Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   Bridgetown Botanicals CBD Concentrates

Jump to first unread post Pages: 1 | 2 | 3 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Agar * 8
    #20048779 - 05/27/14 08:24 PM (9 years, 7 months ago)

This is part of my series "The Basics"

Don't be afraid to get in to agar. There are a lot of teks out there and most of them are dealing with large quantities. This tek is more for those who want to try it on a small scale.

This is a great way to expand your precious spores. One drop on a dish... 5 or 6 dishes...1 dish for 6-10 jars of grain...1 jar of grain to 7-10 jars.... well, you get the picture. I haven't even mentioned cloning.

This tek suits my personal needs because of the small scale that I grow.

I will need:
PC
Bottles
Agar recipe
Petri dishes
Parafilm
I do my pouring in a SAB

Bottles:


I don't do large amounts of agar anymore. I may only pour 5-6 dishes at a time, so Snapple bottles are the perfect size for me.

Also, these bottles fit nicely in my PC.

I scruff the metal lid with a piece of sandpaper and RTV a SFD to it. There is a 1/4" hole in the lid.

Snapple bottles also fit nicely into wide-mouth quart jars. This prevents the bottles from floating around or tipping over. I just stick 7 jars in the PC and then put in whatever number of bottles I'll be using., usually only 1 or 2.


Another thing I like about this method is that, if I'm pouring a bunch of plates, I can leave multiple bottles in the PC. This keeps them hot while I work.

Recipe:
-5g LME
-5g Telephone Brand Agar powder from asian market (or your favorite agar, I use 4g a lot here lately)
-250ml water

This recipe is 1g each of agar and LME per 50ml of water. You can make it less rich and potential promote rhizomorphic growth by cutting back on the LME to .8g per 50ml of water.

This recipe will fill a bottle a little over half way. You can scale to whatever volume you want.

Next:

~Heat water and pour in to the bottle.

~Put LME and agar in heated water and swirl around until mixed. Some say you don't need to heat and mix first, and you really don't have to. I just like to. I find that putting the hot water in first prevents sticking to the bottom of the bottle.

~Put lid on bottle and cover with foil.

~PC at 15psi for 30mins following standard PC procedures. I fill the PC with water to just about the top of the agar level. This prevents boil over as the PC cools down.

~Let agar cool enough to handle but still be in liquid form. It can be tricky at first, to get your dishes poured before the agar congeals. That's why it's a good idea to have more than you think you will need. The cooler you let your agar get, the less condensation you have on the lid of your petri dishes.

~I open my sleeve of petris in my SAB. Since I only use a few at a time, I go ahead and stick the ones I don't use in to a ziplock bag. I put 4 in a quart bag and and then those bags go in a gallon. A whole 25 dish sleeve fits easily in a gallon this way. I always use new ziplocks.

~When your agar is ready, pour in Petri dishes just enough to cover the bottom. You can put in as much as you want, but the mycelium is only going to be growing on the surface. Anymore than a thin layer is a waste.

~ I put the poured dishes in a new ziplock bag until I'm ready to use them. I don't wrap in parafilm until after inoculated.

You can put a cup of hot water on top of your stack of dishes to keep them from fogging up as they cool.

Well, there you have it. That's how I do agar.

I have about 99.9% success with this method.

Feel free to PM me with questions, tips, or comments.

If you follow my process and still have trouble, feel free to ask me for help.

If something I do is unclear, feel free to ask for clarification.

Don't bother to ask if you can omit or substitute any of my steps or materials. I didn't go through the trouble of development and writing just to tell you to do it different.

Just a reminder, this is just how I do it. I've tried a myriad of different methods and this is what I have come to rest on. I'm sure your way works fine, but I simply just don't care. :shrug:. Feel free to make adjustments as you see fit.

Good luck and happy cultivations.

---Spitball


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Edited by SpitballJedi (05/06/16 11:25 AM)


Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Agar [Re: SpitballJedi] * 1
    #20048990 - 05/27/14 09:23 PM (9 years, 7 months ago)

i can only fit 20 in a gallon zip lock, but i don't pre bag them in groups before, i just shove them in, 4x stacks of 5 dishes.    snapple bottles are the best for pouring, i use two and just do a whole sleeve at once.  great write up!


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 7 days
Trusted Cultivator
Re: Agar [Re: blindingleaf]
    #20049369 - 05/27/14 11:03 PM (9 years, 7 months ago)

:super:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 7 days
Trusted Cultivator
Re: Agar [Re: cronicr]
    #20236799 - 07/07/14 11:44 AM (9 years, 6 months ago)

bumping some good threads today, hows your guys's day going?


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: Agar [Re: cronicr]
    #20236808 - 07/07/14 11:47 AM (9 years, 6 months ago)

Awesome. Had a good weekend and almost finished with lunch. How are you cron?


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 7 days
Trusted Cultivator
Re: Agar [Re: SpitballJedi]
    #20236813 - 07/07/14 11:48 AM (9 years, 6 months ago)

doing great
! getting ready to move finally! start my edibles up again


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: Agar [Re: cronicr]
    #20236818 - 07/07/14 11:50 AM (9 years, 6 months ago)

sweet. I got my eye on you


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Extras: Filter Print Post Top
Offlinecinderblock
Failed Conformist
Registered: 07/13/14
Posts: 866
Last seen: 1 year, 4 months
Re: Agar [Re: SpitballJedi] * 1
    #20491766 - 08/29/14 02:59 AM (9 years, 4 months ago)

In a recent post, you mentioned you don't use the Snapple bottle anymore. What do you use now?


