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InvisibleSpitballJedi
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My SAB (Still Air Box) * 31
    #20048771 - 05/27/14 10:21 PM (6 years, 4 months ago)

This is part of my series "The Basics"

Why an SAB?
A very important part of cultivation is a clean work environment. Once you sterilize most things such as agar, BRF/PF Cakes, grains, and others, they must remain sterile until 100% colonized.

But, you need to inoculate this stuff? How do you do it without contaminating your sterile stuff inside?

There are two main trains of thought on this: Make your lids with SHIPs or create a sterile work environment so you can just open the jar without contaminating it.

I'm in the later category, make a sterile environment. If you are interested in SHIPs, use the search feature to find out what they are. I don't use them.

Most people use a SAB because they are cheap and easy to make. Flowhoods are nice, but expensive and take more skill to build.

I'm here to describe my SAB

The most important thing about a SAB is still air. If the air is still, then it can't blow contaminants in to your work.

Having gloves attached means you can't flame sterilize outside the box. It also means your hand movements create strong vacuums that result in dirty air getting sucked in and moving around inside. Wear the gloves instead.

A SAB does not need to be sterile, just clean and still.

Here's my SAB (Still Air Box)



I made it from  a Sterlite 1478 from Wal-Mart.

It's 108quarts. Plenty of room to get things done.



The process is simple:

Don't get too caught up in exact measurements. You mostly want to go by feel. Every SAB will be slightly different based on your body size.

I simply put the tote in to the position I would mostly be using it in. I sat it on my table and sat in my chair. This is where I'll be doing my sterile work.

Then, I put my arms straight down to my side and then just bent at the elbows so my hands were straight out in front of me. I eyeballed were my middle finger lined up with the tote and made that my holes' center. This is the best way to figure out what the most natural place for the holes should go.

The 2 armholes are shoulder width apart. Some people don't realize how important it is to make the center of the holes about shoulder width apart. This gives it a nice comfortable feel.

I cut the holes with a 5" hole-saw on my cordless drill. If you lay the tote down on a piece of wood while you drill and go slow, you can prevent cracking.

Notice the lid is missing? That's because I turn the SAB upside down on a towel. When I spray the inside, the towel gets damp and traps contams. The towel also serves as a gasket to prevent drafts.



The holes where the handles were have been tapped.



Getting Ready For Doing Work

I lay the towel down on the table. I have large bath sized towels, so I fold it over. Then, I spray the towel with soap water. I usually use Dr Bronner's Almond Scented Magic Soap. It's what I shower with, but any soap will do. It doesn't take much, just a couple of drops per liter of water. The soap just keeps the water from evaporating too quickly and gives it a little stickiness. I don't see the point in sanitizing the table because it's going to have a towel over it, but do it if it makes you feel good.

Then, I clean the walls of the SAB with 70% IPA. After that, I give a spritz of soap water on the walls and turn the SAB upside down on the towel. Contaminants will stick to wet surfaces. I find soapy water works best. Bleach irritates my nose and lungs. Alcohol dries fast and is flammable.

You can either have the jars or whatever on the towel and cover with the box or feed everything through the arm-holes; it doesn't matter at all.

Once my stuff is in, I let everything just settle for a few minutes, maybe 2-5 minutes. Then, I wipe all my jars or whatever with 70% IPA.

Tips


    As Already mentioned, the point of a SAB is to have a place of still air to do sterile work. We call it sterile work because you want to keep your sterilized stuff sterile. The inside of the SAB is not sterile, just still. I put the SAB in the least drafty room, close the door, and turn off anything that creates drafts or air circulation.

Let things settle before wiping down jars in your SAB.

    I wear nitrile exam gloves and tyvek sleeves. I actually wear the top half of a tvek suit with the legs cut off. You can just wear a clean long-sleeve shirt though. When not in use, I keep the tyvek in a plastic bags just to keep the dust off. I actually keep a lot of stuff wrapped up in bags or totes to keep the dust off.

    Do all flame sterilizations outside the SAB. Heat from the flame creates enough current inside the SAB to cause contams to float around and get in to your sterile work.

    It's a fact, attaching gloves to your SAB turns it in to a Glove-box. Attaching the gloves causes more air to move around so therefore, by definition, it is no longer a Still Air Box. I'm sure there are all kinds of ways to prevent contaminated air from coming in to your GB, but you better make sure the inside is already sterile or it will be pointless. Good luck getting it sterile.

    Do your work toward the back of the SAB to help prevent contams from getting in your sterile work via the arm holes.

    Work diligently. Don't get in a hurry or work at a snails pace. Make all your movements deliberately. Getting in a hurry can stir up the air inside. Working too slow leaves you open for higher potential of getting contams.

    Crack the lids on jars before you put them in the SAB. A stuck lid is a bitch to open inside it. What I mean is, barely unscrew the metal ring or plastic lid so you don't have to fight it inside the box. If using the 2 piece metal lids, don't try to break the seal on the lid part, just barely loosen the ring. Once they are all wiped down and I'm ready to actually start working, I like to loosen all the lids so that I can finish removing them with one hand.

    You don't have to shower the walls. You just want a little spritz. Too much, especially in front, will make it hard to see what you're doing. If you are doing much work or working with warm agar, you'll notice condensation building up on the walls anyway. There shouldn't be many contams floating around and it doesn't take much to trap them.

    Keep movement over open sterile containers at a bare minimum. I don't like to sit anything dow. This takes a little practice, planning, and dexterity. Try dry runs without any media or anything to see how it feels. Just use some empty jars, bottles, and petris.

    To practice and test your SAB skills, try using plane agar plates and grains. Don't inoculate them or anything. Just make some agar and/or grain jars, do some transfers of plain agar, and see if they contaminate. You can do agar to agar, agar to grain, or whatever. You can also practice your G2G this way. If you don't inoculate and you get growth in any of it, then you know it's a contam and you know it's your technique. Without any competing culture, contams should be much easier to see. I've practiced this myself because I got tired of spending money on spores and wasting a lot of time.

One last thing: Always flame sterilize outside the SAB



***Modification Update***
Optional. (I'm fascinated by magnets)

Today, I added a magnetic arm-hole cover to my SAB.



Normally, I cover my arm-holes with a piece of Suran-wrap. I do this during those short periods when I'm kind of in between tasks or taking a break. Some people use those PVC screw on things.

I made these of those magnetic vent covers. It was $6.49 for a pack of 3. They seem to come in different sizes, so, get the size that covers the holes well. It only takes 2 out of the 3-pack. Because my arm-holes are 5 inches, I had to use the 8x15 vent covers.

One side is white, and the other side is bare magnet. I noticed the magnetic sides stick together the best. I also noticed the magnetic force is stronger depending on how you orient the magnets. So, if you just cut them in half, play around with the orientations to see which way is strongest before you make any holes and trace you lines.

Here's what you do:

Cut two of your magnetic vent covers in half. Cut it so it looks closer to being a 2 squares than being 2 long strips.

Tape them over your arm-holes and trace out the arm-hole circle on the magnet. Also, mark the SAB where the edges of the magnet is. This is where you need to sand a little to scruff u the plastic so RTV will stick better.

   

Once you get them cut out, RTV them to the SAB. Before you use adhesive, lay the magnets on the SAB to make sure they line up. It's a bitch to cut with wet RTV. Make whatever adjustments you want.

One of the things I like about this is it requires no special tools. It can be done to any existing SAB that doesn't have arm-hole covers already. You can make the arm-holes any size and this will still work because you don't have have specific hole sizes.


Edited by SpitballJedi (11/17/14 12:08 AM)


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InvisibleCitizen XS
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Re: My SAB (Still Air Box) [Re: SpitballJedi] * 3
    #20048942 - 05/27/14 11:09 PM (6 years, 4 months ago)

Good write up


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Re: My SAB (Still Air Box) [Re: Citizen X] * 2
    #20049281 - 05/28/14 12:41 AM (6 years, 4 months ago)

I dig it
How much did the drill bit cost


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Offlineamungus
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Re: My SAB (Still Air Box) [Re: SpitballJedi] * 2
    #20049314 - 05/28/14 12:46 AM (6 years, 4 months ago)

I use that exact same tote for my SAB :thumbup:
Works like a charm ,good write up man.


--------------------
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InvisibleSpitballJedi
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Re: My SAB (Still Air Box) [Re: Jot] * 1
    #20049330 - 05/28/14 12:51 AM (6 years, 4 months ago)

I don't remember. I've had it for years. I think you can get them for less than $10 in a kit at those cheap places like Harbor Freight and Northern Tool. You only need a cheep one.

I've heard of some people heating up a coffee can on the stove, but I've never tried it.


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Offlineamungus
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Re: My SAB (Still Air Box) [Re: SpitballJedi] * 2
    #20049337 - 05/28/14 12:53 AM (6 years, 4 months ago)

That's how I cut my holes.
I used my stove to heat the bottom of a coffee can and melted holes out.
When I'm using my SAB I use a pair of tyvek sleeves to stuff in the holes.


--------------------
:penis::penis:
"Seems so sick to the hypocrite norm Running their boring drills But we are an elite race of our own The stoners, junkies, and freaks" - Layne Staley


Edited by amungus (05/28/14 12:57 AM)


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Re: My SAB (Still Air Box) [Re: amungus] * 2
    #20049366 - 05/28/14 01:02 AM (6 years, 4 months ago)

nice stuff brutha! glad to see ya back:bongload:


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Re: My SAB (Still Air Box) [Re: cronicr] * 2
    #20049420 - 05/28/14 01:23 AM (6 years, 4 months ago)

i gotta get me one of those.  I'm still using a 66qt tote for g2g :frown:


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Re: My SAB (Still Air Box) [Re: blindingleaf] * 2
    #20049435 - 05/28/14 01:28 AM (6 years, 4 months ago)

i say tag this man, spitball deserves it...or rather the community does:super:


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It doesn't matter what i think of you...all that matters is clean spawn

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Re: My SAB (Still Air Box) [Re: cronicr] * 1
    #20236797 - 07/07/14 01:44 PM (6 years, 3 months ago)

Quote:

cronicr said:
i say tag this man, spitball deserves it...or rather the community does:super:



:whathesaid::takingnotes:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleSpitballJedi
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Re: My SAB (Still Air Box) [Re: cronicr] * 2
    #20236803 - 07/07/14 01:46 PM (6 years, 3 months ago)

Not really. I know the basics. I just seam smart because I only talk about what I know. But I do appreciate the gesture.


