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Offlinesahadjohanni
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Sterile Attire Question
    #20188810 - 06/26/14 11:21 AM (10 years, 6 months ago)

1) Do you guys think it would be better to wear a hooded Tyvek suit or a nurse style hair cap and tyvek arm sleeves?

2) Do you think it makes that big of a difference when your working with a SAB? The suit is much more expensive but less chance of contams is all that really matters in the end. Thoughts?

3) Can you reuse Tyvek arm sleeves or a Tyvek body suit more than once? Not sure if you can wash them or even spray them down with Lysol at a later date.

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OfflineMonkeyJesusFresco
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20188825 - 06/26/14 11:25 AM (10 years, 6 months ago)

1) wear it if ya got it :shrug: sounds fun

2) the suit you're wearing is outside the box, how is it relevant when you're working inside the box?

3) I guess you could roll 'em up in a jar and PC them :smile:

now, I dunno about anybody else, but I use dish-washing rubber gloves with long sleeves that go up my forearm, and don't have contamination issues inside the SAB


--------------------
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OfflineMonkeyJesusFresco
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Re: Sterile Attire Question [Re: MonkeyJesusFresco]
    #20188832 - 06/26/14 11:30 AM (10 years, 6 months ago)

also, I mean, at home and hobby use, by the time you get the tyvek suit on, it won't be sterile anymore.

I call overkill. Maybe a hair net, but the air is what you need to be concerned about. still air or filtered air from a .....whateverthefuckthosethingsarecalled....

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Offlinealex591955
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Re: Sterile Attire Question [Re: MonkeyJesusFresco]
    #20188842 - 06/26/14 11:32 AM (10 years, 6 months ago)

1. I use to do this. I felt pretty cool ha. I had a dust mask to and would use rubber gloves. The only thing is. You build up a sweat. Like shit gets hot.

2. :whathesaid:

3. the idea is to have a barrier from your skin. So tyvek sleeves, Long sleeves, dishwashing gloves. That all works out pretty well. i think if you gave it a light mist it wouldnt hurt.

get yourself a mister with either bleach water or Iso 70%


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My Current Grow


Edited by alex591955 (06/26/14 11:32 AM)

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Invisibleindocult
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Re: Sterile Attire Question [Re: alex591955]
    #20188846 - 06/26/14 11:33 AM (10 years, 6 months ago)

Tyvek sleeves are a lot cheaper to keep a steady supply of

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Offlinesahadjohanni
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Re: Sterile Attire Question [Re: MonkeyJesusFresco]
    #20188854 - 06/26/14 11:35 AM (10 years, 6 months ago)

Quote:

MonkeyJesusFresco said:
the suit you're wearing is outside the box, how is it relevant when you're working inside the box?



The needle has to come out and get steralized a bunch of times. While it is out you could conceivably shed skin/hair/contams if not properly protected. I have a 5" LC needle and will need to flame steralize it a total of 24 times. Technically that is a lot of time spent outside of the box.

Prior to finding the Tyvek body suit I planned to use Nirite medical gloves, Tyvek arm sleeves, surgical grade face mask, and a medical hair cover. Am I being too paranoid for SAB work? Is it overkill?

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Offlinelateforthafuture
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20188855 - 06/26/14 11:36 AM (10 years, 6 months ago)

I wear a surgical mask, latex gloves and just wipe my forarems and hands and gloves down really well with alcohol each and every time I enter the still air box. I don't wear any tyvek sleeves, suit or hair restraint and I don't have any issues.


--------------------
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Offlinealex591955
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20188861 - 06/26/14 11:39 AM (10 years, 6 months ago)

I think the suit would be over kill. You start sweating pretty bad and you wont wanna use it again.

What you have listed sounds good to me. Might be a bit overkill but hey, better safe then sorry.
T


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Offlinesahadjohanni
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Re: Sterile Attire Question [Re: indocult]
    #20188863 - 06/26/14 11:40 AM (10 years, 6 months ago)

Quote:

MonkeyJesusFresco said:
also, I mean, at home and hobby use, by the time you get the tyvek suit on, it won't be sterile anymore.



