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InvisibleStygianKnight
A Mushroom

Registered: 03/12/12
Posts: 2,717
Trusted Cultivator
Re: Proper SAB use [Re: PussyFart]
    #20200641 - 06/28/14 08:44 PM (10 years, 6 months ago)

You should be watering your ISO down a bit, around 50% ISO not only sticks around longer but is more effective at killing contams than 70 or 91.
Once I started doing Agar it was pretty obvious where my contams were coming from since each little speck grows right where it lands. 
Gloves and tools were the main source, once I started cleaning them extra well, things got better.

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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Re: Proper SAB use [Re: StygianKnight]
    #20200731 - 06/28/14 08:59 PM (10 years, 6 months ago)

Quote:

StygianKnight said:
You should be watering your ISO down a bit, around 50% ISO not only sticks around longer but is more effective at killing contams than 70 or 91.




Not true.....70% is the most effective....google it.

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InvisibleStygianKnight
A Mushroom

Registered: 03/12/12
Posts: 2,717
Trusted Cultivator
Re: Proper SAB use [Re: PussyFart]
    #20200849 - 06/28/14 09:22 PM (10 years, 6 months ago)

Huh, for some reason I had thought it was around 50% but you're right, noted.
CDC Disinfection data

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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Proper SAB use [Re: StygianKnight]
    #20200897 - 06/28/14 09:35 PM (10 years, 6 months ago)

I personally use very very very diluted soap water. 1-2 drop of dawn or other high quality dish soap to about a quart of water sprayed inside my SAB. I don't have a problem with contamination on g2g or petri dish work. It's the technique and practice that are the important part.

NAH is right though 70% is the best at sanitation.

Edit,
PF not NAH :lol:

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Invisiblepiratez
Unflamable
Male
Registered: 04/21/14
Posts: 209
Loc: i dont knoe
Re: Proper SAB use [Re: bodhisatta]
    #20201766 - 06/29/14 01:53 AM (10 years, 6 months ago)

Nice, 70% ISO is the best?

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OfflineConcreteWaves
Male


Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 2 years, 1 month
Re: Proper SAB use [Re: piratez]
    #20208661 - 06/30/14 08:29 PM (10 years, 6 months ago)

About the iso evaporating...could have to do with climate. One person saying it evaps other dude saying it doesnt...everyones a winner :smile: maybe yall just live in different parts of the country. If it evaps too fast, water that shit down a bit.

What i love most about shroomery, gah, i continue to learn and learn even off of little side posts and what not.


--------------------

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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Proper SAB use [Re: ConcreteWaves]
    #20223072 - 07/04/14 09:00 AM (10 years, 6 months ago)

If ypu build your sab right then your climate wont matter it's going to saturate the air inside with fumes but there's enough alcohol still wet on the inner surfaces. And yes we already said 70% is the best tested by numerous health agencies.

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