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The Basics
A little civility goes a long way

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Re: My SAB (Still Air Box) [Re: SpitballJedi] * 2
    #20236812 - 07/07/14 01:48 PM (6 years, 3 months ago)

:laugh2: i just needed an excuse to bump some of your shit lol, lots of people need teks these days:hehehe:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleSpitballJedi
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Re: My SAB (Still Air Box) [Re: cronicr] * 1
    #20236816 - 07/07/14 01:49 PM (6 years, 3 months ago)

Quote:

cronicr said:
:laugh2: i just needed an excuse to bump some of your shit lol, lots of people need teks these days:hehehe:



:like:


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


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Invisibletoxetel
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Re: My SAB (Still Air Box) [Re: SpitballJedi] * 3
    #20237332 - 07/07/14 04:07 PM (6 years, 3 months ago)

Quote:

SpitballJedi said:
I only talk about what I know.




:seriousthankyou:


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Re: My SAB (Still Air Box) [Re: toxetel] * 2
    #20237677 - 07/07/14 05:36 PM (6 years, 3 months ago)

Your write up are all very well done Spitball. Your's and Frank's How I get Stuff Done are 2 of my favorite bookmarks on here


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Re: My SAB (Still Air Box) [Re: Attainment] * 1
    #20239568 - 07/07/14 11:24 PM (6 years, 3 months ago)

When using the SAB, do you put stuff through the holes or do you lift it up?


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InvisibleSpitballJedi
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Re: My SAB (Still Air Box) [Re: aww] * 1
    #20239595 - 07/07/14 11:33 PM (6 years, 3 months ago)

Sometimes I do G2G and agar in the same sitting. I may lift it and put my grain jars in, then lift it to get them out. Or I may leave them in so I can use them kinda like a shelf and just slip a few already poured agar dishes through the holes.

It just depends on how many things I need to put in it.


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Offlinecinderblock
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Re: My SAB (Still Air Box) [Re: cronicr] * 1
    #20307248 - 07/21/14 08:19 PM (6 years, 3 months ago)

Quote:

cronicr said:
i say tag this man, spitball deserves it...or rather the community does:super:




Agreed, he makes the best teks, hands down.


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Re: My SAB (Still Air Box) [Re: SpitballJedi] * 1
    #20307260 - 07/21/14 08:22 PM (6 years, 3 months ago)

Thanks man!  Going to walmart tomorrow to get me a SAB!


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"Additionally I did one Armageddon jar with all of the types together."


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Offlinecinderblock
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Re: My SAB (Still Air Box) [Re: cinderblock] * 1
    #20307276 - 07/21/14 08:25 PM (6 years, 3 months ago)

You recommended doing all your work towards the back of the box. Do you wear tyvek sleeves?

Also, do you rub everything that goes into the SAB before AND after? Or just after it enters the SAB?

Do you use Dr. Bonner's for a specific reason? Or can I just use aerosol like Oust? Thanks.


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Re: My SAB (Still Air Box) [Re: TheChief]
    #20307282 - 07/21/14 08:27 PM (6 years, 3 months ago)

Quote:

MattDiGi said:
Thanks man!  Going to walmart tomorrow to get me a SAB!




Yeah, I'm gonna go to Target today and look around to stock up. I only get one offday, so this is it for the week.


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Re: My SAB (Still Air Box) [Re: cinderblock]
    #20308100 - 07/21/14 11:02 PM (6 years, 3 months ago)

Quote:

cinderblock said:
You recommended doing all your work towards the back of the box. Do you wear tyvek sleeves?

Also, do you rub everything that goes into the SAB before AND after? Or just after it enters the SAB?

Do you use Dr. Bonner's for a specific reason? Or can I just use aerosol like Oust? Thanks.




yes

I do a quick wipe down before going in the box, and a more thorough wipe while in the box. Not needed after coming out. Do your flame sterilization outside the box though.

I use Dr. Bronner's because it smells good. Yes, you can use oust, but, it doesn't help any more than soap. You just need the air inside to be still and the wet surface helps contams stick if they do come in the box. Aerosols irritate my nose.


--------------------
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A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20309174 - 07/22/14 02:16 AM (6 years, 3 months ago)

Quote:

SpitballJedi said:
Quote:

cinderblock said:
You recommended doing all your work towards the back of the box. Do you wear tyvek sleeves?

Also, do you rub everything that goes into the SAB before AND after? Or just after it enters the SAB?

Do you use Dr. Bonner's for a specific reason? Or can I just use aerosol like Oust? Thanks.




yes

I do a quick wipe down before going in the box, and a more thorough wipe while in the box. Not needed after coming out. Do your flame sterilization outside the box though.

I use Dr. Bronner's because it smells good. Yes, you can use oust, but, it doesn't help any more than soap. You just need the air inside to be still and the wet surface helps contams stick if they do come in the box. Aerosols irritate my nose.




Do you wait at all after you spray the soap for it to settle for a couple minutes? Or does it not really matter that the soap wait is still probably drizzling around at a microscopic level.


Edited by cinderblock (07/22/14 02:43 AM)


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InvisibleSpitballJedi
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Re: My SAB (Still Air Box) [Re: cinderblock]
    #20309930 - 07/22/14 09:18 AM (6 years, 3 months ago)

I get the walls and towel damp, give a couple more light sprays, put my stuff in, wait about 5 minutes, then wipe all my jars and stuff down with alcohol.


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A little civility goes a long way

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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20333887 - 07/26/14 08:32 PM (6 years, 2 months ago)

Added to OP

***Modification Update***


Today, I added a magnetic arm-hole cover to my SAB.



Normally, I cover my arm-holes with a piece of Suran-wrap. I do this during those short periods when I'm kind of in between tasks or taking a break. Some people use those PVC screw on things.

I made these of those magnetic vent covers. It was $6.49 for a pack of 3. They seem to come in different sizes, so, get the size that covers the holes well. It only takes 2 out of the 3-pack. Because my arm-holes are 5 inches, I had to use the 8x15 vent covers.

One side is white, and the other side is bare magnet. I noticed the magnetic sides stick together the best. I also noticed the magnetic force is stronger depending on how you orient the magnets. So, if you just cut them in half, play around with the orientations to see which way is strongest before you make any holes and trace you lines.

Here's what you do:

Cut two of your magnetic vent covers in half. Cut it so it looks closer to being a 2 squares than being 2 long strips.

Tape them over your arm-holes and trace out the arm-hole circle on the magnet. Also, mark the SAB where the edges of the magnet is. This is where you need to sand a little to scruff u the plastic so RTV will stick better.

   

Once you get them cut out, RTV them to the SAB. Before you use adhesive, lay the magnets on the SAB to make sure they line up. It's a bitch to cut with wet RTV. Make whatever adjustments you want.

One of the things I like about this is it requires no special tools. It can be done to any existing SAB that doesn't have arm-hole covers already. You can make the arm-holes any size and this will still work because you don't have have specific hole sizes.


--------------------
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A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Edited by SpitballJedi (07/27/14 06:38 PM)


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Invisiblemushroomama
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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20334640 - 07/26/14 10:49 PM (6 years, 2 months ago)

I wasn't able to acquire a hole saw, and after trying several different cutting methods to make my arm holes, and failing miserably at each, I went out and bought a soldering iron and it worked like a champ. I used a fan to circulate air as I cut the holes. No sharp edges, no broken exacto blades, box cutters slipping, or dozens of tiny drill holes that led to cracking. I have been trying to figure out an easy cover for my arm holes. I picked up some magnetic sheets at the dollar tree today after seeing this and am going to use them to make covers! Thanks for the great idea!


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"Now I know what the picture puzzled pattern door is. The picture puzzle pattern door is the furthest reaches of your humanity. This is the doorway into what defines you as a human being."


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Re: My SAB (Still Air Box) [Re: mushroomama]
    #20337476 - 07/27/14 02:33 PM (6 years, 2 months ago)

Quote:

mushroomama said:
I wasn't able to acquire a hole saw, and after trying several different cutting methods to make my arm holes, and failing miserably at each, I went out and bought a soldering iron and it worked like a champ. I used a fan to circulate air as I cut the holes. No sharp edges, no broken exacto blades, box cutters slipping, or dozens of tiny drill holes that led to cracking. I have been trying to figure out an easy cover for my arm holes. I picked up some magnetic sheets at the dollar tree today after seeing this and am going to use them to make covers! Thanks for the great idea!



You can heat a razorblade with a torch until red hot, slowly cut the traced circle bit by bit.  I found this to be the cheapest, most effective and safe method for cutting holes in tubs.  Just be careful and take your time.


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"Additionally I did one Armageddon jar with all of the types together."


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Re: My SAB (Still Air Box) [Re: TheChief]
    #20337527 - 07/27/14 02:40 PM (6 years, 2 months ago)

Some people heat up a coffee can to melt the holes too.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20337558 - 07/27/14 02:43 PM (6 years, 2 months ago)

Quote:

SpitballJedi said:
Some people heat up a coffee can to melt the holes too.



I thought about it, but I used those stupid PVC things and finding the right sized coffee can would have been a struggle.  I ended up just doing the razorblade method and it worked awesome.


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"Additionally I did one Armageddon jar with all of the types together."


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Re: My SAB (Still Air Box) [Re: TheChief]
    #20337830 - 07/27/14 03:25 PM (6 years, 2 months ago)

What's everyone's thoughts on cutting a third, smaller hole on the side of the box for flame sterilization? A hole just big enough to stick a needle through and make contact with a flame from a torch.


