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spore baby



Registered: 07/30/13
Posts: 4,918
Last seen: 8 years, 8 months
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Re: LC ozone and time. [Re: Stargate]
#20061176 - 05/30/14 01:52 PM (9 years, 8 months ago) |
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Edited by spore baby (12/20/14 08:32 AM)
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airdragon333
LC Advocate lol



Registered: 03/14/14
Posts: 325
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Yep, if you get into agar you can test your ozone fueled clean box by leaving a plate open for a while, see if anything pops up.
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I'm not a mycologist but I did stay at a Holiday Inn last night.
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Stargate
Addicted To Growing


Registered: 05/26/14
Posts: 1,124
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Quote:
spore baby said: I have a small ozone generator but use it rarely.
Do know that ozone will kill everything over time.
Consider it far worse and stronger than hydrogen peroxide on life forms, including yourself and mushrooms spores,etc.
Don't use it all the time.
Yep, I know that. You have a good point too. Perhaps I should drill a hole in my glove box, and install a one way air escape. I could keep the spores safe inside syringes. After putting everything in the box, I could blast it with ozone for 10 minutes, than wait an hour for it to break up into O2. Thats when its safe in there. Once the O3 has all broken down, than I could start the process. The one way air valve would be due to the O3 expanding upon breaking down. It would let the positive pressure out.
-------------------- From before I started growing gourmet mushrooms:
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: LC ozone and time. [Re: Stargate]
#20063567 - 05/31/14 12:51 AM (9 years, 8 months ago) |
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Quote:
Stargate said:
Quote:
spore baby said: I have a small ozone generator but use it rarely.
Do know that ozone will kill everything over time.
Consider it far worse and stronger than hydrogen peroxide on life forms, including yourself and mushrooms spores,etc.
Don't use it all the time.
Yep, I know that. You have a good point too. Perhaps I should drill a hole in my glove box, and install a one way air escape. I could keep the spores safe inside syringes. After putting everything in the box, I could blast it with ozone for 10 minutes, than wait an hour for it to break up into O2. Thats when its safe in there. Once the O3 has all broken down, than I could start the process. The one way air valve would be due to the O3 expanding upon breaking down. It would let the positive pressure out.
or u could wipe ur jars down with iso, and go from there??? i don't get the fuss about the ozone thing? if u saw the kitchen where i have my SAB in u would b appalled. better safe than sorry is one thing, but going a crazy extra mile when u can probably get away without it seems overkill.. why not just try regular SAB set up, and see how it does? i swipe wild prints in my SAB, and then right after do plate to plate transfers of clean cultures. if u are careful, u can pull a lot off in an SAB. it just takes practice and a passion for the hobby.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Stargate
Addicted To Growing


Registered: 05/26/14
Posts: 1,124
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Quote:
blindingleaf said: or u could wipe ur jars down with iso, and go from there??? i don't get the fuss about the ozone thing? if u saw the kitchen where i have my SAB in u would b appalled. better safe than sorry is one thing, but going a crazy extra mile when u can probably get away without it seems overkill.. why not just try regular SAB set up, and see how it does? i swipe wild prints in my SAB, and then right after do plate to plate transfers of clean cultures. if u are careful, u can pull a lot off in an SAB. it just takes practice and a passion for the hobby.
This is more than just a hobby for me. I'm creating an exotic mushroom business for local high end restaurants. As I am looking at this as a business, I want to be able to have the highest success rates I can produce. As for ozone, why don't you look into it? Its pretty cool stuff.
-------------------- From before I started growing gourmet mushrooms:
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 17 hours, 1 minute
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Re: LC ozone and time. [Re: Stargate]
#20066563 - 05/31/14 08:20 PM (9 years, 8 months ago) |
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i have a high success rate in a sab
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airdragon333
LC Advocate lol



