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Gorguss
Chaotic sums


Registered: 02/03/10
Posts: 710
Last seen: 1 month, 12 days
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Contamination Issues... *DELETED*
#20216222 - 07/02/14 06:55 PM (10 years, 6 months ago) |
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Post deleted by Gorguss
Reason for deletion: old outdated.
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Looking for Trichocereus Cacti
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March Hare
Stranger


Registered: 06/26/14
Posts: 315
Last seen: 10 years, 6 months
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Re: Contamination Issues... [Re: Gorguss]
#20216225 - 07/02/14 06:56 PM (10 years, 6 months ago) |
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Which contams?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Contamination Issues... [Re: Gorguss]
#20216229 - 07/02/14 06:59 PM (10 years, 6 months ago) |
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First a glove box is inferior to a SAB. The attached gloves make flame sterilization difficult and actually cause more air to move inside stirring up contams and pulling air in through the lid. Turn your glovebox into an SAB 
Second what kind of filters are you using?
Third, LC is sketch but if your syringes to grain are contaming that is not encouraging for your inoculate. What kind of contamination are you seeing? Is it always the same?
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CH HELL
Brain Sturgeon


Registered: 10/02/08
Posts: 6,610
Loc: mars
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Re: Contamination Issues... [Re: Gorguss]
#20216231 - 07/02/14 06:59 PM (10 years, 6 months ago) |
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Try preparing your coir differently. Bucket tek works fine most of the time but if you are having issues change coir brands and do a proper pasteurization.
Is it green? Trich? or other contam?
CH
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Contamination Issues... [Re: CH HELL]
#20216237 - 07/02/14 07:01 PM (10 years, 6 months ago) |
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I seriously doubt the bucket is his problem. Coir has been proven many times to be extremely contam resistant I have sterilized coir many times with excellent success.
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Gorguss
Chaotic sums


Registered: 02/03/10
Posts: 710
Last seen: 1 month, 12 days
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Re: Contamination Issues... [Re: Pastywhyte]
#20216239 - 07/02/14 07:02 PM (10 years, 6 months ago) |
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I know i cant flame sterilize but even when i wrap the syringes in foil and pressure cook 'em. and everything sterile.
I'm seeing trich. NEVER in the jars as they colonize, i;ve let some jars sit for so long they fruited inside the jar.
Using polyfil lids.
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Looking for Trichocereus Cacti
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Gorguss
Chaotic sums


Registered: 02/03/10
Posts: 710
Last seen: 1 month, 12 days
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Re: Contamination Issues... [Re: Gorguss]
#20216245 - 07/02/14 07:03 PM (10 years, 6 months ago) |
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I've also prepared straw from RR's vids, and Hpoo aged from the feild. Ive tried pasteurizing it in a jar with meat thermo like in RR's vids no diff outcome.
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Pastywhyte
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Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Contamination Issues... [Re: Gorguss]
#20216255 - 07/02/14 07:06 PM (10 years, 6 months ago) |
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Trich often is difficult to spot in a grain jar and also tricky to smell if you don't know what to smell for. Your making your own syringes? Personally I do not trust syringes to go directly to grains. I know it works for some people but I need agar to make grains work myself.
I am willing to bet its your syringes that are the problem. Do up some agar and see what that tells you. Get the gloves off your box and buy a blowtorch while your at it
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cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
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Re: Contamination Issues... [Re: Gorguss]
#20216259 - 07/02/14 07:09 PM (10 years, 6 months ago) |
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Quote:
Gorguss said:
I'm seeing trich. NEVER in the jars as they colonize, i;ve let some jars sit for so long they fruited inside the jar.
Using polyfil lids.
lots of molds including trich won't show there face on grains alot of the time so never count it out..pasty pretty much beat me too it
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March Hare
Stranger


Registered: 06/26/14
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Re: Contamination Issues... [Re: Gorguss]
#20216264 - 07/02/14 07:12 PM (10 years, 6 months ago) |
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Any chance you keep plants in the same room?
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CH HELL
Brain Sturgeon


Registered: 10/02/08
Posts: 6,610
Loc: mars
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Re: Contamination Issues... [Re: cronicr]
#20216266 - 07/02/14 07:13 PM (10 years, 6 months ago) |
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If it was the same culture from agar I would agree with you guys but he said it was several different cultures and multispore. Some coir contains Trich and is not killed off by bucket tek.
CH
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Gorguss
Chaotic sums