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Re: My SAB (Still Air Box) [Re: gannondworf]
    #20337840 - 07/27/14 03:27 PM (6 years, 2 months ago)

to close for my comfort


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Re: My SAB (Still Air Box) [Re: gannondworf]
    #20337873 - 07/27/14 03:31 PM (6 years, 2 months ago)

Pointless at best. It's been done.

Taking your hand in and out of a SAB for flame sterilizing has been done with great success by many many people for a really long time.


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Re: My SAB (Still Air Box) [Re: TheChief]
    #20338929 - 07/27/14 07:16 PM (6 years, 2 months ago)

Quote:

TheChief said:
Quote:

mushroomama said:
I wasn't able to acquire a hole saw, and after trying several different cutting methods to make my arm holes, and failing miserably at each, I went out and bought a soldering iron and it worked like a champ. I used a fan to circulate air as I cut the holes. No sharp edges, no broken exacto blades, box cutters slipping, or dozens of tiny drill holes that led to cracking. I have been trying to figure out an easy cover for my arm holes. I picked up some magnetic sheets at the dollar tree today after seeing this and am going to use them to make covers! Thanks for the great idea!



You can heat a razorblade with a torch until red hot, slowly cut the traced circle bit by bit.  I found this to be the cheapest, most effective and safe method for cutting holes in tubs.  Just be careful and take your time.



I did try that method and was unsuccessful. Then I tried an exacto blade with the same approach, and again failed miserably. The soldering iron was just under $8 at Walmart. I cut both holes in about 10 minutes.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20339433 - 07/27/14 08:50 PM (6 years, 2 months ago)

SBJ That magnetic things are a wonderful idea. I hate my pcv plugs. Also i need to get one of those 108 quart tubs.

How do you clean and how often you clean your towel?  mine i just damp it in bleach water.  But then it smells funny...


Edited by Mr. Alien (07/27/14 08:51 PM)


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Re: My SAB (Still Air Box) [Re: gannondworf]
    #20339477 - 07/27/14 08:59 PM (6 years, 2 months ago)

Quote:

gannondworf said:
What's everyone's thoughts on cutting a third, smaller hole on the side of the box for flame sterilization? A hole just big enough to stick a needle through and make contact with a flame from a torch.



I did this with my first SAB and the flame ignited the alcohol fumes inside the box creating a huge fire ball that melted my tyvek sleeves to my arms.


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Re: My SAB (Still Air Box) [Re: MudaFuka]
    #20339530 - 07/27/14 09:11 PM (6 years, 2 months ago)

Fuck. I've melted gloves on my hands before.


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Re: My SAB (Still Air Box) [Re: Mr. Alien]
    #20339599 - 07/27/14 09:28 PM (6 years, 2 months ago)

Quote:

Mr. Alien said:
SBJ That magnetic things are a wonderful idea. I hate my pcv plugs. Also i need to get one of those 108 quart tubs.

How do you clean and how often you clean your towel?  mine i just damp it in bleach water.  But then it smells funny...




I use a fresh towel for ever project. Once I'm done for the day, I remove the SAB and towel goes in the laundry. If you leave your SAB closed up on the wet towel it will mildew. Bleach won't stop it.

Because of my high contamination rate in the early days, I still have some phobias and extra precautions. I have 3 white towels I bought special for my SAB. They get washed and dried separate from all my other laundry. Once dry, they go in a garbage bag to keep dust from settling on them.

When I use it, I lay out a folded towel and spray it with soap water and place my IPA sanitized box on it.

I have soap water in one bottle and IPA in another. I use them both at different points. Soap water can make your jars, lids, and gloves slippery, but IPA is flammable so I try to only use it for wiping stuff down.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20340032 - 07/27/14 10:52 PM (6 years, 2 months ago)

I just made a modification to my new SAB

I added steel grating three and a half inches up from the base so nothing will com into contact with the towel I put at the bottom to catch contams.


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Re: My SAB (Still Air Box) [Re: MudaFuka]
    #20340214 - 07/27/14 11:28 PM (6 years, 2 months ago)

Thanks jedi, i will do the same.

Moda your wood is painted with some outdoor paint for wood?


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Re: My SAB (Still Air Box) [Re: Mr. Alien]
    #20340304 - 07/27/14 11:45 PM (6 years, 2 months ago)

I haven't painted the wood yet but I plan on putting sealer on it before I use it to many times.


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Re: My SAB (Still Air Box) *DELETED* [Re: SpitballJedi]
    #20343229 - 07/28/14 05:08 PM (6 years, 2 months ago)

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Re: My SAB (Still Air Box) [Re: Sanguin3]
    #20344040 - 07/28/14 08:55 PM (6 years, 2 months ago)

Great minds must think alike

http://www.shroomery.org/forums/showflat.php/Number/20337941#20337941
Quote:

SpitballJedi said:
In an attempt to make Rube Goldberg proud, I just had an idea.

What if I applied my magnetic SAB arm-hole cover idea to your ironed poly idea?

Instead of sticking the poly to the tub, stick it to a piece of magnetic vent cover. Attach a piece of vent cover with a hole in it to the tub. While colonizing, just put a solid piece over the hole and switch when time to fruit. No more tape, reusable, easy to remove filter to wash.

Bonkers, right?




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Re: My SAB (Still Air Box) [Re: SpitballJedi] * 1
    #20344077 - 07/28/14 09:09 PM (6 years, 2 months ago)

I know some of you have seen this, but I'll share again...



The arm holes are made from plastic tubs with the bottoms cut off. I used a grinder with a very fine blade to precision cut the holes for exact fit, no glue at all.

When I want to use it I give it a wipe with bleach and then spray it full of Glen20 (like Oust I think, kills %99.9 of everything) then stick the covers back on. 1 hour later it is ready for me. Highly sanitized.


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Re: My SAB (Still Air Box) [Re: Northerner]
    #20344572 - 07/28/14 11:13 PM (6 years, 2 months ago)

Where do you get oust, i though that thing was discontinued... :frown:


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Re: My SAB (Still Air Box) [Re: Mr. Alien]
    #20344649 - 07/28/14 11:34 PM (6 years, 2 months ago)

I dunno, I live in Australia. We have Glen20 here. There must be an equivalent anti-bacterial spray in your part of the world. Look in the cleaning section in the supermarket.


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Re: My SAB (Still Air Box) [Re: Northerner]
    #20344703 - 07/28/14 11:55 PM (6 years, 2 months ago)

In Canada I cant find any kind of air sanitizer sprays.


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Re: My SAB (Still Air Box) [Re: MudaFuka]
    #20344859 - 07/29/14 12:51 AM (6 years, 2 months ago)

Some crazy Northerner probably tried to snort the shit and killed himself, got it banned.

There isn't actually any use for the stuff though except this, to my knowledge. I'm surprised people buy it.


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Re: My SAB (Still Air Box) [Re: Northerner]
    #20344866 - 07/29/14 12:53 AM (6 years, 2 months ago)

It was probably something like that. I know they used to sell it back in the 90s but I haven't seen it recently.


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Re: My SAB (Still Air Box) *DELETED* [Re: Northerner]
    #20344902 - 07/29/14 01:13 AM (6 years, 2 months ago)

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Re: My SAB (Still Air Box) [Re: Sanguin3]
    #20344986 - 07/29/14 01:59 AM (6 years, 2 months ago)

We should sign a petition or something for getting back oust to the market. :fuckinawesome:


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Re: My SAB (Still Air Box) [Re: Mr. Alien] * 1
    #20345026 - 07/29/14 02:15 AM (6 years, 2 months ago)

I use this, Lysol Neutra Air.


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Re: My SAB (Still Air Box) [Re: Mr. Alien]
    #20345039 - 07/29/14 02:20 AM (6 years, 2 months ago)

:whathesaid: that's the stuff      actually just got a good whif of it now... That's the smell of success  Wish me luck


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Edited by tombosley8 (07/29/14 02:26 AM)


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Re: My SAB (Still Air Box) [Re: MudaFuka]
    #20345053 - 07/29/14 02:24 AM (6 years, 2 months ago)

Quote:

MudaFuka said:
In Canada I cant find any kind of air sanitizer sprays.



there's lots here:shrug:


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Re: My SAB (Still Air Box) [Re: cronicr]
    #20345427 - 07/29/14 05:44 AM (6 years, 2 months ago)

Quote:

cronicr said:
Quote:

MudaFuka said:
In Canada I cant find any kind of air sanitizer sprays.



there's lots here:shrug:




I was just gonna say that, ask cronicr. He'll know what's up :smile:
took me a while to get all the stuff needed for this hobby here in Belgium.
I found the disinfectant spray in the hardware store in the cleaning section...


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Re: My SAB (Still Air Box) [Re: cronicr]
    #20345681 - 07/29/14 08:45 AM (6 years, 2 months ago)

Quote:

cronicr said:
Quote:

MudaFuka said:
In Canada I cant find any kind of air sanitizer sprays.



there's lots here:shrug:




...and not needed. Just about any aerosol works. At best you're just reducing the spore load a little.

Sure spray the air in your room with whatever so the wet vapors drag some of the particles down; it's the same thing inside the SAB. You can use plain water in a spray bottle and get the same results as air sanitizer.

But you're main focus should be creating a still air environment. Air sanitizers and what not may help a little, but not nearly as much as still air. Air sanitizers just make you feel better because of the bold print on the can; stop wasting money. You don't need anything special.

Truth be told, I have some allergies. I have a big HEPA I often run in my apartment during certain times of year. I move it in to my work room a couple of hours before I do sterile work and turn it off about 30 mins before I start work. Does it help? Maybe a little. Same reason people run their flowhoods a couple hours before they start working.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20345722 - 07/29/14 09:04 AM (6 years, 2 months ago)

Quote:

SpitballJedi said:
Quote:

cronicr said:
Quote:

MudaFuka said:
In Canada I cant find any kind of air sanitizer sprays.



there's lots here:shrug:




...and not needed. Just about any aerosol works. At best you're just reducing the spore load a little.

