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sahadjohanni
Budding Mycologist


Registered: 03/04/13
Posts: 55
Last seen: 10 years, 5 months
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Sterile Attire Question
#20188810 - 06/26/14 11:21 AM (10 years, 6 months ago) |
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1) Do you guys think it would be better to wear a hooded Tyvek suit or a nurse style hair cap and tyvek arm sleeves?
2) Do you think it makes that big of a difference when your working with a SAB? The suit is much more expensive but less chance of contams is all that really matters in the end. Thoughts?
3) Can you reuse Tyvek arm sleeves or a Tyvek body suit more than once? Not sure if you can wash them or even spray them down with Lysol at a later date.
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,308
Loc: South East USA
Last seen: 24 days, 18 hours
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1) wear it if ya got it sounds fun
2) the suit you're wearing is outside the box, how is it relevant when you're working inside the box?
3) I guess you could roll 'em up in a jar and PC them 
now, I dunno about anybody else, but I use dish-washing rubber gloves with long sleeves that go up my forearm, and don't have contamination issues inside the SAB
-------------------- LAGM v 2.024
- endo cabendo
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,308
Loc: South East USA
Last seen: 24 days, 18 hours
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also, I mean, at home and hobby use, by the time you get the tyvek suit on, it won't be sterile anymore.
I call overkill. Maybe a hair net, but the air is what you need to be concerned about. still air or filtered air from a .....whateverthefuckthosethingsarecalled....
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alex591955
i feel funny



Registered: 02/17/10
Posts: 672
Last seen: 4 years, 2 months
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1. I use to do this. I felt pretty cool ha. I had a dust mask to and would use rubber gloves. The only thing is. You build up a sweat. Like shit gets hot.
2. 
3. the idea is to have a barrier from your skin. So tyvek sleeves, Long sleeves, dishwashing gloves. That all works out pretty well. i think if you gave it a light mist it wouldnt hurt.
get yourself a mister with either bleach water or Iso 70%
-------------------- My Current Grow
Edited by alex591955 (06/26/14 11:32 AM)
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indocult
Dr


Registered: 07/12/09
Posts: 1,400
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Re: Sterile Attire Question [Re: alex591955]
#20188846 - 06/26/14 11:33 AM (10 years, 6 months ago) |
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Tyvek sleeves are a lot cheaper to keep a steady supply of
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sahadjohanni
Budding Mycologist


Registered: 03/04/13
Posts: 55
Last seen: 10 years, 5 months
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Quote:
MonkeyJesusFresco said: the suit you're wearing is outside the box, how is it relevant when you're working inside the box?
The needle has to come out and get steralized a bunch of times. While it is out you could conceivably shed skin/hair/contams if not properly protected. I have a 5" LC needle and will need to flame steralize it a total of 24 times. Technically that is a lot of time spent outside of the box.
Prior to finding the Tyvek body suit I planned to use Nirite medical gloves, Tyvek arm sleeves, surgical grade face mask, and a medical hair cover. Am I being too paranoid for SAB work? Is it overkill?
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lateforthafuture
fanatic


Registered: 03/26/07
Posts: 471
Last seen: 4 years, 3 months
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I wear a surgical mask, latex gloves and just wipe my forarems and hands and gloves down really well with alcohol each and every time I enter the still air box. I don't wear any tyvek sleeves, suit or hair restraint and I don't have any issues.
-------------------- "Not what could have been, or what should have been, but what will be" -Self
 
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alex591955
i feel funny



Registered: 02/17/10
Posts: 672
Last seen: 4 years, 2 months
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I think the suit would be over kill. You start sweating pretty bad and you wont wanna use it again.
What you have listed sounds good to me. Might be a bit overkill but hey, better safe then sorry. T
-------------------- My Current Grow
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sahadjohanni
Budding Mycologist


Registered: 03/04/13
Posts: 55
Last seen: 10 years, 5 months
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Re: Sterile Attire Question [Re: indocult]
#20188863 - 06/26/14 11:40 AM (10 years, 6 months ago) |
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Quote:
MonkeyJesusFresco said: also, I mean, at home and hobby use, by the time you get the tyvek suit on, it won't be sterile anymore.
An extremely good point. Screw the suit 
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alex591955 said: The only thing is. You build up a sweat. Like shit gets hot.
Another great point. I hadn't thought of that 
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indocult said: Tyvek sleeves are a lot cheaper to keep a steady supply of
LOL very true 
EDIT
Quote:
alex591955 said: What you have listed sounds good to me. Might be a bit overkill but hey, better safe then sorry.
That is my line of thinking. Doesn't hurt being prepared and I don't trust a single molecule of air in this house .
Edited by sahadjohanni (06/26/14 11:42 AM)
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,308
Loc: South East USA
Last seen: 24 days, 18 hours
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what prevents flame sterilizing your needl INSIDE the box? still air, got'cha
Edited by MonkeyJesusFresco (06/26/14 03:22 PM)
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alex591955
i feel funny



