Home | Community | Message Board

The Spore Depot
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: < First | < Back | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | Next >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 7 days
Trusted Cultivator
Re: Where are my contams coming from? [Re: Ghatti]
    #20585320 - 09/18/14 07:30 PM (10 years, 3 months ago)

Maybe you should give it one last attempt and try a grow bag. They can be completely colonized and even produce the first flush before being exposed to anything outside the sterile environment of the bag. It'd eliminate any possibility of contamination being introduced during the spawn run because they don't need to be spawned.


--------------------

Extras: Filter Print Post Top
InvisibleMudaFuka
Poppin bottles
Male User Gallery


Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
OG Cultivator
Re: Where are my contams coming from? [Re: Kizzle] * 1
    #20585374 - 09/18/14 07:43 PM (10 years, 3 months ago)

I've been inoculating jars of sterilized grain/substrate mixes with liquid inoculant in my SAB and fruiting them out of the jars. They stay in sterile conditions until its time to fruit.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: Where are my contams coming from? [Re: MudaFuka]
    #20585407 - 09/18/14 07:52 PM (10 years, 3 months ago)

A grow bag would be equivalent to a grain jar with a SHIP I would think. Problem is I don't have a clean syringe and as far as your suggestion Muda, I wouldn't trust an LC if I can't successfully inoculate a grain jar with an agar wedge with any consistency.

I'm going to let the jars go that I knocked up once I made the hepa filtered positive pressure box. It probably won't work but it only cost me 8 bucks. Thought was to hopefully knock back some of the funk I was bringing in when I put my arms in. Looking back at it it has a very very low chance of changing anything.

Extras: Filter Print Post Top
InvisibleMudaFuka
Poppin bottles
Male User Gallery


Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
OG Cultivator
Re: Where are my contams coming from? [Re: Ghatti] * 1
    #20585426 - 09/18/14 07:56 PM (10 years, 3 months ago)

I don't use LC I make non nutritive Liquid inoculant with Agar wedges and sterilised water. Second link in my sig.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: Where are my contams coming from? [Re: MudaFuka]
    #20585454 - 09/18/14 08:06 PM (10 years, 3 months ago)

Sadly I don't own a compatible blender. Even then I wouldn't trust opening a jar to pour it in or anything else really at this point.

I really should just get a syringe and do ms grows but none of my jars have ships so I'd still have to crack lids anyways so I'd likely be in the same boat as using wedges.

Extras: Filter Print Post Top
Invisibleazur
God of Fuck
Male User Gallery


Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: Where are my contams coming from? [Re: Ghatti]
    #20585474 - 09/18/14 08:09 PM (10 years, 3 months ago)

Pf tek


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


Extras: Filter Print Post Top
InvisibleMudaFuka
Poppin bottles
Male User Gallery


Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
OG Cultivator
Re: Where are my contams coming from? [Re: Ghatti]
    #20585492 - 09/18/14 08:13 PM (10 years, 3 months ago)

I open my jars for every inoculation, Even with syringes. I don't like the Idea of my needle coming into contact with a SHIP which is only sanitised and not sterile. It's a small risk I know but it still doesn't sit right with me.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: Where are my contams coming from? [Re: MudaFuka]
    #20585504 - 09/18/14 08:14 PM (10 years, 3 months ago)

:whathesaid:


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum

Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: Where are my contams coming from? [Re: MudaFuka]
    #20585515 - 09/18/14 08:16 PM (10 years, 3 months ago)

I don't like the idea either, hence why none of my jars have them. But I've about resigned myself to the fact that contams are getting in during inoculation be it off of my gloves, my agar, or just floating around.

So cracking lids is likely a no-go unless I'm downstream on a hood.

Extras: Filter Print Post Top
Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 8,438
Trusted Cultivator
Re: Where are my contams coming from? [Re: Kizzle]
    #20585649 - 09/18/14 08:42 PM (10 years, 3 months ago)

Quote:

Kizzle said:
Maybe you should give it one last attempt and try a grow bag. They can be completely colonized and even produce the first flush before being exposed to anything outside the sterile environment of the bag. It'd eliminate any possibility of contamination being introduced during the spawn run because they don't need to be spawned.




... was gonna say. There are places that do the work for you.

Their rates are reasonable.

Fuck PM me and I'll prep some media for you to inoculate. You have to promise me you'll only use it for oysters though.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

Edited by elasticaltiger (09/18/14 08:42 PM)

Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: Where are my contams coming from? [Re: elasticaltiger]
    #20585682 - 09/18/14 08:48 PM (10 years, 3 months ago)

That's all I attempt to growz. I don't think its the media. I really think its happening when I inoculate so even if you sent me something sterile, it wouldn't be once I got a hold of it lol.

I don't think its the culture cause when I spawned the jars individually half were good. If it was the culture I woulda thought everything would go bad.

I don't think its the filters or media since I just switched to sfd's and I pc'd a group of jars and let them sit for 2 weeks with no contams.

So that really just leaves stuff getting in when I inoculate right?

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 7 days
Trusted Cultivator
Re: Where are my contams coming from? [Re: MudaFuka]
    #20585716 - 09/18/14 08:54 PM (10 years, 3 months ago)

Quote:

MudaFuka said:
I open my jars for every inoculation, Even with syringes. I don't like the Idea of my needle coming into contact with a SHIP which is only sanitised and not sterile. It's a small risk I know but it still doesn't sit right with me.



