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Invisibleazur
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Re: Where are my contams coming from? [Re: Ghatti]
    #20230270 - 07/05/14 10:42 PM (10 years, 6 months ago)

Pc your scalpel and toss the tyvek sleeves


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Offlinei GrOw StUFF
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Re: Where are my contams coming from? [Re: Ghatti]
    #20230277 - 07/05/14 10:43 PM (10 years, 6 months ago)

Well it sounds like you are really dedicated to this hobby. :thumbup:........  If you rule out the dishes and polyfil, it has to be the transfer.  Remember, even if you are really good with your transfer technique, a glove box is still going to have an unwanted failure rate.  With a glovebox and transfers, you win some and you lose some.  Build a cheap flow hood man.  Few hundred bucks is all.


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The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.

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InvisibleMudaFuka
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Re: Where are my contams coming from? [Re: i GrOw StUFF]
    #20230297 - 07/05/14 10:48 PM (10 years, 6 months ago)

Most of us don't use a hood and we don't have contam problems.


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AMU
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Offlinei GrOw StUFF
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Re: Where are my contams coming from? [Re: MudaFuka]
    #20230347 - 07/05/14 10:58 PM (10 years, 6 months ago)

Quote:

MudaFuka said:
Most of us don't use a hood and we don't have contam problems.




Most that do transfers in a glove box have a nice sized failure rate.  Once you graduate to synthetic filter discs and flowhoods you will look back and wonder why you didn't do it sooner.  It's the difference between a 99% success rate and not.

:thumbup:


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The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.

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Re: Where are my contams coming from? [Re: i GrOw StUFF]
    #20231204 - 07/06/14 06:53 AM (10 years, 6 months ago)

really because most people including myself get 99 for the most part with nuthing more then sabs:shrug: most people that are using gloveboxes should be using sabs..so no it's not the difference between 99 and not it's having the technique down or not


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InvisibleGhatti
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Re: Where are my contams coming from? [Re: cronicr]
    #20231399 - 07/06/14 08:37 AM (10 years, 6 months ago)

I would use the scalpel on myself if I had to pc it for every time I used my box.

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Invisibleazur
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Re: Where are my contams coming from? [Re: Ghatti]
    #20231405 - 07/06/14 08:41 AM (10 years, 6 months ago)

I didn't say pc scalpel every time. I said pc it. You are having serious contam issues. Do a serious clean up


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
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InvisibleGhatti
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Re: Where are my contams coming from? [Re: azur]
    #20231528 - 07/06/14 09:46 AM (10 years, 6 months ago)

So this escalated faster than I could have wished for. I had a case of jars I had yet to get to from a week or so ago and this be them



Soaked wbs, dryed and pc'd for at least 90 mins at 15 psi. These jars have never been opened so its either the pc or my lids. Agreed?

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Invisibleazur
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Re: Where are my contams coming from? [Re: Ghatti]
    #20231606 - 07/06/14 10:18 AM (10 years, 6 months ago)

Agreed. You really need to do a thorough cleaning of your house to, since you've been breeding contams


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
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InvisibleGhatti
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Re: Where are my contams coming from? [Re: azur]
    #20231660 - 07/06/14 10:35 AM (10 years, 6 months ago)

A thorough cleaning is always in order but I never open any junk inside to limit my spore load.

I'm really puzzled what it could be though. My 15psi weight rocks well for 90mins and my lids are made no different than any others I don't believe. My poly is so tight I probably couldn't get a needle through it.

Hopefully simply flipping my lids will suffice. Although I won't know for a week or two.
Wonder if there is a way to test quicker.

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Invisibleazur
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Re: Where are my contams coming from? [Re: Ghatti]
    #20231680 - 07/06/14 10:44 AM (10 years, 6 months ago)

Clean man. If something is getting in your lids thrn something csn come out of your lids.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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InvisibleGhatti
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Re: Where are my contams coming from? [Re: azur]
    #20231690 - 07/06/14 10:47 AM (10 years, 6 months ago)

Lol again, agreed, but its redundant until I figure out where its all going wrong.