An extremely good point. Screw the suit :grin:

Quote:

alex591955 said:
The only thing is. You build up a sweat. Like shit gets hot.



Another great point. I hadn't thought of that :wink:


Quote:

indocult said:
Tyvek sleeves are a lot cheaper to keep a steady supply of



LOL very true  :biggrin:

EDIT

Quote:

alex591955 said:
What you have listed sounds good to me. Might be a bit overkill but hey, better safe then sorry.



That is my line of thinking. Doesn't hurt being prepared and I don't trust a single molecule of air in this house :uhoh:.

Edited by sahadjohanni (06/26/14 11:42 AM)

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OfflineMonkeyJesusFresco
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20188869 - 06/26/14 11:43 AM (10 years, 6 months ago)

what prevents flame sterilizing your needl INSIDE the box?
still air, got'cha:crazy2:

Edited by MonkeyJesusFresco (06/26/14 03:22 PM)

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Offlinealex591955
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Re: Sterile Attire Question [Re: MonkeyJesusFresco]
    #20188960 - 06/26/14 12:15 PM (10 years, 6 months ago)

Quote:

MonkeyJesusFresco said:
what prevents flame sterilizing your needl INSIDE the box?



Well personally i use a blue blow torche. it wouldnt even fit in my SOB. The second thing is. If you say, use ;lysol and spray it inside your tub, its gonna blow up if you light something inside most likely.  I keep a wet paper towel of iso inside and wipe my arm and gloves everytime i go in.


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OfflineMonkeyJesusFresco
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Re: Sterile Attire Question [Re: alex591955]
    #20189020 - 06/26/14 12:28 PM (10 years, 6 months ago)

meh, iso can carry contaminate spores too (apparently)

this kind of shit is why I moved away from syringe work :shrug:

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Re: Sterile Attire Question [Re: alex591955]
    #20189039 - 06/26/14 12:33 PM (10 years, 6 months ago)

Yeah you're supposed to keep your alcohol lamp outside the sab .

you aren't being overkill, op.

I got a new place one time, and before there I just wore gloves and surgeons mask, but when I started at the new place my contam went through the roof! Older house must have had a much higher spore count inside.

Anyway I got tyvek sleeves and hair net and my contam rates went back down to almost zero.

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Invisiblenucleophile
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Re: Sterile Attire Question [Re: indocult]
    #20189321 - 06/26/14 01:30 PM (10 years, 6 months ago)

The sleeves are very much reusable, good tyvek sleeves can be PC'd but there's really no point to it sinc they're exposed to air. Just wipe them with alcohol or spray with Lysol, they're mainly to keep skin and stuff on your skin contained. As far as the other things are concerned, just make sure you're clean and you can wear a Surgical Facemask if you want (not a cheapy dust mask, make sure it's rated for small particle size), wear some clean clothes. It isn't as important as technique in the box but it does help. If you can find gloves with longer sleeves, those are great since the tyvek cuffs tend to ride up and expose your wrists.

Even if there isn't anything explosive in the box, you shouldn't flame inside because it creates air currents, which is a Bad Thing. I've done it and it usually works okay but it's not good practice.

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Offlinesahadjohanni
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Re: Sterile Attire Question [Re: nucleophile]
    #20189645 - 06/26/14 02:58 PM (10 years, 6 months ago)

Quote:

MonkeyJesusFresco said:
what prevents flame sterilizing your needl INSIDE the box?



The entire box will probably go up if I put a flame in there. Lots of Lysol and ISO 80%.


Quote:

indocult said:
You aren't being overkill, op.



Great story and good to hear it. Been a bit worried about prepping too much.