Registered: 02/17/10
Posts: 672
Last seen: 4 years, 2 months
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Quote:
MonkeyJesusFresco said: what prevents flame sterilizing your needl INSIDE the box?
Well personally i use a blue blow torche. it wouldnt even fit in my SOB. The second thing is. If you say, use ;lysol and spray it inside your tub, its gonna blow up if you light something inside most likely. I keep a wet paper towel of iso inside and wipe my arm and gloves everytime i go in.
-------------------- My Current Grow
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,308
Loc: South East USA
Last seen: 24 days, 18 hours
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Re: Sterile Attire Question [Re: alex591955]
#20189020 - 06/26/14 12:28 PM (10 years, 6 months ago) |
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meh, iso can carry contaminate spores too (apparently)
this kind of shit is why I moved away from syringe work
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indocult
Dr


Registered: 07/12/09
Posts: 1,400
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Re: Sterile Attire Question [Re: alex591955]
#20189039 - 06/26/14 12:33 PM (10 years, 6 months ago) |
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Yeah you're supposed to keep your alcohol lamp outside the sab .
you aren't being overkill, op.
I got a new place one time, and before there I just wore gloves and surgeons mask, but when I started at the new place my contam went through the roof! Older house must have had a much higher spore count inside.
Anyway I got tyvek sleeves and hair net and my contam rates went back down to almost zero.
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nucleophile
international cowboy chemist


Registered: 04/28/13
Posts: 299
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Re: Sterile Attire Question [Re: indocult]
#20189321 - 06/26/14 01:30 PM (10 years, 6 months ago) |
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The sleeves are very much reusable, good tyvek sleeves can be PC'd but there's really no point to it sinc they're exposed to air. Just wipe them with alcohol or spray with Lysol, they're mainly to keep skin and stuff on your skin contained. As far as the other things are concerned, just make sure you're clean and you can wear a Surgical Facemask if you want (not a cheapy dust mask, make sure it's rated for small particle size), wear some clean clothes. It isn't as important as technique in the box but it does help. If you can find gloves with longer sleeves, those are great since the tyvek cuffs tend to ride up and expose your wrists.
Even if there isn't anything explosive in the box, you shouldn't flame inside because it creates air currents, which is a Bad Thing. I've done it and it usually works okay but it's not good practice.
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sahadjohanni
Budding Mycologist


Registered: 03/04/13
Posts: 55
Last seen: 10 years, 5 months
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Re: Sterile Attire Question [Re: nucleophile]
#20189645 - 06/26/14 02:58 PM (10 years, 6 months ago) |
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Quote:
MonkeyJesusFresco said: what prevents flame sterilizing your needl INSIDE the box?
The entire box will probably go up if I put a flame in there. Lots of Lysol and ISO 80%.
Quote:
indocult said: You aren't being overkill, op.
Great story and good to hear it. Been a bit worried about prepping too much.
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nucleophile said: The sleeves are very much reusable. Just wipe them with alcohol or spray with Lysol, they're mainly to keep skin and stuff on your skin contained...
just make sure you're clean and you can wear a Surgical Facemask if you want (not a cheapy dust mask, make sure it's rated for small particle size)...
If you can find gloves with longer sleeves, those are great since the tyvek cuffs tend to ride up and expose your wrists.
Good to know they are reusable. I really don't want to buy extras if I don't have to. Thank you for the tips on the gloves. I'll have to reassess and see what I can find.
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sahadjohanni
Budding Mycologist


Registered: 03/04/13
Posts: 55
Last seen: 10 years, 5 months
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What am I looking for in a face mask? I'm currently looking at purchasing these. They have a BFE (bacteria) and PFE (particles) of 98%. The Fluid Resistance is low end (80 mmHg). Do I need to track down some N95 face masks or should the above cut it? N95 is the best there is, capable of protecting you from airborne diseases like TB and influenza.
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cronicr



Registered: 08/07/11
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Loc: Van Isle
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as long as it's a surgical mask and not a dust mask it's fine
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,308
Loc: South East USA
Last seen: 24 days, 18 hours
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ya might be able to eliminate the lysol with 1:10 (bleach:water) spray bottle
the only reason I've been blasting the SAB with lysol(not that that does anything, other than make me feel better) is cause I ran out of bleach
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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I wear the top half of tyvek suit, high rise dish-washing gloves, surgical mask, and a bandana on my head. I may get new tyvek sleeves this weekend because mine are starting to become mesh.
I use diluted Dr. Bronners Magical soap in the SAB, and alcohol on my jars.
Flame sterilization is done outside the SAB to prevent defeating the purpose of a SAB.
My SAB
-------------------- The Basics
A little civility goes a long way
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sahadjohanni
Budding Mycologist


Registered: 03/04/13
Posts: 55
Last seen: 10 years, 5 months
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Quote:
MonkeyJesusFresco said: ya might be able to eliminate the lysol with 1:10 (bleach:water) spray bottle.
I'm recently disenchanted with Lysol. I had no idea it was so weak. I've been using these wipes forever and just noticed recently that it takes 10 minutes afterwards for the actual bacteria killing action to take place. I'll try out the bleach approach and see if my lungs can handle it.
Quote:
SpitballJedi said: My SAB
Thanks for the link. In your article you mention covering up the arm holes with cling-wrap. Is this so you don't have to re-sterilize the SAB when you come back after a short break or?
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