|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Asshat331
PM me if you game on PC




Registered: 05/11/14
Posts: 1,159
Last seen: 10 months, 24 days
|
Agar questions
#20197212 - 06/28/14 01:51 AM (10 years, 6 months ago) |
|
|
How do i store inoculated agar? Is it normal to have a layer of congealed agar on the bottom of my flask?
-------------------- Things change fast, but this too shall pass
Better carve it on your forehead or tattoo it on your ass
Cause who can tell, when the clock strikes twelve
If today’s become tomorrow or if it’s all just gone to hell
|
Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
|
|
Inoculated with what? spores of live tissue?
If you are inoculating with spores, you have to make 2 or 3 transfers to make sure is clean..
From live tissue just let it grow to see if is clean..
For storage in the fridge 
You let your agar cool to much, you have to clean your flask.
Edited by Mr. Alien (06/28/14 02:31 AM)
|
Asshat331
PM me if you game on PC




Registered: 05/11/14
Posts: 1,159
Last seen: 10 months, 24 days
|
|
ok thanks a bunch man i used spores just to try out the tek, next big cap i get ima take a live sample from. The fridge doesn't slow it down? i do have them in a ziplock too.
-------------------- Things change fast, but this too shall pass
Better carve it on your forehead or tattoo it on your ass
Cause who can tell, when the clock strikes twelve
If today’s become tomorrow or if it’s all just gone to hell
|
blojo02184
Big Red



Registered: 05/15/13
Posts: 3,527
Loc: Maine
Last seen: 6 months, 22 days
|
|
No need to clean flask unless your ocd. If nocced with spores, leave around somewhere comfortable. Store only cultures in fridge. Unused plates last a couple months sealed in a bag.
|
Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
|
|
What do you mean by no need to clean your flask? i clean my flask everytime i use them with hot water and i don't have obsessive compulsive disorder.
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
|
alien u funny dude i also clean my snapple bottles after i pour with hot water...and I DO have OCD tendencies
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
|
Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
|
|
lol
|
blojo02184
Big Red



Registered: 05/15/13
Posts: 3,527
Loc: Maine
Last seen: 6 months, 22 days
|
|
Its not a necessity to clean after each use.
|
Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
|
|
it doesn't take very much time to clean it , hot water and a few shakes.
|
Asshat331
PM me if you game on PC




Registered: 05/11/14
Posts: 1,159
Last seen: 10 months, 24 days
|
|
Gonna clean it for sure, thanks for all the help guys.
oh and Snapple bottles are great.
-------------------- Things change fast, but this too shall pass
Better carve it on your forehead or tattoo it on your ass
Cause who can tell, when the clock strikes twelve
If today’s become tomorrow or if it’s all just gone to hell
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
|
snapple bottles are indeed great. beer bottles i had break at 4th cycle, but my snapple bottles are running string still at 9 cycles. not sure why, i would have thought the opposite. u prob do not need to clean them, but why not?? I'm doing dishes anyway ( i don't have dishwasher, but if i did, even more reason to just throw it in there). a soft, nutritious medium, just sitting in a solidified puddle at the bottom of a retail glass jar in ur house/apt?!?! thats weird dude, contam city IME. but then again, i don't save my caps for them...i just use foil during PC cycle. i DO have OCD tendencies tho like i said....probably in all the wrong places tho  but if it works for people, don't fix it
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
|
36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
|
|
Definitely clean your flask and anything you use. You can't expect sterility if your shit's not even visibly clean.
Store your inoculated plates on a shelf. Make sure they're wrapped in Parafilm or at least cling wrap.
If you wanna store cultures, slants are really better but you can just let your plate grow out almost to the edge, throw it in a plastic bag then store it in your fridge. Make a transfer to be sure it's clean before you put it on grain. You'll get at least a few months before you have to transfer to avoid senescence.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
|
|