Sure spray the air in your room with whatever so the wet vapors drag some of the particles down; it's the same thing inside the SAB. You can use plain water in a spray bottle and get the same results as air sanitizer.

But you're main focus should be creating a still air environment. Air sanitizers and what not may help a little, but not nearly as much as still air. Air sanitizers just make you feel better because of the bold print on the can; stop wasting money. You don't need anything special.

Truth be told, I have some allergies. I have a big HEPA I often run in my apartment during certain times of year. I move it in to my work room a couple of hours before I do sterile work and turn it off about 30 mins before I start work. Does it help? Maybe a little. Same reason people run their flowhoods a couple hours before they start working.




I get your point and all but I'd think the benefit of using air sanitizers is that if you'd stir up the air in you SAB 99.99% of the germs are dead?


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Re: My SAB (Still Air Box) [Re: Haywire]
    #20345778 - 07/29/14 09:28 AM (6 years, 2 months ago)

Not exactly. No scientific evidence for the products claimed efficacy has to be provided by the manufacturer in the US. In other words, the can says, in big bold letters "Kills", but that may or may not actually be true and they don't have to prove it. Like Flava Flav says: "Don't believe the hype"


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20345808 - 07/29/14 09:43 AM (6 years, 2 months ago)

Quote:

SpitballJedi said:
Not exactly. No scientific evidence for the products claimed efficacy has to be provided by the manufacturer in the US. In other words, the can says, in big bold letters "Kills", but that may or may not actually be true and they don't have to prove it. Like Flava Flav says: "Don't believe the hype"




that is wrong in so many ways. I'm not in the us though... perhaps we have better sanitizers :smile:

I will put your thesis to the test! unless you already have...


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Re: My SAB (Still Air Box) [Re: Haywire]
    #20345925 - 07/29/14 10:56 AM (6 years, 2 months ago)

I found this :shrug:

Quote:

RogerRabbit said:
To answer your question, oust DOES kill airborne bacteria.  I've proved this time and time again with petri dish tests before and after spraying.  Open a petri for ten seconds before spraying with oust, and it grows all kinds of bacteria.  Open a petri for ten seconds after two oust treatments ten minutes apart, and NO bacteria grows in the petri dish.  Couple an oust treatment like that with what Agar said above, and you'll have pretty good success.  Lysol is a SURFACE disinfectant and does nothing for the air.  Oust is designed to remain as a fog for several minutes, thus sanitizes the air.
RR




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Re: My SAB (Still Air Box) [Re: Mr. Alien] * 1
    #20345970 - 07/29/14 11:25 AM (6 years, 2 months ago)

I don't know if it makes a difference or not, but I use it anyways. You can never be too sterile IMO..and it only takes an extra minute. I'm sure it would be fine if I didn't use it, but why change what's working?


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Re: My SAB (Still Air Box) [Re: wowimflabbergasted]
    #20346159 - 07/29/14 12:30 PM (6 years, 2 months ago)

You're right, it doesn't really hurt to be a little extra cautious. Air sanitizers are not completely ineffectual, they're just not as useful as people think they are.

Even if they kill 99.99% of the contams, you still introduce more as everything settles. You bring them in when you come back into the room or put your hands in your SAB. Still air prevents the additional contams from getting in to your sterile work.

I can't remember the last time I bought an air sanitizer. Reducing your spore load is a good thing, but still air is more important. That's all the point I'm really trying to make.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20346297 - 07/29/14 01:16 PM (6 years, 2 months ago)

Yeah, even if you don't clean your SAB surfaces and air sanitise inside. you can get away with it.


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Re: My SAB (Still Air Box) [Re: Mr. Alien]
    #20387725 - 08/07/14 02:27 PM (6 years, 2 months ago)

Is there any worries about the towel in the SAB? I know that towels are like contam traps and are usually loaded with bacteria, are they not? Does sterilizing the box kill all the bacteria?


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Re: My SAB (Still Air Box) [Re: jordano]
    #20387767 - 08/07/14 02:38 PM (6 years, 2 months ago)

Sure You can sterilise a SAB, you just need your sab to be made of polypropylene plastic and you would need a steriliser big enough for get the SAB in.

Maybe you mean Sanitize?


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Re: My SAB (Still Air Box) [Re: Mr. Alien]
    #20388068 - 08/07/14 03:55 PM (6 years, 2 months ago)

Yes, the towel is usually contaminated. But, since it's damp, contams get trapped. I fold the towel and can get a couple of uses out of it before I use a freshly laundered one.

The SAB does not need, nor is it, sterile. The air is just still.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20400216 - 08/10/14 07:21 PM (6 years, 2 months ago)

thanks for the insights, makes more sense to me now. and sorry for using the wrong word mr sarcastic :tongue: :cool:


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Re: My SAB (Still Air Box) [Re: jordano]
    #20400319 - 08/10/14 07:55 PM (6 years, 2 months ago)

Hahaha just a healthy sarcastic joke :P. :hi:


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Re: My SAB (Still Air Box) [Re: Mr. Alien]
    #20416098 - 08/14/14 02:59 AM (6 years, 2 months ago)

I had a few questions.

1) How do you insert the quart jars into your SAB? Do you just lift the tote and put everything in first? Or do you somehow angle it in through the arm holes?

2) Why do you need to cover the SAB when it's not in use? Don't you just have to spray it beforehand?


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Re: My SAB (Still Air Box) [Re: cinderblock]
    #20416103 - 08/14/14 03:04 AM (6 years, 2 months ago)

1)i lift(my tote is upsidedown)
2)ocd:shrug:


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Re: My SAB (Still Air Box) [Re: cinderblock]
    #20418770 - 08/14/14 07:03 PM (6 years, 2 months ago)

Quote:

cinderblock said:
I had a few questions.

1) How do you insert the quart jars into your SAB? Do you just lift the tote and put everything in first? Or do you somehow angle it in through the arm holes?

2) Why do you need to cover the SAB when it's not in use? Don't you just have to spray it beforehand?




1) it depends. If I'm putting a few things in, I lift it. If I'm just grabbing 1 or two things, I stick it through the hole.

2) Sometimes I need a break from what I'm doing and leave the room. When If I need to go out of the room a few times, I just feel better knowing stuffs not blowing in. But, some OCD, but little effort for a lot of warm fuzzy feeling.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20419276 - 08/14/14 08:44 PM (6 years, 2 months ago)

Quote:

SpitballJedi said:
Quote:

cinderblock said:
I had a few questions.

1) How do you insert the quart jars into your SAB? Do you just lift the tote and put everything in first? Or do you somehow angle it in through the arm holes?

2) Why do you need to cover the SAB when it's not in use? Don't you just have to spray it beforehand?




1) it depends. If I'm putting a few things in, I lift it. If I'm just grabbing 1 or two things, I stick it through the hole.

2) Sometimes I need a break from what I'm doing and leave the room. When If I need to go out of the room a few times, I just feel better knowing stuffs not blowing in. But, some OCD, but little effort for a lot of warm fuzzy feeling.




Thanks, will knock up for the first time later tonight. Hopefully it won't get contaminated.


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Re: My SAB (Still Air Box) [Re: cinderblock]
    #20419377 - 08/14/14 09:01 PM (6 years, 2 months ago)

Don't get discouraged if it does. Some stuff takes a few tries. Just stick with what we know works and get the details ironed out.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20839571 - 11/15/14 06:42 AM (5 years, 11 months ago)

Bump.

The first page could use a good SAB tek, figured I'd send this one up there since I was reviewing this after I got myself a new SAB (happens to be the same one as you have, SBJ)

The specific reason I ran over the thread was because I was wondering about your towel under the SAB thing.  Have you done any experimenting with that that seems to confirm that having the towel there is definitely more effective than just working with a flat wet floor?

I'm also thinking about arm hole covers but I don't really want to do the magnetic ones.  At first I was enticed by the PVC plugs but now I don't know what I want to do.

I'm excited that I get to convert my old 19 gallon SAB into a mono now, though.

Hey Alien why do you hate your PVC arm holes?


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #20840096 - 11/15/14 11:34 AM (5 years, 11 months ago)

I have noticed a positive difference since I started using a towel. I have never used hole covers of any kind and it hasn't been a problem for me.


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Re: My SAB (Still Air Box) [Re: MudaFuka]
    #20840214 - 11/15/14 12:08 PM (5 years, 11 months ago)

Thx for the bump.

I don't always use the covers and had none for a while. I haven't noticed any difference really in contam rates since they were added. I just think magnets are magical...lol. But,I honestly don't think covers of any kind are needed.

There is no empirical evidence that the towel has improved my contam rate. But, there is some solid logic and my experience to support it. My contam rate has drastically improved since I flipped the SAB upside down and I've always used the towel. (thx NH420)

Just using a wet tabletop would probably work. The rim of the tub is not perfect and will allow air in through the imperfect seal. If it is wet around the seal, I would think many contams would get trapped. Depending on what you use to wet the surface, a small amount of air flow may dry it out fast, thus no more trap. On the other hand, there really shouldn't be much, if any, air flow in a SAB.

Maybe I'm a super heater, but my hands and arms seam to produce enough heat in my SAB to get a thin layer of condensation on the SAB surface if I'm doing a lot of work. This is compounded when pouring warm agar. Although it's not much, I imagine it's enough to create some measurable amount of air circulation. I can't say it's enough to cause an issue with an imperfect seal or not.

My table started getting a bow in the middle and the towel wasn't enough to keep a seal. I discovered this while looking over my routine trying to identify the source of a contam. I know contams happen even when we do everything perfectly, but I bought a big old glass table top from the thrift store for $5 bucks and haven't had a contam since. Could be coincidence though.

I've thought about ditching the towel, too. But, I'm afraid because my SAB set-up has worked so well.

Try it without the towel and let us know. If it seems to work for you, I'll give it a shot too.