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Invisibleblindingleaf
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Re: Where are my contams coming from? [Re: Ghatti]
    #20231866 - 07/06/14 11:56 AM (10 years, 6 months ago)

is the rocker going crazy or is it rocking gently, like 1-2 times every 2-3 minutes? 
I put a wooden clothespin on mine for added weight, so the PSI goes up to about 17 during the cycle.  sometimes, i can get it to where there is literally only 3-4 weight "rattles" during the entire PC cycle, and the gauge reads about 15.5-16 psi, thats with the small amount of additional weight tho.


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MICROBIAL HUSBANDRY!!!!

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InvisibleGhatti
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Re: Where are my contams coming from? [Re: blindingleaf]
    #20231893 - 07/06/14 12:07 PM (10 years, 6 months ago)

My stove is finicky so some cycles it rocks constantly and some I can get it going just 3-4 a minute. Even marking it, its hard to hit the sweet spot.

The only visible contam is on top of the grains so I'm leaning towards my lids.

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Re: Where are my contams coming from? [Re: Ghatti]
    #20231943 - 07/06/14 12:27 PM (10 years, 6 months ago)

definitely try adding a little weight to it then, that will help with the constant rocking.  3-4 times a minute is a lot, its constantly going thru psi rises and drops.  u want the pressure to stay steady.  i think ur right tho about the lid, cause it is starting on the top, and even if its rocking a lot, 1.5-2 hours of cooking should still do the trick
can u blow thru the filter when u hold it up to ur mouth?  u don't want it stuffed so tight that there is no GE


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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InvisibleMudaFuka
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Re: Where are my contams coming from? [Re: blindingleaf]
    #20231959 - 07/06/14 12:31 PM (10 years, 6 months ago)

My stove top is un level so my weight rocks more than I would like but my gauge stays at 15 PSI and I have left un inoculated jars for weeks without issue. It's got to be the lids or filters. I hope flipping the lids works fore you.


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AMU
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Invisibleblindingleaf
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Re: Where are my contams coming from? [Re: MudaFuka]
    #20232001 - 07/06/14 12:42 PM (10 years, 6 months ago)

are your grains moist enough that when u squeeze them hard or bite into them, they easily break?  they look like they have not expanded enough and are a little on the dry side
i don't know if this is true or not, but I'm guessing that a really dry grain will still be totally dry on the inside, and because water conducts heat, PCing/sterilizing grains is more effective when they are moist thru and thru.  if the grain is still totally dry in the middle, it will take a lot longer for it to reach 250f.  so maybe try hydrating them more, so the PC cycle is more effective.  thats a guess tho


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Invisibleazur
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Re: Where are my contams coming from? [Re: blindingleaf]
    #20232032 - 07/06/14 12:50 PM (10 years, 6 months ago)

I'm still blaming technique of transfer, dirty air, lids, and tyvek sleeves. Mostlylids though.


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A cube is NOT a cube.

FALL IN LOVE WITH LC
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InvisibleGhatti
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Re: Where are my contams coming from? [Re: blindingleaf]
    #20232047 - 07/06/14 12:53 PM (10 years, 6 months ago)

They are fully hydrated. I will examine the true top of the lid (metal) for pits and burrs which might create air gaps and also my poly when I clean them later.

Still though if anything my poly is too tight which wouldn't do anything in terms of mold but more likely bacteria.

To be on the safe side the next batch I pc I will replace the poly though.


How can you blame technique when these jars haven't been opened? You blame my pc technique? Lol

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Offlinei GrOw StUFF
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Re: Where are my contams coming from? [Re: cronicr]
    #20232916 - 07/06/14 04:32 PM (10 years, 6 months ago)

Quote:

cronicr said:
really because most people including myself get 99 for the most part with nuthing more then sabs:shrug: most people that are using gloveboxes should be using sabs..so no it's not the difference between 99 and not it's having the technique down or not





Thats awesome.  You got some skills buddy :thumbup: :smile:..... My only point was flowhoods are better than SAB and gloveboxes in the long run.  I don't always suggest getting a flowhood to someone, but the OP seems like he is really into the hobby and might just need a little push to make the next step :shrug:.  If you are trying to say SAB = Flowhood that is just silly.  It is the difference between 99% and not.  This is proven.

To the OP, if you haven't opened those lids and they contaminated, with mold of all things, it's your filter.  Get rid of the poly man.  Just my opinion. Just my two cents given the info you have provided.  Don't hate me for it.


--------------------
The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.

Edited by i GrOw StUFF (07/06/14 04:33 PM)

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Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Kraken Kratom Red Vein Kratom


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