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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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SHIPs vs Opening Lid Thread 4
#20116427 - 06/11/14 08:55 PM (9 years, 8 months ago) |
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For those who may not know, SHIP stands for self-healing injection port and is usually made by putting a hole in your jar lid and filling the whole with RTV. Then, you stick your needle through the RTV to inoculate. This is done to avoid opening the jar for inoculation.
I was just sitting here wondering if SHIPs are more of a noob thing or if long time growers still use them.
I used to use SHIPs because I thought it would help with my contamination issues, but it didn't.
After a while, I realized I have to open the jar anyway to G2G (grain to grain transfer).
Then once I got in to agar, I think my fate was sealed, SHIPs became obsolete for me.
Even when I go back to a MS syringe, I find opening the jar to be quite simple and low risk in a SAB.
Do you still use a SHIP? Why or why not? How long have you been growing? Is your experience similar to mine?
In my humble opinion, I think you might as well get used to opening jars in a SAB if you plan on cultivating a while. Ditch the SHIP.
Disagreement and debate is not the same as flaming.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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Hyphai
Registered: 04/03/14
Posts: 517
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I do a little bit of everything. I mostly use SHIP's when working with liquid culture, or have a spore syringe of a species I am just start working on. Besides that Im using prints and agar and opening the lids to do my work.
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Mdahmer
Aloysius devadander abercrombie



Registered: 04/05/14
Posts: 2,516
Last seen: 6 years, 7 months
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My first 5 qt jars I made SHIP's and was very proud of how they looked and worked..then I got into agar and realized I will probably never use a syringe again except maybe with an LC for these atl7s I'm about to do soon so if the jars are open at the time I'll use them but all my qts now just have a GE hole...I'm pretty new to this but I just don't see them really being all that useful so far
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OH1Ogrown
Student



Registered: 04/23/12
Posts: 1,214
Loc: Treasure Coast
Last seen: 10 months, 3 days
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Re: SHIPs vs Opening Lid Thread [Re: Mdahmer]
#20116524 - 06/11/14 09:16 PM (9 years, 8 months ago) |
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I recently discontinued my SHIP lid making. I did a spore solution inoculation with my new plastic lids without SHIPs. Honestly I probably won't go back. RTV is a waste of time and money. If somebody doesn't want to build an SAB and get serious, then let them play with stinky red gunk. I'm popping lids all day. Screw, squirt, screw, done. My sterile needle touches nothing in or out.
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solomilitia
The offender

Registered: 03/25/14
Posts: 1,419
Last seen: 9 years, 8 months
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Re: SHIPs vs Opening Lid Thread [Re: OH1Ogrown]
#20116577 - 06/11/14 09:27 PM (9 years, 8 months ago) |
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I'm still using SHIPs I found I have a nak for making clean LC and just have not moved on But a shroomery guy sent me a print today and I will be forced in to agar to work with the print I also use a SAB do you guys find that open jars in the SAB are imune to contams ?
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NEVER AGAIN UNPUNISHED http://www.wolf-pac.com/
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: SHIPs vs Opening Lid Thread [Re: solomilitia]
#20116596 - 06/11/14 09:32 PM (9 years, 8 months ago) |
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no technique is completely immune to contamination, but a SAB is pretty standard and extremely effective.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,306
Loc: South East USA
Last seen: 9 minutes, 6 seconds
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Re: SHIPs vs Opening Lid Thread [Re: OH1Ogrown]
#20116605 - 06/11/14 09:33 PM (9 years, 8 months ago) |
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on occasion I'll make a few SHIPs..."just in case", I don't necessarily use 'em
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,819
Loc: Canada
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Half my lids have SHIP's which is funny cause I have yet to use them. I went from pf cakes to agar and made a bunch of lids following the teks exactly, and then opened the lids to inoculate with wedges As it is I have yet to actually pull off a monotub that used grains inoculated with ms solution. The few attempts I have made usually didn't make it and served as a reminder of why I love agar
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airdragon333
LC Advocate lol



Registered: 03/14/14
Posts: 325
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Re: SHIPs vs Opening Lid Thread [Re: OH1Ogrown]
#20116627 - 06/11/14 09:40 PM (9 years, 8 months ago) |
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 I wouldn't feel nearly as secure about my LC's if I didn't do it right. There's usually some oddball project or idea I have where these things come in useful as well.
Edit: Shameless misdirection to get help here.
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I'm not a mycologist but I did stay at a Holiday Inn last night.
Edited by airdragon333 (06/11/14 09:57 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,819
Loc: Canada
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Quote:
airdragon333 said:
 I wouldn't feel nearly as secure about my LC's if I didn't do it right. There's usually some oddball project or idea I have where these things come in useful as well.
All my LC lids are also proper SHIP and Whatman filters, but I never use those for grains
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airdragon333
LC Advocate lol



