cc's for a pint jar make?


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OfflineGrapefruity
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Registered: 08/07/03
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Re: Can you use too much spore solution? [Re: kjb1891]
    #2023526 - 10/19/03 09:11 PM (13 years, 1 month ago)

i used 3 cc on one jar...it colonized fully in 2 weeks...the others with 1cc-1.5cc colonized in 3 weeks...i guess it is worth it if ya have alot of spore solution


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OfflineShrooomKing
Revolving

Registered: 09/12/03
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Re: Can you use too much spore solution? [Re: Grapefruity]
    #2023682 - 10/19/03 10:02 PM (13 years, 1 month ago)

Well my cat has a crazy notion that if she alters the amount of water by making the substrate a little dry then using about half a syringe on a 1/2 pint cake, her cake should grow at an accelerated speed. By then again she's a little off.


--------------------
A driver knows that it is not the road that is his biggest obstacle, rather it is the poles.
A great driver understands, the poles will always be there, and he must learn to adjust.

I can't keep doing this on my own... with all these... people. -daniel plainview


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Offlinelittlejohn747
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Registered: 09/18/03
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Re: Can you use too much spore solution? [Re: ShrooomKing]
    #2024015 - 10/20/03 12:07 AM (13 years, 1 month ago)

well what your cat is saying makes sense....i would was thinking about making a ton of solution using the karo tek and than switching the solution for water.....tell your cat to start posting some results when he/she gives it a shot


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OfflineDMJ
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Registered: 10/10/03
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Re: Can you use too much spore solution? [Re: littlejohn747]
    #2024771 - 10/20/03 08:10 AM (13 years, 1 month ago)

Well, a friend of mine says that it may seem faster, as there are more innoculation points. He says he reduced the 'norm' of 1 CC per injection site(which totals 4CC per jar), to 2-3 DROPS (not CC's) just drops per injection site, and he says he's had terriffic luck. 100% colonization in 14 days. It is his opinion that myc growth has to do more with parameters, than how much solution you use. He has now innoculated over 100 jars with the same syringe!! And still has 1/4 of it left! You try!


--------------------
I come alive in the darkness.


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Offlinelysergic
Mycophile!
Registered: 06/09/03
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Re: Can you use too much spore solution? [Re: DMJ]
    #2024855 - 10/20/03 09:40 AM (13 years, 1 month ago)

The more spores you have in the jar, the more spores their are to turn into mycelia. I'd recommend just making your spore syringes REALLY dark, rather than using more liquid.


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PsiloKitten said:
Just give em a little more time, the iraqis are making great progress. And this is unorganized. Wait till they get organized.


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Invisiblepsilomonkey
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Registered: 08/08/03
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Re: Can you use too much spore solution? [Re: lysergic]
    #2024870 - 10/20/03 10:03 AM (13 years, 1 month ago)

I was wondering about this, with more spores you are going to get more dikaryons, which will result in more, smaller networks? Can multiple dikaryons of the same strain interconnect to form a fruit body?
If not I would expect more but smaller fruits from dark syringes, and possible more aborts?

EDIT: When I say strain, I don't mean a 'pure strain' like you get from cloning a fruit, but say from spores from the same cap.


Edited by psilomonkey (10/20/03 10:09 AM)


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Invisiblemicro
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Re: Can you use too much spore solution? [Re: psilomonkey]
    #2024941 - 10/20/03 10:57 AM (13 years, 1 month ago)

You're going to get dikaryons in almost every case, anyway -- unless you are only injecting like 1-5 spores, which would probably take a shitload of time to colonize. You're adding at least tens of thousands of spores (that number was pulled out of my ass, but you get the idea) so you'll almost always have "mating." The dikaryotic hyphae will outrun the few monokaryotic ones that might be there that are incompatable.

--
Micro


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(Avatar is Maxxy, a character by Mizzyam, RIP)


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OfflineDMJ
creepyatmosphere

Registered: 10/10/03
Posts: 233
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Re: Can you use too much spore solution? [Re: micro]
    #2024972 - 10/20/03 11:14 AM (13 years, 1 month ago)

Yeah, what Micro said!

You only need 2 spores to innoculate (like herb, supposing one is weaker), so I read in the Shroomery. So my cousins cousin figured that if spores are microscopic, than there should be that ass# in at least ONE drop of solution. He's not as learned as the guy above, but he said the amount of spores in a single drop (the ass#) should be more than plenty. Although he was told to use one CC per site, his syringe was in ML, so he didn't know how to convert it. Seriously, if you have what he likes to call a 'pregnant syringe' , try just a couple of drops, and could you let me know how it goes?!? Peace!


--------------------
I come alive in the darkness.


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