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Offlinemosby2013
Stranger
Registered: 02/03/14
Posts: 29
Last seen: 6 years, 11 months
Clone to fruit, back to clone = senescence?
    #20200389 - 06/28/14 07:45 PM (10 years, 6 months ago)

Just a question, say I buy a mushroom and clone it in petri dish, then transfer that to grain and did a few G2G transfer, then transferred that to sawdust and fruited them. Then, i get a fruited mushroom and cloned it again in a petri dish, that entire process senescence occurs right? so in theory, the mycelium from that 2nd clone will be weaker? and if this process was to continue indefinitely, eventually it just wouldnt grow?

my question then is, how do mushrooms even exist? surely over the history of the universe mushroom species die out?

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OfflineMarch Hare
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Registered: 06/26/14
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Re: Clone to fruit, back to clone = senescence? [Re: mosby2013]
    #20200417 - 06/28/14 07:50 PM (10 years, 6 months ago)

:facepalm3:

Babies. That's how.


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OfflineMonkeyJesusFresco
am i suspended in agar?
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Registered: 10/09/12
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Re: Clone to fruit, back to clone = senescence? [Re: March Hare]
    #20200501 - 06/28/14 08:09 PM (10 years, 6 months ago)

Quote:

my question then is, how do mushrooms even exist? surely over the history of the universe mushroom species die out?




All the mushroom species that were destine to die out by this time, have.

Cloning is just one method of cultivating or propagating a species. Don't forget about spores!
Or, as mentioned above "babies".

This is a sexual process. Just a quick run-down, one spore germinates and another spore germinates and they hook-up and get it on and then bam! a fruiting strain is born! Then that strain fruits and drops spores and the cycle continues...

In some species, the spores drop, germinate and that brand new YOUNG dna grows out and connects with the old mycellial network and BAM! the new dna gets taken in by the old mycilium and it's rejuvenated...

that's also how you get large acres and acres areas of fungus

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OfflinePussyFart
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Re: Clone to fruit, back to clone = senescence? [Re: mosby2013] * 2
    #20200549 - 06/28/14 08:25 PM (10 years, 6 months ago)

Quote:

mosby2013 said:
my question then is, how do mushrooms even exist?



Because RR said so.

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OfflineMastaBlastar
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Registered: 04/06/13
Posts: 1,069
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Re: Clone to fruit, back to clone = senescence? [Re: PussyFart]
    #20200738 - 06/28/14 09:00 PM (10 years, 6 months ago)

A fruit is a whole new strain and will not reach senescence.  Only a mycelial network will reach senescence.  And as mentioned, to help you understand spores are like sperm, but instead of going to an egg a spore just needs to touch another spore.  And musherooms exist because GOD WANTS YOU TO GET HIGH.


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OfflineMonkeyJesusFresco
am i suspended in agar?
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Re: Clone to fruit, back to clone = senescence? [Re: MastaBlastar]
    #20200786 - 06/28/14 09:08 PM (10 years, 6 months ago)

and to touch on the idea that it is indeed sexual, fungi have lots of mating types, where was we have essentially two: male & female

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Offlinemosby2013
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Registered: 02/03/14
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Re: Clone to fruit, back to clone = senescence? [Re: MonkeyJesusFresco]
    #20200955 - 06/28/14 09:48 PM (10 years, 6 months ago)

So just on senescence of a mycelial network - if its weaker due to too many g2g transfers, wouldnt the mycelium in the fruit also be weaker? (since they are genetically the same)?

So with that logic, is the spores from that fruit also weaker?

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OfflineMonkeyJesusFresco
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Re: Clone to fruit, back to clone = senescence? [Re: mosby2013] * 1
    #20201012 - 06/28/14 10:00 PM (10 years, 6 months ago)

nah the spores from the fruits aren't gonna be weaker, or at least, not like that...

Quick run down of how to properly keep a culture!

1) Germinate spores on agar

2) Transfer from contamination, then Isolate a strain (isolate if desired)

3) Put healthy strain on a culture-slant (in a petri dish/jar or test tube) this is your "Master Culture"

4) Get a grain jar, drop a little piece from your "master culture" into this grain jar

--Put your master culture in the refrigerator.

5) Grain jar colonizes, this is called "Master Grain Jar"

6) Grain 2 grain that jar to a dozen or so others: at this point the myceillium is a couple of weeks old

Now, remember that "master culture" you put in the fridge? The cold slowed the growth, in the fridge, cell division has been for the most part halted. Technically, the agar master culture is younger than your fully colonized grain jars (if only by a week or two)

Everytime you need a "Master Grain Jar" you go back to the fridge and grab a piece of agar from your "master-culture", it's as old as the last time you used it...

hope that makes some sense, you repeat this process and plan ahead and make multiple master cultures or multiple master grain jars and your culture can out live YOU.

Note: Steps 1-3 can be replaced with:

1) Take clone or pin from first flush

2) Transfer away from contamination and isolate if desired

3) Make a "master" culture.

Typically cloned tissue cultures aren't gonna be affected by age (unless the original spawn/culture was old as shit to begin with)

Hope that's helpful/clear, if it's not or I'm mistaken about something, chime in and I'll clarify/correct!

Edited by MonkeyJesusFresco (06/28/14 10:02 PM)

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OfflineMarch Hare
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Registered: 06/26/14
Posts: 315
Last seen: 10 years, 6 months
Re: Clone to fruit, back to clone = senescence? [Re: MonkeyJesusFresco]
    #20201043 - 06/28/14 10:07 PM (10 years, 6 months ago)



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Invisibleavorg
Quixotic
Folding@home Statistics
Registered: 12/02/04
Posts: 1,348
Re: Clone to fruit, back to clone = senescence? [Re: PussyFart]
    #20201110 - 06/28/14 10:21 PM (10 years, 6 months ago)

Quote:

PussyFart said:
Quote:

mosby2013 said:
my question then is, how do mushrooms even exist?



Because RR said so.




:kimjongil:

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OfflineDos Ounce
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Registered: 09/07/12
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Re: Clone to fruit, back to clone = senescence? [Re: avorg]
    #20201238 - 06/28/14 10:53 PM (10 years, 6 months ago)

i'm gettin' in on this thread :yogabunny:

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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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OG Cultivator
Re: Clone to fruit, back to clone = senescence? [Re: Dos Ounce]
    #20201511 - 06/29/14 12:07 AM (10 years, 6 months ago)

Just a note - mushrooms can reproduce sexually and asexually. Awesome little things.


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Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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