|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
lateforthafuture
fanatic


Registered: 03/26/07
Posts: 471
Last seen: 4 years, 3 months
|
Clone > Agar> Grain > G2G Pics *Udpated* 1
#20099470 - 06/08/14 08:07 AM (10 years, 7 months ago) |
|
|
So I cloned this cluster from a MS grow a while ago

It was placed on agar and after a few transfers I used this plate to inoculate some masters


I didn't take any pics of the masters colonizing but I used one master and G2G 10 jars, here they are 5 days after the G2G transfer.





I'm thinking about doing a side by side comparison of CVG VS Straw/CVG using this clone and see what happens. I'll post here with updates, thanks for checking me out 
Little update
The jars finished up colonizing over the weekend so I spawned them Monday and figured I share with you guys.
Here's the jars at 100%


They were spawned to a bulk sub of coir/verm/straw
6 dry qts of straw was soaked in warm soapy water for a few hours then rinsed through a colander with a few gallons of clean (no soap) water. After the rinse I allowed to drain for about 30 mins then squeezed out excess water before adding to the rest of the substrate.

The straw was added to 2 bricks of coir that was hydrated with 8 quarts of water and 4 quarts of vermiculite (I'm doing to monotubs). I usually add a hand full of gypsum per brick of coir but it slipped my mind, shame on me


The substrate was mixed then loaded in turkey bags, zipped tied, and put in a large stock pot on a propane stove to pasteurize. One tubs worth of substrate in each bag.


After the substrate was cooled they were spawned to monotubs using four quarts Colonized WBS. Here they are 48 hrs after spawning.




This is an untested clone so I'm excited to see how it turns out. Hopefully a good fruiting culture.
Update-
Here's the tubs 4 days after spawning



And here we are 6 days after spawning, getting really close to 100% probably start fruiting in the next 24-48 hrs.



-------------------- "Not what could have been, or what should have been, but what will be" -Self
 
Edited by lateforthafuture (06/22/14 06:02 PM)
|
TheApprentice
back at it



Registered: 09/25/11
Posts: 4,727
Last seen: 3 years, 1 month
|
|
Very nice
--------------------
RR Videos -Best $9 Ever Spent *
No Pour AGAR Tek * Easy COIR Trays! *
Pink Oysters on Newspaper TEK
"Yeah? Well, DRACULA called... and he said he's coming over tonight, and I said OK!"
|
tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 21 hours, 19 minutes
|
|
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
|
Re: Clone > Agar> Grain > G2G Pics [Re: tripdawg420]
#20099763 - 06/08/14 09:39 AM (10 years, 7 months ago) |
|
|
Nice hood
|
lateforthafuture
fanatic


Registered: 03/26/07
Posts: 471
Last seen: 4 years, 3 months
|
Re: Clone > Agar> Grain > G2G Pics [Re: Pastywhyte]
#20099944 - 06/08/14 10:37 AM (10 years, 7 months ago) |
|
|
Quote:
Pastywhyte said: Nice hood 
the metal "flashing" or whatever it is called in the front of the filter is banged up pretty good so I actually did all that in my largest SAB, I just don't trust the hood until I buy a new filter, it also needs a pre-filter. But yeah thanks it is nice having a flowhood.
-------------------- "Not what could have been, or what should have been, but what will be" -Self
 
|
Killaloting
Stranger

Registered: 06/03/14
Posts: 98
Last seen: 4 years, 9 months
|
|
Are thoose mckennai? good work
|
spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
|
Re: Clone > Agar> Grain > G2G Pics [Re: Killaloting]
#20099979 - 06/08/14 10:49 AM (10 years, 7 months ago) |
|
|
|
lateforthafuture
fanatic


Registered: 03/26/07
Posts: 471
Last seen: 4 years, 3 months
|
Re: Clone > Agar> Grain > G2G Pics [Re: Killaloting]
#20100001 - 06/08/14 10:53 AM (10 years, 7 months ago) |
|
|
No they're Burma, I pretty sure that the substrate they came from was overly hydrated (a little beyond field capacity) and that's why they have that gnarly look to them. I hope it's genetic but we'll see. It was actually the largest one in the cluster that I cloned.
-------------------- "Not what could have been, or what should have been, but what will be" -Self
 
|
Killaloting
Stranger

Registered: 06/03/14
Posts: 98
Last seen: 4 years, 9 months
|
|
Will definetly follow this one
Edited by Killaloting (06/08/14 12:41 PM)
|
lateforthafuture
fanatic


