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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 3 hours, 5 minutes
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Re: Where are my contams coming from? [Re: i GrOw StUFF]
#20232932 - 07/06/14 04:35 PM (10 years, 6 months ago) |
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i would also agee to start at the lids and switch out the poly and bent/rusted/pitted lids, those are your first line of defense lol, a flow hood would kick ass but you would still need to perfect your sab work, can't clean cultures with a hood
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 10 years, 4 months
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Re: Where are my contams coming from? [Re: cronicr]
#20232976 - 07/06/14 04:43 PM (10 years, 6 months ago) |
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Quote:
cronicr said: i would also agee to start at the lids and switch out the poly and bent/rusted/pitted lids, those are your first line of defense lol, a flow hood would kick ass but you would still need to perfect your sab work, can't clean cultures with a hood
Little confused on that last part... but I think you must learn to deal with a SAB before moving to flowhood also. You just learn so much about technique and planning. It's like college before graduate school.
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 3 hours, 5 minutes
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Re: Where are my contams coming from? [Re: i GrOw StUFF]
#20232987 - 07/06/14 04:45 PM (10 years, 6 months ago) |
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Quote:
i GrOw StUFF said:
Little confused on that last part... b
your not supposed to put contaminated cultures in front of a hood, it just blows the spores around so even if you have a flowhood you still need a sab
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 10 years, 4 months
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Re: Where are my contams coming from? [Re: cronicr]
#20233025 - 07/06/14 04:54 PM (10 years, 6 months ago) |
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ohhh I understand what you said now, yeah, don't want to put an absolute sporulating mess in front of a hood.
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 10 years, 4 months
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Re: Where are my contams coming from? [Re: i GrOw StUFF]
#20233049 - 07/06/14 05:01 PM (10 years, 6 months ago) |
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http://www.fungi.com/search-results.html?keywords=filter+discs&page=2
If you aren't interested in buying a 100 pack or what not, you can buy a tiny amount and then use a quarter to trace out circles on them. Cut out the circles with a pair of scissors.... A ten pack of wide mouth sized discs can make 80 filters cut up. Just silicone them on there.
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Where are my contams coming from? [Re: i GrOw StUFF]
#20233077 - 07/06/14 05:06 PM (10 years, 6 months ago) |
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I will likely get sfd's soon. But I've never had poly fail for me and its not just me, many many cultivators use it.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 3 hours, 5 minutes
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Re: Where are my contams coming from? [Re: Ghatti]
#20233082 - 07/06/14 05:07 PM (10 years, 6 months ago) |
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i myself use poly alot just because i always got bags of it kicking
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 7 days
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Re: Where are my contams coming from? [Re: cronicr]
#20233219 - 07/06/14 05:36 PM (10 years, 6 months ago) |
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Quote:
If you are trying to say SAB = Flowhood that is just silly. It is the difference between 99% and not. This is proven.
Proven how? Why is it silly?
I can tell you if you leave something open long enough in front of a flowhood it will become contaminated. That's necessarily so in a SAB. Although lose the advantage of blowing away any contaminants you stir up while you're working.
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 10 years, 4 months
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Re: Where are my contams coming from? [Re: Kizzle]
#20233331 - 07/06/14 06:06 PM (10 years, 6 months ago) |
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I'm just trying to help the OP on his projects. I don't want to get into a still air box vs. flowhood conversation.
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Where are my contams coming from? [Re: i GrOw StUFF]
#20233561 - 07/06/14 06:42 PM (10 years, 6 months ago) |
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I am however surprised that no one has had an issue like mine where a lid has some form of air gap or somehow the poly has failed.
I wonder if poly can somehow harbor spores in it. I roll it up very tight and pull the tip through with pliers until I can really pull no more, then snip off the excess. It's pretty tight in there.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 7 days
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Re: Where are my contams coming from? [Re: Ghatti]
#20233634 - 07/06/14 06:54 PM (10 years, 6 months ago) |
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If the filter gets wet contaminants can pass through it.
Did you happen notice if the mold started right around your agar wedge or first appeared somewhere else on the surface?