Quote:

nucleophile said:
The sleeves are very much reusable. Just wipe them with alcohol or spray with Lysol, they're mainly to keep skin and stuff on your skin contained...

just make sure you're clean and you can wear a Surgical Facemask if you want (not a cheapy dust mask, make sure it's rated for small particle size)...

If you can find gloves with longer sleeves, those are great since the tyvek cuffs tend to ride up and expose your wrists.



Good to know they are reusable. I really don't want to buy extras if I don't have to. Thank you for the tips on the gloves. I'll have to reassess and see what I can find.

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Offlinesahadjohanni
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20189675 - 06/26/14 03:06 PM (10 years, 6 months ago)

What am I looking for in a face mask? I'm currently looking at purchasing these. They have a BFE (bacteria) and PFE (particles) of 98%. The Fluid Resistance is low end (80 mmHg). Do I need to track down some N95 face masks or should the above cut it? N95 is the best there is, capable of protecting you from airborne diseases like TB and influenza.

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Re: Sterile Attire Question [Re: sahadjohanni]
    #20189685 - 06/26/14 03:08 PM (10 years, 6 months ago)

as long as it's a surgical mask and not a dust mask it's fine


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OfflineMonkeyJesusFresco
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20189687 - 06/26/14 03:08 PM (10 years, 6 months ago)

ya might be able to eliminate the lysol with 1:10 (bleach:water) spray bottle

the only reason I've been blasting the SAB with lysol(not that that does anything, other than make me feel better) is cause I ran out of bleach :shrug:

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Re: Sterile Attire Question [Re: MonkeyJesusFresco]
    #20189723 - 06/26/14 03:20 PM (10 years, 6 months ago)

I wear the top half of tyvek suit, high rise dish-washing gloves, surgical mask, and a bandana on my head. I may get new tyvek sleeves this weekend because mine are starting to become mesh.

I use diluted Dr. Bronners Magical soap in the SAB, and alcohol on my jars.

Flame sterilization is done outside the SAB to prevent defeating the purpose of a SAB.

My SAB


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Offlinesahadjohanni
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Re: Sterile Attire Question [Re: SpitballJedi]
    #20189781 - 06/26/14 03:37 PM (10 years, 6 months ago)

Quote:

MonkeyJesusFresco said:
ya might be able to eliminate the lysol with 1:10 (bleach:water) spray bottle.



I'm recently disenchanted with Lysol. I had no idea it was so weak. I've been using these wipes forever and just noticed recently that it takes 10 minutes afterwards for the actual bacteria killing action to take place. I'll try out the bleach approach and see if my lungs can handle it.


Quote:

SpitballJedi said:
My SAB



Thanks for the link. In your article you mention covering up the arm holes with cling-wrap. Is this so you don't have to re-sterilize the SAB when you come back after a short break or?

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Re: Sterile Attire Question [Re: sahadjohanni]
    #20189793 - 06/26/14 03:41 PM (10 years, 6 months ago)

It just helps keep stuff from floating in when I step away. An SAB is not meant to be sterile.

Lysol is useless and I hate bleach. Neither are necessary for working in a SAB.


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Offlinesahadjohanni
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20189817 - 06/26/14 03:47 PM (10 years, 6 months ago)

What do you guys use to cover your hair. Would a hat or shower cap do or should I look into buying those surgical hats (bouffants)?

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Re: Sterile Attire Question [Re: sahadjohanni]
    #20189827 - 06/26/14 03:50 PM (10 years, 6 months ago)

I use a clean bandana


Edited by SpitballJedi (06/26/14 03:52 PM)

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OfflineMonkeyJesusFresco
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Re: Sterile Attire Question [Re: SpitballJedi]
    #20189872 - 06/26/14 04:01 PM (10 years, 6 months ago)

Quote:

SpitballJedi said:
It just helps keep stuff from floating in when I step away. An SAB is not meant to be sterile.

Lysol is useless and I hate bleach. Neither are necessary for working in a SAB.