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Edited by SpitballJedi (11/15/14 12:09 PM)


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20840506 - 11/15/14 01:18 PM (5 years, 11 months ago)

I've never used a towel and I've never had a contaminated spawn jar.


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Re: My SAB (Still Air Box) [Re: wowimflabbergasted]
    #20840520 - 11/15/14 01:23 PM (5 years, 11 months ago)

What about plates?


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Re: My SAB (Still Air Box) [Re: MudaFuka]
    #20840558 - 11/15/14 01:32 PM (5 years, 11 months ago)

Only a few of my most recent B+ plates from an old print. It wasn't the most sterile print because I took it 3-4 years ago when I had no idea what I was doing, so that was the problem. I was able to get some good sectors though and have 5 oat jars with agar plates in them right now. :rockon:


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Re: My SAB (Still Air Box) [Re: wowimflabbergasted]
    #20840799 - 11/15/14 02:26 PM (5 years, 11 months ago)

Well I don't have a hole saw so I may have to figure something out instead.  Someone mentioned using a soldering iron but I really actually have to use that to solder sometimes... I suppose I could just cut the circles out with a hot blade.


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #20840938 - 11/15/14 02:58 PM (5 years, 11 months ago)

I used an exacto blade to score the plastic then a pair of tin nips to cut it. You need to be pretty good with a pair of snips not to crack it though.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20847334 - 11/16/14 08:41 PM (5 years, 11 months ago)

I updated the OP with the following:


Getting Ready For Doing Work

I lay the towel down on the table. I have large bath sized towels, so I fold it over. Then, I spray the towel with soap water. I usually use Dr Bronner's Almond Scented Magic Soap. It's what I shower with, but any soap will do. It doesn't take much, just a couple of drops per liter of water. The soap just keeps the water from evaporating too quickly and gives it a little stickiness. I don't see the point in sanitizing the table because it's going to have a towel over it, but do it if it makes you feel good.

Then, I clean the walls of the SAB with 70% IPA. After that, I give a spritz of soap water on the walls and turn the SAB upside down on the towel. Contaminants will stick to wet surfaces. I find soapy water works best. Bleach irritates my nose and lungs. Alcohol dries fast and is flammable.

You can either have the jars or whatever on the towel and cover with the box or feed everything through the arm-holes; it doesn't matter at all.

Once my stuff is in, I let everything just settle for a few minutes, maybe 2-5 minutes. Then, I wipe all my jars or whatever with 70% IPA.

Tips


    As Already mentioned, the point of a SAB is to have a place of still air to do sterile work. We call it sterile work because you want to keep your sterilized stuff sterile. The inside of the SAB is not sterile, just still. I put the SAB in the least drafty room, close the door, and turn off anything that creates drafts or air circulation.

Let things settle before wiping down jars in your SAB.

    I wear nitrile exam gloves and tyvek sleeves. I actually wear the top half of a tvek suit with the legs cut off. You can just wear a clean long-sleeve shirt though. When not in use, I keep the tyvek in a plastic bags just to keep the dust off. I actually keep a lot of stuff wrapped up in bags or totes to keep the dust off.

    Do all flame sterilizations outside the SAB. Heat from the flame creates enough current inside the SAB to cause contams to float around and get in to your sterile work.

    It's a fact, attaching gloves to your SAB turns it in to a Glove-box. Attaching the gloves causes more air to move around so therefore, by definition, it is no longer a Still Air Box. I'm sure there are all kinds of ways to prevent contaminated air from coming in to your GB, but you better make sure the inside is already sterile or it will be pointless. Good luck getting it sterile.

    Do your work toward the back of the SAB to help prevent contams from getting in your sterile work via the arm holes.

    Work diligently. Don't get in a hurry or work at a snails pace. Make all your movements deliberately. Getting in a hurry can stir up the air inside. Working too slow leaves you open for higher potential of getting contams.

Crack the lids on jars before you put them in the SAB. A stuck lid is a bitch to open inside it.

You don't have to shower the walls. You just want a little spritz. Too much, especially in front, will make it hard to see what you're doing. If you are doing much work or working with warm agar, you'll notice condensation building up on the walls anyway. There shouldn't be many contams floating around and it doesn't take much to trap them.

Keep movement over open sterile containers at a bare minimum. I don't like to sit anything dow. This takes a little practice, planning, and dexterity. Try dry runs without any media or anything to see how it feels. Just use some empty jars, bottles, and petris.

One last thing: Always flame sterilize outside the SAB


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20848040 - 11/16/14 11:18 PM (5 years, 11 months ago)

Good addition :highfive1:

I couldn't agree more with cracking the lids before working. I've remade my lids upside down though so this isn't a problem, but I do find it harder to keep the lids from sliding around on the jar this way.


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Re: My SAB (Still Air Box) [Re: wowimflabbergasted]
    #20848115 - 11/16/14 11:37 PM (5 years, 11 months ago)

What I meant by cracking the lid was actually unscrew it a little. I guess I'll clarify that in the OP.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20848119 - 11/16/14 11:38 PM (5 years, 11 months ago)

Ah okay I got ya. Cracking the lids outside the SAB isn't a good idea lol that's why I quit.


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Re: My SAB (Still Air Box) [Re: wowimflabbergasted]
    #20848127 - 11/16/14 11:42 PM (5 years, 11 months ago)

I pull my rings all off once they're inside the SAB and then unseal the lid of the jar(s) I'm about to work with, is there anything wrong with that?


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Re: My SAB (Still Air Box) [Re: wowimflabbergasted]
    #20848137 - 11/16/14 11:46 PM (5 years, 11 months ago)

:lol: Yeah, I clarified. I don't use the 2-piece metal ones anymore, so I forgot. Good call and thanks for bringing it up. I wonder how many noobs would be cracking the lid part and trying to fogure out where the contams were coming from.

Quote:

SBJ said: Crack the lids.




Here's the update to the OP:

Crack the lids on jars before you put them in the SAB. A stuck lid is a bitch to open inside it. What I mean is, barely unscrew the metal ring or plastic lid so you don't have to fight it inside the box. If using the 2 piece metal lids, don't try to break the seal on the lid part, just barely loosen the ring. Once they are all wiped down and I'm ready to actually start working, I like to loosen all the lids so that I can finish removing them with one hand.


Edited by SpitballJedi (11/16/14 11:50 PM)


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20848142 - 11/16/14 11:48 PM (5 years, 11 months ago)

Excellent, you use the plastic lids then?


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #20848161 - 11/16/14 11:55 PM (5 years, 11 months ago)

Quote:

Inocuole said:
I pull my rings all off once they're inside the SAB and then unseal the lid of the jar(s) I'm about to work with, is there anything wrong with that?




Sounds right. I just wasn't thinking when I posted. Sorry for the confusion.

And yes, I use plastic now. It's awesome. Lids


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #20848652 - 11/17/14 02:28 AM (5 years, 11 months ago)

Quote:

Inocuole said:
Well I don't have a hole saw so I may have to figure something out instead.  Someone mentioned using a soldering iron but I really actually have to use that to solder sometimes... I suppose I could just cut the circles out with a hot blade.




I traced arm holes then cut them out with a dremel. Worked fine for me just took a little while :shrug:.


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Re: My SAB (Still Air Box) [Re: ToxicDuck]
    #20848662 - 11/17/14 02:33 AM (5 years, 11 months ago)

Quote:

ToxicDuck said:
Quote:

Inocuole said:
Well I don't have a hole saw so I may have to figure something out instead.  Someone mentioned using a soldering iron but I really actually have to use that to solder sometimes... I suppose I could just cut the circles out with a hot blade.




I traced arm holes then cut them out with a dremel. Worked fine for me just took a little while :shrug:.



Be careful of the fumes when melting plastic. That shit is horrible for you. Very...Toxic  :biggrin:


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Re: My SAB (Still Air Box) [Re: wowimflabbergasted]
    #20848754 - 11/17/14 03:17 AM (5 years, 11 months ago)

Quote:

wowimflabbergasted said:
Quote:

ToxicDuck said:
Quote:

Inocuole said:
Well I don't have a hole saw so I may have to figure something out instead.  Someone mentioned using a soldering iron but I really actually have to use that to solder sometimes... I suppose I could just cut the circles out with a hot blade.




I traced arm holes then cut them out with a dremel. Worked fine for me just took a little while :shrug:.



Be careful of the fumes when melting plastic. That shit is horrible for you. Very...Toxic  :biggrin:





Pshh, that'd bother me if I was a pussy. But as you can tell, I'm not a cat, I'm a fucking duck.

Ducks don't give a shit about anything.
Ducks will fuck you up.

:lol:.


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Re: My SAB (Still Air Box) [Re: wowimflabbergasted]
    #20848762 - 11/17/14 03:21 AM (5 years, 11 months ago)

Ugh I was trying to cut the circles in mine and it fucking cracked.  One 13 dollar tub down the shitter.  Well sorta.  I still gotta use it for now, so I put some clear industrial adhesive on it to form a seal.  :shrug:

I guess before I do my next one I have to invest in a big ass hole saw.


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #20848765 - 11/17/14 03:23 AM (5 years, 11 months ago)

Quote:

Inocuole said:
Ugh I was trying to cut the circles in mine and it fucking cracked.  One 13 dollar tub down the shitter.  Well sorta.  I still gotta use it for now, so I put some clear industrial adhesive on it to form a seal.  :shrug:

I guess before I do my next one I have to invest in a big ass hole saw.





You can still crack it with a hole saw, so you still gotta be careful. Go slow,  and have some wood pushed up behind the part you're drilling.


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Re: My SAB (Still Air Box) [Re: ToxicDuck]
    #20848766 - 11/17/14 03:24 AM (5 years, 11 months ago)

Well next time I go to cut it I'm going to point my heat gun at it for a couple seconds to soften it up real good.  That should help right?