Put a piece of tape over the SHIP before sterilization When you want to inoculate the jar flame sterilize your needle. While still red-hot lightly touch the end of the needle to the center of the tape killing any contaminants in that spot on contact, hold it there for a few seconds as the needle cools so you don't damage your port and press it through.


--------------------

Extras: Filter Print Post Top
InvisibleMudaFuka
Poppin bottles
Male User Gallery


Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
OG Cultivator
Re: Where are my contams coming from? [Re: Kizzle]
    #20585748 - 09/18/14 09:02 PM (10 years, 3 months ago)

I don't use syringes very often anymore but that's a good tip.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 7 days
Trusted Cultivator
Re: Where are my contams coming from? [Re: MudaFuka]
    #20585794 - 09/18/14 09:11 PM (10 years, 3 months ago)

Yeah I don't either. I suppose because of the hatred I've acquired of contaminated LCs and spore syringes :shakefist: but not because it doesn't work.


--------------------

Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: Where are my contams coming from? [Re: Kizzle]
    #20585953 - 09/18/14 09:38 PM (10 years, 3 months ago)

So the control plates I opened for 10 secs, got bacteria in one of em. Guess I'll wait a few days and see if any mold germinates.

Hell bacteria I could deal with lol

If there's bacteria there's prolly spores on there too. I have the sterile air blowing down so I probably wafted my hand too close over the dish and the eddy's blow crap in.

I wonder since my air purifier uses a blower (centrifugal fan) could I not just put the 2 brand new hepas side by side and build a flange to fit it.

It wouldnt be laminar but it should be sterile and if I turned my tub on its side with the open end facing me and cut the bottom out and mounted the filters in I wonder if it wouldn't give me a nice little space to manipulate it seeing as it has walls and a roof?

Edited by Ghatti (09/18/14 09:50 PM)

Extras: Filter Print Post Top
OfflineMdahmer
Aloysius devadander abercrombie
Male User Gallery


Registered: 04/05/14
Posts: 2,516
Last seen: 7 years, 5 months
Re: Where are my contams coming from? [Re: Ghatti]
    #20586080 - 09/18/14 09:59 PM (10 years, 3 months ago)

i think you just need to maybe look at your SAB and make sure theres nothing wrong or odd about it and just work at your sterile tech. if you spawned all those jars by themselves and only half failed im guessing your just not very smooth with innoc jars and whatnot. i practiced with my old agar plates in my sab while watching TV doing transfers over and over then going to jars over and over until i felt like a machine. smoking a lot of herb helps.

i wouldnt quit growing after dragging this thread along, its just not right :noargument:

Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: Where are my contams coming from? [Re: Mdahmer]
    #20586113 - 09/18/14 10:05 PM (10 years, 3 months ago)

Lmao you have a point. Well I have a spare tub so I may try my idea anyways, what can it hurt at this point in the game anyways?

I'd love to work on my sterile technique but I honestly don't see how I can improve it. I feel like I've had so many failures that everything is just smothered in mold spores and even if I wash up I still get nasty by the time I go in my sab.

I do the same process as I do with my agar plates and they turn out fine 99% of the time.

Extras: Filter Print Post Top
InvisibleMudaFuka
Poppin bottles
Male User Gallery


Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
OG Cultivator
Re: Where are my contams coming from? [Re: Mdahmer]
    #20586115 - 09/18/14 10:05 PM (10 years, 3 months ago)

It took me a lot of practice to get good at sterile work. I fucked up well over 100 plates before I got 1 without bacteria.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

Extras: Filter Print Post Top
OfflineMdahmer
Aloysius devadander abercrombie
Male User Gallery


Registered: 04/05/14
Posts: 2,516
Last seen: 7 years, 5 months
Re: Where are my contams coming from? [Re: MudaFuka]
    #20586132 - 09/18/14 10:09 PM (10 years, 3 months ago)

yea me too with the bacteria, it was terrible. ive only seen mold twice that i remember except on old ass unwrapped plates but i would be for sure seeing bacteria, it was a matter of having clean enough growth away from it to get a good sized chunk for my jar lol.

i got a bunch of bacteria growing right now from a few different clones actually..fucking shit

Extras: Filter Print Post Top
InvisibleGhatti
Totally not a Federal Agent
 User Gallery
Registered: 06/23/14
Posts: 1,733
Re: Where are my contams coming from? [Re: MudaFuka]
    #20586140 - 09/18/14 10:10 PM (10 years, 3 months ago)

My plates are usually clean, my jars however look clean but grow green once spawned to cvg and introduced to fae.

Hey that sounded spiffy.

Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | Next >

Shop: OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Can Contam Spread From Jar To Jar?? (pf tek brf) PooGrower 4,880 7 12/04/02 02:18 AM
by Bi0TeK
* Casing, once contamed, substrate... TheShroomHermit 1,350 6 10/15/02 08:48 PM
by SixCee
* substrate transfer?!?! dets 3,017 8 11/13/02 12:29 AM
by SixTango
* Liquidplaya's 99% contam free grain -> X TransfTek liquidplaya 2,155 7 10/25/16 10:59 AM
by Greg
* Re: Contams in Honey Water Lana 934 2 07/16/00 07:30 AM
by Hippie3
* Where is this cobweb coming from? Golddoor 848 11 02/03/03 07:38 AM
by daussaulit
* transferring mycelium wikedanjel 4,251 13 01/10/03 03:54 AM
by debianlinux
* Noooooo I am a stupid person or something.. contam Splat 2,793 9 03/07/02 06:55 AM
by Seuss

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
16,992 topic views. 23 members, 113 guests and 14 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.009 seconds on 13 queries.