Registered: 03/26/07
Posts: 471
Last seen: 4 years, 3 months
|
Re: Clone > Agar> Grain > G2G Pics [Re: Killaloting]
#20150538 - 06/18/14 04:42 PM (10 years, 6 months ago) |
|
|
Bump, Update in the first post
-------------------- "Not what could have been, or what should have been, but what will be" -Self
 
Edited by lateforthafuture (06/18/14 04:43 PM)
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
|
|
Looking good so far
|
lateforthafuture
fanatic


Registered: 03/26/07
Posts: 471
Last seen: 4 years, 3 months
|
Re: Clone > Agar> Grain > G2G Pics [Re: Pastywhyte]
#20150683 - 06/18/14 05:17 PM (10 years, 6 months ago) |
|
|
Quote:
Pastywhyte said: Looking good so far 
Thanks pasty!!! I've got a bunch of clones I need to grow out that are on agar right now. Some are actually on grains already but most are clean and on plates, just need to get them on grains. To many projects at once
-------------------- "Not what could have been, or what should have been, but what will be" -Self
 
|
Djchrist
Student



Registered: 06/06/11
Posts: 618
Loc: South Africa
Last seen: 1 year, 5 months
|
|
Keeping an eye on this one
|
lateforthafuture
fanatic


Registered: 03/26/07
Posts: 471
Last seen: 4 years, 3 months
|
Re: Clone > Agar> Grain > G2G Pics [Re: Djchrist]
#20169564 - 06/22/14 06:04 PM (10 years, 6 months ago) |
|
|
Update in the First post
-------------------- "Not what could have been, or what should have been, but what will be" -Self
 
|
lateforthafuture
fanatic


Registered: 03/26/07
Posts: 471
Last seen: 4 years, 3 months
|
|
Here's a little update the tubs have been in fruiting conditions for 3 days now and they're both starting to knot up. It's hard to tell in the pics but hopefully in a few days I'll have a decent pinset with some pin porn for you guys.



-------------------- "Not what could have been, or what should have been, but what will be" -Self
 
|
dietrich
Zauberpilz-Esser



Registered: 07/06/10
Posts: 319
Last seen: 6 years, 7 months
|
|
Very nice, curious to see how they turn out for you!
-------------------- Ich heiße Dietrich der Zauberpilz-Esser und bringe ich Frieden und Liebe für alle.
|
sprinkles
otd president


Registered: 10/13/12
Posts: 21,541
Loc: washington state
Last seen: 5 months, 29 days
|
|
that is freakin amazing!
|
cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
Last seen: 1 minute, 26 seconds
|
Re: Clone > Agar> Grain > G2G Pics [Re: sprinkles]
#20190928 - 06/26/14 07:47 PM (10 years, 6 months ago) |
|
|
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
|
lateforthafuture
fanatic


Registered: 03/26/07
Posts: 471
Last seen: 4 years, 3 months
|
Re: Clone > Agar> Grain > G2G Pics [Re: cronicr]
#20202091 - 06/29/14 06:59 AM (10 years, 6 months ago) |
|
|
Another update, came home from a weekend camping trip and had a look at these tubs, they are both knotting up like crazy but I found a small spot of suspect growth on each tub. The growth in question is right under one of the bottom holes on each tub. Only one spot on each tub though. The first two pics are of the entire tub, the last two are of the suspect growth.
 

In the next picLook at the growth to the right of the polyfill

In this pics there's similar growth right under the polyfill just to the right a little.

Let me know what you guys think. I was thinking it could be some bacteria, possibly some dieing myc. Hopefully not trich (FUCK) 
The thing is my spawn, I'm almost positive it was 100% clean. My pasteurization temp went a little high for a few mins (180 for like 5 mins) and with straw in my bulk sub that makes me nervous. Any in put is appreciated. Thanks
-------------------- "Not what could have been, or what should have been, but what will be" -Self
 
|
TheApprentice
back at it



Registered: 09/25/11
Posts: 4,727
Last seen: 3 years, 1 month
|
|
I can't wait to see your tub!!
--------------------
RR Videos -Best $9 Ever Spent *
No Pour AGAR Tek * Easy COIR Trays! *
Pink Oysters on Newspaper TEK
"Yeah? Well, DRACULA called... and he said he's coming over tonight, and I said OK!"
|
|