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InvisibleSpitballJedi
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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20847334 - 11/16/14 08:41 PM (5 years, 11 months ago)

I updated the OP with the following:


Getting Ready For Doing Work

I lay the towel down on the table. I have large bath sized towels, so I fold it over. Then, I spray the towel with soap water. I usually use Dr Bronner's Almond Scented Magic Soap. It's what I shower with, but any soap will do. It doesn't take much, just a couple of drops per liter of water. The soap just keeps the water from evaporating too quickly and gives it a little stickiness. I don't see the point in sanitizing the table because it's going to have a towel over it, but do it if it makes you feel good.

Then, I clean the walls of the SAB with 70% IPA. After that, I give a spritz of soap water on the walls and turn the SAB upside down on the towel. Contaminants will stick to wet surfaces. I find soapy water works best. Bleach irritates my nose and lungs. Alcohol dries fast and is flammable.

You can either have the jars or whatever on the towel and cover with the box or feed everything through the arm-holes; it doesn't matter at all.

Once my stuff is in, I let everything just settle for a few minutes, maybe 2-5 minutes. Then, I wipe all my jars or whatever with 70% IPA.

Tips


    As Already mentioned, the point of a SAB is to have a place of still air to do sterile work. We call it sterile work because you want to keep your sterilized stuff sterile. The inside of the SAB is not sterile, just still. I put the SAB in the least drafty room, close the door, and turn off anything that creates drafts or air circulation.

Let things settle before wiping down jars in your SAB.

    I wear nitrile exam gloves and tyvek sleeves. I actually wear the top half of a tvek suit with the legs cut off. You can just wear a clean long-sleeve shirt though. When not in use, I keep the tyvek in a plastic bags just to keep the dust off. I actually keep a lot of stuff wrapped up in bags or totes to keep the dust off.

    Do all flame sterilizations outside the SAB. Heat from the flame creates enough current inside the SAB to cause contams to float around and get in to your sterile work.

    It's a fact, attaching gloves to your SAB turns it in to a Glove-box. Attaching the gloves causes more air to move around so therefore, by definition, it is no longer a Still Air Box. I'm sure there are all kinds of ways to prevent contaminated air from coming in to your GB, but you better make sure the inside is already sterile or it will be pointless. Good luck getting it sterile.

    Do your work toward the back of the SAB to help prevent contams from getting in your sterile work via the arm holes.

    Work diligently. Don't get in a hurry or work at a snails pace. Make all your movements deliberately. Getting in a hurry can stir up the air inside. Working too slow leaves you open for higher potential of getting contams.

Crack the lids on jars before you put them in the SAB. A stuck lid is a bitch to open inside it.

You don't have to shower the walls. You just want a little spritz. Too much, especially in front, will make it hard to see what you're doing. If you are doing much work or working with warm agar, you'll notice condensation building up on the walls anyway. There shouldn't be many contams floating around and it doesn't take much to trap them.

Keep movement over open sterile containers at a bare minimum. I don't like to sit anything dow. This takes a little practice, planning, and dexterity. Try dry runs without any media or anything to see how it feels. Just use some empty jars, bottles, and petris.

One last thing: Always flame sterilize outside the SAB


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20848040 - 11/16/14 11:18 PM (5 years, 10 months ago)

Good addition :highfive1:

I couldn't agree more with cracking the lids before working. I've remade my lids upside down though so this isn't a problem, but I do find it harder to keep the lids from sliding around on the jar this way.


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Re: My SAB (Still Air Box) [Re: wowimflabbergasted]
    #20848115 - 11/16/14 11:37 PM (5 years, 10 months ago)

What I meant by cracking the lid was actually unscrew it a little. I guess I'll clarify that in the OP.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20848119 - 11/16/14 11:38 PM (5 years, 10 months ago)

Ah okay I got ya. Cracking the lids outside the SAB isn't a good idea lol that's why I quit.


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Re: My SAB (Still Air Box) [Re: wowimflabbergasted]
    #20848127 - 11/16/14 11:42 PM (5 years, 10 months ago)

I pull my rings all off once they're inside the SAB and then unseal the lid of the jar(s) I'm about to work with, is there anything wrong with that?


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Re: My SAB (Still Air Box) [Re: wowimflabbergasted]
    #20848137 - 11/16/14 11:46 PM (5 years, 10 months ago)

:lol: Yeah, I clarified. I don't use the 2-piece metal ones anymore, so I forgot. Good call and thanks for bringing it up. I wonder how many noobs would be cracking the lid part and trying to fogure out where the contams were coming from.

Quote:

SBJ said: Crack the lids.




Here's the update to the OP:

Crack the lids on jars before you put them in the SAB. A stuck lid is a bitch to open inside it. What I mean is, barely unscrew the metal ring or plastic lid so you don't have to fight it inside the box. If using the 2 piece metal lids, don't try to break the seal on the lid part, just barely loosen the ring. Once they are all wiped down and I'm ready to actually start working, I like to loosen all the lids so that I can finish removing them with one hand.


Edited by SpitballJedi (11/16/14 11:50 PM)


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20848142 - 11/16/14 11:48 PM (5 years, 10 months ago)

Excellent, you use the plastic lids then?


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #20848161 - 11/16/14 11:55 PM (5 years, 10 months ago)

Quote:

Inocuole said:
I pull my rings all off once they're inside the SAB and then unseal the lid of the jar(s) I'm about to work with, is there anything wrong with that?




Sounds right. I just wasn't thinking when I posted. Sorry for the confusion.

