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InvisibleSpitballJedi
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20189793 - 06/26/14 03:41 PM (10 years, 6 months ago)

It just helps keep stuff from floating in when I step away. An SAB is not meant to be sterile.

Lysol is useless and I hate bleach. Neither are necessary for working in a SAB.


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Offlinesahadjohanni
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Registered: 03/04/13
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20189817 - 06/26/14 03:47 PM (10 years, 6 months ago)

What do you guys use to cover your hair. Would a hat or shower cap do or should I look into buying those surgical hats (bouffants)?

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InvisibleSpitballJedi
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20189827 - 06/26/14 03:50 PM (10 years, 6 months ago)

I use a clean bandana


Edited by SpitballJedi (06/26/14 03:52 PM)

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OfflineMonkeyJesusFresco
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Re: Sterile Attire Question [Re: SpitballJedi]
    #20189872 - 06/26/14 04:01 PM (10 years, 6 months ago)

Quote:

SpitballJedi said:
It just helps keep stuff from floating in when I step away. An SAB is not meant to be sterile.

Lysol is useless and I hate bleach. Neither are necessary for working in a SAB.




:whathesaid:

unless you're just doing to feel better, that's what I do :shrug:
same with the hair net, I imagine

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Offlinesahadjohanni
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Re: Sterile Attire Question [Re: SpitballJedi]
    #20189885 - 06/26/14 04:05 PM (10 years, 6 months ago)

Quote:

SpitballJedi said:
I use a clean bandana



That is very affordable and a great idea. I plan on sweating in that closet so it will be a welcome replacement for the bouffant :tongue2:. Also thanks for the picture. I was trying to imagine it on there lol. I've never worn one before :confused:.

One last question about the gloves. I'm having trouble finding any surgical-like gloves that go to the wrist. Everything I'm finding is thick plastic (inhibits dexterity). Someone mentioned the sleeves tend to inch their way up and expose the wrist. I could use duct tape to attach the two together but fear ripping the tape off will damage the tyvek sleeve. Those things are just too darn expensive :mad2:. Anybody have an experience with doing this?

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OfflineKizzle
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Re: Sterile Attire Question [Re: SpitballJedi]
    #20189899 - 06/26/14 04:08 PM (10 years, 6 months ago)

I don't see any point in covering your hair if you're using a box. It's like a giant hairnet that covers the entire room, except for your arms and hands.


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Offlinealex591955
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20189988 - 06/26/14 04:26 PM (10 years, 6 months ago)

Quote:

sahadjohanni said:
Quote:

SpitballJedi said:
I use a clean bandana



That is very affordable and a great idea. I plan on sweating in that closet so it will be a welcome replacement for the bouffant :tongue2:. Also thanks for the picture. I was trying to imagine it on there lol. I've never worn one before :confused:.

One last question about the gloves. I'm having trouble finding any surgical-like gloves that go to the wrist. Everything I'm finding is thick plastic (inhibits dexterity). Someone mentioned the sleeves tend to inch their way up and expose the wrist. I could use duct tape to attach the two together but fear ripping the tape off will damage the tyvek sleeve. Those things are just too darn expensive :mad2:. Anybody have an experience with doing this?




rubber bands.


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InvisibleSpitballJedi
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Re: Sterile Attire Question [Re: alex591955]
    #20190017 - 06/26/14 04:34 PM (10 years, 6 months ago)

walmart has dish washing gloves that come up pretty far. They're thicker than surgical gloves, but dexterity is still good. It's what I use.

Open the package and try them on. The first ones I got were big and loose. The ones that fit a little tighter work best for me.


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A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum

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OfflineDirtyTomFlint
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20190059 - 06/26/14 04:42 PM (10 years, 6 months ago)

Get the yellow suit they wear in Breaking Bad.


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Know Your Body, Know Your Mind, Know Your Substance, Know Your Source

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Invisibleindocult
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Re: Sterile Attire Question [Re: DirtyTomFlint]
    #20190892 - 06/26/14 07:42 PM (10 years, 6 months ago)

Nitrile gloves are my favorite. the thicker gloves make all your movement restricted and clumsy

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Offlineshaymisco
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Re: Sterile Attire Question [Re: indocult]
    #20191104 - 06/26/14 08:13 PM (10 years, 6 months ago)

I just cut thumb holes in my tyvek sleeves that way my latex gloves cover the sleeves and stay that way, works great.

