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OfflineJaedos
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Registered: 06/14/14
Posts: 31
Last seen: 9 years, 6 months
WBS bulk spawn - Inconsistent jars...
    #20209205 - 07/01/14 02:34 AM (10 years, 6 months ago)

Hello all.  So I'm working my way through my first adventure in mushroom cultivation.  I used one of the wild bird seed teks to eventually spawn to bulk.  I followed the steps of PC'ing my quart jars after soaking and drying the bird seed.  I inoculated with flame sterilized needles.

I have three 1/4" - 3/8th holes in the lids.  Where I may have run into a problem is I think I used the wrong tape on the lids.  I had the cloth tape, which I thought was micropore.  It sounds like MP is the papertape.  Oi.

I proceeded with the innoculations anyhow.  I had read that the jars should be kept in darkness at between 80-85*F.  I did this for about a week and seemed to be having good solid growth on most jars. 

The Argentina was absolutely kicking ass.  I think where I may have messed up is that I covered the jars with a few thick towels to insulate them and keep it dark.  I'm wondering if this may have impacted the FAE though.

I read instructions that said after 5-7 days, shake the jars to distribute the growth. 

I did this and all seemed well.

I Innoc'd the jars on 6/17 (ignore the 6/19 on the labels, I had the date wrong).  I shook them about 6/24.  Today is 7/1 and I have noticed a few things.  The growth in the jaws that is flourishing does not seem as solid as it did at first.  Also I have some jars that don't seem to be doing anything at all, as if they're stalled.  I shook all of the jars, even the ones with minimal growth in them.

I'm also using four strains:
Argentina
B+
Ecuador
Golden Teacher

I found more instructions that read absolute darkness is not necessary.  So right now I have them sitting in the open on a shelf with shade from the ceiling light.  During the day they'll receive indirect day light, or I can hang more shade clothes on the shelves.

Here is where the jars are at currently.  Feedback is appreciated.  Oh, the jars were innoc'd with 3-4ml each.

Argentina:


B+:


Ecuador:


Golden Teacher:


Golden Teacher close up:


Any feedback is appreciated.

Edited by Jaedos (07/01/14 03:16 AM)

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OfflineJaedos
Stranger
Registered: 06/14/14
Posts: 31
Last seen: 9 years, 6 months
Re: WBS bulk spawn - Inconsistent jars... [Re: Jaedos]
    #20209206 - 07/01/14 02:36 AM (10 years, 6 months ago)

The bright white in the middle Argentina jar was a chunk of the pre-shake growth.  I don't seem to be getting that same thickness after the shake.

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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
Trusted Cultivator
Re: WBS bulk spawn - Inconsistent jars... [Re: Jaedos]
    #20209219 - 07/01/14 02:43 AM (10 years, 6 months ago)

Quote:

Jaedos said:
I had read that the jars should be kept in darkness at between 80-85*F.  I did this for about a week and seemed to be having good solid growth on most jars.



Jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.

Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.

Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.

P. Cubensis are a tropical species. You could colonize at 70F and fruit at 80F with great results.

Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.

You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.

You want direct/intense 6500K light on a 12/12 schedule for fruiting.

Optimal temps are mid 70s throughout the whole grow, but anywhere from 65F-80F is acceptable.

Incubation is outdated/uneeded unless temps in the range stated above cannot be kept.

The inside of the jar is always a few degrees warmer than the outside because the mycellium produces heat..mycellium tends to stall at temps above 86F , and contams thrive.

Fruiting at cooler temps tends to produce denser, meatier fruits, while fruiting at higher temps will often produce hollow, less dense stems.




Mycelium should be exposed to ambient room light from day of inoculation as has been known for many years.  Light is not a pinning trigger until after full colonization and an increase in air is given, and even then it's a secondary pinning trigger.
RR


Quote:

Jaedos said:
The Argentina was absolutely kicking ass.  I think where I may have messed up is that I covered the jars with a few thick towels to insulate them and keep it dark.  I'm wondering if this may have impacted the FAE though.