Registered: 03/14/14
Posts: 325
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Re: LC ozone and time. [Re: Stargate]
#20066596 - 05/31/14 08:26 PM (9 years, 8 months ago) |
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Quote:
Stargate said: This is more than just a hobby for me. I'm creating an exotic mushroom business for local high end restaurants. As I am looking at this as a business, I want to be able to have the highest success rates I can produce. As for ozone, why don't you look into it? Its pretty cool stuff. 
If this is going to be a business then you probably want to get into agar. At that point you can use it to gauge the effectiveness of your ozone box and share the results with us. I am very interested in learning the results.
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I'm not a mycologist but I did stay at a Holiday Inn last night.
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Stargate
Addicted To Growing


Registered: 05/26/14
Posts: 1,124
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Quote:
airdragon333 said: If this is going to be a business then you probably want to get into agar. At that point you can use it to gauge the effectiveness of your ozone box and share the results with us. I am very interested in learning the results.
Sounds good to me  And yep, I plan on sharing any findings with you guys. I have clip boards where I will be keeping logs of any results, as well as taking pictures. Not sharing with you guys, when you have taught me so much, would be inconsiderate. 
By the way, what do you guys think of making LC jars by taking a colonized jar, suspending the mycelium in water, than using that to make the LC jars? That way, you can check for contams in the colonized jar in order to have a higher success of no contams in the LCs? Someone here has got to have tried that before. I doubt I can think of anything new this early into getting into this subject.
-------------------- From before I started growing gourmet mushrooms:
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airdragon333
LC Advocate lol



Registered: 03/14/14
Posts: 325
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Re: LC ozone and time. [Re: Stargate]
#20066680 - 05/31/14 08:48 PM (9 years, 8 months ago) |
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I'm not a mycologist but I did stay at a Holiday Inn last night.
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Stargate
Addicted To Growing


Registered: 05/26/14
Posts: 1,124
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Quote:
airdragon333 said: Here's one.;)
Awesome, so it does work!  Thanks for the link. Bookmarking that one 
Has anyone looking at this thread tried it yet? I would love your personal opinion.
-------------------- From before I started growing gourmet mushrooms:
Edited by Stargate (05/31/14 09:33 PM)
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airdragon333
LC Advocate lol



Registered: 03/14/14
Posts: 325
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Re: LC ozone and time. [Re: Stargate]
#20066844 - 05/31/14 09:41 PM (9 years, 8 months ago) |
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Sorry, not I. I do mine the old fashioned (and much frowned upon) way.
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I'm not a mycologist but I did stay at a Holiday Inn last night.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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yes it works. def get into agar tho dude. agar>lc is safer than GLC. also u will need agar to back up ur cultures for storage. thats cool u trying to start a business man, i hope it succeeds! have u looked into flow hoods tho?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 17 hours, 1 minute
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grain jar that was started with agar is safer to make a glc than sugar lc made with agar
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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either way, agar FTW
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 17 hours, 1 minute
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Quote:
blindingleaf said: either way, agar FTW 
fuck ya
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Stargate
Addicted To Growing


Registered: 05/26/14
Posts: 1,124
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Hmmm, alright. Is it best to store the agar cultures in a freezer? I can drag one of my freezers from my other offices over into the shroomery. They go to -30 C. Is that too cold?
For flow hoods, I'll look into making one. Do you see flow hoods being better than SABs?
-------------------- From before I started growing gourmet mushrooms:
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: LC ozone and time. [Re: Stargate]
#20068800 - 06/01/14 11:18 AM (9 years, 8 months ago) |
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flow hood are better than SAB's because u have a lot more freedom of movement. i don't know about storing in freezer, common practice is just in the fridge. but for best results, u want a fridge dedicated to that, with only sterile cultures. my fridge is dirty, and many times a clean plate will get contaminated if its in there too long. slants are best way to store b/c they keep longer and have a much smaller opening, with a screw on top.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Stargate
Addicted To Growing


Registered: 05/26/14
Posts: 1,124
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Alright, I can adjust the freezer to any temp. I'll put it at 5 C, or something like that.
Please clarify what you mean by slants.
-------------------- From before I started growing gourmet mushrooms:
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