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #20848798 - 11/17/14 03:54 AM (5 years, 11 months ago)

Quote:

Inocuole said:
Well next time I go to cut it I'm going to point my heat gun at it for a couple seconds to soften it up real good.  That should help right?





That should help a bit. Since you have a heat gun you probably already know this, but figure I'd say it for anyone else who also has one.


You can use your heat gun to make plastic that isn't crystal clear much more clear. Just point the heat gun at the area you want to be more clear, and keep moving the heat gun back and forth like you're blow drying your hair.


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Re: My SAB (Still Air Box) [Re: ToxicDuck]
    #20848801 - 11/17/14 03:57 AM (5 years, 11 months ago)

That's a pretty fuckin neat tip, I may try it around my viewing area so I have a place I can put my eyes to see real good. 
:vaped:


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #20848848 - 11/17/14 04:32 AM (5 years, 11 months ago)

Quote:

Inocuole said:
That's a pretty fuckin neat tip, I may try it around my viewing area so I have a place I can put my eyes to see real good. 
:vaped:




It helps a lot. Just be careful not to melt the plastic :smile:.


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Re: My SAB (Still Air Box) [Re: ToxicDuck]
    #20849764 - 11/17/14 12:02 PM (5 years, 11 months ago)

Wow good tip Duck, I'll try it and see if it helps. Sometimes it's hard to see in there.

You can also use a coffee can to make the holes. Take an empty coffee can, the bigger ones, heat it up on the stove and push it into the tote where you want your arm holes.


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Re: My SAB (Still Air Box) [Re: ToxicDuck]
    #20849775 - 11/17/14 12:05 PM (5 years, 11 months ago)

Hole-saws can be found for pretty cheap. You can also run the hole-saw in reverse so it doesn't catch and crack the plastic.


Some people heat up a coffee can and melt the holes. I've never tried it though.

If it cracks, tape works fine as a fix. The thing don't need to be water proof or completely air tight. You just want to eliminate as much air movement as possible without creating any vacuums.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20851148 - 11/17/14 05:10 PM (5 years, 11 months ago)

I applied clear industrial adhesive to both sides of the crack and it's pretty much sealed up now, just gotta get on with the arm hole cutting today.  I would do it at night but all the drilling and noise I make probably pisses off the neighbors.

My original design involved cutting 5  1 1/4 inch holes (one in the center, 4 in compass directions) so that I have the general outline of a circle shape already there.  Just gotta cut between the circles.  Somehow I fucked that up too, so heat gun it is, today.

Also my coffee is in a bag.  :shrug:


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #20851874 - 11/17/14 07:30 PM (5 years, 11 months ago)

I added a completely clear viewing window to my SAB using weather stripping.


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Re: My SAB (Still Air Box) [Re: MudaFuka]
    #20852098 - 11/17/14 08:10 PM (5 years, 11 months ago)

Just a quick tip for anyone that has troubles cutting armholes in your SAB...

I know not too many people have them laying around, but sheet metal snips work like a champ. You do have to start holes to get your cut started one way or another... a step-bit works great for that, and are super handy to have around anyways.

But yeah, that's how I did mine, and took only a couple mins, without dealing with plastic fumes or cracking the plastic.


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Re: My SAB (Still Air Box) [Re: Oregonic]
    #20852108 - 11/17/14 08:12 PM (5 years, 11 months ago)

I use tin snips to cut my holes as well.


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Re: My SAB (Still Air Box) [Re: MudaFuka]
    #20852137 - 11/17/14 08:20 PM (5 years, 11 months ago)

Tin snips ftw :thumbup::rockon:


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Re: My SAB (Still Air Box) [Re: Oregonic]
    #20853873 - 11/18/14 02:25 AM (5 years, 11 months ago)

I used an angle grinder with a very fine blade and just cut the holes out on traced lines... turned out perfect.


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Re: My SAB (Still Air Box) [Re: Oregonic]
    #20854270 - 11/18/14 06:37 AM (5 years, 11 months ago)

Quote:

Oregonic said:
Just a quick tip for anyone that has troubles cutting armholes in your SAB...





A hot tin can melt right through plastic tubs if you find one to your size. Everyone has a can laying around.

(just realized that was already said.  i need to go back to sleep hurpadurp)


Edited by stevo (11/18/14 06:42 AM)


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #21100399 - 01/11/15 10:31 AM (5 years, 9 months ago)

Great write up! I've been working in a hood I borrowed from my friend, but unfortunately he needs it back :sad:

Going to make a SAB today and get my hands on some magnets to try that out too! Looks like a fun project. Maybe even use magnets for my monos :awesome:


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Re: My SAB (Still Air Box) [Re: Guardian187]
    #21100408 - 01/11/15 10:33 AM (5 years, 9 months ago)

PS definitely going to practice some agar without inoculating as the hood has been a luxury and probably afforded me some less than perfect technique.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #21377707 - 03/08/15 12:51 PM (5 years, 7 months ago)

Boy I tell you what, that's a nice box. Mmhmm..


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #21381555 - 03/09/15 08:55 AM (5 years, 7 months ago)

Quote:

SpitballJedi said:
Not really. I know the basics. I just seam smart because I only talk about what I know. But I do appreciate the gesture.




One of the smartest sentences on this site.:takingnotes: ty sbj.


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Re: My SAB (Still Air Box) [Re: randoman]
    #21381785 - 03/09/15 10:49 AM (5 years, 7 months ago)

I pimped my SAB with magnet covers too and it's so nice.  I am glad because I originally only had a 6" hole saw Layin aroun when I made my SAB and none of the home hardware stores carry over 4" PVC and I didnt really want to deal with a supply house just to try and find some Shit to cover my holes, so the magnets worked perfectly.  Got a few extra magnets if anyone wants to mod their SAB like this in exchange for some prints!


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Re: My SAB (Still Air Box) [Re: Big Bear]
    #21454735 - 03/25/15 10:36 AM (5 years, 6 months ago)

Locally the only saw and arbor I could find together came to 50 bucks, but I'm drilling two holes in thin plastic. Can't even tell they were used. So if your savvy you can drill the holes for free.


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Re: My SAB (Still Air Box) [Re: Grefa]
    #21454755 - 03/25/15 10:44 AM (5 years, 6 months ago)

:rofl2: Screw the man!


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Re: My SAB (Still Air Box) [Re: randoman]
    #21457047 - 03/25/15 09:03 PM (5 years, 6 months ago)

Yeah.People get away with that. If they look used, you can always just claim they suck. They don't really care, they just ahve to write something on the reason why.

They are a lot cheaper if you have an Harbor Freight or Northwest Tools around or some other cheapo hardware store.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #21459735 - 03/26/15 11:17 AM (5 years, 6 months ago)

In my experience Home Depot has a great return policy.  I bought a hammer drill and worked a week with it once and returned it no problems.  When I told them it wasn't adequTe for the job I was doing  they gave me my cash back no questions asked.  I was broke as shit back then. 

That's just my $0.02


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Re: My SAB (Still Air Box) [Re: Big Bear]
    #21461060 - 03/26/15 05:12 PM (5 years, 6 months ago)

That is definitely one of the benefits of big box stores.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #21662940 - 05/10/15 11:11 PM (5 years, 5 months ago)

I have been watching and I am quite new to this hobby, but has anyone tried to use large heavy duty balloons in an x or + pattern across the arm holes? they would certainly be tight enough around your arms, and then would seal up as your arms were removed. Balloons are cheap, replaceable, and would be tight enough after you removed your arms.
It is just a question. I think after reading this whole thread it is something I may try this week.
Thanks everyone for your great Ideas.


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Re: My SAB (Still Air Box) [Re: OldYamaha]
    #21663301 - 05/11/15 12:45 AM (5 years, 5 months ago)

Sounds like it might work but iirc the reason glove boxes went out of style was because of the vaccum the seal creates (along with restricted mobility) causes it to be less effective.  I'm not sure if that's 100% true but I do know that I haven't seen anyone who still uses one around here.  Your idea seems clever but a bit unnecessary.  I have 6" holes in mine and I very rarely get contams.  SABs are effective man.


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Re: My SAB (Still Air Box) [Re: Big Bear]
    #21664892 - 05/11/15 01:33 PM (5 years, 5 months ago)

I think your right, but I am very new, and trying to start as very cheaply, but safely as possible. I can build a flow hood, I am skilled that way, to just start out I want to be cheap. If I can make a little money that way, maybe my wife will get on board and not be a nag about it.


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Re: My SAB (Still Air Box) [Re: OldYamaha]
    #21665684 - 05/11/15 04:55 PM (5 years, 5 months ago)

SABs work.  A flowhood is unnecessary, especially if you are just growin a few cubes.  Find a box and cut some holes in it, then get to work :thumbup:


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Re: My SAB (Still Air Box) [Re: Big Bear]
    #21665737 - 05/11/15 05:07 PM (5 years, 5 months ago)

SABs can have big arm holes and still work. if you can't move around in your SAB think outside the box and build it bigger

Quote:

bodhisatta said:
Quote:

MudaFuka said:
I use a SAB inside a still air room


Quote:

MudaFuka said:
I added a completely clear viewing window to my SAB using weather stripping.









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Re: My SAB (Still Air Box) [Re: bodhisatta]
    #21666305 - 05/11/15 07:03 PM (5 years, 5 months ago)

I do all my sterile work in my SAB. I do everything from agar work to inoculating 9lb sawdust bags with grain spawn in that thing and my contam rate is very very low. I love my SAB.


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Re: My SAB (Still Air Box) [Re: MudaFuka]
    #21774815 - 06/07/15 06:04 PM (5 years, 4 months ago)

Would like to thank the op and all those that have participated in this thread, THANK YOU BROTHA'S! Oh and it was time for a bump.

:thumbup:


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Re: My SAB (Still Air Box) [Re: TrippieHunter]
    #21776830 - 06/08/15 02:21 AM (5 years, 4 months ago)

just know people you can do better... much better...