FAE(Fresh Air Exchange) is only important during fruiting, you mean GE(Gas Exchange). And it just might have impacted GE.

But again, there is no reason to incubate or keep them in darkness.

Quote:

Jaedos said:
I read instructions that said after 5-7 days, shake the jars to distribute the growth.



Shake once at about 30% colonization.....no time frame can be put on this, it happens when it happens.

Please remove the vendors name from your post, and take a moment to read thru the forum rules.

Rules Thread-READ BEFORE POSTING

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OfflineJaedos
Stranger
Registered: 06/14/14
Posts: 31
Last seen: 9 years, 6 months
Re: WBS bulk spawn - Inconsistent jars... [Re: PussyFart]
    #20209272 - 07/01/14 03:26 AM (10 years, 6 months ago)

Thank you sir.  I corrected the post and stripped the vendor names.

I guess this bites me for over researching, or not starting with a complete guide before hand.  I think a few of the posts I used were quite old, which might explain the advice to cover/box.

A little more reading shows as much as 4+ weeks for quart jars in some cases.  With that in mind, I'll just keep these shelved, indirectly lit, and watch them.

I'm also going to read all the cyan links in your post.  Thank you for the reply.

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OfflinecronicrMFacebook
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Registered: 08/07/11
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Last seen: 28 minutes, 30 seconds
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Re: WBS bulk spawn - Inconsistent jars... [Re: Jaedos]
    #20209312 - 07/01/14 03:50 AM (10 years, 6 months ago)

isn't it funny how smiley's can even make the tuffest guys look like sweet pea's
Quote:

PussyFart said:

Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.:smile:

Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.:cool:

P. Cubensis are a tropical species. You could colonize at 70F and fruit at 80F with great results.
:eek:
Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.:naughty:

You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.
:blush:
You want direct/intense 6500K light on a 12/12 schedule for fruiting.:takingnotes:

Optimal temps are mid 70s throughout the whole grow, but anywhere from 65F-80F is acceptable.:thumbup:

Incubation is outdated/uneeded unless temps in the range stated above cannot be kept.:super:

The inside of the jar is always a few degrees warmer than the outside because the mycellium produces heat..mycellium tends to stall at temps above 86F , and contams thrive.
:crazy:
Fruiting at cooler temps tends to produce denser, meatier fruits, while fruiting at higher temps will often produce hollow, less dense stems.
:seriousthankyou:



Mycelium should be exposed to ambient room light from day of inoculation as has been known for many years.  Light is not a pinning trigger until after full colonization and an increase in air is given, and even then it's a secondary pinning trigger.
RR
:grin:

Shake once at about 30% colonization.....no time frame can be put on this, it happens when it happens.:smile:

Please remove the vendors name from your post, and take a moment to read thru the forum rules.:tongue2:

Rules Thread-READ BEFORE POSTING:jackiechanofapproval:




:laugh2:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
Trusted Cultivator
Re: WBS bulk spawn - Inconsistent jars... [Re: cronicr]
    #20209323 - 07/01/14 03:57 AM (10 years, 6 months ago)

:nodofunderstanding:

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OfflinecronicrMFacebook
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Registered: 08/07/11
Posts: 62,905
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Re: WBS bulk spawn - Inconsistent jars... [Re: PussyFart]
    #20209351 - 07/01/14 04:18 AM (10 years, 6 months ago)

happy canada day:hehehe::guiltyascharged:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
Trusted Cultivator
Re: WBS bulk spawn - Inconsistent jars... [Re: cronicr]
    #20209364 - 07/01/14 04:28 AM (10 years, 6 months ago)

I am not Canadian.....but everyday living in the US I wish I was more and more....

90% of all politicians need to be snuffed out....not a terroristic threat, just an educated observation.

Edited by PussyFart (07/01/14 04:29 AM)

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