Extras: Filter Print Post Top
InvisibleE.FTS
Male


Registered: 08/28/14
Posts: 467
Re: Agar [Re: cinderblock]
    #20491816 - 08/29/14 03:59 AM (9 years, 4 months ago)

:whathesaid:  Interested as well.


Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: Agar [Re: E.FTS]
    #20492023 - 08/29/14 06:55 AM (9 years, 4 months ago)

I use a 1L Pyrex media bottle. I don't like it much either because it has "after drip".


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,854
Last seen: 11 hours, 25 minutes
Trusted Cultivator
Re: Agar [Re: SpitballJedi]
    #20492683 - 08/29/14 11:00 AM (9 years, 4 months ago)

I use a small soy sauce bottle. No problems at all with pouring.


--------------------


Extras: Filter Print Post Top
InvisibleMushiez
 User Gallery


Registered: 04/28/14
Posts: 1,057
Re: Agar [Re: Kizzle]
    #20492698 - 08/29/14 11:03 AM (9 years, 4 months ago)

Quote:

Kizzle said:
I use a small soy sauce bottle. No problems at all with pouring.




Thanks kizzle, I'll have to try that

:takingnotes:


Extras: Filter Print Post Top
InvisibleMudaFuka
Poppin bottles
Male User Gallery


Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
Re: Agar [Re: Mushiez]
    #20492921 - 08/29/14 12:14 PM (9 years, 4 months ago)

I use a long necked licker bottle. It works great.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


Edited by MudaFuka (08/29/14 01:05 PM)


Extras: Filter Print Post Top
OfflineNos-hroom
Oneironaut's Apprentice
Male


Registered: 08/13/14
Posts: 183
Last seen: 8 years, 7 months
Re: Agar [Re: MudaFuka]
    #20498696 - 08/30/14 05:36 PM (9 years, 4 months ago)

I really like the soy sauce bottle idea. I'm assuming we're talking something like this.



As a cook, I'll tell you that few bottles pour a nicer, cleaner, finer stream. I'll have to give it a go. Still, the whiskey bottle is a favored, simple shape. I think RR even recommended and used one in his DVD's section on agar.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,808
Loc: Canada Flag
Trusted Cultivator
Re: Agar [Re: Nos-hroom]
    #20498785 - 08/30/14 05:54 PM (9 years, 4 months ago)

If your planning to pour through that little spout I would recommend against it. The agar will probably start to cool in the small aperture and clog the spout.


Extras: Filter Print Post Top
OfflineNos-hroom
Oneironaut's Apprentice
Male


Registered: 08/13/14
Posts: 183
Last seen: 8 years, 7 months
Re: Agar [Re: Pastywhyte]
    #20498813 - 08/30/14 05:59 PM (9 years, 4 months ago)

Cheers, that's a good point. Shame, but there are several other options. I have read of success with those diner-style coffee carafes with the pour-spouts controlled by little levers or buttons. I'd undoubtedly like to give that one a shot.


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,854
Last seen: 11 hours, 25 minutes
Trusted Cultivator
Re: Agar [Re: MudaFuka]
    #20499006 - 08/30/14 06:48 PM (9 years, 4 months ago)

Quote:

MudaFuka said:
I use a long necked licker bottle. It works great.




:thumbup:

That's the same reason I use such a small bottle. It's fits in the microwave. The agar has usually resolidified by the time I get around to taking it out of the PC.


--------------------


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 19 days
Trusted Cultivator
Re: Agar [Re: Kizzle]
    #20499303 - 08/30/14 08:05 PM (9 years, 4 months ago)

LazyAss, Jking. I recently tried the pastyplates. While I love them. I miss the good old Extra Virgin Oil Oil Bottle I used. I am building a flow hood this week so I am going back to the plastic petri dish agar. Nothing against the No Pore, I just prefer Petris.


Extras: Filter Print Post Top
Offlinecinderblock
Failed Conformist

Registered: 07/13/14
Posts: 866
Last seen: 1 year, 4 months
Re: Agar [Re: SpitballJedi]
    #20663748 - 10/05/14 09:02 PM (9 years, 3 months ago)



Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Agar [Re: cinderblock]
    #20664105 - 10/05/14 10:11 PM (9 years, 3 months ago)

i dunno where SBJ is...(where u at dude?!?!)

but yes to both :thumbup:

2.5 squares of that 4" roll will be enough to seal 4 plates easily.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | Next >  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   Bridgetown Botanicals CBD Concentrates


Similar ThreadsPosterViewsRepliesLast post
* buying agar - help sinisha 3,416 6 03/31/06 05:49 AM
by FractalXplora
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 7,382 8 02/14/05 07:57 AM
by Roadkill
* Potato Dextrose Agar X Malt Extract Agar Alegalize 6,155 8 02/25/05 11:03 AM
by agar
* test tubes agar fungophiliac 3,134 8 06/19/20 09:41 PM
by gorilliam
* LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! agentstone 4,587 12 03/30/20 10:37 AM
by Surf707
* Agar and the global economy Dem_Bones 1,650 4 03/01/06 09:08 AM
by Dem_Bones
* Agar question WormholeSurfer 1,482 3 10/29/05 07:10 PM
by agar
* Agar's Home Made Compost recipe for bulk substrates
( 1 2 3 4 all )
agar 20,876 75 02/12/21 05:50 AM
by Bart84

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
11,998 topic views. 35 members, 310 guests and 77 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.033 seconds spending 0.011 seconds on 14 queries.