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Re: My SAB (Still Air Box) [Re: livelogikal]
    #21779410 - 06/08/15 05:47 PM (5 years, 4 months ago)

Quote:

livelogikal said:
just know people you can do better... much better...





Now that's not very positive.


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Re: My SAB (Still Air Box) [Re: TrippieHunter]
    #21779494 - 06/08/15 06:14 PM (5 years, 4 months ago)

For the price and effectiveness I have yet to see something better than an SAB, or else Id gladly switch over.  Every single noteworthy grower on here uses an SAB or flowhood (save a couple random shmuvbox users.)


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Re: My SAB (Still Air Box) [Re: livelogikal]
    #21781536 - 06/09/15 03:53 AM (5 years, 4 months ago)

Quote:

livelogikal said:
just know people you can do better... much better...




Oh fuck off.  This SAB design is as good as any, and better than most.  Hoods aren't the end-all of mycology either.


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #21781638 - 06/09/15 05:08 AM (5 years, 4 months ago)

Quote:

Inocuole said:
Quote:

livelogikal said:
just know people you can do better... much better...




Oh fuck off.  This SAB design is as good as any, and better than most.  Hoods aren't the end-all of mycology either.




Look at his post count. Probably having a hard time getting it up. :lol:


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Re: My SAB (Still Air Box) [Re: insanemike]
    #21781653 - 06/09/15 05:19 AM (5 years, 4 months ago)

Quote:

insanemike said:
Probably having a hard time getting it up. :lol:




Getting it up...
:hahthatsrich:

:growshrooms:


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #21781873 - 06/09/15 08:32 AM (5 years, 4 months ago)

The SAB is great.  I'm still sitting at a 98% clean spawn rate after hundreds of g2gs.  When I do get a contam, I'm pretty sure it is my sterile techniques fault; or a new thing I'm trying, not the SAB.  I haven't had any bad jars in a long time...

I plan to build a flowhood eventually, just because I like the idea of not having my arms restricted to the SAB tub walls.  I'll bet my contam rates would be just the same though, if not worse.  They are quite expensive vs a SAB, I just haven't been able to justify putting out the cash to build one as of yet for the small benefit it gives.

:chief:


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Re: My SAB (Still Air Box) [Re: TheChief]
    #21909602 - 07/07/15 02:48 PM (5 years, 3 months ago)

I made my SAB and added the magnetic hole covers, the magnets at Menards are 6.99 for 3. Also, with the Hole saw, if you run the drill bit in reverse the teeth will more melt than cut, and will dramatically decrease the possibility of cracking the plastic while cutting.
As a machinist for over 20 years, thats one of the tricks you learn.


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Re: My SAB (Still Air Box) [Re: OldYamaha]
    #21920814 - 07/09/15 06:49 PM (5 years, 3 months ago)

Nice. Excellent tip, too


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #21921227 - 07/09/15 08:28 PM (5 years, 3 months ago)

did you by chance save more money at menards?


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OfflineOldYamaha
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Re: My SAB (Still Air Box) [Re: bodhisatta]
    #21926231 - 07/10/15 11:18 PM (5 years, 3 months ago)

I am fortunate that in this city, we have 2 Lowes, 2 Menards, 1 Home Depot. So whoever has the lowest price gets my business.
I did get to pour my first agar in my new SAB, but I kinda made a mess. I let the agar cool to long, and got clumps, reheated it to melt in the microwave, poured too soon and darned near burned my fingers, used too much rubbing alcohol and almost asphyxiated my wife and I, put the SAB on the couch, and the petri dishes have agar on the lids, slopped out on the rims, and have a lot of condensation. but what the heck:smile:, it was my 1st pour. I think I will also add a clear plexglas windows too the SAB, it was just too fuzzy to see what I was doing in there, maybe also add some top lighting.
Now I am waiting to see if the condensation clears up, and if I have managed to contaminate any of the plates, but the 2 slants seem to be good.:takingnotes: I'll get better, can't see how it could be worse... well beside burning the house down. LOL


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #22081806 - 08/12/15 04:58 PM (5 years, 2 months ago)

Just wanted to thank SpitballJedi for explaining this SAB design so well. :thumbup:

I've tried to stick to it closely for my first pf-tek project. It was very helpful.

Made one very small variation: I am right handed, and during dry runs of the inoculations I found that I preferred my right hand to be a little higher than my left hand. So I made the right-hand hole a few cm higher than the left-hand hole. It worked great for me...

Love, Hanz.


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Re: My SAB (Still Air Box) [Re: Hanz]
    #22132314 - 08/23/15 08:37 PM (5 years, 2 months ago)

Sorry for the bump here but i want this in my threads, i didn't have a saw i used a knife.....my arm holes are square lol i hope this does not effect anything


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Re: My SAB (Still Air Box) [Re: newera]
    #22132369 - 08/23/15 08:47 PM (5 years, 2 months ago)

I used a stake knife to make my first SAB. The square holes worked just fine.


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Re: My SAB (Still Air Box) [Re: MudaFuka]
    #22132526 - 08/23/15 09:31 PM (5 years, 2 months ago)

Cool thanks....i'd post a pic but its' sort of embarrassing lol, i think it will do the job though, i only need it for agar (it's a 66 qt) and then i'll make a bigger one to do grains and what not in......i have gloves but no sleeves and can not for the life of me find my inoculation loop, also i've read some threads like stro's cleanign and isolating which are helpfull and all but it seems much different when i'm working with no pours...streaking seems to be a lot trickier


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Re: My SAB (Still Air Box) [Re: newera]
    #22132555 - 08/23/15 09:43 PM (5 years, 2 months ago)

Don'the be embarrassed. I've made some pretty ghetto SABs . I haven't used sleeves for over a year and my loop is just a twisted up guitar string.


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Re: My SAB (Still Air Box) [Re: MudaFuka]
    #22203820 - 09/07/15 08:49 PM (5 years, 1 month ago)

Man, you just sound so "twisted":crazy2:, Or are you just wired???:grin:
I dropped my SAB and made a SAR Still Air Room. It is a 6'X6'X6' 1/2" PVC pipe box wrapped in 4mil plastic sheeting, inside I have a small Hepa filter glued to a small plastic box with a 12v computer cooling fan sucking air thru the filter.
I put everything in the room, spray the room down with 10% bleach water, turn on the fan and go shower, put on a tyvec hooded suit, and then enter and sit getting it all set up and then do my transfers. seems to be working, and I have plenty of room for doing grain to grain from jars to bags.
If I do redo that room, I would make it a bit bigger, so I can put a small shelving unit to keep my grain bags in there. I also learned that using a heat sealer is NOT as good as just using a wire tie to close the bags.


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Re: My SAB (Still Air Box) [Re: OldYamaha]
    #22204383 - 09/07/15 10:42 PM (5 years, 1 month ago)

My SAB is in a SAR.


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Re: My SAB (Still Air Box) [Re: MudaFuka]
    #22328016 - 10/03/15 02:37 PM (5 years, 23 days ago)

Quote:

MudaFuka said:
My SAB is in a SAR.





I like the Inception set up!


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #22758575 - 01/09/16 08:51 PM (4 years, 9 months ago)

SBJ thanks for this. I just picked up the same tote at Wallyworld tonight for $10. I'll be using it for my SAB as well. I just read through this entire thread, and saw a couple things that may be worth adding to the tips section of the first post if you can.

I didn't note who said this;
Quote:


You can use a heat gun to make plastic that isn't crystal clear much more clear. Just point the heat gun at the area you want to be more clear, and keep moving the heat gun back and forth like you're blow drying your hair. Just be careful not to melt the plastic.




This tote is already pretty clear, but anyone know if an actual hairdryer would work?

This is based on one of your own later posts. I added a few things so it can stand on its own without the other post for context;
Quote:


If the SAB cracks when making arm holes, tape works fine as a fix. The thing doesn't need to be water proof or completely air tight. You just want to eliminate as much air movement as possible without creating any vacuums.





*edited to add the bin was $10


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Edited by myceliumEX (01/10/16 04:00 AM)


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Re: My SAB (Still Air Box) [Re: myceliumEX]
    #22758580 - 01/09/16 08:51 PM (4 years, 9 months ago)

I tried the heat gun on some plastic like he said and it just warped on me, didn't get any clearer.  :shrug:


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #22760050 - 01/10/16 03:33 AM (4 years, 9 months ago)

Quote:

Inocuole said:
I tried the heat gun on some plastic like he said and it just warped on me, didn't get any clearer.  :shrug:




That's disappointing to hear. How close did you hold the heat gun? Maybe it was to close? He mentioned to use it like drying hair. IIRC you are supposed to hold a hair dryer like 12 to 16 inches away.

Anyone else actually get this one to work?


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Re: My SAB (Still Air Box) [Re: myceliumEX]
    #22760075 - 01/10/16 03:44 AM (4 years, 9 months ago)

I cut mine with a dremmel then very ghettoly siliconed Plexiglas


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Re: My SAB (Still Air Box) [Re: LocN9ne]
    #22760119 - 01/10/16 04:05 AM (4 years, 9 months ago)

For others, look for a window winterizing kit. The ones that come with basically a sheet of clingwrap with a sticky strip around the perimeter. Cut the whole, place the "clingwrap" over the whole. Hit the film with a hair dryer, and it shrinks up drum tight. I saw this mentioned in a different thread once upon a time. Guy used it for a viewing port in his SAB to make agar work a bit easier.


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Re: My SAB (Still Air Box) [Re: myceliumEX]
    #22836579 - 01/29/16 12:40 AM (4 years, 8 months ago)

What is 70% ipa?


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Re: My SAB (Still Air Box) [Re: prescottfry]
    #22836582 - 01/29/16 12:41 AM (4 years, 8 months ago)

70% isopropyl rubbing alcohol


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Re: My SAB (Still Air Box) [Re: PussyFart]
    #22836677 - 01/29/16 12:58 AM (4 years, 8 months ago)

:whathesaid:


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #22836713 - 01/29/16 01:06 AM (4 years, 8 months ago)

So im making the airbox... Really a smart invention... But wont the needle run the risk of containing contams when you have to sterilize on outside??? And also, when you have to reinsert your arm, wont that create more problems of airflow and contams? I understand the heat pressure thing of not light inside sab... Lastly, once your arms are inside box, if youre moving the needle out of the holes in cap, in my case 4 since i got 16oz jars, doesnt that increase risk of contams within sab?