:whathesaid:

unless you're just doing to feel better, that's what I do :shrug:
same with the hair net, I imagine

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Offlinesahadjohanni
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Re: Sterile Attire Question [Re: SpitballJedi]
    #20189885 - 06/26/14 04:05 PM (10 years, 6 months ago)

Quote:

SpitballJedi said:
I use a clean bandana



That is very affordable and a great idea. I plan on sweating in that closet so it will be a welcome replacement for the bouffant :tongue2:. Also thanks for the picture. I was trying to imagine it on there lol. I've never worn one before :confused:.

One last question about the gloves. I'm having trouble finding any surgical-like gloves that go to the wrist. Everything I'm finding is thick plastic (inhibits dexterity). Someone mentioned the sleeves tend to inch their way up and expose the wrist. I could use duct tape to attach the two together but fear ripping the tape off will damage the tyvek sleeve. Those things are just too darn expensive :mad2:. Anybody have an experience with doing this?

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OfflineKizzle
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Re: Sterile Attire Question [Re: SpitballJedi]
    #20189899 - 06/26/14 04:08 PM (10 years, 6 months ago)

I don't see any point in covering your hair if you're using a box. It's like a giant hairnet that covers the entire room, except for your arms and hands.


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Offlinealex591955
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20189988 - 06/26/14 04:26 PM (10 years, 6 months ago)

Quote:

sahadjohanni said:
Quote:

SpitballJedi said:
I use a clean bandana



That is very affordable and a great idea. I plan on sweating in that closet so it will be a welcome replacement for the bouffant :tongue2:. Also thanks for the picture. I was trying to imagine it on there lol. I've never worn one before :confused:.

One last question about the gloves. I'm having trouble finding any surgical-like gloves that go to the wrist. Everything I'm finding is thick plastic (inhibits dexterity). Someone mentioned the sleeves tend to inch their way up and expose the wrist. I could use duct tape to attach the two together but fear ripping the tape off will damage the tyvek sleeve. Those things are just too darn expensive :mad2:. Anybody have an experience with doing this?




rubber bands.


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Re: Sterile Attire Question [Re: alex591955]
    #20190017 - 06/26/14 04:34 PM (10 years, 6 months ago)

walmart has dish washing gloves that come up pretty far. They're thicker than surgical gloves, but dexterity is still good. It's what I use.

Open the package and try them on. The first ones I got were big and loose. The ones that fit a little tighter work best for me.


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OfflineDirtyTomFlint
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20190059 - 06/26/14 04:42 PM (10 years, 6 months ago)

Get the yellow suit they wear in Breaking Bad.


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Re: Sterile Attire Question [Re: DirtyTomFlint]
    #20190892 - 06/26/14 07:42 PM (10 years, 6 months ago)

Nitrile gloves are my favorite. the thicker gloves make all your movement restricted and clumsy

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Re: Sterile Attire Question [Re: indocult]
    #20191104 - 06/26/14 08:13 PM (10 years, 6 months ago)

I just cut thumb holes in my tyvek sleeves that way my latex gloves cover the sleeves and stay that way, works great.

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OfflineRogerRabbitM
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20191414 - 06/26/14 09:10 PM (10 years, 6 months ago)

Quote:

sahadjohanni said:
What am I looking for in a face mask? I'm currently looking at purchasing these. They have a BFE (bacteria) and PFE (particles) of 98%. The Fluid Resistance is low end (80 mmHg). Do I need to track down some N95 face masks or should the above cut it? N95 is the best there is, capable of protecting you from airborne diseases like TB and influenza.




N95 is worthless for mycology.  The masks you linked are OK.  We're not trying to protect ourselves from our mushroom cultures.  We're trying to protect them from us.  Use surgical gloves and masks.  Surgical masks are designed to filter exhaled bacteria, not inhaled solids.