Registered: 03/14/14
Posts: 325
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Re: SHIPs vs Opening Lid Thread [Re: Pastywhyte]
#20116745 - 06/11/14 10:02 PM (9 years, 8 months ago) |
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Quote:
Pastywhyte said: I never use those for grains 
I'm with you there, I prefer a more ghetto approach to grains. I don't even wanna mention postal tyvek for fear of response -oops *Cough, look over here cough*
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I'm not a mycologist but I did stay at a Holiday Inn last night.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: SHIPs vs Opening Lid Thread [Re: Pastywhyte]
#20116748 - 06/11/14 10:03 PM (9 years, 8 months ago) |
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i never used a ship, home made or not, on grain jars. i did make a home made lc one, but now i use the "what man" vendor style lid for LC's cause they were given to me by a friend (but they are really nice to work with). cracking the lid to drop spores or lc is the exact same, if not safer, than opening the lid ALL THE WAY, and KEEPING IT OPEN FOR LONGER with a g2g transfer in my opinion. we should all be using an SAB or hood anyway, so that should go without saying, in case someone bring up the "i use ship cause i don't have SAB" argument.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Quote:
I don't even wanna mention postal tyvek for fear of response -oops *Cough, look over here cough*
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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airdragon333
LC Advocate lol



Registered: 03/14/14
Posts: 325
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I'm not a mycologist but I did stay at a Holiday Inn last night.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,819
Loc: Canada
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: SHIPs vs Opening Lid Thread [Re: Pastywhyte] 1
#20260316 - 07/11/14 10:11 PM (9 years, 7 months ago) |
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Quote:
I was just sitting here wondering if SHIPs are more of a noob thing or if long time growers still use them.
Just a simple question. Which scenario would be more prone to contamination:
Scenario 1: Grain jar in a SAB, lid cracked open and grains inoculated with 1 cc of MS solution.
Scenario 2: Grain jar in a SAB, grains inoculated through a SHIP with 1 cc of MS solution.
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Honestly I would say the ship method simply because your needle has to touch something. Guess it depends on how dirty your sab is and how still your air is.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Quote:
TheEaglesGift said:
Quote:
I was just sitting here wondering if SHIPs are more of a noob thing or if long time growers still use them.
Just a simple question. Which scenario would be more prone to contamination:
Scenario 1: Grain jar in a SAB, lid cracked open and grains inoculated with 1 cc of MS solution.
Scenario 2: Grain jar in a SAB, grains inoculated through a SHIP with 1 cc of MS solution.
the ship, i personnely line my jars up left to right and make sure the lids are easily cracked and wiped down, heat my syringe up and and start cracking lids and shooting solution in, a few jars in one shot and the needle touches nuthing
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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nucleophile
international cowboy chemist


Registered: 04/28/13
Posts: 299
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Re: SHIPs vs Opening Lid Thread [Re: cronicr] 1
#20260555 - 07/11/14 11:05 PM (9 years, 7 months ago) |
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A hypodermic needle works for injections because it's very, very smooth and sharp so it won't pick up contaminants off a surface. I'd think that for the first injection, the needle would have pretty much zero risk of carrying contaminants, but after a few uses it does dull (take a close look at the tip of a needle that's been used, it ceases to be smooth quite quickly) which would increase the risk of picking up any lingering contaminants on the port.
I'd say for a fresh needle, the risk would be about equal if you had good sterile technique with both procedures, but if you're reusing syringes I'd bet the older needles would have more risk of contamination whereas a cracked lid wouldn't be any riskier than agar plates, so if you don't usually see satellite molds on your agar you should have excellent success cracking the lids.
I think maybe the worn needle is one of the reasons LCs mysteriously contaminate sometimes as well although I can't state it for an absolute certainty.
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OH1Ogrown
Student



Registered: 04/23/12
Posts: 1,214
Loc: Treasure Coast
Last seen: 10 months, 3 days
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Re: SHIPs vs Opening Lid Thread [Re: nucleophile]
#20260784 - 07/12/14 12:10 AM (9 years, 7 months ago) |
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Since this is back up on my list, I'd like to update that I no longer utilize needles or SHIPs and my contam rates have dropped tremendously.
I find it so much easier just to pop tops and inoculate these days.
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