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Where are my contams coming from? [Re: Kizzle]
#20233678 - 07/06/14 07:04 PM (10 years, 6 months ago) |
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I didn't pay attention to where the filter was relative to where it began on the control jars that have never been opened.
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Where are my contams coming from? [Re: Ghatti]
#20233965 - 07/06/14 07:51 PM (10 years, 6 months ago) |
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So I just inoculated a few jars whose lid I had flipped back the right way (gasket down) and interestingly enough a few of the tops were extremely easy to get off.
I'm not sure if this means they got out of round a bit and even screwed down, didn't make a very good seal all the way around or if it means my pc didn't get hot enough to soften the gasket enough where it could get a super grip.
In the past, in my experience, jars sealed the correct way take a good deal of force to "pop".
For your viewing pleasure here is the pc I use minus the pressure gauge, my models only have the rocker and I own 2 of them
Edited by Ghatti (07/06/14 08:02 PM)
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 3 hours, 5 minutes
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Re: Where are my contams coming from? [Re: Ghatti]
#20234120 - 07/06/14 08:22 PM (10 years, 6 months ago) |
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Quote:
Ghatti said: I am however surprised that no one has had an issue like mine where a lid has some form of air gap or somehow the poly has failed.
I wonder if poly can somehow harbor spores in it. I roll it up very tight and pull the tip through with pliers until I can really pull no more, then snip off the excess. It's pretty tight in there.
i haven't had issues with it except last year i put a red hot syringe through which left an ugly gaping hole in the poly lol
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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StygianKnight
A Mushroom

Registered: 03/12/12
Posts: 2,717
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Re: Where are my contams coming from? [Re: Ghatti]
#20234331 - 07/06/14 08:57 PM (10 years, 6 months ago) |
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When I'm not fighting something annoying like penicilium I can get 100% out of a SAB, the key is to be patient and let things fall before you do your work. I used to get a lower percentage then upped the time I waited between setup up the box and doing the transfer which helped a lot.
Quote:
Ghatti said: The only visible contam is on top of the grains so I'm leaning towards my lids.
Rockin or not isn't the problem, I run my PC at 14psi for 120m cause the rocking annoys me, and it works just fine. I do agree, if the contam is always starting on top, it's not the PCing.
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Where are my contams coming from? [Re: StygianKnight]
#20234342 - 07/06/14 08:58 PM (10 years, 6 months ago) |
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The lack of a super seal make me think the lids are somewhat warped.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Where are my contams coming from? [Re: Ghatti]
#20234356 - 07/06/14 09:00 PM (10 years, 6 months ago) |
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Quote:
Ghatti said: The lack of a super seal make me think the lids are somewhat warped.
Lids are cheep get some new ones.
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Where are my contams coming from? [Re: MudaFuka]
#20234477 - 07/06/14 09:20 PM (10 years, 6 months ago) |
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Definitely on the agenda tomorrow. The end result should be a good learning experience for me and the community. You can do everything by the book but something as small as a knick or ever so slightly warped lid can stop you dead in your tracks.
I've actually never heard of lids "going bad" from being dropped or banged on etc.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Where are my contams coming from? [Re: Ghatti]
#20234496 - 07/06/14 09:25 PM (10 years, 6 months ago) |
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Yah. Lids aren't something I really gave much thought before.
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Mushiez



Registered: 04/28/14
Posts: 1,057
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Re: Where are my contams coming from? [Re: MudaFuka]
#20234694 - 07/06/14 09:56 PM (10 years, 6 months ago) |
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^what everyone said^
1. always wash cause why not, it takes a full 15 seconds and a toilet brush. it's good practice and don't want our grains to stick n clump to the sides 2. rubber side down or up is only pertinent to PF tek & is outdated anyway. Grain jars: always rubber down, you need a good seal. the idea is to isolate your GE exclusively to your SFD/poly/tyvek filter 3. rubber seal must be functional always. if your lids rubber is gone/burnt off/corroded, get some new in.
I've got me a collection of 20 or so toasted lids 
All my cacti's fault 
Lol @ cactus mescaline extraction, that xylene shit eats through lid rubber so fast
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