It doesn't matter if tyvek or cotton sleeves are sterile or not.  They just need to be clean.  The purpose is to capture dead skin cells which flake off your arms by the millions, to prevent them swirling into your sterile media.
RR


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Re: Sterile Attire Question [Re: RogerRabbit]
    #20191426 - 06/26/14 09:12 PM (10 years, 6 months ago)

:jokerclap:

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Offlinesahadjohanni
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Re: Sterile Attire Question [Re: RogerRabbit]
    #20193636 - 06/27/14 10:49 AM (10 years, 6 months ago)

Quote:

alex591955 said:
rubber bands.



:eek: It's so obvious! Thank you :dancer:


Quote:

indocult said:
Nitrile gloves are my favorite. the thicker gloves make all your movement restricted and clumsy



I've always had dexterity issues with dish washing gloves so I'll be going with Nitrile gloves. Just hope they fit my freakishly large hands :uhoh:.


Quote:

RogerRabbit said:
N95 is worthless for mycology.
It doesn't matter if tyvek or cotton sleeves are sterile or not.  They just need to be clean.



Very good to know. Thank you! :biggrin:

Edited by sahadjohanni (06/27/14 11:01 AM)

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Offlinesahadjohanni
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20193682 - 06/27/14 11:02 AM (10 years, 6 months ago)

When it comes to gloves does it matter if they are exam or surgical? How about sterile vs non-sterile?

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OfflineMonkeyJesusFresco
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20193699 - 06/27/14 11:06 AM (10 years, 6 months ago)

by the time it gets onto your hands and into your box they're not gonna be sterile,

like someone who isn't me above said, it's to keep your nasty-ass skin flakes off yo shit.

they nitrile gloves are resistant to things like alcohol, just say'n. the purple ones.

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Offlinesahadjohanni
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Re: Sterile Attire Question [Re: MonkeyJesusFresco]
    #20193733 - 06/27/14 11:16 AM (10 years, 6 months ago)

Quote:

MonkeyJesusFresco said:
like someone who isn't me above said, it's to keep your nasty-ass skin flakes off yo shit.



I figured as much. RR specifically said surgical gloves and masks. I just noticed the ones I'm about to order are exam gloves so I paused. But flakes are flakes so these should do just fine :biggrin:. Working with LC has me super paranoid :uhoh:.

Alright folks, I'm putting my orders in for the supplies. Thank you all very much for your feedback, advise, and time :bow2:.

Edited by sahadjohanni (06/27/14 01:57 PM)

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OfflinePussyFart
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20193747 - 06/27/14 11:18 AM (10 years, 6 months ago)

Quote:

sahadjohanni said:
Working with LC has me super paranoid :uhoh:.



Understandable.....working with the riskiest media would have me on my toes as well.

This is why I moved away from LC and into agar.....

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Offlinesahadjohanni
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Re: Sterile Attire Question [Re: PussyFart]
    #20209927 - 07/01/14 09:36 AM (10 years, 6 months ago)

So I'll be working with a SAB in a small walk-in closet. The floor is carpeted. Should I go the extra step of laying down 10mm thick painters plastic to protect from carpet contams? Or is that pretty pointless since I'll be working inside a SAB?

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Re: Sterile Attire Question [Re: sahadjohanni]
    #20209976 - 07/01/14 09:54 AM (10 years, 6 months ago)

If you have it do it.  carpet holds a lot of spores and you could have some  issues.

I wouldn't spend the extra $ tho if you don't have it

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Offlinesahadjohanni
Budding Mycologist


Registered: 03/04/13
Posts: 55
Last seen: 10 years, 5 months
Re: Sterile Attire Question [Re: sahadjohanni]
    #20210015 - 07/01/14 10:10 AM (10 years, 6 months ago)

I don't own any. The 10mil stuff is expensive now that I'm looking. Would something like this work? I would have to lay it down in strips. I'm not sure if that really helps since walking on it probably causes carpet contams to rise onto the plastic. If not this sort of stuff, should I be looking at plastic furniture covers? That way it would be one solid mass :rolleyes:.

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