And yes, I use plastic now. It's awesome. Lids


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #20848652 - 11/17/14 02:28 AM (5 years, 10 months ago)

Quote:

Inocuole said:
Well I don't have a hole saw so I may have to figure something out instead.  Someone mentioned using a soldering iron but I really actually have to use that to solder sometimes... I suppose I could just cut the circles out with a hot blade.




I traced arm holes then cut them out with a dremel. Worked fine for me just took a little while :shrug:.


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Re: My SAB (Still Air Box) [Re: ToxicDuck]
    #20848662 - 11/17/14 02:33 AM (5 years, 10 months ago)

Quote:

ToxicDuck said:
Quote:

Inocuole said:
Well I don't have a hole saw so I may have to figure something out instead.  Someone mentioned using a soldering iron but I really actually have to use that to solder sometimes... I suppose I could just cut the circles out with a hot blade.




I traced arm holes then cut them out with a dremel. Worked fine for me just took a little while :shrug:.



Be careful of the fumes when melting plastic. That shit is horrible for you. Very...Toxic  :biggrin:


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Re: My SAB (Still Air Box) [Re: wowimflabbergasted]
    #20848754 - 11/17/14 03:17 AM (5 years, 10 months ago)

Quote:

wowimflabbergasted said:
Quote:

ToxicDuck said:
Quote:

Inocuole said:
Well I don't have a hole saw so I may have to figure something out instead.  Someone mentioned using a soldering iron but I really actually have to use that to solder sometimes... I suppose I could just cut the circles out with a hot blade.




I traced arm holes then cut them out with a dremel. Worked fine for me just took a little while :shrug:.



Be careful of the fumes when melting plastic. That shit is horrible for you. Very...Toxic  :biggrin:





Pshh, that'd bother me if I was a pussy. But as you can tell, I'm not a cat, I'm a fucking duck.

Ducks don't give a shit about anything.
Ducks will fuck you up.

:lol:.


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Re: My SAB (Still Air Box) [Re: wowimflabbergasted]
    #20848762 - 11/17/14 03:21 AM (5 years, 10 months ago)

Ugh I was trying to cut the circles in mine and it fucking cracked.  One 13 dollar tub down the shitter.  Well sorta.  I still gotta use it for now, so I put some clear industrial adhesive on it to form a seal.  :shrug:

I guess before I do my next one I have to invest in a big ass hole saw.


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #20848765 - 11/17/14 03:23 AM (5 years, 10 months ago)

Quote:

Inocuole said:
Ugh I was trying to cut the circles in mine and it fucking cracked.  One 13 dollar tub down the shitter.  Well sorta.  I still gotta use it for now, so I put some clear industrial adhesive on it to form a seal.  :shrug:

I guess before I do my next one I have to invest in a big ass hole saw.





You can still crack it with a hole saw, so you still gotta be careful. Go slow,  and have some wood pushed up behind the part you're drilling.


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Re: My SAB (Still Air Box) [Re: ToxicDuck]
    #20848766 - 11/17/14 03:24 AM (5 years, 10 months ago)

Well next time I go to cut it I'm going to point my heat gun at it for a couple seconds to soften it up real good.  That should help right?


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #20848798 - 11/17/14 03:54 AM (5 years, 10 months ago)

Quote:

Inocuole said:
Well next time I go to cut it I'm going to point my heat gun at it for a couple seconds to soften it up real good.  That should help right?





That should help a bit. Since you have a heat gun you probably already know this, but figure I'd say it for anyone else who also has one.


You can use your heat gun to make plastic that isn't crystal clear much more clear. Just point the heat gun at the area you want to be more clear, and keep moving the heat gun back and forth like you're blow drying your hair.


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Re: My SAB (Still Air Box) [Re: ToxicDuck]
    #20848801 - 11/17/14 03:57 AM (5 years, 10 months ago)

That's a pretty fuckin neat tip, I may try it around my viewing area so I have a place I can put my eyes to see real good. 
:vaped:


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Re: My SAB (Still Air Box) [Re: Inocuole]
    #20848848 - 11/17/14 04:32 AM (5 years, 10 months ago)

Quote:

Inocuole said:
That's a pretty fuckin neat tip, I may try it around my viewing area so I have a place I can put my eyes to see real good. 
:vaped:




It helps a lot. Just be careful not to melt the plastic :smile:.


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Re: My SAB (Still Air Box) [Re: ToxicDuck]
    #20849764 - 11/17/14 12:02 PM (5 years, 10 months ago)

Wow good tip Duck, I'll try it and see if it helps. Sometimes it's hard to see in there.

You can also use a coffee can to make the holes. Take an empty coffee can, the bigger ones, heat it up on the stove and push it into the tote where you want your arm holes.


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Re: My SAB (Still Air Box) [Re: ToxicDuck]
    #20849775 - 11/17/14 12:05 PM (5 years, 10 months ago)

Hole-saws can be found for pretty cheap. You can also run the hole-saw in reverse so it doesn't catch and crack the plastic.


Some people heat up a coffee can and melt the holes. I've never tried it though.

If it cracks, tape works fine as a fix. The thing don't need to be water proof or completely air tight. You just want to eliminate as much air movement as possible without creating any vacuums.


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Re: My SAB (Still Air Box) [Re: SpitballJedi]
    #20851148 - 11/17/14 05:10 PM (5 years, 10 months ago)

I applied clear industrial adhesive to both sides of the crack and it's pretty much sealed up now, just gotta get on with the arm hole cutting today.  I would do it at night but all the drilling and noise I make probably pisses off the neighbors.

My original design involved cutting 5  1 1/4 inch holes (one in the center, 4 in compass directions) so that I have the general outline of a circle shape already there.  Just gotta cut between the circles.  Somehow I fucked that up too, so heat gun it is, today.

Also my coffee is in a bag.  :shrug:


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