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Re: My SAB (Still Air Box) [Re: prescottfry]
    #22836993 - 01/29/16 02:44 AM (4 years, 8 months ago)

A SAB is probably the most common method for inoculations around. Most beginners have the same questions and fears, but this is a tried and true method for doing sterile work. This isn't something I just made up. It has been around and used by many others fr quite some time.

After you flame your needle red hot, it will stay hot enough to kill anything that lands on it until you are ready to inoculate as long as you move it directly to the SAB. Given the size of the needle, it's unlikely anything will land on it any way, especially if you if the air in the room is fairly still.

Yes, there is a slight disturbance of the air when you move in and out of the box and that's why you should move deliberately but slowly to prevent as much disturbance as possible. If you clean everything, use good techniques, and move with caution, you will not disturb the very few particles that may be present.

Good technique includes not putting your hands or tools or other equipment over the top of open sterile containers. Only what you have to to make transfers an inoculations. Put as little over an open sterile container as possible. You get better at this with practice.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #23064184 - 03/30/16 07:22 PM (4 years, 6 months ago)

:bumpthread:


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #23113522 - 04/13/16 04:07 PM (4 years, 6 months ago)

awesome write up, i will be creating one of these today


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Re: My SAB (Still Air Box) [Re: SpitballJedi] * 1
    #23400280 - 07/01/16 10:05 AM (4 years, 3 months ago)

This is a very helpful writeup! As someone who's been trying to figure out how to start using agar, with little confidence in SAB setup and technique, it gives me the insight and motivation to try agar. Thanks, SpitballJedi!


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Re: My SAB (Still Air Box) [Re: Citizen X]
    #23888059 - 12/02/16 05:47 PM (3 years, 10 months ago)

:popcorn:


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Re: My SAB (Still Air Box) [Re: IMBiff]
    #24027289 - 01/21/17 04:31 AM (3 years, 8 months ago)

walmart has the 1478 back in stock btw... finding the right one can be a little tricky I found... just melted some holes, came out real clean thx for the tips!


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Re: My SAB (Still Air Box) [Re: IMBiff]
    #24208421 - 03/31/17 04:35 PM (3 years, 6 months ago)

Quote:

IMBiff said:
This is a very helpful writeup! As someone who's been trying to figure out how to start using agar, with little confidence in SAB setup and technique, it gives me the insight and motivation to try agar. Thanks, SpitballJedi!




Same!

:crazy:


Excellent write up!:takingnotes:


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Re: My SAB (Still Air Box) [Re: MudaFuka]
    #24423161 - 06/21/17 10:12 AM (3 years, 4 months ago)

Yeah...resurrecting the ancient like an archaeologist!
I am getting ready to execute an idea similar to this one. A SAB in a SAR is a great idea! One can hepa the air, go in, shut off hepa, wait five minutes, then....get it done!

Oh, this is reference to a post from last year or so where a still air area was set up to work in with the SAB. The original post didn't quote in mine, so sorry it looks out of left field! Has anyone used this idea? Overkill (assuming that's possible in this hobby)? I looked up SAR in the search function...who knew there were so many posts about SARS but not Still Air Rooms?


Edited by TravellinCrudge (06/21/17 10:15 AM)


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Re: My SAB (Still Air Box) [Re: TravellinCrudge]
    #24423261 - 06/21/17 11:16 AM (3 years, 4 months ago)

A still air room is unnecessary.


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Re: My SAB (Still Air Box) [Re: bodhisatta]
    #24423397 - 06/21/17 12:18 PM (3 years, 4 months ago)

i have floor vents so i throw a magazine over the floor vent prep the box, smoke a bowl, piss. put on groovy tunes, gloves. go to work.

air is as still as it needs.


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Re: My SAB (Still Air Box) [Re: mushboy]
    #24574520 - 08/23/17 05:18 PM (3 years, 2 months ago)

Size that really matter? Can i use 40q box?


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Re: My SAB (Still Air Box) [Re: Gunsel]
    #24574553 - 08/23/17 05:33 PM (3 years, 2 months ago)

40q is pretty small would be hard to work in


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Re: My SAB (Still Air Box) [Re: bodhisatta]
    #24578361 - 08/25/17 08:24 AM (3 years, 1 month ago)

Nice, I like how you put the magnetic tape over the holes. That makes it super convenient.


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Re: My SAB (Still Air Box) [Re: TripSwaddo]
    #24637547 - 09/17/17 12:07 PM (3 years, 1 month ago)

Well written !!!

will keep all the details in mind !!!

specially like the way to check for contamination in SAB .


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Re: My SAB (Still Air Box) [Re: oy13]
    #24649444 - 09/21/17 05:54 PM (3 years, 1 month ago)

Is an SAB absolutely necessary for this step or is it ideal? If done in a small space with no air flow and carefully could this step be done without the SAB? Also, how is the environment remaining sterile if you are going into and out of it? Thanks for your help. This is my first time :smile:


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Re: My SAB (Still Air Box) [Re: deeswpr19]
    #24649466 - 09/21/17 06:01 PM (3 years, 1 month ago)

Absolutely necessary?  Pretty much.  Do yourself a favour and build one.  Its a rubbermaid tub with a handhole cut in the side and another in the front, and saran wrap taped over a big hole in the lid(so you can see).  At least mine was that.

Clean it out before each use and spray the inside with lysol too.


--------------------
 

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Edited by VroomerMcZoomers (09/21/17 06:14 PM)


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #25342095 - 07/23/18 12:30 PM (2 years, 3 months ago)

Hi,
I'm a newbie reading many different TEKs when I found this.

I've checked the glossary and abbreviation lists, but no answer to:
What is "SHIPs?"  When I do a search it comes up with posts about shipping.
What is "petris?"

Thank you,
Paisley


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Re: My SAB (Still Air Box) [Re: paisley777]
    #25342119 - 07/23/18 12:45 PM (2 years, 3 months ago)

Self healing injection port

Petris are petri dishes.


--------------------
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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #25659618 - 12/05/18 05:59 PM (1 year, 10 months ago)

Quote:

SpitballJedi said:
Some people heat up a coffee can to melt the holes too.




We don't seem to have the type of coffee cans here in the UK as you have in you have in the US. Here coffee is mainly sold in bags. But I used a large dog food can and it worked a treat, made a really neat hole. The only downside was I needed to heat up the can a few times because it didn't melt right the way through on the first attempt, but it was definitely quicker and easier than cutting or sawing, and less chance of f***ing it up.


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Re: My SAB (Still Air Box) [Re: Duggstar]
    #25659625 - 12/05/18 06:01 PM (1 year, 10 months ago)

I used a menudo can


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InvisiblebodhisattaM
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Re: My SAB (Still Air Box) [Re: JHOVA]
    #25659631 - 12/05/18 06:02 PM (1 year, 10 months ago)

Juanita's hominy cans work too


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Re: My SAB (Still Air Box) [Re: bodhisatta]
    #25659641 - 12/05/18 06:04 PM (1 year, 10 months ago)

Thats exactly what i used


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Re: My SAB (Still Air Box) [Re: JHOVA]
    #25659745 - 12/05/18 06:35 PM (1 year, 10 months ago)

Thanks for the suggestions guys, but I was really commenting for the benefit of people in the UK. As I have found, it's really hard to find large enough cans in the UK as people prefer to buy multipacks of single serving cans rather than one large can, because it keeps fresher I guess. I almost gave up until I checked the pet food isle. I don't even have a dog, but fuck it, it only cost a £1. :lol:


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Re: My SAB (Still Air Box) [Re: Duggstar]
    #25668099 - 12/09/18 04:30 PM (1 year, 10 months ago)

Thanks for sharing your Tek
keen to give it a go!


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #26049045 - 06/12/19 10:52 PM (1 year, 4 months ago)

Thanks for all of the great info!! Bookmarked!


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Re: My SAB (Still Air Box) [Re: weedjee]
    #26058249 - 06/17/19 09:57 PM (1 year, 4 months ago)

whaddaya think? didnt put the holes in yet but i think this tubll work great it fits so snug on top



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Re: My SAB (Still Air Box) [Re: yeetgodtwelly]
    #26058268 - 06/17/19 10:10 PM (1 year, 4 months ago)

is that table large enough to cover any overhang from the box?

opened bottomed SAB=mold ime. i tried it on a wire shelf for awhile. didnt work too well.


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Offlineyeetgodtwelly
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Re: My SAB (Still Air Box) [Re: mushboy]
    #26058326 - 06/17/19 10:40 PM (1 year, 4 months ago)

there is like an inch air gap on the sides, is there anything i can cover that open bottom with?


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Re: My SAB (Still Air Box) [Re: yeetgodtwelly]
    #26058344 - 06/17/19 10:47 PM (1 year, 4 months ago)

use a larger table.

i suppose you could use saran wrap or something but thatd be ghetto as f


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Re: My SAB (Still Air Box) [Re: mushboy]
    #26058399 - 06/17/19 11:21 PM (1 year, 4 months ago)

true /: im happy i got your advice on this one mushboy, thanks.


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Re: My SAB (Still Air Box) [Re: mushboy]
    #26084221 - 07/01/19 02:54 PM (1 year, 3 months ago)

about to get started tn :cool:



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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #26124681 - 08/06/19 08:47 PM (1 year, 2 months ago)

Great TEK!

I'm a total (with some caveats) nooby here and
I'm so impressed that I had to say THANK YOU".

You obviously put a lot of effort into this,
and I really appreciate it.

As for the caveats; I'm a retired Engineer, and probably
on the OCD spectrum.

I think I'll read it again now, bye.....


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