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Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 3 months
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion] 1
#19276259 - 12/14/13 07:34 PM (10 years, 2 months ago) |
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Quote:
blueconfusion said: mea is really easy too!!! you ever try putting yeast in that i've read that works really well.
i dont have yeast atm, but gonna give it a whirl
-------------------- SPREAD THE SPORES
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 4 hours, 48 minutes
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Re: Just say NO to LC and YES to PDYA [Re: blueconfusion]
#19276264 - 12/14/13 07:35 PM (10 years, 2 months ago) |
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Quote:
blueconfusion said: got plenty here something like 6-12"!
You suck
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Just say NO to LC and YES to PDYA [Re: Stromrider]
#19276289 - 12/14/13 07:42 PM (10 years, 2 months ago) |
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I'm not even going to start on about the 24"+ that I am cursing right fucking now. Plus its cold as fuck, got down to -20f last week. Fucking bullshit. Barely can keep my grow area above 69F, everything is going slow as fuck.
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sharpshroomer98
Student of the Universe.



Registered: 08/30/11
Posts: 252
Last seen: 10 hours, 8 minutes
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19276302 - 12/14/13 07:44 PM (10 years, 2 months ago) |
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Damn I thought here with the greatest snow on earth we had it bad lol. We've been under 10 the last week or so. Damn cold.
-------------------- No, I wasn’t born in 98’
 
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 4 hours, 48 minutes
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Man screw all yyou guys! I never get snow!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Just say NO to LC and YES to PDYA [Re: Stromrider]
#19276329 - 12/14/13 07:48 PM (10 years, 2 months ago) |
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i'm up in bc and rarely get it, and when it does come it goes as fast as it showed up
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
#19276342 - 12/14/13 07:51 PM (10 years, 2 months ago) |
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It could be worse. A few years back it got down to -53.5F. I was tempted to build an incubator at that point 
Quote:
cronicr said: i'm up in bc and rarely get it, and when it does come it goes as fast as it showed up
Screw you Van island pussies
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 4 hours, 48 minutes
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19276349 - 12/14/13 07:53 PM (10 years, 2 months ago) |
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Uh oh its about to get ugly in here. Well as ugly as it can get by Canadians
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19276355 - 12/14/13 07:54 PM (10 years, 2 months ago) |
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Quote:
Screw you Van island pussies 
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Just say NO to LC and YES to PDYA [Re: cronicr]
#19276361 - 12/14/13 07:55 PM (10 years, 2 months ago) |
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and i'm still waiting on that contam timelapse....just give your vag a wipe n swipe it to agar for me baby!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Just say NO to LC and YES to PDYA [Re: Stromrider]
#19276371 - 12/14/13 07:57 PM (10 years, 2 months ago) |
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Quote:
Stromrider said: Uh oh its about to get ugly in here. Well as ugly as it can get by Canadians 
Shit, when you live in Oilberta it can get damn ugly. I got lost driving through L.A. once and found out I was in the Ghetto. I'm like "damn" this is nicer than my hood Take a drive through the reservations round here and ya see bullet riddled pig cruisers on fire on the corners
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Just say NO to LC and YES to PDYA [Re: Pastywhyte]
#19276386 - 12/14/13 08:01 PM (10 years, 2 months ago) |
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Quote:
Pastywhyte said: Take a drive through the reservations round here and ya see bullet riddled pig cruisers on fire on the corners 
hell yeah and AMEN!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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ranonar
Stranger
Registered: 07/27/06
Posts: 65
Last seen: 9 years, 8 months
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Re: Just say NO to LC and YES to PDYA [Re: TiN 42]
#20138219 - 06/16/14 06:15 AM (9 years, 8 months ago) |
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A few small adaptations to this recipe:
I would rather use potato powder (potato starch) than fresh potatoes. I rather use marmite (= yeast&vegetable extract) than just yeast I do not pour the liquid in petris but transfer it from the bottle to the petris with a large (20ml) syringe.
4.0 grams potato starch 20.0 grams dextrose 15.0 grams agar 2 grams of Marmite
I have dissolved one small jar of Marmite in 250ml water. Filled a bottle with it and sterilized that one. Now add 4-5ml of this in 1 liter of PDA or other agar medium.
I use a syringe for liquid agar transfer because pouring agar often causes spills, and then those become a vector for contamination.
About the (G)LC versus agar discussion: I think it is a bit more complicated since it mattters if you are inoculating a master or an end substrate. I am on the side of RR and others in that a 2 dimensional (agar) surface -which is itself inoculated by a pinch of spores, or a wedge of agar or a kernel of spawn- is the most secure way to avoid contaminations. But it is perfectly fine to use an agar plate to make a liquid inoculant for immediate use.