Registered: 02/03/10
Posts: 710
Last seen: 1 month, 12 days
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Re: Contamination Issues... [Re: Gorguss]
#20216281 - 07/02/14 07:19 PM (10 years, 6 months ago) |
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yes. These are MS syringes purchased from a reputable vendor.
I've also done G2G in this glove box with a spoon i wrapped in foul and cooked.
The only thing i can really see is that the coir is bad or something. I did oysters to rye grain in there too with success. 40 G2g and no contamination
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Gorguss
Chaotic sums


Registered: 02/03/10
Posts: 710
Last seen: 1 month, 12 days
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Re: Contamination Issues... [Re: Gorguss]
#20216286 - 07/02/14 07:20 PM (10 years, 6 months ago) |
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I've also never done agar. I have some pp5 containers and some potatoes as well as the agar agar but no yeast or dextrose.
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Looking for Trichocereus Cacti
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Contamination Issues... [Re: CH HELL]
#20216323 - 07/02/14 07:28 PM (10 years, 6 months ago) |
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Quote:
CH HELL said: If it was the same culture from agar I would agree with you guys but he said it was several different cultures and multispore. Some coir contains Trich and is not killed off by bucket tek.
CH
But the LC came from ms and it sounds like he is making his own syringes, but lets say that the coir is the issue. Thermal death for most trich species occures at 130 F sustained for 30 min which means I doubt the bucket is the problem but, assuming it is and assuming the trich was infused in the coir to begin with, OP needs to sterilize his coir first. I have used coir bricks where you could smell the trich in it. A run through the sterilzer and it works fine.
Quote:
Gorguss said: I've also never done agar. I have some pp5 containers and some potatoes as well as the agar agar but no yeast or dextrose.
You could use honey in place of Karo for the dextrose with no problems. Honey's anti fungal properties are greatly exaggerated.
Edited by Pastywhyte (07/02/14 07:33 PM)
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Contamination Issues... [Re: Pastywhyte]
#20216401 - 07/02/14 07:44 PM (10 years, 6 months ago) |
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Stop using a spoon for your G2G, just pour it instead.
Use a SAB instead of a GB. An SAB works better.
Make sure you wipe under the lip of your lids with paper towel with alcohol before you open the jar.
What are you using for filters?
I used to have lots of failures until I started using agar. I don't trust spores to be clean from anyone.
It's unlikely the fault of the bucket tek, however, you can remove the doubt by properly pasteurizing CVG. If you are still getting contamination before the first flush, then you know it's something else.
In my opinion, MS>grain>g2g in a glovebox with a spoon is a recipe for problems.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Quote:
SpitballJedi said: In my opinion, MS>grain>g2g in a glovebox with a spoon is a recipe for problems.
QFT
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Gorguss
Chaotic sums


Registered: 02/03/10
Posts: 710
Last seen: 1 month, 12 days
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Re: Contamination Issues... [Re: Pastywhyte]
#20216495 - 07/02/14 08:03 PM (10 years, 6 months ago) |
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Quote:
Pastywhyte said:
Quote:
SpitballJedi said: In my opinion, MS>grain>g2g in a glovebox with a spoon is a recipe for problems.
QFT
I suppose this is where im at then.
I just dont see how my arms and air movement inside will be cleaner then a sealed box thats bean sprayed thoroughly with lysol and oust. turning it into a SAB would mean removing the gloves that are shoulder length and simply not using oust and lysol and flaming the needle every time?
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Looking for Trichocereus Cacti
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Contamination Issues... [Re: Gorguss]
#20216533 - 07/02/14 08:12 PM (10 years, 6 months ago) |
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yep. Your gloves are likely causing air to get sucked in from somewhere. Those sprays aren't really killing anything. The gloves are also causing turbulence inside the box making contams float around. It's not the gloves per se as much as it's the fact that they are attached and creating a vacuum effect.
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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I know of 2 most reputable vendors that have had contamination problems lately. I'm betting you got dirty syringes. Contact your vendor and inquire if you have ruled all else out.
I had the same issue and had to go to agar and 3 transfers to get a clean culture
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