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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Lids 9
#20048747 - 05/27/14 08:16 PM (9 years, 7 months ago) |
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This is part of my series "The Basics"
This thread will address the 2-part metal lids and 1-piece plastic lids for glass wide-mouth jars.
After you put grains in your jars to be sterilized, it's time for the lids.
Before reading any further, I do not use SHIPs. I open my jars in a SAB for all inoculations. Look hear to findout why: SHIPs vs Opening Lid Thread Do not poke a hole in your filter. For other lid types, you will need to use the search feature.
You can use these lids with any size jar or any tek, including PF/BRF cakes. You can skip the dry vermiculite contam barrier and just use a filtered lid instead.
I sometimes use cakes to test cultures before I commit to a larger grow. I use these lids. Just open in a SAB, inject, close. Proceed as usual without the fear of moving the jar around.
Metal Lids:
The metal lids come in 2 parts: lid and ring
Using ay drill and 3/32" drill bit, I put 4 GE holes in the lids. During colonization, the mycelium needs to breath. CO2 build up is good for mycelium growth, but too much will cause stalling. 1/16" holes seem too small and growth stalls a little. 1/8" holes seem too big and my grains dry out a little. But anything from 1/16 to 1/8 just fine for most people.
Make sure you get rid of the burrs from making holes so they won't etch in to your SFD

I put on lid, SFD, ring, then foil…in that order.
   
I put the lids on upside down so they are easy to remove. The picture shows the lid on with seal down like normal because the pic was taken before I started flipping upside down.
The SFD does an excellent job of allowing GE while preventing contaminations from getting in. SFD's are the gold standard for filters on jars. The ring keeps the lid on. Duh The foil prevents water from raining down on your jars during the PC process. This could get inside the jar and make the grains too wet. Many say you don't need foil when using an SFD, but I do anyway because that's what RR does. I like to leave the foil on until I'm ready to put in my SAB.
7 jars

Plastic lids:
Plastic lids are one piece. I put a 1/4" hole in the middle.
I stick a whole SFD in the underside of the lid, then, screw the lid on just kind of snug, but not too tight. Then cover with foil.
Lid
Lid with SFD in it
Lid on Jar
With foil on top
Plastic Lids v.2:
Here's how to make and use a plastic lit with small SFD RTV'd on top.
You can get 7 filters from a single wide-mouth size SFD. Use a quarter and pen to trace out 7 circles and then cut them all out with scissors.
 
Use a piece of sandpaper to scruff up the middle of your lid. This will help the RTV stick better. Then, use a quarter to trace a circle in the middle. This will help you know where to put the hole and RTV without getting so messy.
Drill a 1/4" hole in the middle of the circle.
Traced circle. I used a Sharpie with drilled hole. After you've done a few of these, tracing the circle becomes unnecessary because you will be able to just eyeball it.
RTV the inside of the circle in a narrow bead.
Then, gently place the cut out SFD over the hole and RTV. Push down directly in the middle and roll your finger to the outside edges of the filter. This will help push down the edges for the seal without pushing RTV to the middle and clogging the hole.

Finished
From underneath.
You can cover in foil and PC directly after if you want. The RTV will cure during the cycle. Just don't mash the foil down while wet or it may stick to the lid or move the filter around and break the seal.
I used the blue RTV because that"s what they had. Use any high temperature RTV or silicone with a heat rating over 300F. Your PC is 250F at 15 psi.
 
The foil is also optional with SFD's.
Time for the Pressure Cooker
Edited by SpitballJedi (11/23/14 05:30 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,042
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Quote:
SpitballJedi said: The ring keeps the lid on. Duh
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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nucleophile
international cowboy chemist


Registered: 04/28/13
Posts: 299
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Quote:
SpitballJedi said:
Lid on Jar
With foil on top
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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amanita virosa does plastic lids like that, with SFD under lid. so in this instance, are we no concerned with filter getting wet during shaking?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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Quote:
blindingleaf said: amanita virosa does plastic lids like that, with SFD under lid. so in this instance, are we no concerned with filter getting wet during shaking?
I think the SFD are pretty water resistant, for it to turn into a contam super highway I think the moisture has to soak all the way through. If the top of the SFD is still dry, there's no way for the contam to travel down into the jar.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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The filter is dry and the grains are not really wet enough to get the filter wet.
I've only been using plastic lids for a few runs, but it seem to be working fine. I got the idea from Aminita Virosa who has done this countless times.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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billb
The Wolf I Feed



Registered: 10/09/11
Posts: 1,040
Loc: Dancing with the Dead in my Dr...
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I've found that using the plastic lids more then a few times warps them and makes it a pain the ass for g2g in a sab, but for straight grain nocc they sure look nice sitting on the shelf.
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Quote:
SpitballJedi said:
Lid on Jar
With foil on top
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Some people need pictures
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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midnightmaraude
Stranger


Registered: 05/09/14
Posts: 1,260
Last seen: 7 years, 1 month
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So...do you cover the inoculation point with micropore tape or anything?
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toxetel
׀ǝʇǝxoʇ ●


Registered: 08/13/12
Posts: 708
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Inoculation point?
These lids are meant to be removed to inoculate the grain.
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Quote:
midnightmaraude said: So...do you cover the inoculation point with micropore tape or anything?
They already have a Synthetic Filter Disk GE filter, no need for more GE filters. You can inoculate with syringes or agar wedges,g2g.
Edited by Mr. Alien (07/11/14 02:21 PM)
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Quote:
midnightmaraude said: So...do you cover the inoculation point with micropore tape or anything?
Quote:
toxetel said: Inoculation point?
These lids are meant to be removed to inoculate the grain.
Correct. I open the lids in a SAB for inoculations.
I don't use SHIPs anymore.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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nucleophile
international cowboy chemist


Registered: 04/28/13
Posts: 299
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What kind of plastic lids have you been using? I keep finding mixed information about how well they work compared to metal lids and people claiming some kinds can warp slightly and allow unsterile air in. I know they don't always make a totally watertight seal on a plain jar with no holes and a filter. I've used the Ball ones a few times with no problems, though, so I'm guessing the issues might be overstated.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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I use Ball, in the green box. I haven't had many runs with them yet, so the jury is still out.
You don't need a water-tight seal. Air will follow the path of least resistance, this path is the GE hole. The SFD under the plastic lid creates a seal and there is no way a contam is gonna get between the glass lip and filter.
I just snug the lid a little before they go in the PC. I think over-tightening before PCing or while still hot may cause some of the warping.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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shamanamba
The Shaman



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-------------------- The Shaman
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fatflash
Commodore


Registered: 01/15/13
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So is there a tek on how to make lids
Edited by fatflash (07/10/14 06:35 PM)
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