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OfflineRogerRabbitM
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20191414 - 06/26/14 09:10 PM (10 years, 6 months ago)

Quote:

sahadjohanni said:
What am I looking for in a face mask? I'm currently looking at purchasing these. They have a BFE (bacteria) and PFE (particles) of 98%. The Fluid Resistance is low end (80 mmHg). Do I need to track down some N95 face masks or should the above cut it? N95 is the best there is, capable of protecting you from airborne diseases like TB and influenza.




N95 is worthless for mycology.  The masks you linked are OK.  We're not trying to protect ourselves from our mushroom cultures.  We're trying to protect them from us.  Use surgical gloves and masks.  Surgical masks are designed to filter exhaled bacteria, not inhaled solids.

It doesn't matter if tyvek or cotton sleeves are sterile or not.  They just need to be clean.  The purpose is to capture dead skin cells which flake off your arms by the millions, to prevent them swirling into your sterile media.
RR


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OfflineMonkeyJesusFresco
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Re: Sterile Attire Question [Re: RogerRabbit]
    #20191426 - 06/26/14 09:12 PM (10 years, 6 months ago)

:jokerclap:

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Offlinesahadjohanni
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Registered: 03/04/13
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Re: Sterile Attire Question [Re: RogerRabbit]
    #20193636 - 06/27/14 10:49 AM (10 years, 6 months ago)

Quote:

alex591955 said:
rubber bands.



:eek: It's so obvious! Thank you :dancer:


Quote:

indocult said:
Nitrile gloves are my favorite. the thicker gloves make all your movement restricted and clumsy



I've always had dexterity issues with dish washing gloves so I'll be going with Nitrile gloves. Just hope they fit my freakishly large hands :uhoh:.


Quote:

RogerRabbit said:
N95 is worthless for mycology.
It doesn't matter if tyvek or cotton sleeves are sterile or not.  They just need to be clean.



Very good to know. Thank you! :biggrin:

Edited by sahadjohanni (06/27/14 11:01 AM)

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Offlinesahadjohanni
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20193682 - 06/27/14 11:02 AM (10 years, 6 months ago)

When it comes to gloves does it matter if they are exam or surgical? How about sterile vs non-sterile?

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OfflineMonkeyJesusFresco
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20193699 - 06/27/14 11:06 AM (10 years, 6 months ago)

by the time it gets onto your hands and into your box they're not gonna be sterile,

like someone who isn't me above said, it's to keep your nasty-ass skin flakes off yo shit.

they nitrile gloves are resistant to things like alcohol, just say'n. the purple ones.

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Offlinesahadjohanni
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Re: Sterile Attire Question [Re: MonkeyJesusFresco]
    #20193733 - 06/27/14 11:16 AM (10 years, 6 months ago)

Quote:

MonkeyJesusFresco said:
like someone who isn't me above said, it's to keep your nasty-ass skin flakes off yo shit.



I figured as much. RR specifically said surgical gloves and masks. I just noticed the ones I'm about to order are exam gloves so I paused. But flakes are flakes so these should do just fine :biggrin:. Working with LC has me super paranoid :uhoh:.

Alright folks, I'm putting my orders in for the supplies. Thank you all very much for your feedback, advise, and time :bow2:.

Edited by sahadjohanni (06/27/14 01:57 PM)

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OfflinePussyFart
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20193747 - 06/27/14 11:18 AM (10 years, 6 months ago)

Quote:

sahadjohanni said:
Working with LC has me super paranoid :uhoh:.



Understandable.....working with the riskiest media would have me on my toes as well.

This is why I moved away from LC and into agar.....

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Offlinesahadjohanni
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Re: Sterile Attire Question [Re: PussyFart]
    #20209927 - 07/01/14 09:36 AM (10 years, 6 months ago)

So I'll be working with a SAB in a small walk-in closet. The floor is carpeted. Should I go the extra step of laying down 10mm thick painters plastic to protect from carpet contams? Or is that pretty pointless since I'll be working inside a SAB?

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Invisibleindocult
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Re: Sterile Attire Question [Re: sahadjohanni]
    #20209976 - 07/01/14 09:54 AM (10 years, 6 months ago)

If you have it do it.  carpet holds a lot of spores and you could have some  issues.

I wouldn't spend the extra $ tho if you don't have it

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Shop: Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale


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