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blueconfusion
Strangest


Registered: 12/14/12
Posts: 1,727
Last seen: 15 hours, 46 minutes
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#20140793 - 06/16/14 05:49 PM (9 years, 8 months ago) |
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I've evolved my tek quite a bit since I wrote this.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#20141116 - 06/16/14 06:58 PM (9 years, 8 months ago) |
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Quote:
blueconfusion said:
I've evolved my tek quite a bit since I wrote this.
whats up blue!!
what kinda stuff u doing now compared to the tek?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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ranonar
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Registered: 07/27/06
Posts: 65
Last seen: 9 years, 8 months
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Quote:
to prepare a LC, you whip up some water and nutrients add spores/mycelium and...TA-DA! LC.
True. And a good pointer to the most risky technique! Spores should never be germinated in a liquid. Mycelium should almost never not be grown in a liquid. Ideally the liquid phase should only exist for the purpose of inoculation. It should be made from colonized agar or BRF/verm or whole grain spawn. That is the liquid inoculant (LI)
You may inoculate with a liquid but do the growing on something solid if you want to keep te contam risk down.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Just say NO to LC and YES to PDYA [Re: ranonar]
#20144280 - 06/17/14 10:38 AM (9 years, 8 months ago) |
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Quote:
ranonar said: You may inoculate with a liquid but do the growing on something solid if you want to keep te contam risk down.

I don't think anyone has been able to grow on liquid yet. RR had a contest a few years back for a microscope for anyone who could grow a 10gram wet fruit on liquid. I do not believe he ever had to pay out
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 4 days, 1 hour
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#20144408 - 06/17/14 11:14 AM (9 years, 8 months ago) |
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he ment growing mycelium not fruiting
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Still does not make sense 
I guess I find most of the terminology fairly sufficient. Liquid culture is liquid culture. Multispore solution is multispore solution. Easy. I personally like LC for a few things. I don't like the idea that its easy or noob friendly. To do it right takes care and sterile tek that you will not learn firing spores into sugar water. But I still have some fun with it
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nucleophile
international cowboy chemist


Registered: 04/28/13
Posts: 299
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#20144547 - 06/17/14 11:47 AM (9 years, 8 months ago) |
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I fruited a mushroom in an LC once. I'd call it inefficient at best, hehe.
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Dos Ounce
Fighter of the Nightman



Registered: 09/07/12
Posts: 873
Loc:
Last seen: 3 years, 7 months
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Re: Just say NO to LC and YES to PDYA [Re: PussyFart]
#20144613 - 06/17/14 12:04 PM (9 years, 8 months ago) |
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Quote:
PussyFart said:
Quote:
MonkeyJesusFresco said: L.I. is a new term for me, but I imagine it's like a "grain"-LC. where you take your clean, fully colonized grain jar, add sterile water, shake withdraw the liquid (that now has little bits of mycelium floating in it) and...TA-DA! a liquid inoculation.
The benefit there being, that there's not an excess of nutrients for contamination to run rampant on..
If there is enough nutrients for the LC to grow afterwards, there is enough for contams.
I don't think there is :/ maybe I misinterpreted but I think it is basically just to hold the mycelia solution until you use it. Don't think the mycelia actually "grows" in it.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Just say NO to LC and YES to PDYA [Re: Dos Ounce]
#20144630 - 06/17/14 12:08 PM (9 years, 8 months ago) |
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Trich is predatory and will feed on the mushroom myc. In that case there would always be nutes for it.
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Dos Ounce
Fighter of the Nightman



Registered: 09/07/12
Posts: 873
Loc:
Last seen: 3 years, 7 months
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#20144636 - 06/17/14 12:08 PM (9 years, 8 months ago) |
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ahh, that makes sense
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Djchrist
Student



Registered: 06/06/11
Posts: 618
Loc: South Africa
Last seen: 6 months, 16 days
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Re: Just say NO to LC and YES to PDYA [Re: Dos Ounce]
#20148529 - 06/18/14 05:59 AM (9 years, 8 months ago) |
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 Bookmarked
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AIRDOG



Registered: 10/16/99
Posts: 3,493
Loc: world's shroom capital
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#20537749 - 09/08/14 01:06 PM (9 years, 5 months ago) |
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can I use bakers yeast? its the only one I have
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blueconfusion
Strangest


Registered: 12/14/12
Posts: 1,727
Last seen: 15 hours, 46 minutes
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Re: Just say NO to LC and YES to PDYA [Re: AIRDOG]
#20537821 - 09/08/14 01:24 PM (9 years, 5 months ago) |
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Don't use bakers yeast it's not the same thing just forget the yeast it's not necessary
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#20537946 - 09/08/14 01:47 PM (9 years, 5 months ago) |
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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blueconfusion
Strangest


Registered: 12/14/12
Posts: 1,727
Last seen: 15 hours, 46 minutes
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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
#20539246 - 09/08/14 05:59 PM (9 years, 5 months ago) |
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Read a bunch of old posts on here I wonder if loki ever did his glc
Edited by blueconfusion (09/09/14 02:53 AM)
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 4 hours, 48 minutes
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#20539992 - 09/08/14 07:54 PM (9 years, 5 months ago) |
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Who cares
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blueconfusion
Strangest


Registered: 12/14/12
Posts: 1,727
Last seen: 15 hours, 46 minutes
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Re: Just say NO to LC and YES to PDYA [Re: Stromrider]
#20541702 - 09/09/14 05:57 AM (9 years, 5 months ago) |
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Quote:
Stromrider said: Who cares 

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