I hesitate to recommend a liquid inoculant to be used on any substrate which needs to be shaken. After the shake there is no way to find out the origin of a contamination. Liquid inoculants are fine for a BRF/verm (PF) substrate but injecting it in bags or jars of whole grain or rye grass substrates adds some risk. Which is managable as long as those bags or jars are not to be used as grain masters (to inoculate more grain jars).
IMHO future Grain Masters need to be inoculated by agar wedge. I think that is the central dogma.
Liquid inoculants are fine for end substrates such as PF jars and grains which are to be cased and fruited. Be it that liquid inoculants are risky for substrates which are to be shaken.
To make it even more complicated, in my observation LCs can have a better shelf life than spore prints. At least up to 10 years at room temp. But these long term storage LCs are not easy to prepare. They need to be stored in a special way. And need to be tested a month or so after they are made and then once a year. Invisible contamination is a huge risk with any stored LC.
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 4 days, 1 hour
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Re: Just say NO to LC and YES to PDYA [Re: ranonar]
#20138370 - 06/16/14 07:40 AM (9 years, 8 months ago) |
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oh...so the "just say no" campaign...that a thing huh?
-------------------- LAGM v 2.024 - endo cabendo
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ranonar
Stranger
Registered: 07/27/06
Posts: 65
Last seen: 9 years, 8 months
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I do not know if it is a real campaign. But it is a nice banner I wonder in which thread it first appeared and why exactly. But I am following the (sometimes heated) discussions about LC closely. They are interesting!
But the discussions are often not detailed enough. There is a difference between LC and LI (liquid inoculant). An LI can be drawn from an LC or freshly made from a colonized solid substrate. The latter is often a good idea.
All over the internet LC's are promoted as a good tek to stretch a syringe. And -IMHO- that is often not a good idea because of the danger for hidden contaminants. for that reason I think it is a good idea to get a warning out. The 'campaign' is a good idea. If you want a real safe way to stretch a syringe, then inoculate a BRF/verm jar, use a small block of it to put on an agar plate, wait until that is colonized and make a LI from that.
But subculturing agar plates over and over again is also a fast route to degeneration. You need something for long term storage, and spores are not always an option. Blocks of colonized agar in sterile water is the best known alternative but it is not super convenient (a SAB or other sterile environment is required to handle those). So if you really know what you are doing and know how to handle the risks then LCs can indeed be a viable option to save a culture for a decade or more at roomtemperature.
For me - I try to avoid LCs. But I use LIs quite often. And sometimes I depend on LCs for long time storage. When I look at it that way the 'campaign' goes too far. But perhaps I am taking it all to serious.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Just say NO to LC and YES to PDYA [Re: ranonar]
#20139847 - 06/16/14 02:06 PM (9 years, 8 months ago) |
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Quote:
ranonar said: But the discussions are often not detailed enough. There is a difference between LC and LI (liquid inoculant). An LI can be drawn from an LC or freshly made from a colonized solid substrate. The latter is often a good idea.
So wait, LC can't be drawn from an LC or freshly made from a colonized solid substrate?
Why would one be better than the other?
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 4 days, 1 hour
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Re: Just say NO to LC and YES to PDYA [Re: SpitballJedi]
#20140573 - 06/16/14 04:50 PM (9 years, 8 months ago) |
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Get this:
to prepare a LC, you whip up some water and nutrients add spores/mycelium and...TA-DA! LC.
L.I. is a new term for me, but I imagine it's like a "grain"-LC. where you take your clean, fully colonized grain jar, add sterile water, shake withdraw the liquid (that now has little bits of mycelium floating in it) and...TA-DA! a liquid inoculation.
The benefit there being, that there's not an excess of nutrients for contamination to run rampant on..
just my 2cents though
-------------------- LAGM v 2.024 - endo cabendo
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Quote:
MonkeyJesusFresco said: L.I. is a new term for me, but I imagine it's like a "grain"-LC. where you take your clean, fully colonized grain jar, add sterile water, shake withdraw the liquid (that now has little bits of mycelium floating in it) and...TA-DA! a liquid inoculation.
The benefit there being, that there's not an excess of nutrients for contamination to run rampant on..
If there is enough nutrients for the LC to grow afterwards, there is enough for contams.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 4 days, 1 hour
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Re: Just say NO to LC and YES to PDYA [Re: PussyFart]
#20140626 - 06/16/14 05:00 PM (9 years, 8 months ago) |
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I've actually dreamed up a few contraptions/techniques for filtering out and "washing" the mycelial growth of a LC, washing away excess nutrients and whatever the word for "fungus-poop" is; leaving a sterile mycellium suspension...it's called the "Fuck that!"-tek
-------------------- LAGM v 2.024 - endo cabendo
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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