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InvisibleGhatti
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Re: Where are my contams coming from? [Re: Ghatti]
    #20590237 - 09/19/14 08:23 PM (10 years, 3 months ago)

So my control plates are still clean although 3 days isn't quite long enough to judge even with an overly nutritious agar mix. I dropped wedges on the very edge of the jars pretty much up against the glass.

The hope is if something got in during inoculation it will germinate and I will clearly see it apart from the agar.

I didn't shake or loosen the grain in these jars in any way so I would imagine an outbreak to start on top of the grains or very close.

I think in this way if I don't see any competitor growth in a week or so on my plates or grain I can affirm one of two things.
1: My contams were getting in during inoculation and the small change to a positive pressure box fixed it or.....

2: I eventually spawn the clean looking jars and they turn green and I can blame my culture carrying something along.

Is this agreeable?

Or is there another form of control I should put in place?

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Invisibleelasticaltiger
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Re: Where are my contams coming from? [Re: Ghatti]
    #20590305 - 09/19/14 08:43 PM (10 years, 3 months ago)

:nodofunderstanding:


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EZEKIEL 23:20

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InvisibleGhatti
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Re: Where are my contams coming from? [Re: elasticaltiger]
    #20590383 - 09/19/14 09:03 PM (10 years, 3 months ago)

I will leave 1 jar unopened from the pc cycle and "fake" put a wedge in one with the regular sab and repeat with the box pressurized.

I think this will cover everything. Then I can quit bugging yall with this and in doing this maybe I can find my own answer or someone can spot it more easily.

Edited by Ghatti (09/19/14 09:04 PM)

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InvisibleGhatti
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Re: Where are my contams coming from? [Re: Ghatti]
    #20608166 - 09/23/14 07:33 PM (10 years, 3 months ago)

Little update. I had dropped some wedges on the far side of jars pretty much against the glass and low and behold if I don't have some sort of white colony starting on the opposite side of the jar as well.

So that pretty much says that whatever it is is getting in during inoculation and whatever this is looks EXTREMELY like mushroom mycelium.

I have some on a plate that I will show in a day or two once it grows out some. These plates were opened and sat in all 4 corners of my sab as control markers, all 4 have colonies on them.

This contam I wouldn't be able to tell apart from mushroom spores germinating, which I also have going from a print so I'll do a side by side as well. Any requests to see it under a scope as well? Figured out how to take good quality pics through my scope.

I acquired some time to work this evening with a hood so if the jars I did tonight fail I guess that means my original culture is hiding something as well right?

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Invisibleazur
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Re: Where are my contams coming from? [Re: Ghatti]
    #20608483 - 09/23/14 08:53 PM (10 years, 3 months ago)

I thought you brought on a new culture?


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InvisibleGhatti
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Re: Where are my contams coming from? [Re: azur]
    #20608507 - 09/23/14 08:57 PM (10 years, 3 months ago)

I have a print on agar that's a day or two out from the first of a few transfers. I HAVE restarted cultures 3 times now with the same problem. So I figured use the old culture with the use of the hood while I had it and when the new culture is transferred a few times I'll give it a go as well.

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OfflineKizzle
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Re: Where are my contams coming from? [Re: Ghatti]
    #20608509 - 09/23/14 08:58 PM (10 years, 3 months ago)

Pics would be great. Just remember if wanting to identify it you'll need to view a mold colony that has already started sporulating .


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Invisibleazur
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Re: Where are my contams coming from? [Re: Ghatti]
    #20608517 - 09/23/14 08:59 PM (10 years, 3 months ago)

Shweet


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A cube is NOT a cube.

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InvisibleGhatti
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Re: Where are my contams coming from? [Re: azur]
    #20608530 - 09/23/14 09:01 PM (10 years, 3 months ago)

Pics won't really help atm since the colony is so small. It really just looks like mushroom spores germinating in a perfectly radial fashion.

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InvisibleGhatti
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Re: Where are my contams coming from? [Re: Ghatti]
    #20632323 - 09/28/14 07:13 PM (10 years, 3 months ago)

So I promised a pic of the cooties that have been plaguing me. Can anyone ID it? This was a print scraped onto a plate and neglected. The outside colony popped up and its not alone. I see this on almost all of my plates that get a contam.

Got it on all 4 of my control plates last time I did anything in my sab. Been waiting for it to go wild and sporelate visibly but it just kinda hangs out.


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