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OfflineMr. Penguin
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Registered: 03/26/14
Posts: 548
Last seen: 6 years, 9 months
Re: longest g2g (2g2g2g2g2g2g) [Re: cronicr]
    #20215305 - 07/02/14 02:29 PM (10 years, 6 months ago)

Can you elaborate a little more on the tested isolate? Do you mean spores on agar?

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Offlineblojo02184
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Registered: 05/15/13
Posts: 3,527
Loc: Maine
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Re: longest g2g (2g2g2g2g2g2g) [Re: Mr. Penguin]
    #20215353 - 07/02/14 02:43 PM (10 years, 6 months ago)

Yeah.
You drop a few hundred spores on a dish with either a swipe or a drop.
most of those germinate into monokaryotic mycelium.
Then each one finds a mate to become dikaryotic mycelium. This is what makes fruit bodies.

Now let's say 500 spores germinates at 80%.
You would have 400 monokaryotic genes.
They mate to make 200 dikaryotic genes. Without these no mushrooms.

Then you start trying to isolate just 1 of 200 genes to see if its any good.

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Offlinespacechildo
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Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Re: longest g2g (2g2g2g2g2g2g) [Re: cronicr]
    #20215558 - 07/02/14 03:42 PM (10 years, 6 months ago)

Quote:

cronicr said:
not worth it, i see this mistake often when people decide to clone store bought mushrooms. they think it's a quick way to get great genetics but in reality it's an old culture that will never produce yields worth a shit as it's been through five rounds of g2g and spawned at 1:10 ratios




what if ya cloned that store fruit, put it on grains and grew it out.
then take the prints they drop and start looking for a nice culture?
those spores would be genetically reset to a certain degree, and the new mycel young and vigorous.

or is that another :failboat: ?

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OfflinecronicrMFacebook
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Registered: 08/07/11
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Re: longest g2g (2g2g2g2g2g2g) [Re: spacechildo]
    #20215562 - 07/02/14 03:43 PM (10 years, 6 months ago)

nope thats perfectly fine to do so but so is simply printing the mushroom from the store:wink:


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: longest g2g (2g2g2g2g2g2g) [Re: cronicr]
    #20215965 - 07/02/14 05:46 PM (10 years, 6 months ago)

I never clone a fruit that was G2G more than once and usually not even that. Most of my MS grows intended for cloning I just nock jars with wedges.

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